##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431429_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4441898 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.51233999520025 31.0 30.0 34.0 23.0 34.0 2 29.476121919053522 31.0 30.0 34.0 23.0 34.0 3 29.62869971350085 31.0 30.0 34.0 23.0 34.0 4 32.865285740465 37.0 35.0 37.0 23.0 37.0 5 32.98227874660787 37.0 35.0 37.0 25.0 37.0 6 32.97852652177065 37.0 35.0 37.0 25.0 37.0 7 32.76941996416847 37.0 35.0 37.0 23.0 37.0 8 32.71311678025925 37.0 35.0 37.0 22.0 37.0 9 33.94914966530074 39.0 35.0 39.0 18.0 39.0 10-11 33.935209565820735 39.0 35.0 39.0 18.0 39.0 12-13 33.90156719042175 39.0 35.0 39.0 17.5 39.0 14-15 35.02129168657182 40.0 35.5 41.0 17.5 41.0 16-17 35.00866026189705 40.0 35.5 41.0 18.0 41.0 18-19 34.988536206819695 40.0 35.0 41.0 18.0 41.0 20-21 35.101521916982335 40.0 35.0 41.0 20.0 41.0 22-23 35.05261917315526 40.0 35.0 41.0 21.5 41.0 24-25 35.101510435403966 40.0 35.0 41.0 23.0 41.0 26-27 35.109549453859586 40.0 35.0 41.0 24.0 41.0 28-29 35.01326808945185 40.0 34.0 41.0 24.0 41.0 30-31 34.98429792849814 40.0 34.0 41.0 24.0 41.0 32-33 34.92779291645148 40.0 34.0 41.0 24.0 41.0 34-35 34.82904537654849 40.0 34.0 41.0 23.5 41.0 36-37 34.69081100016254 40.0 34.0 41.0 23.0 41.0 38-39 34.564620574358074 39.0 34.0 41.0 22.0 41.0 40-41 34.46843004949686 39.0 34.0 41.0 21.5 41.0 42-43 34.22722842802784 39.0 33.0 41.0 19.0 41.0 44-45 34.06915354652448 39.0 33.0 41.0 18.0 41.0 46-47 33.921448669014914 39.0 33.0 41.0 17.5 41.0 48-49 33.78175016625776 38.5 33.0 40.0 15.5 41.0 50-51 33.60561341120395 38.0 33.0 40.0 15.0 41.0 52-53 33.498422521183514 38.0 33.0 40.0 13.5 41.0 54-55 33.420653851124 38.0 32.5 40.0 12.5 41.0 56-57 33.26909938049005 38.0 32.0 40.0 8.5 41.0 58-59 33.05600792724191 38.0 32.0 40.0 8.0 41.0 60-61 32.81969847123909 37.0 32.0 40.0 6.5 41.0 62-63 32.51139659217749 37.0 31.0 40.0 2.0 41.0 64-65 32.349334563738296 36.5 31.0 40.0 2.0 41.0 66-67 32.09084663808129 36.0 31.0 39.5 2.0 41.0 68-69 31.8130192318689 36.0 31.0 39.0 2.0 41.0 70-71 31.499055021074327 35.0 31.0 39.0 2.0 41.0 72-73 31.181977163816008 35.0 31.0 38.5 2.0 40.0 74-75 30.860662153881066 35.0 30.0 37.5 2.0 40.0 76-77 30.55188705368741 35.0 30.0 37.0 2.0 39.5 78-79 30.22220467466835 35.0 30.0 36.5 2.0 39.0 80-81 29.927373951405457 35.0 30.0 36.0 2.0 39.0 82-83 29.63390750980774 35.0 30.0 36.0 2.0 37.5 84-85 29.345045293701027 34.0 29.0 35.0 2.0 37.0 86-87 29.077657568904105 34.0 29.0 35.0 2.0 37.0 88-89 28.813452717734627 34.0 29.0 35.0 2.0 36.0 90-91 28.59765273763603 34.0 29.0 35.0 2.0 36.0 92-93 28.388759264620667 34.0 29.0 35.0 2.0 36.0 94-95 28.138823313817653 34.0 28.5 35.0 2.0 35.5 96-97 28.032060056309263 34.0 29.0 35.0 2.0 35.0 98-99 27.864241592220264 34.0 28.0 35.0 2.0 35.0 100 27.715472755115044 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7182656837246455 1101 2 0.8410096602541195 1101 3 0.871674965676366 1101 4 0.8718233887513307 1101 5 0.7278934800206542 1101 6 0.6791775397482382 1101 7 0.726082545999418 1101 8 0.9051457106699772 1101 9 0.9197150704419812 1101 10-11 1.081803370669192 1101 12-13 0.9951297775408676 1101 14-15 1.0972948741757875 1101 16-17 1.022213016181162 1101 18-19 1.0235082483216829 1101 20-21 1.0324167137148237 1101 22-23 0.964712300960791 1101 24-25 0.9872047262994812 1101 26-27 1.0499975436158024 1101 28-29 1.105535917556736 1101 30-31 1.0872967618186564 1101 32-33 1.099802511757666 1101 34-35 1.0924742349841026 1101 36-37 1.1136941859905392 1101 38-39 1.188043815990632 