##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431430_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8524753
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.324404941703296	31.0	31.0	34.0	26.0	34.0
2	30.368839073695156	31.0	31.0	34.0	26.0	34.0
3	30.45807919596028	31.0	31.0	34.0	26.0	34.0
4	33.85182327276814	37.0	35.0	37.0	28.0	37.0
5	33.952721328113554	37.0	35.0	37.0	30.0	37.0
6	33.98539429822776	37.0	35.0	37.0	30.0	37.0
7	33.8530404341334	37.0	35.0	37.0	29.0	37.0
8	33.814346057885786	37.0	35.0	37.0	29.0	37.0
9	35.198371729949244	39.0	35.0	39.0	28.0	39.0
10-11	35.145470842381	39.0	35.0	39.0	28.0	39.0
12-13	35.113933858259585	39.0	35.0	39.0	27.0	39.0
14-15	36.31326315260982	40.0	37.0	41.0	27.0	41.0
16-17	36.32813707329702	40.0	37.0	41.0	27.0	41.0
18-19	36.33053104295222	40.0	36.5	41.0	27.0	41.0
20-21	36.42106832890056	40.0	37.0	41.0	27.5	41.0
22-23	36.37668282001836	40.0	37.0	41.0	27.5	41.0
24-25	36.400540754670544	40.0	36.5	41.0	28.0	41.0
26-27	36.36992614331465	40.0	36.0	41.0	28.5	41.0
28-29	36.257001229243826	40.0	36.0	41.0	28.0	41.0
30-31	36.22689865618394	40.0	36.0	41.0	28.0	41.0
32-33	36.170024632971774	40.0	36.0	41.0	28.0	41.0
34-35	36.07181920696119	40.0	36.0	41.0	27.5	41.0
36-37	35.958269289444516	40.0	36.0	41.0	27.0	41.0
38-39	35.86683197741917	40.0	36.0	41.0	27.0	41.0
40-41	35.78580341272058	40.0	35.0	41.0	27.0	41.0
42-43	35.584591013956654	40.0	35.0	41.0	26.0	41.0
44-45	35.47045304421137	40.0	35.0	41.0	26.0	41.0
46-47	35.34499357342084	39.0	35.0	41.0	26.0	41.0
48-49	35.21814626183304	39.0	35.0	41.0	26.0	41.0
50-51	35.06179592534822	39.0	35.0	41.0	25.0	41.0
52-53	34.978491224320514	39.0	34.5	41.0	25.0	41.0
54-55	34.90983486559669	39.0	34.0	41.0	25.0	41.0
56-57	34.751783482758974	39.0	34.0	41.0	24.5	41.0
58-59	34.547001244493536	38.5	34.0	41.0	24.0	41.0
60-61	34.33802433923892	38.0	34.0	40.0	23.5	41.0
62-63	34.08404466381607	38.0	33.5	40.0	23.0	41.0
64-65	33.95456812649	37.5	33.5	40.0	23.0	41.0
66-67	33.70352114600857	37.0	33.0	40.0	22.0	41.0
68-69	33.45037392872263	37.0	33.0	40.0	22.0	41.0
70-71	33.16402281684877	36.0	33.0	39.0	21.5	41.0
72-73	32.85918688787815	36.0	33.0	39.0	20.5	41.0
74-75	32.51911251856799	35.0	32.0	39.0	20.0	40.5
76-77	32.184973042620705	35.0	32.0	37.5	19.0	40.0
78-79	31.83421760137801	35.0	32.0	37.0	18.0	39.0
80-81	31.513753888235826	35.0	32.0	37.0	16.0	39.0
82-83	31.188062926867204	35.0	32.0	36.0	12.0	39.0
84-85	30.877390171891197	35.0	31.0	36.0	7.5	37.0
86-87	30.600809607034947	35.0	31.0	35.5	4.5	37.0
88-89	30.31121112834589	35.0	31.0	35.0	2.0	37.0
90-91	30.069134026522526	35.0	31.0	35.0	2.0	36.0
92-93	29.863644377731532	35.0	31.0	35.0	2.0	36.0
94-95	29.622466246236108	34.5	31.0	35.0	2.0	36.0
96-97	29.50723082533887	35.0	31.0	35.0	2.0	36.0
98-99	29.336917503650838	35.0	31.0	35.0	2.0	35.0
100	29.19465596246601	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4072714192687634
1101	2	0.5097421700318243
1101	3	0.48749257807061497
1101	4	0.49149394595699647
1101	5	0.3685835941759521
1101	6	0.3784224316767819
1101	7	0.43465904960063284
1101	8	0.5165854544221773
1101	9	0.6188645368557673
1101	10-11	0.6657349245668271
1101	12-13	0.6219502277348994
1101	14-15	0.7888520407710331
1101	16-17	0.7590591210440749
1101	18-19	0.7083270420356484
1101	20-21	0.6968717385895431
1101	22-23	0.659542759716139
1101	24-25	0.7142218834434573
1101	26-27	0.7450712927085448
