##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431430_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8524753
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27000946537689	33.0	31.0	34.0	30.0	34.0
2	32.39055630116204	34.0	31.0	34.0	31.0	34.0
3	32.49911721782438	34.0	31.0	34.0	31.0	34.0
4	36.044529149407616	37.0	35.0	37.0	35.0	37.0
5	35.95660015017444	37.0	35.0	37.0	35.0	37.0
6	35.99480207813646	37.0	35.0	37.0	35.0	37.0
7	35.93158722604632	37.0	35.0	37.0	35.0	37.0
8	35.913466114502086	37.0	35.0	37.0	35.0	37.0
9	37.5986027982277	39.0	37.0	39.0	35.0	39.0
10-11	37.53734272418215	39.0	37.0	39.0	34.5	39.0
12-13	37.52258681277921	39.0	37.0	39.0	35.0	39.0
14-15	38.870295596834296	40.0	38.0	41.0	35.0	41.0
16-17	38.91650954578977	40.0	38.0	41.0	35.0	41.0
18-19	38.991321625389034	40.0	38.5	41.0	35.0	41.0
20-21	39.017956649301155	41.0	39.0	41.0	35.0	41.0
22-23	38.97572029359678	40.0	39.0	41.0	35.0	41.0
24-25	38.93124621909867	40.5	38.0	41.0	35.0	41.0
26-27	38.80047633051656	40.0	38.0	41.0	34.5	41.0
28-29	38.72277278884209	40.0	38.0	41.0	34.0	41.0
30-31	38.57205622262604	40.0	38.0	41.0	34.0	41.0
32-33	38.40738224321573	40.0	38.0	41.0	34.0	41.0
34-35	38.288146941031606	40.0	38.0	41.0	34.0	41.0
36-37	38.1650521721861	40.0	38.0	41.0	34.0	41.0
38-39	38.07240274292991	40.0	38.0	41.0	33.0	41.0
40-41	37.96581296842266	40.0	38.0	41.0	33.0	41.0
42-43	37.861304896458584	40.0	37.0	41.0	33.0	41.0
44-45	37.67921809582049	40.0	37.0	41.0	33.0	41.0
46-47	37.41387210866989	40.0	37.0	41.0	32.5	41.0
48-49	37.20492499899997	40.0	37.0	41.0	32.0	41.0
50-51	37.08717789242691	40.0	37.0	41.0	31.5	41.0
52-53	36.955567627589915	40.0	36.5	41.0	31.0	41.0
54-55	36.80898443626461	40.0	36.0	41.0	31.0	41.0
56-57	36.594630249111034	40.0	36.0	41.0	31.0	41.0
58-59	36.43246848325107	39.0	35.0	41.0	30.5	41.0
60-61	36.238926101436604	39.0	35.0	41.0	30.0	41.0
62-63	36.03063185525727	39.0	35.0	41.0	30.0	41.0
64-65	35.80196253193495	38.5	35.0	41.0	30.0	41.0
66-67	35.54630333570955	38.0	35.0	40.5	29.0	41.0
68-69	35.268707198906526	37.0	35.0	40.0	29.0	41.0
70-71	34.946644201890656	37.0	35.0	40.0	29.0	41.0
72-73	34.62935911456907	36.0	34.0	39.0	29.0	41.0
74-75	34.318086459513836	36.0	34.0	39.0	28.5	41.0
76-77	33.95966715985789	35.5	34.0	38.5	28.0	40.0
78-79	33.63955506980672	35.0	34.0	37.0	28.0	39.5
80-81	33.32749335963165	35.0	34.0	37.0	28.0	39.0
82-83	33.064075463535424	35.0	34.0	36.5	27.5	39.0
84-85	32.828075370629506	35.0	34.0	36.0	27.5	37.5
86-87	32.58131062565684	35.0	34.0	36.0	27.0	37.0
88-89	32.42507301971095	35.0	34.0	35.5	27.0	37.0
90-91	32.235279485517054	35.0	34.0	35.0	27.0	36.0
92-93	32.03872997845216	35.0	33.5	35.0	26.0	36.0
94-95	31.883709005997005	35.0	33.0	35.0	26.0	36.0
96-97	31.721869888781526	35.0	33.0	35.0	26.0	36.0
98-99	31.55864691915414	35.0	33.0	35.0	25.0	35.5
100	31.45369983153764	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15208270943352176
1101	2	0.22208757840306959
1101	3	0.1961107223772416
1101	4	0.18302099638063396
1101	5	0.16825167998575807
1101	6	0.17241791634249637
1101	7	0.1817778187350001
1101	8	0.17624310859000047
1101	9	0.296700490792297
1101	10-11	0.2965480399622109
1101	12-13	0.3029804444059252
1101	14-15	0.4066994460628095
1101	16-17	0.3683820130303559
1101	18-19	0.3560447852974704
1101	20-21	0.2913601788695459
1101	22-23	0.2741143675941373