1101 40-41 1.0711660386647708 1101 42-43 1.1399696024802282 1101 44-45 1.0121264909959464 1101 46-47 0.9275796063635298 1101 48-49 0.918229424159172 1101 50-51 0.8310752164160249 1101 52-53 0.8232302590116802 1101 54-55 0.9551806916230206 1101 56-57 0.9232130169069492 1101 58-59 0.9680496164237198 1101 60-61 1.045652709348957 1101 62-63 1.0043448755928992 1101 64-65 0.9816728019060506 1101 66-67 0.9411814832648204 1101 68-69 0.9279108602438058 1101 70-71 1.0082974684590873 1101 72-73 1.0107608456611032 1101 74-75 0.9521029288519465 1101 76-77 1.010994486271617 1101 78-79 0.9931198551508693 1101 80-81 0.966822877515682 1101 82-83 0.9514832293058468 1101 84-85 0.8972214361831341 1101 86-87 0.8948202191008008 1101 88-89 0.9005791626531234 1101 90-91 0.9031620742535118 1101 92-93 0.8875327145300815 1101 94-95 0.9091250729149465 1101 96-97 0.9931055163848406 1101 98-99 0.9991094181920559 1101 100 1.0095825141700523 1102 1 0.3963462699987481 1102 2 0.46703641207970037 1102 3 0.4533049662530715 1102 4 0.37229478714827025 1102 5 0.3384356804841886 1102 6 0.3291412262250262 1102 7 0.2861885110954816 1102 8 0.4543861987196465 1102 9 0.5747809169938876 1102 10-11 0.4687687945374748 1102 12-13 0.48382909522023 1102 14-15 0.5665389898076683 1102 16-17 0.5707113749519621 1102 18-19 0.521326360026741 1102 20-21 0.5007911728216143 1102 22-23 0.45465468700651 1102 24-25 0.55079545913798 1102 26-27 0.6029100404419125 1102 28-29 0.6527482772746396 1102 30-31 0.6236848847049217 1102 32-33 0.6005791801724456 1102 34-35 0.5996197741221394 1102 36-37 0.6239214065608394 1102 38-39 0.6028476253070636 1102 40-41 0.6302968447695321 1102 42-43 0.6237908752945742 1102 44-45 0.5135748969576639 1102 46-47 0.4991676572137962 1102 48-49 0.4752908617174043 1102 50-51 0.43126068671124074 1102 52-53 0.5273624263718872 1102 54-55 0.5199891479126393 1102 56-57 0.49540426214568356 1102 58-59 0.5025071232288454 1102 60-61 0.49017866128950516 1102 62-63 0.45525352607272396 1102 64-65 0.3766035099537177 1102 66-67 0.4434236934309368 1102 68-69 0.43532378580517417 1102 70-71 0.44390400475218783 1102 72-73 0.45484815057549 1102 74-75 0.4024955719381573 1102 76-77 0.4109919152950958 1102 78-79 0.4228021148023231 1102 80-81 0.4071972618038977 1102 82-83 0.4037174407432005 1102 84-85 0.4206034217811414 1102 86-87 0.35796201127769933 1102 88-89 0.38673799310738843 1102 90-91 0.38896393544106544 1102 92-93 0.34428991094755546 1102 94-95 0.43946424990522814 1102 96-97 0.4761654323238851 1102 98-99 0.5402413576182958 1102 100 0.482086820190883 1103 1 0.4477019660853472 1103 2 0.504911995749751 1103 3 0.4945117585279952 1103 4 0.47472424828335136 1103 5 0.4096921869685488 1103 6 0.5130912818360187 1103 7 0.5400419835563284 1103 8 0.537972391730527 1103 9 0.6563964009570569 1103 10-11 0.7369058840534279 1103 12-13 0.6525188752920741 1103 14-15 0.8156354453136387 1103 16-17 0.7838458947114475 1103 18-19 0.7159816536105907 1103 20-21 0.6931978852581651 1103 22-23 0.668459875561787 1103 24-25 0.6802949134349277 1103 26-27 0.7097634495880385 1103 28-29 0.7940793013866028 1103 30-31 0.7187051901348482 1103 32-33 0.769538195300278 1103 34-35 0.7751700098461782 1103 36-37 0.7819840242302405 1103 38-39 0.7775857311481786 1103 40-41 0.768942350081538 1103 42-43 0.729666469631141 1103 44-45 0.7016360909307693 1103 46-47 0.7008103544718978 1103 48-49 0.6628227063909904 1103 50-51 0.6416958471623744 1103 52-53 0.678441274532922 1103 54-55 0.8310701138012462 1103 56-57 0.7696159897358754 1103 58-59 