1101	28-29	0.764409466831836
1101	30-31	0.7525719883474977
1101	32-33	0.7173964079303516
1101	34-35	0.7148878339716944
1101	36-37	0.7461030983384873
1101	38-39	0.7141388129591277
1101	40-41	0.6471181070293284
1101	42-43	0.6502696415938587
1101	44-45	0.6267819026974237
1101	46-47	0.569760389109689
1101	48-49	0.5867556682605013
1101	50-51	0.5655497682878021
1101	52-53	0.5609009379028578
1101	54-55	0.6200747562093767
1101	56-57	0.6067313945166148
1101	58-59	0.6347684946094247
1101	60-61	0.6111063032882811
1101	62-63	0.6429743998786961
1101	64-65	0.6036024497062371
1101	66-67	0.626300607938397
1101	68-69	0.5888887394162694
1101	70-71	0.6140962146287521
1101	72-73	0.5587330184498072
1101	74-75	0.5303200386008058
1101	76-77	0.5763830817707074
1101	78-79	0.5360338764118815
1101	80-81	0.5455573859762879
1101	82-83	0.5263187286090272
1101	84-85	0.5039125523304584
1101	86-87	0.5275558869353745
1101	88-89	0.5570631829454413
1101	90-91	0.5344779593030147
1101	92-93	0.543939813354072
1101	94-95	0.5694591948702268
1101	96-97	0.5853356964577188
1101	98-99	0.6023393808742838
1101	100	0.5587600788485858
1102	1	0.3337092588571906
1102	2	0.3891783141220948
1102	3	0.3532475092363292
1102	4	0.35093178454276597
1102	5	0.3166113785545761
1102	6	0.38396881289281737
1102	7	0.39518407248635157
1102	8	0.43655922817728054
1102	9	0.5278093006474407
1102	10-11	0.5039035860256789
1102	12-13	0.4915812755714768
1102	14-15	0.5755829044189511
1102	16-17	0.5088083306972919
1102	18-19	0.5192395178339453
1102	20-21	0.49797481505139274
1102	22-23	0.5306159251573916
1102	24-25	0.557309469810086
1102	26-27	0.5882428518549574
1102	28-29	0.6421026953002951
1102	30-31	0.5961210490054185
1102	32-33	0.6477160877074724
1102	34-35	0.5993137993912896
1102	36-37	0.554549448171251
1102	38-39	0.466043658180169
1102	40-41	0.5152880284976504
1102	42-43	0.5511828894404047
1102	44-45	0.4830015367198186
1102	46-47	0.4551929416716334
1102	48-49	0.4406568180107868
1102	50-51	0.4226250473364601
1102	52-53	0.4442952914561644
1102	54-55	0.4848468298540851
1102	56-57	0.48436266694903907
1102	58-59	0.5137122908341425
1102	60-61	0.4688029809073342
1102	62-63	0.4544418897961151
1102	64-65	0.45629923761790536
1102	66-67	0.47697486340481987
1102	68-69	0.5034567132508485
1102	70-71	0.5200346693491227
1102	72-73	0.5589555254274856
1102	74-75	0.5475551383203694
1102	76-77	0.472924481103135
1102	78-79	0.43124716894363146
1102	80-81	0.4458506974650014
1102	82-83	0.4095999486858908
1102	84-85	0.3902027134612709
1102	86-87	0.39514180069792104
1102	88-89	0.4442433719869676
1102	90-91	0.42403123581225444
1102	92-93	0.3827084415246098
1102	94-95	0.4369787659589903
1102	96-97	0.4255484057755403
1102	98-99	0.44091545653634867
1102	100	0.3914488688751909
1103	1	0.32741352788184486
1103	2	0.37630856185086614
1103	3	0.4068654670250673
1103	4	0.40615205183910064
1103	5	0.3662630443790249
1103	6	0.476155636982341
1103	7	0.47867239070397716
1103	8	0.4834142912368051
1103	9	0.5737672430999368
1103	10-11	0.6652074109589918
1103	12-13	0.5352612996345698
1103	14-15	0.6869451386088059
1103	16-17	0.599552577380507
1103	18-19	0.4582577459815411
1103	20-21	0.5718202194952937
1103	22-23	0.5056038007965213
1103	24-25	0.5555214516804909
1103	26-27	0.5716351879252812
1103	28-29	0.6002563566542278
1103	30-31	0.501697539944594
1103	32-33	0.587535558346211
1103	34-35	0.5620429976911865
1103	36-37	0.6002839173422174