1101	24-25	0.29367600053548415
1101	26-27	0.2633608050160774
1101	28-29	0.3288065319201863
1101	30-31	0.3626172598823132
1101	32-33	0.32864470088113507
1101	34-35	0.3744187083590802
1101	36-37	0.36427957534079525
1101	38-39	0.3241286110746415
1101	40-41	0.34919036920423707
1101	42-43	0.3317607138577401
1101	44-45	0.36413739576763504
1101	46-47	0.36949488637193895
1101	48-49	0.3840199371096773
1101	50-51	0.35385991425669516
1101	52-53	0.36107723832602545
1101	54-55	0.3738035837871081
1101	56-57	0.41104971550513625
1101	58-59	0.4150595531490069
1101	60-61	0.44227487766291773
1101	62-63	0.4420489594291652
1101	64-65	0.42346956960544446
1101	66-67	0.4130778144085099
1101	68-69	0.39004735732809337
1101	70-71	0.3951949898270968
1101	72-73	0.43679072057002344
1101	74-75	0.36944717039774844
1101	76-77	0.41060487564107007
1101	78-79	0.3407171278241776
1101	80-81	0.38303105027432593
1101	82-83	0.3855450798439506
1101	84-85	0.3826957696229698
1101	86-87	0.4315476321732703
1101	88-89	0.42159313630015305
1101	90-91	0.4728437744387577
1101	92-93	0.49776389441351654
1101	94-95	0.5096289174256228
1101	96-97	0.5242390635185323
1101	98-99	0.5488524958970835
1101	100	0.6078995840610624
1102	1	0.13506781892901643
1102	2	0.20377925642485906
1102	3	0.19802371421124576
1102	4	0.17482216153846508
1102	5	0.16606646594624408
1102	6	0.15483911311086018
1102	7	0.17297112123319636
1102	8	0.22376125757258336
1102	9	0.2961927270283624
1102	10-11	0.2641540152813988
1102	12-13	0.2766131168860184
1102	14-15	0.3984756282742197
1102	16-17	0.3717018622570123
1102	18-19	0.3441760893136845
1102	20-21	0.339158446005591
1102	22-23	0.30324306268889245
1102	24-25	0.3007652666919327
1102	26-27	0.3232117562563843
1102	28-29	0.3185969041109402
1102	30-31	0.3172404167842444
1102	32-33	0.32018784818426127
1102	34-35	0.3245814066643504
1102	36-37	0.33872527979471556
1102	38-39	0.36960120820817366
1102	40-41	0.37178623269674915
1102	42-43	0.41234065911918805
1102	44-45	0.41215177792349067
1102	46-47	0.43358069769605123
1102	48-49	0.42421824990017143
1102	50-51	0.4101221780425206
1102	52-53	0.3711260645286032
1102	54-55	0.38636954628561426
1102	56-57	0.45713080196083666
1102	58-59	0.42911296184226444
1102	60-61	0.3684423332559419
1102	62-63	0.39382665064395184
1102	64-65	0.37020814203810914
1102	66-67	0.3761637146351404
1102	68-69	0.41078366241215036
1102	70-71	0.3478580298723415
1102	72-73	0.3525954755850762
1102	74-75	0.4142268876543014
1102	76-77	0.3650511048204166
1102	78-79	0.351127204089849
1102	80-81	0.32013159468531427
1102	82-83	0.29922937594817967
1102	84-85	0.2872412788589713
1102	86-87	0.3175165243107827
1102	88-89	0.3031244639123045
1102	90-91	0.26861833935949164
1102	92-93	0.27094618870694376
1102	94-95	0.32233536095363746
1102	96-97	0.3661996599809463
1102	98-99	0.43217373882122345
1102	100	0.42021824881198455
1103	1	0.1787715446088356
1103	2	0.22209516643778215
1103	3	0.19765371956560074
1103	4	0.1782249165437051
1103	5	0.1352463425040611
1103	6	0.12968617031323504
1103	7	0.14091618868171452
1103	8	0.1273354977230028
1103	9	0.24575890785200016
1103	10-11	0.2441610184761771
1103	12-13	0.23594675464286752
1103	14-15	0.3472924319107804
1103	16-17	0.35652391594582866
1103	18-19	0.32255418605181063
1103	20-21	0.30703233026702037
1103	22-23	0.2788368649580235
1103	24-25	0.32350795498444995
