FastQCFastQC Report
Wed 25 May 2016
SRR2431429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441898
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315180.7095615432862259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259820.5849301357212615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243850.5489770363930013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220640.4967245983586296No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97360.2191855823794243No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.1325559479303667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53230.1198361601279453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115050.060.071231
TATAACG4750.045.5453342
GTATCAA217500.043.8888441
ATAACGC4900.041.271673
GTGGTAT40450.032.9154051
TGGTATC40150.032.5640872
TAACGCA6850.031.582534
ATCAACG303550.031.2039013
TATCAAC303750.031.1369042
TATCACG5600.031.0737272
TCAACGC305800.031.0050684
TCTGTCG17750.030.9829378
CAACGCA318400.029.8066435
GTCTCGC17250.029.728211
AACGCAG329800.028.8751286
TACCTGG35700.027.9284782
GTACCTG35800.027.728781
CGCTCTG17850.027.3870285
TCGCTCT20650.026.4190734
ACGCAGA368850.025.716187
Produced by FastQC (version 0.11.5)