1103	38-39	0.6120772903857556
1103	40-41	0.5670248224324723
1103	42-43	0.5793635163655821
1103	44-45	0.54858829079744
1103	46-47	0.5154500343992439
1103	48-49	0.5291382308607737
1103	50-51	0.5168559165251239
1103	52-53	0.5205560454380631
1103	54-55	0.6002093949641321
1103	56-57	0.5500982047608431
1103	58-59	0.5649880751630647
1103	60-61	0.5812921636762169
1103	62-63	0.5235076238298575
1103	64-65	0.5760029493600172
1103	66-67	0.6152960258764963
1103	68-69	0.558443088456066
1103	70-71	0.5066852601529703
1103	72-73	0.51844268786855
1103	74-75	0.5177637665686774
1103	76-77	0.5190071547237096
1103	78-79	0.554455270003313
1103	80-81	0.5479683861570379
1103	82-83	0.5540448456043521
1103	84-85	0.529494683374832
1103	86-87	0.4891037987965987
1103	88-89	0.5638152207683476
1103	90-91	0.5931164418960613
1103	92-93	0.529963126877373
1103	94-95	0.5326054422827831
1103	96-97	0.5676611876939575
1103	98-99	0.5696375269040281
1103	100	0.5993123796997928
1104	1	0.23247226992442194
1104	2	0.2973550928235511
1104	3	0.2825524492543323
1104	4	0.28334643770892853
1104	5	0.24054553644443644
1104	6	0.2932302247216114
1104	7	0.3273007599848583
1104	8	0.35911152665660495
1104	9	0.4216997493868817
1104	10-11	0.4497904379479962
1104	12-13	0.39811640774948387
1104	14-15	0.48318744028439653
1104	16-17	0.4807610504202273
1104	18-19	0.4216966471329542
1104	20-21	0.4264255341879917
1104	22-23	0.46008616459488394
1104	24-25	0.45237500721864166
1104	26-27	0.5253235879750875
1104	28-29	0.6005145819655411
1104	30-31	0.5647312935955568
1104	32-33	0.5278786923839434
1104	34-35	0.5229190627859666
1104	36-37	0.5194002267292959
1104	38-39	0.5346975753274563
1104	40-41	0.5379461955515694
1104	42-43	0.48065634645345057
1104	44-45	0.501284390020956
1104	46-47	0.5298671588571224
1104	48-49	0.49250672594990164
1104	50-51	0.4749560564460964
1104	52-53	0.4153646002768241
1104	54-55	0.4455967159450225
1104	56-57	0.4729819831597837
1104	58-59	0.4522478692494616
1104	60-61	0.4358316696255571
1104	62-63	0.46787490744236493
1104	64-65	0.5248140063711517
1104	66-67	0.518288940811054
1104	68-69	0.4954114334473516
1104	70-71	0.4301763277653876
1104	72-73	0.4800241675489403
1104	74-75	0.5197578890410597
1104	76-77	0.41613563854983937
1104	78-79	0.5090143973344503
1104	80-81	0.5006574570830722
1104	82-83	0.4277130693938105
1104	84-85	0.38635072725031705
1104	86-87	0.38060293021719716
1104	88-89	0.4069485445927583
1104	90-91	0.41694785758324926
1104	92-93	0.41600311131714207
1104	94-95	0.4258208937941532
1104	96-97	0.4731658327462256
1104	98-99	0.4692854092004559
1104	100	0.47454252259308305
1105	1	0.09988757665199799
1105	2	0.1470392534553362
1105	3	0.19423809707100403
1105	4	0.1421624055784747
1105	5	0.11554975492256148
1105	6	0.2335785056726678
1105	7	0.21082279849130003
1105	8	0.15346852396000799
1105	9	0.28013666076360977
1105	10-11	0.33029470881896117
1105	12-13	0.2544518862168559
1105	14-15	0.35490358402839917
1105	16-17	0.3574289353809519
1105	18-19	0.324196581566099
1105	20-21	0.27102377506501796
1105	22-23	0.2839266756975718
1105	24-25	0.28727999811437144
1105	26-27	0.3244531094103067
1105	28-29	0.41892090170311036
1105	30-31	0.3409325402707779
1105	32-33	0.3763482467144357
1105	34-35	0.3350226062081205
1105	36-37	0.30036553631853025
1105	38-39	0.2550006022890017
1105	40-41	0.22480566468298946
1105	42-43	0.3010192272813441
1105	44-45	0.22555288650391958