1103	26-27	0.3161217234404887
1103	28-29	0.34372700073093654
1103	30-31	0.3071263597414102
1103	32-33	0.3369665654660494
1103	34-35	0.3552863184402497
1103	36-37	0.3683448680942405
1103	38-39	0.38455120349980376
1103	40-41	0.47232538556788484
1103	42-43	0.5127256073416717
1103	44-45	0.4855948251758093
1103	46-47	0.4940367003824164
1103	48-49	0.46078107703904436
1103	50-51	0.45439646280237156
1103	52-53	0.4681475014063423
1103	54-55	0.48499091122876337
1103	56-57	0.508599595912159
1103	58-59	0.4835673291296132
1103	60-61	0.5188488166094629
1103	62-63	0.5067600287834964
1103	64-65	0.457942371134628
1103	66-67	0.5083069303820196
1103	68-69	0.4441174137512576
1103	70-71	0.5094493857485816
1103	72-73	0.5015481135505837
1103	74-75	0.45162501817455336
1103	76-77	0.4598700836913423
1103	78-79	0.4823598848165247
1103	80-81	0.46198331413733484
1103	82-83	0.4296511881437155
1103	84-85	0.43591881324960013
1103	86-87	0.4285717968695337
1103	88-89	0.44199534784485905
1103	90-91	0.4397288523526015
1103	92-93	0.4669631840320605
1103	94-95	0.5080718552278256
1103	96-97	0.49482927398915066
1103	98-99	0.5392890280849123
1103	100	0.5428593316201535
1104	1	0.10316056646233562
1104	2	0.14669406482415326
1104	3	0.14216353852798136
1104	4	0.1352813912156634
1104	5	0.11828810693709357
1104	6	0.13664838209950148
1104	7	0.12027423900688206
1104	8	0.12481897334633629
1104	9	0.21495516012252835
1104	10-11	0.2226230162624674
1104	12-13	0.20547010584736114
1104	14-15	0.3129396216675744
1104	16-17	0.27944936562516887
1104	18-19	0.26390756092209244
1104	20-21	0.2719955226669555
1104	22-23	0.23702549249819072
1104	24-25	0.236944987667286
1104	26-27	0.2565256011700683
1104	28-29	0.271744285763873
1104	30-31	0.3069883970929652
1104	32-33	0.2839388487656649
1104	34-35	0.3097654142649233
1104	36-37	0.2699316127202849
1104	38-39	0.3180775536579077
1104	40-41	0.28273679649995387
1104	42-43	0.297495659665195
1104	44-45	0.27651577019650375
1104	46-47	0.39038709875026
1104	48-49	0.32681135701244557
1104	50-51	0.3042086263210919
1104	52-53	0.28895336427177654
1104	54-55	0.3077779292961864
1104	56-57	0.30839324952939506
1104	58-59	0.36654091415361734
1104	60-61	0.3553695587523862
1104	62-63	0.29989701076905106
1104	64-65	0.2990117449357257
1104	66-67	0.2839079409010239
1104	68-69	0.2950393897530361
1104	70-71	0.3226801471320542
1104	72-73	0.29345396183752825
1104	74-75	0.3189330020103114
1104	76-77	0.2906385890016594
1104	78-79	0.3109396478198647
1104	80-81	0.30148001098523025
1104	82-83	0.2882955090750272
1104	84-85	0.2770659045117583
1104	86-87	0.27752455715890534
1104	88-89	0.22849068103072057
1104	90-91	0.2668093601093062
1104	92-93	0.29156100417174713
1104	94-95	0.29052368332615686
1104	96-97	0.28789904403359756
1104	98-99	0.3270427096203754
1104	100	0.3602413058268219
1105	1	0.09804023168972975
1105	2	0.15394096747861852
1105	3	0.1225083236313651
1105	4	0.10782209488959893
1105	5	0.07809290670270741
1105	6	0.12814165566788205
1105	7	0.14467214541895146
1105	8	0.17082571403161495
1105	9	0.17526811212290738
1105	10-11	0.16881397737214598
1105	12-13	0.12322922491121346
1105	14-15	0.20176007780376182
1105	16-17	0.20079165273443778
1105	18-19	0.20165098773252765
1105	20-21	0.1891399327400478
1105	22-23	0.20137504890352886
1105	24-25	0.19440487464094502
1105	26-27	0.20194614261959032
1105	28-29	0.18037098801836038