0.7477410739558294 1103 60-61 0.7996238277556529 1103 62-63 0.7953174958878222 1103 64-65 0.7939819638006682 1103 66-67 0.8299348755351943 1103 68-69 0.7381053551375985 1103 70-71 0.8009231165238155 1103 72-73 0.7712430811406179 1103 74-75 0.7588351890825606 1103 76-77 0.807609653739032 1103 78-79 0.7689363333744197 1103 80-81 0.75607649094351 1103 82-83 0.7916398473369384 1103 84-85 0.7665962028645801 1103 86-87 0.6822181972239036 1103 88-89 0.7840611491086094 1103 90-91 0.7723229152089353 1103 92-93 0.8066908188660307 1103 94-95 0.7889853930929931 1103 96-97 0.7736594200801896 1103 98-99 0.802170831626352 1103 100 0.8849293184382816 1104 1 0.41842649034623136 1104 2 0.48074055771797575 1104 3 0.3971445582333075 1104 4 0.49758062283537896 1104 5 0.37221267376918377 1104 6 0.46912599615678374 1104 7 0.47932699208882923 1104 8 0.44874389510968626 1104 9 0.5854617272398173 1104 10-11 0.643010827660639 1104 12-13 0.5322066963148586 1104 14-15 0.5780030097954381 1104 16-17 0.6712528391216779 1104 18-19 0.6307580704894775 1104 20-21 0.5838077607388144 1104 22-23 0.5867364899722318 1104 24-25 0.5595872632905952 1104 26-27 0.742890286293175 1104 28-29 0.7525938545555277 1104 30-31 0.6648764409610735 1104 32-33 0.6966809014959878 1104 34-35 0.681746923184761 1104 36-37 0.637315241930736 1104 38-39 0.6406580504130446 1104 40-41 0.6351492855780663 1104 42-43 0.5413338733242767 1104 44-45 0.629051172367113 1104 46-47 0.556565765722354 1104 48-49 0.57402685908081 1104 50-51 0.5470468905613046 1104 52-53 0.48774513442445766 1104 54-55 0.5241790134123008 1104 56-57 0.4858480850876603 1104 58-59 0.5430535062658208 1104 60-61 0.49354372096189536 1104 62-63 0.5221179432678298 1104 64-65 0.5189919435252079 1104 66-67 0.5524412133318606 1104 68-69 0.547993013895919 1104 70-71 0.56346085035592 1104 72-73 0.545240875705943 1104 74-75 0.6065655620321593 1104 76-77 0.5179390840549338 1104 78-79 0.5733883095162966 1104 80-81 0.5757299978999804 1104 82-83 0.5536727518528757 1104 84-85 0.5165645957751721 1104 86-87 0.5299254698513884 1104 88-89 0.556717810154499 1104 90-91 0.4925847132677319 1104 92-93 0.5542483678552017 1104 94-95 0.5596754210239787 1104 96-97 0.5939696653405484 1104 98-99 0.6010593932921608 1104 100 0.6412685942973653 1105 1 0.3485861384892175 1105 2 0.4177640241781937 1105 3 0.3580216614972791 1105 4 0.33020951111355146 1105 5 0.2510832886158667 1105 6 0.3557425332097566 1105 7 0.3442338616444047 1105 8 0.247785989429552 1105 9 0.3878237965778055 1105 10-11 0.4941232365417818 1105 12-13 0.40996112392213746 1105 14-15 0.536832893630077 1105 16-17 0.5434616239839443 1105 18-19 0.49716195126848106 1105 20-21 0.499912037849235 1105 22-23 0.48041529215607426 1105 24-25 0.48526966755359524 1105 26-27 0.5395287626147578 1105 28-29 0.6154625466229078 1105 30-31 0.6389098563454993 1105 32-33 0.6310224529540776 1105 34-35 0.6345728806256119 1105 36-37 0.5647320941434941 1105 38-39 0.5102586724100533 1105 40-41 0.5100256644242904 1105 42-43 0.6124680306746768 1105 44-45 0.4949795488603499 1105 46-47 0.44440983759110964 1105 48-49 0.46630794538514664 1105 50-51 0.49412838215710053 1105 52-53 0.48509970813711334 1105 54-55 0.5714579734770737 1105 56-57 0.5240111083869579 1105 58-59 0.5312259278716382 1105 60-61 0.5199282712438489 1105 62-63 0.5019745458282827 1105 64-65 0.57318563124538 1105 66-67 0.47661705533689513 1105 68-69 0.51411911340481 1105 70-71 0.49437752035751714 1105 72-73 0.496835546534129 1105 74-75 0.511407121989901 1105 76-77 0.5242756033684337 1105 78-79 