1105	46-47	0.22410761516750455
1105	48-49	0.20402165307874753
1105	50-51	0.21819857307212231
1105	52-53	0.21338209023878818
1105	54-55	0.2951581767876874
1105	56-57	0.3064575225736377
1105	58-59	0.21964395111969282
1105	60-61	0.19765028957436215
1105	62-63	0.23221799996252201
1105	64-65	0.29615323713084507
1105	66-67	0.28772848866686473
1105	68-69	0.25928010942700297
1105	70-71	0.30151988072136504
1105	72-73	0.28810214254162503
1105	74-75	0.3014659510179811
1105	76-77	0.2486932874652723
1105	78-79	0.2706887125780959
1105	80-81	0.239440265955551
1105	82-83	0.25557235872284423
1105	84-85	0.25432876864931586
1105	86-87	0.23148311583072
1105	88-89	0.23609912493768093
1105	90-91	0.19836811987930147
1105	92-93	0.20267842674345715
1105	94-95	0.24133032000325016
1105	96-97	0.3246893802486923
1105	98-99	0.34526943973449775
1105	100	0.3404061754115659
1106	1	0.12690266794946936
1106	2	0.18958834733211916
1106	3	0.18924194160604912
1106	4	0.18611002335644855
1106	5	0.14099974118887104
1106	6	0.1831847456425848
1106	7	0.2559226818270943
1106	8	0.2877428876798689
1106	9	0.3177607239023388
1106	10-11	0.3582910041067109
1106	12-13	0.31049323484347724
1106	14-15	0.3878790807343222
1106	16-17	0.33490566030680213
1106	18-19	0.375674983323826
1106	20-21	0.3830847419001202
1106	22-23	0.393107369270723
1106	24-25	0.34445830364388996
1106	26-27	0.3335791629523541
1106	28-29	0.4728761627406186
1106	30-31	0.42095139444924
1106	32-33	0.4081945725354572
1106	34-35	0.326620797347168
1106	36-37	0.37514301245690973
1106	38-39	0.3739097611036044
1106	40-41	0.37639413191121207
1106	42-43	0.3409816023777523
1106	44-45	0.3646254762924741
1106	46-47	0.34601731177665584
1106	48-49	0.3513925548041499
1106	50-51	0.3465660214159314
1106	52-53	0.3721783415531519
1106	54-55	0.41727256783951816
1106	56-57	0.36333784941143676
1106	58-59	0.385546016614434
1106	60-61	0.3392506658884997
1106	62-63	0.3282968849493457
1106	64-65	0.3540566396307767
1106	66-67	0.4142653349804917
1106	68-69	0.40692911493085404
1106	70-71	0.3649673068289516
1106	72-73	0.32927947380976974
1106	74-75	0.37350861127050905
1106	76-77	0.4177312804631441
1106	78-79	0.3538230807469098
1106	80-81	0.29329630335286794
1106	82-83	0.30186941556898006
1106	84-85	0.35259134018894045
1106	86-87	0.3722649629585213
1106	88-89	0.4603564276010985
1106	90-91	0.4359485807253911
1106	92-93	0.42312737522914645
1106	94-95	0.4790216672401648
1106	96-97	0.5379108206611889
1106	98-99	0.5134970920339832
1106	100	0.5127373640061954
1107	1	0.1613595490002453
1107	2	0.20181567171024284
1107	3	0.2658165105490262
1107	4	0.23347845519629118
1107	5	0.19126774159072113
1107	6	0.23762417709321682
1107	7	0.22719234027329804
1107	8	0.2978560746697241
1107	9	0.388482670046308
1107	10-11	0.36958315131651887
1107	12-13	0.30349124321710974
1107	14-15	0.36652721773528185
1107	16-17	0.3249956905121394
1107	18-19	0.3726015226308945
1107	20-21	0.42705288187654133
1107	22-23	0.4115699723507902
1107	24-25	0.42532460008639816
1107	26-27	0.42670926785838503
1107	28-29	0.47018418842189647
1107	30-31	0.40080836676031595
1107	32-33	0.43148758688712263
1107	34-35	0.3967505450953368
1107	36-37	0.373296103697335
1107	38-39	0.4295688344255524
1107	40-41	0.3960540429167949
1107	42-43	0.41838757018408046
1107	44-45	0.46185845296818684
1107	46-47	0.3985721026920217
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2216	1	-0.7282093655083308