1105	30-31	0.20321056887540578
1105	32-33	0.16481002108965725
1105	34-35	0.21161543922157477
1105	36-37	0.20390129089388864
1105	38-39	0.21960732000383842
1105	40-41	0.24319496907924787
1105	42-43	0.21621641584648188
1105	44-45	0.18939358540743712
1105	46-47	0.2126132974415853
1105	48-49	0.17412290422018373
1105	50-51	0.13630789558484224
1105	52-53	0.16399908820762477
1105	54-55	0.16154412713011368
1105	56-57	0.2001211226441768
1105	58-59	0.17464975287883533
1105	60-61	0.19290822651780104
1105	62-63	0.18101256368591834
1105	64-65	0.16677228451371207
1105	66-67	0.1950050882733123
1105	68-69	0.16991484911830668
1105	70-71	0.17477431127908716
1105	72-73	0.18645671909063566
1105	74-75	0.17828114105552118
1105	76-77	0.21288954418520234
1105	78-79	0.12704445565730538
1105	80-81	0.14718890042679078
1105	82-83	0.13855821684766312
1105	84-85	0.1527619018474482
1105	86-87	0.16009620681202819
1105	88-89	0.1453259321551741
1105	90-91	0.185509897315967
1105	92-93	0.18454429759118085
1105	94-95	0.21319186449623118
1105	96-97	0.2791597129620662
1105	98-99	0.3163939273357954
1105	100	0.3298875208990637
1106	1	0.07319047017212199
1106	2	0.1257306765491819
1106	3	0.08101075945054959
1106	4	0.12252944194072057
1106	5	0.07567880314226727
1106	6	0.06997473892532469
1106	7	0.10066600969348372
1106	8	0.12696625492944946
1106	9	0.13674926357902706
1106	10-11	0.13373803141031004
1106	12-13	0.14033325981927902
1106	14-15	0.20378275861919093
1106	16-17	0.18346264600045004
1106	18-19	0.18218973661632987
1106	20-21	0.11928535365709081
1106	22-23	0.14248438464865387
1106	24-25	0.14900179530071966
1106	26-27	0.18604767982280634
1106	28-29	0.14577320299517993
1106	30-31	0.19711863569431642
1106	32-33	0.2187350998400035
1106	34-35	0.20726294566689774
1106	36-37	0.234275935021671
1106	38-39	0.21863203468932824
1106	40-41	0.26357017915080405
1106	42-43	0.33372466553353064
1106	44-45	0.25978136319515954
1106	46-47	0.25378146955895176
1106	48-49	0.2899671951531104
1106	50-51	0.304516504544992
1106	52-53	0.2766035048939628
1106	54-55	0.3279054937030992
1106	56-57	0.36709239396011384
1106	58-59	0.349298700829479
1106	60-61	0.3283758307879481
1106	62-63	0.26626930388574266
1106	64-65	0.2550127919402527
1106	66-67	0.2852900483641605
1106	68-69	0.28130224697439843
1106	70-71	0.33315985976206974
1106	72-73	0.3440010284282806
1106	74-75	0.35179390153111
1106	76-77	0.39061625681204504
1106	78-79	0.3016599601304222
1106	80-81	0.26313294176207336
1106	82-83	0.24112935057095797
1106	84-85	0.2452770485674023
1106	86-87	0.27241872880807705
1106	88-89	0.2500218852819174
1106	90-91	0.30534793902690893
1106	92-93	0.34833250211068645
1106	94-95	0.3402556297996071
1106	96-97	0.33909558872650436
1106	98-99	0.36006713754836284
1106	100	0.4051281422956272
1107	1	0.08232816020142764
1107	2	0.1410766882223342
1107	3	0.09691651220254727
1107	4	0.11457886409394291
1107	5	0.07975871567356307
1107	6	0.059240705875694744
1107	7	0.019284017666009845
1107	8	0.007363103121335257
1107	9	0.054815196096619445
1107	10-11	0.07510932780812851
1107	12-13	0.019085267182170185
1107	14-15	0.13331195139934948
1107	16-17	0.1213100684031474
1107	18-19	0.1023397866883684
1107	20-21	0.0962169206639345
1107	22-23	0.09458818270823599
1107	24-25	0.10901071591000289
1107	26-27	0.14874031567792656
1107	28-29	0.12179091646878959