0.5633675699373057 1105 80-81 0.6031161119397339 1105 82-83 0.4836778016165333 1105 84-85 0.4812363040304888 1105 86-87 0.4749170777790077 1105 88-89 0.5598714997800087 1105 90-91 0.5344621040145228 1105 92-93 0.4545348811835339 1105 94-95 0.5089611430883849 1105 96-97 0.5825348192886146 1105 98-99 0.5449371934385674 1105 100 0.5196429537693525 1106 1 0.2832495061902449 1106 2 0.34781564341007964 1106 3 0.33744937912609174 1106 4 0.395749877276522 1106 5 0.2723212910755137 1106 6 0.36276945324142673 1106 7 0.4261678290497599 1106 8 0.42874997450645225 1106 9 0.57542218152863 1106 10-11 0.5513197127855136 1106 12-13 0.5327374179348112 1106 14-15 0.5862027873751927 1106 16-17 0.5178087405824527 1106 18-19 0.5535819457111941 1106 20-21 0.6237729093364024 1106 22-23 0.5582028057412245 1106 24-25 0.5298133076032556 1106 26-27 0.5733504172313815 1106 28-29 0.7033694134108543 1106 30-31 0.6965378704612206 1106 32-33 0.6502305037805058 1106 34-35 0.5662427750128955 1106 36-37 0.5624457233423925 1106 38-39 0.6261637592873299 1106 40-41 0.680386810738483 1106 42-43 0.6126361203326809 1106 44-45 0.5556992042947755 1106 46-47 0.6028345572015965 1106 48-49 0.5635058335125862 1106 50-51 0.552348387308264 1106 52-53 0.5544608598315577 1106 54-55 0.6353274117226491 1106 56-57 0.5444959507263221 1106 58-59 0.6057191184615363 1106 60-61 0.6433609566726943 1106 62-63 0.6591379747080452 1106 64-65 0.6670749104921967 1106 66-67 0.657183497959231 1106 68-69 0.6719341934335432 1106 70-71 0.6591843317982331 1106 72-73 0.6858858417019995 1106 74-75 0.7166691852214662 1106 76-77 0.7531674321630852 1106 78-79 0.7554471995707921 1106 80-81 0.6849017643029001 1106 82-83 0.6812428641160366 1106 84-85 0.7235765095146718 1106 86-87 0.7025031961766075 1106 88-89 0.6724922106300468 1106 90-91 0.6360699267180294 1106 92-93 0.6029509116781391 1106 94-95 0.6732767999327862 1106 96-97 0.7577367329767917 1106 98-99 0.7836102869963852 1106 100 0.790256672801199 1107 1 0.5087119533341884 1107 2 0.41481061358217985 1107 3 0.4593317491775686 1107 4 0.5223896650391069 1107 5 0.442924099080777 1107 6 0.5503266793991983 1107 7 0.5240576726137647 1107 8 0.48594352291876675 1107 9 0.636681305204867 1107 10-11 0.6584354392218756 1107 12-13 0.582426809260717 1107 14-15 0.6673027384854464 1107 16-17 0.6567566140030294 1107 18-19 0.7082446906345083 1107 20-21 0.7866287277182877 1107 22-23 0.7298411168394523 1107 24-25 0.798677369995545 1107 26-27 0.7875948525814067 1107 28-29 0.8721894389021543 1107 30-31 0.8435203022433697 1107 32-33 0.878086459170305 1107 34-35 0.8175292603427664 1107 36-37 0.7495613445668212 1107 38-39 0.8195090364997668 1107 40-41 0.8238304980865436 1107 42-43 0.8421097409846112 1107 44-45 0.850393114769254 1107 46-47 0.8372495896976133 1107 48-49 0.864329279617273 1107 50-51 0.8152920175186367 1107 52-53 0.8394941276144721 1107 54-55 0.8313777977627907 1107 56-57 0.8735754047760764 1107 58-59 0.8163341327419431 1107 60-61 0.7886264479107226 1107 62-63 0.8427271156612193 1107 64-65 0.8638580300857868 1107 66-67 0.880177869479617 1107 68-69 0.916207933266513 1107 70-71 0.862055440982008 1107 72-73 0.8519128810437238 1107 74-75 0.8488779909495427 1107 76-77 0.8858850580425717 1107 78-79 0.8852564336339981 1107 80-81 0.8629997504639775 1107 82-83 0.9182116101065461 1107 84-85 0.8481988583348858 1107 86-87 0.7964792823478035 1107 88-89 0.7859136604417003 1107 90-91 0.7940537667979051 1107 92-93 0.7624300606048138 1107 94-95 0.7811742320663839 1107 96-97 0.7842565854662702 1107 98-99 