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2216	3	-0.8200539775740765
2216	4	-0.6975739818899314
2216	5	-0.9093326647216671
2216	6	-0.8910996499411539
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2216	9	-1.2513440944787675
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2216	12-13	-1.3233609548337952
2216	14-15	-1.6235734264281163
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2216	66-67	-1.7710389262402373
2216	68-69	-1.7404126767527792
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2216	74-75	-1.7619274256226873
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2216	82-83	-1.6937429566960986
2216	84-85	-1.632087461760083
2216	86-87	-1.683475114367596
2216	88-89	-1.5884603246929387
2216	90-91	-1.7760862340588268
2216	92-93	-1.6634387299411735
2216	94-95	-1.613335125476393
2216	96-97	-1.7111284645461886
2216	98-99	-1.9538650266043724
2216	100	-1.9649346905669915
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	225598.0
3	44230.0
4	27765.0
5	23035.0
6	18740.0
7	19198.0
8	21840.0
9	22863.0
10	23487.0
11	24373.0
12	25889.0
13	27691.0
14	28833.0
15	29071.0
16	29339.0
17	29077.0
18	29131.0
19	28397.0
20	28456.0
21	30873.0
22	36640.0
23	50366.0
24	69408.0
25	87903.0
26	99008.0
27	94613.0
28	102508.0
29	125349.0
30	147963.0
31	173157.0
32	214574.0
33	279371.0
34	379498.0
35	549595.0
36	889574.0
37	1520726.0
38	2061290.0
39	902619.0
40	2705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44879233673034	21.64047966766969	14.555934791627168	22.354793203972797
2	15.024730981217314	21.59756906455864	40.22327919601376	23.154420758210286
3	17.827746271123864	27.31880493419189	30.519445857782586	24.334002936901662
4	12.226480897142775	18.66785217665006	39.46447218272934	29.64119474347783
5	12.452198976146532	39.71006851812485	35.35065503064568	12.487077475082932
6	28.59759921893313	39.76388964168956	18.505299208350902	13.133211931026409
7	25.397966726697934	34.39126567901912	23.010582842976287	17.20018475130665
8	23.90232783328506	37.33666177583177	20.97263678177667	17.788373609106504
9	24.440411224224285	18.51055687767283	22.063744118807076	34.98528777929581
10-11	22.729480341652533	28.8232724276344	29.194121781991445	19.25312544872162
12-13	23.179849586925684	26.756123820526106	28.819187271990863	21.244839320557343
14-15	21.063331263712946	28.72061385898758	26.147300465180667	24.068754412118807
16-17	20.410472263061223	31.85427824071752	27.065654152776276	20.669595343444982
18-19	20.396001843440477	30.817335449563267	28.750380457419354	20.0362822495769
20-21	21.803215157363155	29.744203014460048	28.240222531417462	20.212359296759335
22-23	21.498077748888303	29.810802721013417	28.12542287442163	20.56569665567665
24-25	21.134829648269207	29.918820075607606	28.129442093315856	20.816908182807335
26-27	20.9248042671687	29.92643807716975	28.21635740963395	20.932400246027598
28-29	20.89413254299567	29.85714521199203	28.55777044623562	20.69095179877668
30-31	21.106940094380764	30.064724958957044	28.14780520700789	20.680529739654297
32-33	20.788406132374863	29.943604163603123	28.382003705993668	20.885985998028342
34-35	21.227663682351704	29.620037713157117	28.3309065586967	20.821392045794482
36-37	21.20900068358345	29.74335881725868	28.096370837041896	20.951269662115973
38-39	20.970141256357522	29.86090218926502	28.329595078325625	20.83936147605183