1107	30-31	0.14479350953459402
1107	32-33	0.14207839755805907
1107	34-35	0.16102222900377683
1107	36-37	0.15692798336669966
1107	38-39	0.10471639948909939
1107	40-41	0.1672885735634111
1107	42-43	0.16207504515329418
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2216	76-77	-0.8409347557958569
2216	78-79	-0.7760218121840694
2216	80-81	-0.8537539496335356
2216	82-83	-0.8958459746071981
2216	84-85	-0.8842160688940446
2216	86-87	-0.9320266360225204
2216	88-89	-0.8861962385774689
2216	90-91	-0.8784423811931568
2216	92-93	-0.8172482435826467
2216	94-95	-0.9175407332130305
2216	96-97	-1.2023973457443553
2216	98-99	-1.2461702241446595
2216	100	-1.2876073528114098
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	274.0
3	787.0
4	600.0
5	461.0
6	669.0
7	1550.0
8	3372.0
9	5565.0
10	7650.0
11	9662.0
12	13071.0
13	18068.0
14	22061.0
15	30162.0
16	39055.0
17	55179.0
18	61535.0
19	36182.0
20	24767.0
21	23049.0
22	23375.0
23	25228.0
24	29256.0
25	36552.0
26	46433.0
27	60954.0
28	78276.0
29	100296.0
30	128403.0
31	162123.0
32	205769.0
33	262068.0
34	349075.0
35	514828.0
36	826200.0
37	1578310.0
38	2450403.0
39	1285526.0
40	7959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.47103844902137	22.011900722702315	15.113478898756542	24.40358192951977
2	13.673745151325791	21.814778680391093	39.69513251586292	24.81634365242019
3	16.02943149964386	27.167455116540744	31.702171936189522	25.100941447625875
4	11.17430596461803	18.797913133795106	39.076856111927476	30.95092478965939
5	11.762687455920672	38.85412986057442	35.89136554297521	13.491817140529708
6	28.433832628347123	39.8130362252138	17.96201016029438	13.791120986144701
7	24.970471285209083	34.30407895689177	23.04989951028493	17.675550247614215
8	24.642250631777834	36.15670741427933	20.463701411642074	18.737340542300757
9	24.85015108355632	16.63801872030779	22.299473075642194	36.2123571204937
10-11	23.022041712643485	27.768174893962712	29.186860439285645	20.02292295410816
12-13	24.034303398585273	25.687471531433225	29.361877112451236	20.916347957530267
14-15	21.438767785999197	27.394359695817577	27.518820779909987	23.64805173827324
16-17	19.783335657936366	30.676777379942855	28.39633007548723	21.14355688663355
18-19	19.6546339817705	28.64984475210015	31.793994500485823	19.90152676564353
20-21	20.903579259129266	27.67790456802678	31.674542359174513	19.74397381366944
22-23	20.554448908959593	27.74111461059341	31.645292244831026	20.059144235615975
24-25	20.37367297382899	27.78906002557275	31.59964573533379	20.237621265264465
26-27	20.13362146680379	27.872426333056215	31.643262860519243	20.350689339620747
28-29	20.1799308997589	27.7596781218661	31.965166702894653	20.095224275480348
30-31	20.40947593872085	27.94166649803374	31.61175245390236	20.037105109343056
32-33	20.062905125873304	27.90111842087899	31.777751644697773	20.258224808549937
34-35	20.48471771908419	27.702935653016898	31.584696357405633	20.22765027049328
36-37	20.519984252286257	27.796773548721017	31.235122140975825	20.448120058016904
38-39	20.316074659649512	27.92757669349109	31.444150987640935	20.312197659218466
40-41	20.38773057893887	27.690904612989392	31.431976174054636	20.4893886340171
42-43	20.37361533768783	27.80546856382	31.33006354025085	20.490852558241322
44-45	20.505058651262896	27.88673923377834	31.010261001712365	20.597941113246396
