FastQCFastQC Report
Wed 25 May 2016
SRR2431430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524753
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440290.5164841726206025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387050.45403075021645783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264190.30990927244460925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98000.11495934251702072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86790.10180940139849212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177650.059.1471181
GTATCAA334850.041.750571
GTGGTAT64050.035.557921
TGGTATC67550.033.490432
ATCAACG427850.032.4728163
TCAACGC434000.032.0234954
TATCAAC447950.031.0577262
CAACGCA458800.030.3079515
AACGCAG477250.029.1250616
ACGCAGA532600.025.9658727
CGCAGAG530900.025.9427688
GCAGAGT570500.023.9277789
GTACATG325850.023.610771
TACATGG323100.022.6793962
GAGTACT390350.021.80312212-13
ACATGGG327900.021.7162823
CAGAGTA566550.020.86290710-11
AGAGTAC546350.020.79969410-11
AGTACTT400600.020.36541212-13
CATGGGT73300.020.211924
Produced by FastQC (version 0.11.5)