40-41	21.013817062192764	29.53503577633746	28.475521010309436	20.97562615116034
42-43	20.92587486304672	29.62974824410335	28.501063746983128	20.943313145866806
44-45	20.981836435360112	29.67495673875369	28.34987980934199	20.993327016544207
46-47	21.116733642373774	29.586889615314455	28.263603713412405	21.032773028899367
48-49	20.900225017542194	29.5309144118624	28.341077628911155	21.227782941684247
50-51	20.923176636547332	29.52884798681436	28.272516914630607	21.275458462007702
52-53	20.926276647728862	29.562923079151954	28.31914097786687	21.191659295252315
54-55	20.959290166523246	29.7540444505811	28.20480304369868	21.081862339196977
56-57	20.983411208950777	29.49589013584506	28.17228618107521	21.348412474128953
58-59	21.1074336111479	29.209945297742006	28.33802431599889	21.344596775111206
60-61	21.038923956952317	29.512076109972785	28.240927322680882	21.208072610394012
62-63	20.806454082077053	29.455670857017367	28.357250082131248	21.38062497877433
64-65	21.021861572312023	29.310204256880628	28.25060614492764	21.417328025879705
66-67	20.79110141560923	29.267219014653957	28.24612171778214	21.695557851954675
68-69	21.06018355832493	29.42208038672852	28.21442772211325	21.3033083328333
70-71	20.990379058885857	29.212962626235843	28.27049470913933	21.526163605738965
72-73	20.86484006512522	29.15603505706668	28.430649489043407	21.54847538876469
74-75	20.898907181550157	29.17873064369919	28.338554058924743	21.58380811582591
76-77	20.86822534771773	29.101802272621963	28.11151132938355	21.91846105027676
78-79	20.976526344808168	29.07171723688918	28.370692537348454	21.581063880954197
80-81	20.723828224163732	29.140631635355025	28.361704009613803	21.773836130867444
82-83	20.739941527996795	29.12359740362956	28.519245934629723	21.61721513374393
84-85	20.772970661674005	28.841359303139242	28.45364223067236	21.93202780451439
86-87	21.034990199736278	28.942911770176266	28.24433247848514	21.777765551602315
88-89	20.853270762943797	28.911597583741898	28.35745337075607	21.87767828255824
90-91	20.78240654252887	28.793303327777796	28.29873075151042	22.12555937818291
92-93	20.847750063375937	28.816718202260795	28.332943673486245	22.00258806087703
94-95	20.850960049837038	28.584317345240386	28.48358515111031	22.08113745381226
96-97	20.968304347986745	28.755041205045593	28.22396203187155	22.052692415096104
98-99	20.745036673566112	28.73003256868166	28.315121629453486	22.20980912829874
100	20.832089860393182	28.550314669039118	28.258755457203883	22.358840013363814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20032.0
1	15282.0
2	9280.5
3	7025.5
4	5148.5
5	3865.0
6	3324.5
7	3190.5
8	3298.5
9	3408.0
10	3107.0
11	2724.0
12	2684.0
13	2777.5
14	2944.5
15	3249.0
16	3681.5
17	4346.5
18	5473.0
19	7241.0
20	9887.0
21	13703.0
22	19805.0
23	29247.0
24	41759.5
25	56893.5
26	76351.5
27	99275.5
28	127668.5
29	157889.5
30	178787.0
31	196951.5
32	218532.0
33	237260.5
34	252343.0
35	267823.5
36	279546.5
37	286934.0
38	293058.0
39	297582.5
40	302086.5
41	303296.5
42	302663.5
43	305748.5
44	306321.5
45	302181.5
46	297720.5
47	288739.0
48	276820.5
49	268431.0
50	260685.0
51	244075.5
52	221853.0
53	200823.5
54	187518.5
55	181317.5
56	162730.5
57	138670.0
58	122008.5
59	109526.0
60	94208.5
61	76586.0
62	64427.5
63	54270.0
64	44058.0
65	35256.0
66	28215.5
67	22371.0
68	17843.0
69	14070.5
70	10792.5
71	8186.5
72	6434.0
73	5158.0
74	3915.5
75	2823.0