46-47	20.702775441922423	27.89874663837716	30.625547635642224	20.772930284058194
48-49	20.659860647442628	28.0052081543194	30.27691033207116	21.05802086616681
50-51	20.739819617734213	28.065684112432717	30.01650455090747	21.177991718925604
52-53	20.753424396406785	28.241924694511905	29.929091159167022	21.075559749914294
54-55	20.814636834383084	28.502734567341854	29.788410854043107	20.894217744231952
56-57	20.8213485996507	28.14220081913725	29.83748938684952	21.19896119436253
58-59	20.851258998956414	27.811081555910334	30.06610941879284	21.27155002634041
60-61	20.793075096429146	28.129602460064945	29.918566773333765	21.158755670172148
62-63	20.565126393103764	28.003302936564694	30.130421769021904	21.301148901309638
64-65	20.637790183365823	27.98915686069568	30.04093539056663	21.33211756537187
66-67	20.56558290013367	27.941956035048555	29.981401203203145	21.51105986161463
68-69	20.889488376523385	28.070708050629893	29.816478892421504	21.22332468042522
70-71	20.837768229468637	27.938452644869706	29.811434873436614	21.412344252225044
72-73	20.696781478595334	27.909969942824148	29.88727063411691	21.505977944463613
74-75	20.7141164588204	28.02434798521478	29.674716970508157	21.586818585456665
76-77	20.79463768627666	27.953695549888657	29.47774557221775	21.773921191616928
78-79	20.82374844305408	27.99718394848289	29.66091952539807	21.518148083064954
80-81	20.55180953629976	28.055340723654986	29.643679998704947	21.749169741340307
82-83	20.57434531865631	27.975793763096192	29.762691484853505	21.68716943339399
84-85	20.570761501373834	27.835393366968148	29.62372630400205	21.97011882765597
86-87	20.680335254288305	27.927554030011194	29.494878033416335	21.89723268228417
88-89	20.46508561597034	27.929260824331216	29.57038755257777	22.035266007120676
90-91	20.38967580644272	27.8823972964378	29.432612299734668	22.295314597384817
92-93	20.462833372689417	27.894076657668144	29.47558697160063	22.167502998041815
94-95	20.37484253209448	27.72590009352764	29.560023615933506	22.33923375844438
96-97	20.442902965103276	27.951063211933917	29.264110580164505	22.341923242798302
98-99	20.22916908610381	27.90642731541601	29.362805303640826	22.501598294839358
100	20.254767192462772	27.866098656097254	29.29947057523505	22.57966357620493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2550.0
1	1582.5
2	1947.0
3	3500.5
4	2407.0
5	1571.5
6	4710.5
7	12408.5
8	20464.0
9	22334.0
10	15307.0
11	7839.0
12	6467.5
13	6995.0
14	7688.0
15	8487.0
16	9295.0
17	10219.5
18	11525.0
19	13118.0
20	15418.0
21	19022.0
22	24410.0
23	33125.0
24	44878.5
25	59197.5
26	78143.5
27	100219.5
28	127614.5
29	157342.0
30	178057.0
31	196249.5
32	217324.5
33	235656.5
34	251752.0
35	267785.5
36	278345.0
37	285563.5
38	291765.0
39	295594.5
40	300649.5
41	303183.0
42	303237.5
43	306892.0
44	308362.0
45	304089.0
46	298029.0
47	288421.5
48	273055.0
49	263531.5
50	261271.5
51	246051.5
52	219726.0
53	196582.0
54	183281.0
55	174630.5
56	154862.0
57	131360.5
58	116646.0
59	107375.0
60	92003.0
61	72601.0
62	60670.0
63	50971.0
64	40815.0
65	32121.0
66	25452.5
67	20115.5
68	15645.0
69	12018.5
70	9072.0
71	6661.0
72	5081.0
73	3952.0
74	2920.0
75	1996.5
76	1339.5
77	989.5
78	814.0
79	745.0
80	501.5
81	188.5
82	95.5
83	56.5
84	31.0
85	16.0
86	10.5
87	7.0
88	5.0
89	4.0
90	1.5
91	0.0
92	0.0
93	0.0
94	2.0
95	3.5
96	4.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04802485186374315
