FastQCFastQC Report
Wed 25 May 2016
SRR2431429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441898
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA371540.8364442407277249No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54400.12247016928349098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.045.43251
TCTGTCG17050.036.9384778
GTATCAA88350.033.464681
GTCTCGC18050.031.2497561
ATAACGC3450.028.6106783
CGCTCTG16950.028.2848245
TACCTGG37250.027.7603112
ATCAACG102250.027.7192763
GTACCTG39900.027.4489421
CTGTCGC22350.027.3378549
TCAACGC103650.027.2995264
TCTCGCT20050.026.9594732
TCGCTCT19300.026.789424
TATCAAC111550.025.7453942
GTACATG179350.025.395981
CAACGCA113350.025.1289375
CTCGCTC20100.025.0216333
CATGGGT74950.024.269864
TACATGG177650.024.2358472
AACGCAG119000.024.0927496
Produced by FastQC (version 0.11.5)