76	2086.5
77	1570.0
78	1293.5
79	1259.5
80	936.5
81	471.5
82	362.5
83	283.5
84	202.5
85	174.5
86	124.0
87	98.0
88	104.5
89	79.5
90	48.0
91	44.0
92	44.0
93	40.5
94	37.0
95	37.0
96	43.5
97	39.0
98	32.5
99	45.0
100	63.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01390069600843567
2	0.007355051812058366
3	0.00943135830445762
4	0.008375609240525795
5	0.010287688100757874
6	0.01204726987397758
7	0.010111729923435906
8	0.007577932169999529
9	0.006416608199674524
10-11	0.006299302748126545
12-13	0.0065749705592642975
14-15	0.00757206689742213
16-17	0.004991346963366563
18-19	0.005566143675951667
20-21	0.005554413130796869
22-23	0.005894598940286011
24-25	0.00560720058399346
26-27	0.0059356558483278045
28-29	0.015337687789898429
30-31	0.00530807168254611
32-33	0.005319802227700909
34-35	0.0047743318780027995
36-37	0.006522183106067707
38-39	0.005859407304821618
40-41	0.005554413130796869
42-43	0.004745005515115804
44-45	0.004457607158823253
46-47	0.006076422390185381
48-49	0.0035954120899455975
50-51	0.003847618810773755
52-53	0.00316138191921807
54-55	0.004545586247484238
56-57	0.0030734028305570847
58-59	0.002838791927461124
60-61	0.003501567728707213
62-63	0.0033725317320044347
64-65	0.004563182065216435
66-67	0.003472241365820218
68-69	0.0037948313575771643
70-71	0.005055864961717953
72-73	0.0029560973790091045
74-75	0.002762543383954937
76-77	0.002791869746841932
78-79	0.0035543551819038044
80-81	0.0040001158977861296
82-83	0.0018827524973450843
84-85	0.002791869746841932
86-87	0.002786004474264533
88-89	0.0019590010408512717
90-91	0.0025865852066329666
92-93	0.0026276421146747596
94-95	0.0019883274037382666
96-97	0.0015191055975463453
98-99	0.0017889081361067
100	0.0028270613823063264
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8524753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.64164557520941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.09765203589049	33.123818548337226
2	16.667769077531435	16.214954319974385
3	6.517801555427946	9.511097795660243
4	3.1542989714418357	6.137211704284855
5	1.7243733551560885	4.193817879041858
6	1.0155368417503348	2.963842987499239
7	0.6311919255302506	2.1491549752103496
8	0.44329339763731873	1.7250016266963863
9	0.30541921347649925	1.3370483820404788
>10	1.288835510829091	10.782346732225852
>50	0.08550495270011252	2.9016891939925014
>100	0.06161051825338724	5.82871943840568
>500	0.005307500481472173	1.7495842254377603
>1k	0.001380917278666558	1.0783744754837234
>5k	0.0	0.0
>10k+	2.4226615039596456E-5	0.30333771570959345
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25741	0.30195596282965614	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.1730545154798033E-5	0.0	1.1730545154798033E-5	0.0
4	0.0	1.1730545154798033E-5	0.0	7.03832709287882E-5	0.0
5	0.0	2.3461090309596066E-5	0.0	7.03832709287882E-5	0.0
6	0.0	2.3461090309596066E-5	0.0	7.03832709287882E-5	0.0
7	0.0	2.3461090309596066E-5	0.0	8.211381608358624E-5	0.0
8	0.0	2.3461090309596066E-5	0.0	9.384436123838426E-5	0.0
9	0.0	2.3461090309596066E-5	0.0	1.2903599670277836E-4	0.0
10-11	0.0	2.3461090309596066E-5	0.0	1.4663181443497542E-4	0.0
12-13	0.0	3.51916354643941E-5	0.0	1.5249708701237444E-4	0.0
14-15	0.0	4.692218061919213E-5	0.0	1.8768872247676855E-4	0.0
16-17	0.0	5.865272577399017E-5	0.0	2.756678111377538E-4	0.0
18-19	1.1730545154798033E-5	8.797908866098526E-5	0.0	3.1085944660214787E-4	0.0