0.7512255554967311 1107 100 0.7301611974026336 1108 1 0.27398857586581826 1108 2 0.34544748174464956 1108 3 0.30716200754390144 1108 4 0.31393603425269845 1108 5 0.3088635602077048 1108 6 0.4138751512879466 1108 7 0.37422077266590037 1108 8 0.3712776682325014 1108 9 0.3901040509820035 1108 10-11 0.4577572672392307 1108 12-13 0.3598701804946387 1108 14-15 0.4887670384466105 1108 16-17 0.4475050339049176 1108 18-19 0.4769778091747483 1108 20-21 0.5093648640067059 1108 22-23 0.5302080471601442 1108 24-25 0.5628131756653048 1108 26-27 0.6214083192520974 1108 28-29 0.5993934388074393 1108 30-31 0.6159178973500943 1108 32-33 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-2.1119317524659422 2216 7 -2.155234008628593 2216 8 -2.2428812106878055 2216 9 -2.5977271683795315 2216 10-11 -2.7340665966171542 2216 12-13 -2.653645285233729 2216 14-15 -3.0958076572767297 2216 16-17 -2.9987431245651237 2216 18-19 -2.799482831632531 2216 20-21 -2.9125744765910824 2216 22-23 -2.756067527086117 2216 24-25 -2.957281836450939 2216 26-27 -3.094319872611898 2216 28-29 -6.304732708617394 2216 30-31 -4.205569456604273 2216 32-33 -3.6487675142022127 2216 34-35 -3.3751248688452797 2216 36-37 -3.421419002841482 2216 38-39 -3.3917811292896047 2216 40-41 -3.392690736717622 2216 42-43 -3.635859581711081 2216 44-45 -3.405033831178528 2216 46-47 -3.382097534233459 2216 48-49 -3.3873696049637516 2216 50-51 -3.322428916487617 2216 52-53 -3.2883257243856647 2216 54-55 -3.414778302251719 2216 56-57 -3.219266664878173 2216 58-59 -3.4327571812010476 2216 60-61 -3.348065439997182 2216 62-63 -3.445752753972009 2216 64-65 -3.3794405308435884 2216 66-67 -3.4727273626215585 2216 68-69 -3.4055789160479684 2216 70-71 -3.4680570942579685 2216 72-73 -3.460119067511073 2216 74-75 -3.4464428595885117 2216 76-77 -3.2605958239782815 2216 78-79 -3.136967477397924 2216 80-81 -3.1941505402760555 2216 82-83 -3.231406522643198 2216 84-85 -3.140156103573613 2216 86-87 -3.239636265758108 2216 88-89 -3.1518588337670543 2216 90-91 -3.3265067585292876 2216 92-93 -3.3559067126253126 2216 94-95 -3.282641925794472 2216 96-97 -3.3026912846091427 2216 98-99 -3.534531127525536 2216 100 -3.57920298622129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 180007.0 3 34693.0 4 23243.0 5 20508.0 6 14314.0 7 12941.0 8 13554.0 9 14879.0 10 15782.0 11 16196.0 12 17571.0 13 18736.0 14 19642.0 15 19851.0 16 19949.0 17 19243.0 18 18980.0 19 18503.0 20 18285.0 21 19313.0 22 23226.0 23 36044.0 24 55666.0 25 65859.0 26 68226.0 27 56611.0 28 58709.0 29 69720.0 30 80686.0 31 94203.0 32 117679.0 33 151119.0 34 204630.0 35 300174.0 36 476905.0 37 796661.0 38 919857.0 39 328880.0 40 853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8246402867574 20.95492734811326 13.038072583214468 22.182359781914876 2 17.047335021323523 22.045630670568585 37.883664664823776 23.023369643284113 3 17.331270973980725 27.542854183763872 29.586548733934254 25.53932610832115 4 12.420889246521154 19.758472139731147 37.36303792498038 30.45760068876732 5 11.835339226994513 38.88814464499003 32.52384613739292 16.752669990622525 6 28.266072616266946 39.65448530117031 18.278487693222864 13.800954389339875 7 25.68208248009934 34.3740029967792 21.848299029480337 18.09561549364113 8 23.899372201994076 36.451279686668364 21.13108559252658 18.518262518810975 9 23.99834654402034 19.875220809770163 21.521787389516316 34.60464525669319 10-11 22.281445968997197 29.4415417941677 27.7006045415079 20.5764076953272 12-13 23.613650079515256 27.357221794260877 