2	0.0
3	1.055749063931823E-4
4	0.0698084742162031
5	0.004093960259024513
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011525260614589068
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.217188932042957E-4
26-27	0.0
28-29	4.222996255727292E-4
30-31	0.0037596397221127697
32-33	1.2317072412537934E-4
34-35	0.0016657374119813206
36-37	0.0040001158977861296
38-39	0.001425261236307961
40-41	0.0011319976074380103
42-43	0.0018358303167258921
44-45	5.865272577399017E-6
46-47	4.516259884597243E-4
48-49	5.161439868111134E-4
50-51	0.0015777583233203356
52-53	8.856561591872516E-4
54-55	0.001120267062283212
56-57	0.006340359656168337
58-59	0.00913222940301027
60-61	0.0011730545154798034
62-63	0.0025513935711685724
64-65	0.0011730545154798034
66-67	9.384436123838428E-5
68-69	7.742159802166703E-4
70-71	3.812427175309361E-4
72-73	0.0
74-75	2.6980253856035476E-4
76-77	0.0
78-79	5.982578028946997E-4
80-81	0.0
82-83	0.0010616143365092222
84-85	6.334494383590938E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.580719934055567E-4
94-95	0.0
96-97	4.8681762392411844E-4
98-99	5.044134416563155E-4
100	3.4018580948914294E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8524753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.24607549524313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.10773756428163	27.291619830389145
2	18.729859377970712	16.199858253500178
3	7.628617409521138	9.897232932494319
4	3.769298348072763	6.520294436993998
5	2.036666263352729	4.403891149178342
6	1.23405284892417	3.202076560181672
7	0.7877471838142824	2.3846881927678343
8	0.5370708978205251	1.8580966874755553
9	0.3752807952980038	1.4606479444835123
>10	1.6020842551596348	11.84008017667269
>50	0.10408854652860008	3.1288844674428447
>100	0.07818247649013066	6.616584080652456
>500	0.006897775895036592	2.0233489403374763
>1k	0.002280512116402932	1.6741874437949875
>5k	5.429790165018603E-5	0.21695927377357424
>10k+	8.144685247527904E-5	1.2815496298612994
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44029	0.5164841726206025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38705	0.45403075021645783	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26419	0.30990927244460925	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9800	0.11495934251702072	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8679	0.10180940139849212	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3461090309596066E-5	0.0
3	0.0	1.1730545154798033E-5	0.0	3.51916354643941E-5	0.0
4	0.0	1.1730545154798033E-5	0.0	7.03832709287882E-5	0.0
5	0.0	2.3461090309596066E-5	0.0	7.03832709287882E-5	0.0
6	0.0	2.3461090309596066E-5	0.0	7.03832709287882E-5	0.0
7	0.0	2.3461090309596066E-5	0.0	7.03832709287882E-5	0.0
8	0.0	2.3461090309596066E-5	0.0	7.03832709287882E-5	0.0
9	0.0	2.3461090309596066E-5	0.0	1.2903599670277836E-4	0.0
10-11	0.0	2.932636288699508E-5	0.0	1.7595817732197051E-4	0.0
12-13	0.0	3.51916354643941E-5	0.0	2.2288035794116264E-4	0.0
14-15	0.0	3.51916354643941E-5	0.0	2.6393726598295577E-4	0.0
16-17	0.0	3.51916354643941E-5	0.0	3.5191635464394103E-4	0.0
18-19	5.865272577399016E-6	3.51916354643941E-5	0.0	3.7537744495353705E-4	0.0
20-21	1.1730545154798033E-5	4.692218061919214E-5	0.0	4.0470380784053217E-4	0.0
22-23	1.1730545154798033E-5	8.211381608358624E-5	0.0	6.803716189782859E-4	0.0
