FastQCFastQC Report
Wed 25 May 2016
SRR2431430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524753
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA257410.30195596282965614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94700.045.6162951
GTATCAA158650.033.776861
ATCAACG171200.030.6404083
TCAACGC171900.030.2421954
TATCAAC200250.026.4536442
CAACGCA202300.025.6976455
GTACATG336900.024.4309081
AACGCAG214550.024.1410736
TACATGG329650.023.6267242
CGCAGAG218650.023.172368
ACGCAGA222500.022.9192667
ACATGGG337500.021.8794563
GTGGTAT50650.021.4381371
TGGTATC50100.020.0775222
CATGGGT74750.020.0591984
GCAGAGT253700.018.9706849
CAGAGTA246550.017.29032910-11
CATGGGG165550.016.8368024
CATGGGA139600.016.431174
AGAGTAC233850.016.06874810-11
Produced by FastQC (version 0.11.5)