20-21	1.1730545154798033E-5	1.055749063931823E-4	0.0	3.5191635464394103E-4	0.0
22-23	1.1730545154798033E-5	1.1730545154798034E-4	0.0	6.275841657816948E-4	0.0
24-25	1.1730545154798033E-5	1.1730545154798034E-4	0.0	0.0011144017897058132	0.0
26-27	1.1730545154798033E-5	1.1730545154798034E-4	0.0	0.0019062135876546805	0.0
28-29	1.1730545154798033E-5	1.1730545154798034E-4	0.0	0.004398954433049262	0.0
30-31	1.1730545154798033E-5	1.1730545154798034E-4	0.0	0.011284784438915708	0.0
32-33	1.1730545154798033E-5	1.2317072412537934E-4	0.0	0.02641718768860517	0.0
34-35	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.051655455589153146	0.0
36-37	1.1730545154798033E-5	1.407665418575764E-4	0.0	0.09240736945692152	0.0
38-39	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.14592211645311015	0.0
40-41	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.19968907017012694	0.0
42-43	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.25489888094118385	0.0
44-45	1.1730545154798033E-5	1.5836235958977346E-4	0.0	0.3288013154164115	0.0
46-47	1.1730545154798033E-5	1.6422763216717248E-4	0.0	0.4231266290061425	0.0
48-49	1.1730545154798033E-5	1.700929047445715E-4	0.0	0.523516634440904	0.0
50-51	1.759581773219705E-5	2.580719934055567E-4	0.0	0.6220590790137849	0.0
52-53	2.3461090309596066E-5	2.580719934055567E-4	0.0	0.7190003041730358	0.0
54-55	2.3461090309596066E-5	2.6980253856035476E-4	0.0	0.8338364759659311	0.0
56-57	2.3461090309596066E-5	2.991289014473499E-4	0.0	0.9774652708412783	0.0
58-59	2.3461090309596066E-5	3.3432053691174394E-4	0.0	1.1389655512599601	0.0
60-61	2.3461090309596066E-5	3.5191635464394103E-4	0.0	1.3087651923756618	0.0
62-63	2.3461090309596066E-5	3.5191635464394103E-4	0.0	1.4799431725470522	0.0
64-65	2.3461090309596066E-5	3.5778162722134E-4	0.0	1.6658253910699816	0.0
66-67	2.3461090309596066E-5	3.69512172376138E-4	0.0	1.8883714284742328	0.0
68-69	2.3461090309596066E-5	3.7537744495353705E-4	0.0	2.1453524811803932	0.0
70-71	2.3461090309596066E-5	3.929732626857341E-4	0.0	2.4198941599832864	0.0
72-73	2.3461090309596066E-5	3.988385352631331E-4	0.0	2.703046058929801	0.0
74-75	2.3461090309596066E-5	4.222996255727292E-4	0.0	2.995940175627376	0.0
76-77	2.3461090309596066E-5	4.222996255727292E-4	0.0	3.3185829548375185	0.0
78-79	2.3461090309596066E-5	4.222996255727292E-4	0.0	3.688353199207062	0.0
80-81	2.3461090309596066E-5	4.222996255727292E-4	0.0	4.090317924753949	0.0
82-83	2.3461090309596066E-5	4.222996255727292E-4	0.0	4.509209826959209	0.0
84-85	2.3461090309596066E-5	4.222996255727292E-4	0.0	4.9291750740461335	0.0
86-87	2.3461090309596066E-5	4.222996255727292E-4	0.0	5.378959366916555	0.0
88	2.3461090309596066E-5	4.222996255727292E-4	0.0	5.744659112117383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9470	0.0	45.616295	1
GTATCAA	15865	0.0	33.77686	1
ATCAACG	17120	0.0	30.640408	3
TCAACGC	17190	0.0	30.242195	4
TATCAAC	20025	0.0	26.453644	2
CAACGCA	20230	0.0	25.697645	5
GTACATG	33690	0.0	24.430908	1
AACGCAG	21455	0.0	24.141073	6
TACATGG	32965	0.0	23.626724	2
CGCAGAG	21865	0.0	23.17236	8
ACGCAGA	22250	0.0	22.919266	7
ACATGGG	33750	0.0	21.879456	3
GTGGTAT	5065	0.0	21.438137	1
TGGTATC	5010	0.0	20.077522	2
CATGGGT	7475	0.0	20.059198	4
GCAGAGT	25370	0.0	18.970684	9
CAGAGTA	24655	0.0	17.290329	10-11
CATGGGG	16555	0.0	16.836802	4
CATGGGA	13960	0.0	16.43117	4
AGAGTAC	23385	0.0	16.068748	10-11
>>END_MODULE