27.332838691818413 21.696289434405458 14-15 21.704521059443437 29.477625927925978 25.040140752395807 23.77771226023478 16-17 20.56742410793738 32.05623584645157 25.11672086717511 22.259619178435937 18-19 20.402202065539637 30.27425856348815 27.521001213963824 21.80253815700839 20-21 23.00626850029353 29.81690935974843 26.231250643763783 20.945571496194265 22-23 22.057499328231533 30.484385871912696 26.371665655965167 21.086449143890608 24-25 21.49384158901345 29.87670795238435 26.99410736868222 21.63534308991998 26-27 21.30835884295063 29.347791703735492 27.284054002301634 22.05979545101225 28-29 21.594980051181647 29.512508948794824 27.075048063711026 21.817462936312502 30-31 22.181514159085125 30.62324650043053 26.129784774971753 21.065454565512596 32-33 20.953557152629866 29.863529733848594 27.359321569497553 21.82359154402399 34-35 20.971636311240164 28.84105291181889 27.683766230476852 22.503544546464095 36-37 22.36373258189351 30.090722804913046 26.291016549980284 21.25452806321316 38-39 21.94361135607824 29.970704507953034 26.831638050728575 21.254046085240155 40-41 21.181208896839898 29.476537350073826 27.382009073441154 21.96024467964512 42-43 21.0782098743335 29.55219391234437 27.212627122593563 22.15696909072857 44-45 21.92724385626631 29.687526207586483 26.951794701151055 21.433435234996146 46-47 21.39285163086531 30.69951253086012 26.486359858560437 21.42127597971413 48-49 21.069815464126883 29.561345131117594 27.476665665557043 21.892173739198483 50-51 21.222721452913657 29.036547303827813 27.5410465713418 22.19968467191673 52-53 21.276182594249686 29.22445465616057 27.243834002853383 22.25552874673636 54-55 21.6250915195075 30.284559251958044 26.801380830119083 21.288968398415374 56-57 20.870948823271828 29.806405786888774 26.885100855178067 22.43754453466133 58-59 20.66425802431141 28.964325812404613 27.662179804821623 22.709236358462356 60-61 21.76724370831585 29.919540467961177 26.554797493592595 21.75841833013038 62-63 21.455164866288094 29.70257675407739 26.802008937466326 22.04024944216819 64-65 21.215731968959144 29.107956434194204 27.51922717782594 22.157084419020716 66-67 21.09860812382359 29.2831399237033 26.839873621556066 22.77837833091705 68-69 22.15006813788054 29.7134629330155 26.633930165482855 21.502538763621104 70-71 22.16752689019345 29.06780662700445 27.07391918589271 21.69074729690939 72-73 21.372293954185352 29.266539645145727 27.831716521825662 21.529449878843263 74-75 21.273817006860305 29.12545950005673 27.57211967083496 22.028603822247998 76-77 21.48680500686718 29.382964642886932 26.89687770408234 22.23335264616355 78-79 21.780973176131983 29.672541211096704 27.047496873124675 21.49898873964664 80-81 21.24854377871988 29.157014340282934 27.486039468732653 22.10840241226453 82-83 21.045887066962223 29.28693836622752 27.563398590396993 22.10377597641326 84-85 21.36036110776848 29.1837061139839 27.26724318524356 22.18868959300406 86-87 21.79140660379493 29.342678955543754 27.05826440192134 21.807650038739972 88-89 21.127283918431598 29.20215326247046 27.387918640520613 22.28264417857733 90-91 20.95107431102462 28.905537130747444 27.38572872974037 22.75765982848756 92-93 21.428321368368028 29.442527360887933 26.8779404687865 22.251210801957544 94-95 21.339612448921493 29.080266634277503 27.26003078246514 22.320090134335864 96-97 21.208141825344715 29.074483835995956 27.442127168546204 22.275247170113126 98-99 21.06368505495572 29.071490893282427 27.438528066358632 22.42629598540323 100 21.4829625390413 29.40754937613848 