24-25	1.1730545154798033E-5	8.211381608358624E-5	0.0	0.0011847850606346013	0.0
26-27	1.1730545154798033E-5	1.114401789705813E-4	0.0	0.0020352495843574585	0.0
28-29	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.004598373700680829	0.0
30-31	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.01170121879191104	0.0
32-33	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.02709169403500606	0.0
34-35	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.052963411373913125	0.0
36-37	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.09459511612829134	0.0
38-39	1.1730545154798033E-5	1.2903599670277836E-4	0.0	0.14948820218016873	0.0
40-41	1.1730545154798033E-5	1.407665418575764E-4	0.0	0.20452205477390373	0.0
42-43	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.261309623868281	0.0
44-45	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.3371241372037407	0.0
46-47	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.43347883510525176	0.0
48-49	1.1730545154798033E-5	1.5249708701237444E-4	0.0	0.5358102457631324	0.0
50-51	1.1730545154798033E-5	2.3461090309596068E-4	0.0	0.6361885206527391	0.0
52-53	1.1730545154798033E-5	2.5220672082815773E-4	0.0	0.7352823008478955	0.0
54-55	1.1730545154798033E-5	2.639372659829557E-4	0.0	0.8527167883925786	0.0
56-57	1.1730545154798033E-5	2.815330837151528E-4	0.0	0.9997005191821979	0.0
58-59	1.1730545154798033E-5	2.815330837151528E-4	0.0	1.1649076518697963	0.0
60-61	1.1730545154798033E-5	2.815330837151528E-4	0.0	1.3383906841641042	0.0
62-63	1.1730545154798033E-5	2.9326362886995084E-4	0.0	1.513809256408954	0.0
64-65	1.1730545154798033E-5	3.3432053691174394E-4	0.0	1.7040845640923554	0.0
66-67	1.1730545154798033E-5	3.5191635464394103E-4	0.0	1.9309239810232626	0.0
68-69	1.1730545154798033E-5	3.5191635464394103E-4	0.0	2.1927204225154675	0.0
70-71	1.1730545154798033E-5	3.5191635464394103E-4	0.0	2.47211268173987	0.0
72-73	1.1730545154798033E-5	3.63646899798739E-4	0.0	2.760701688365634	0.0
74-75	1.1730545154798033E-5	3.63646899798739E-4	0.0	3.058810032384516	0.0
76-77	1.1730545154798033E-5	3.63646899798739E-4	0.0	3.387499907621957	0.0
78-79	1.1730545154798033E-5	3.63646899798739E-4	0.0	3.7641618472699445	0.0
80-81	1.1730545154798033E-5	3.63646899798739E-4	0.0	4.174115074067249	5.865272577399016E-6
82-83	1.1730545154798033E-5	3.63646899798739E-4	0.0	4.600737405529521	1.1730545154798033E-5
84-85	1.1730545154798033E-5	3.871079901083351E-4	0.0	5.029588540571205	1.1730545154798033E-5
86-87	1.1730545154798033E-5	3.871079901083351E-4	0.0	5.490153204438885	1.1730545154798033E-5
88	1.1730545154798033E-5	3.871079901083351E-4	0.0	5.8649910443153015	1.1730545154798033E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17765	0.0	59.147118	1
GTATCAA	33485	0.0	41.75057	1
GTGGTAT	6405	0.0	35.55792	1
TGGTATC	6755	0.0	33.49043	2
ATCAACG	42785	0.0	32.472816	3
TCAACGC	43400	0.0	32.023495	4
TATCAAC	44795	0.0	31.057726	2
CAACGCA	45880	0.0	30.307951	5
AACGCAG	47725	0.0	29.125061	6
ACGCAGA	53260	0.0	25.965872	7
CGCAGAG	53090	0.0	25.942768	8
GCAGAGT	57050	0.0	23.927778	9
GTACATG	32585	0.0	23.61077	1
TACATGG	32310	0.0	22.679396	2
GAGTACT	39035	0.0	21.803122	12-13
ACATGGG	32790	0.0	21.716282	3
CAGAGTA	56655	0.0	20.862907	10-11
AGAGTAC	54635	0.0	20.799694	10-11
AGTACTT	40060	0.0	20.365412	12-13
CATGGGT	7330	0.0	20.21192	4
>>END_MODULE