27.004656475986717 22.104831608833504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29513.0 1 22274.0 2 12361.0 3 7839.0 4 5039.5 5 3622.5 6 2992.5 7 2938.5 8 2997.5 9 2878.5 10 2629.0 11 2343.5 12 2240.0 13 2284.0 14 2347.5 15 2492.0 16 2774.5 17 3285.0 18 3954.5 19 4931.0 20 6410.0 21 8441.5 22 11792.5 23 17139.0 24 23246.0 25 29770.0 26 38657.5 27 49607.5 28 61666.5 29 74063.5 30 83607.0 31 91533.0 32 99973.5 33 107600.0 34 115160.0 35 121652.5 36 125390.5 37 128348.5 38 130877.0 39 131559.0 40 131644.0 41 130836.5 42 128250.0 43 126812.0 44 126723.0 45 126302.5 46 127901.5 47 131348.5 48 135614.0 49 139425.5 50 140640.5 51 137264.0 52 130032.5 53 123451.0 54 119518.5 55 117527.5 56 112462.5 57 104499.5 58 97736.0 59 90508.0 60 79689.5 61 67351.0 62 55816.5 63 44833.0 64 35212.5 65 27461.0 66 21113.0 67 15731.0 68 11828.0 69 8943.5 70 6791.0 71 5305.0 72 4215.5 73 3366.5 74 2704.5 75 2056.0 76 1549.0 77 1269.5 78 1043.0 79 861.0 80 636.5 81 414.5 82 306.0 83 251.5 84 204.5 85 155.5 86 135.0 87 115.5 88 97.0 89 80.5 90 73.5 91 58.5 92 39.0 93 31.0 94 23.5 95 22.5 96 20.0 97 15.5 98 17.0 99 19.5 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011549117066623323 2 0.007294179199972624 3 0.008622440227128133 4 0.0076543855802181865 5 0.009072698202435085 6 0.011796758953042146 7 0.010423472128355942 8 0.007699411377748881 9 0.0065737664394815015 10-11 0.006022200419730484 12-13 0.0066975873826909125 14-15 0.007643129130835513 16-17 0.004761478088871019 18-19 0.005628224691336902 20-21 0.005526916646892838 22-23 0.005932148824669094 24-25 0.005605711792571554 26-27 0.006134764913557222 28-29 0.01480223093821605 30-31 0.005504403748127489 32-33 0.005279274760474014 34-35 0.004851529683932409 36-37 0.006562509990098828 38-39 0.00583084078022503 40-41 0.005707019837015618 42-43 0.004975350627141821 44-45 0.004446297506156153 46-47 0.0063824067999760465 48-49 0.003602063802455617 50-51 0.003939757283935831 52-53 0.0035795509036902697 54-55 0.0049978635259071685 56-57 0.0029942155357912314 58-59 0.0025889833580149744 60-61 0.003647089599986312 62-63 0.003309396118506098 64-65 0.004581374898748237 66-67 0.0035908073530729434 68-69 0.0038046798913437454 70-71 0.005020376424672516 72-73 0.0029491897382605365 74-75 0.002566470459249627 76-77 0.0026677785036936913 78-79 0.003680858948134333 80-81 0.004041065328379895 82-83 0.0018010319012278086 84-85 0.002735317199989734 86-87 0.002330085022213477 88-89 0.0018010319012278086 90-91 0.0027240607506070604 92-93 0.0024876753135709105 94-95 0.001879827046906525 96-97 0.0015421335654263108 98-99 0.002127468933325349 100 0.002859138143199146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4441898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.68649596808778 #Duplication Level Percentage of deduplicated Percentage of total 1 79.83333109051807 54.03638442977369 2 13.236416022149648 17.918532394303295 3 3.59060383618968 7.2910617624375975 4 1.3119434622595392 3.552034234743577 5 0.6145931980257167 2.0799830010090927 6 0.3404147771768682 1.3824890065715754 7 0.2134211577265733 1.011201123237503 8 0.15206642043813653 0.8234274523093977 9 0.11056411197013681 0.6735327587173685 >10 0.546779157421681 6.503154255491907 >50 0.03369247703140551 1.5642702795275354 >100 0.015408437952119193 1.7513055153996804 >500 5.327661745575183E-4 0.23931026504715514 >1k 1.664892613551144E-4 0.21332358440117946 >5k 3.3297852271017326E-5 0.12260753292575793 >10k+ 3.3297852271017326E-5 0.8373824041036048 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37154 0.8364442407277249 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5440 0.12247016928349098 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.005159506139042E-5 0.0 5 0.0 0.0 0.0 1.1256449382673803E-4 0.0 6 0.0 0.0 0.0 1.1256449382673803E-4 0.0 7 0.0 0.0 0.0 1.3507739259208563E-4 0.0 8 0.0 0.0 0.0 1.3507739259208563E-4 0.0 9 0.0 0.0 0.0 1.5759029135743323E-4 0.0 10-11 0.0 0.0 0.0 1.6884674074010703E-4 0.0 12-13 0.0 0.0 0.0 2.0261608888812847E-4 0.0 14-15 0.0 0.0 0.0 2.588983358014975E-4 0.0 16-17 0.0 0.0 0.0 3.0392413333219263E-4 0.0 18-19 0.0 0.0 0.0 3.4894993086288784E-4 0.0 20-21 0.0 0.0 0.0 4.3900152592427836E-4 0.0 22-23 0.0 0.0 0.0 6.978998617257758E-4 0.0 24-25 0.0 0.0 0.0 0.0012382094320941184 0.0 26-27 0.0 0.0 0.0 0.0017672625530797872 0.0 28-29 0.0 0.0 0.0 0.003320652567888772 0.0 30-31 0.0 0.0 0.0 0.007823232320958294 0.0 32-33 0.0 0.0 0.0 0.01781895937277263 0.0 34-35 0.0 0.0 0.0 0.03230600972827381 0.0 36-37 0.0 1.1256449382673802E-5 0.0 0.05198228324918762 0.0 38-39 0.0 2.2512898765347604E-5 0.0 0.08405190754042528 0.0 40-41 0.0 2.2512898765347604E-5 0.0 0.13488603295258017 0.0 42-43 0.0 2.2512898765347604E-5 0.0 0.18906332383138919 0.0 44-45 0.0 2.2512898765347604E-5 0.0 0.24249768905094174 0.0 46-47 0.0 2.2512898765347604E-5 0.0 0.3038678510852793 0.0 48-49 0.0 2.2512898765347604E-5 0.0 0.3799276795640062 0.0 50-51 0.0 2.2512898765347604E-5 0.0 0.4668499816970133 0.0 52-53 0.0 2.2512898765347604E-5 0.0 0.5588039167040756 0.0 54-55 0.0 2.2512898765347604E-5 0.0 0.6577706196765436 1.1256449382673802E-5 56-57 0.0 2.2512898765347604E-5 0.0 0.7640652711971324 2.2512898765347604E-5 58-59 0.0 2.2512898765347604E-5 0.0 0.8829896589250812 2.2512898765347604E-5 60-61 0.0 2.2512898765347604E-5 0.0 1.010153767601147 3.3769348148021406E-5 62-63 0.0 2.2512898765347604E-5 0.0 1.1407173239907804 4.502579753069521E-5 64-65 0.0 2.2512898765347604E-5 0.0 1.292015260143299 4.502579753069521E-5 66-67 0.0 2.2512898765347604E-5 0.0 1.4695857491549784 4.502579753069521E-5 68-69 0.0 2.2512898765347604E-5 0.0 1.6536174401123125 4.502579753069521E-5 70-71 0.0 2.2512898765347604E-5 0.0 1.8596892589609215 4.502579753069521E-5 72-73 0.0 2.2512898765347604E-5 0.0 2.110392899611832 4.502579753069521E-5 74-75 0.0 2.2512898765347604E-5 0.0 2.3532620514924023 4.502579753069521E-5 76-77 0.0 2.2512898765347604E-5 0.0 2.5896362320791697 4.502579753069521E-5 78-79 0.0 2.2512898765347604E-5 0.0 2.8575847531843372 4.502579753069521E-5 80-81 0.0 2.2512898765347604E-5 0.0 3.155295326457294 4.502579753069521E-5 82-83 0.0 2.2512898765347604E-5 0.0 3.4780402431573174 4.502579753069521E-5 84-85 0.0 2.2512898765347604E-5 0.0 3.7981286378030292 4.502579753069521E-5 86-87 0.0 2.2512898765347604E-5 0.0 4.139840671712857 4.502579753069521E-5 88 0.0 2.2512898765347604E-5 0.0 4.4190118728525505 4.502579753069521E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5680 0.0 45.4325 1 TCTGTCG 1705 0.0 36.938477 8 GTATCAA 8835 0.0 33.46468 1 GTCTCGC 1805 0.0 31.249756 1 ATAACGC 345 0.0 28.610678 3 CGCTCTG 1695 0.0 28.284824 5 TACCTGG 3725 0.0 27.760311 2 ATCAACG 10225 0.0 27.719276 3 GTACCTG 3990 0.0 27.448942 1 CTGTCGC 2235 0.0 27.337854 9 TCAACGC 10365 0.0 27.299526 4 TCTCGCT 2005 0.0 26.959473 2 TCGCTCT 1930 0.0 26.78942 4 TATCAAC 11155 0.0 25.745394 2 GTACATG 17935 0.0 25.39598 1 CAACGCA 11335 0.0 25.128937 5 CTCGCTC 2010 0.0 25.021633 3 CATGGGT 7495 0.0 24.26986 4 TACATGG 17765 0.0 24.235847 2 AACGCAG 11900 0.0 24.092749 6 >>END_MODULE