##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431429_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4441898
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25357921321021	33.0	31.0	34.0	30.0	34.0
2	32.36593523759438	34.0	31.0	34.0	31.0	34.0
3	32.46541793620655	34.0	31.0	34.0	30.0	34.0
4	36.032825607431775	37.0	35.0	37.0	35.0	37.0
5	35.957243277535866	37.0	35.0	37.0	35.0	37.0
6	36.00398095588868	37.0	35.0	37.0	35.0	37.0
7	35.93841101258966	37.0	35.0	37.0	35.0	37.0
8	35.91828695751231	37.0	35.0	37.0	35.0	37.0
9	37.602770932605836	39.0	37.0	39.0	35.0	39.0
10-11	37.535562275405695	39.0	37.0	39.0	34.5	39.0
12-13	37.51799320920922	39.0	37.0	39.0	35.0	39.0
14-15	38.840182620132204	40.0	38.0	41.0	34.5	41.0
16-17	38.90583102088342	40.0	38.0	41.0	35.0	41.0
18-19	38.9916122567425	40.5	38.5	41.0	35.0	41.0
20-21	38.9842657350529	40.0	38.5	41.0	35.0	41.0
22-23	38.88465673907865	40.0	38.0	41.0	35.0	41.0
24-25	38.82061238236447	40.0	38.0	41.0	35.0	41.0
26-27	38.667151519463076	40.0	38.0	41.0	34.0	41.0
28-29	38.55269234007625	40.0	38.0	41.0	34.0	41.0
30-31	38.322664545651435	40.0	38.0	41.0	34.0	41.0
32-33	38.05298286903481	40.0	37.0	41.0	33.5	41.0
34-35	37.85628857303792	40.0	37.0	41.0	33.0	41.0
36-37	37.67114879720336	40.0	37.0	41.0	33.0	41.0
38-39	37.52623810812405	40.0	37.0	41.0	33.0	41.0
40-41	37.38019693383324	40.0	37.0	41.0	33.0	41.0
42-43	37.22921519134388	40.0	37.0	41.0	32.0	41.0
44-45	36.97100529998663	40.0	36.0	41.0	31.0	41.0
46-47	36.593646004478266	40.0	36.0	41.0	30.0	41.0
48-49	36.29068711168064	40.0	36.0	41.0	30.0	41.0
50-51	36.14544199349017	40.0	36.0	41.0	29.0	41.0
52-53	35.993008506723925	40.0	35.0	41.0	28.5	41.0
54-55	35.81624690616489	40.0	35.0	41.0	28.0	41.0
56-57	35.60824393086018	39.0	35.0	41.0	28.0	41.0
58-59	35.44167234366931	39.0	35.0	41.0	27.5	41.0
60-61	35.206143409866684	39.0	35.0	41.0	27.0	41.0
62-63	34.942086693571085	38.0	35.0	41.0	26.0	41.0
64-65	34.67272796448725	37.5	35.0	40.0	26.0	41.0
66-67	34.40563099377788	37.0	34.5	40.0	26.0	41.0
68-69	34.09791557572912	37.0	34.0	40.0	26.0	41.0
70-71	33.749745604243955	36.0	34.0	39.0	25.5	41.0
72-73	33.42238362970063	36.0	34.0	39.0	25.0	41.0
74-75	33.127746742496115	35.0	34.0	39.0	24.5	40.5
76-77	32.79374909104172	35.0	34.0	37.5	24.0	40.0
78-79	32.49805387246623	35.0	33.0	37.0	23.5	39.0
80-81	32.21182375191866	35.0	33.0	36.5	23.0	39.0
82-83	31.975829701627546	35.0	33.0	36.0	23.0	39.0
84-85	31.759975465442928	35.0	33.0	36.0	22.5	37.0
86-87	31.527653719198412	35.0	33.0	35.5	21.5	37.0
88-89	31.39350588419635	35.0	33.0	35.0	20.5	36.5
90-91	31.218784289958933	35.0	33.0	35.0	20.0	36.0
92-93	31.023650925797938	35.0	33.0	35.0	19.0	36.0
94-95	30.85961980666823	35.0	33.0	35.0	17.5	36.0
96-97	30.689969580571187	35.0	33.0	35.0	6.5	36.0
98-99	30.5315031997583	35.0	33.0	35.0	2.0	35.0
100	30.42397416599841	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18172844139576227
1101	2	0.2283035518497698
1101	3	0.22637698144874463
1101	4	0.17417767606075074
1101	5	0.16474202902755763
1101	6	0.13541827201099466
1101	7	0.18703794165205778
1101	8	0.22054494556032012
1101	9	0.2856157917267339
1101	10-11	0.26828319916290155
1101	12-13	0.31969376403361593
1101	14-15	0.4326382594503073
1101	16-17	0.4032817993639881
1101	18-19	0.3806549055469901
1101	20-21	0.3405190762125727
1101	22-23	0.32577846033295543
1101	24-25	0.33784943288464575
1101	26-27	0.3362776227740696
1101	28-29	0.3474437552967302
1101	30-31	0.3794279871473236
1101	32-33	0.39447420201911143
1101	34-35	0.4735735307696558
1101	36-37	0.4811164486296491
1101	38-39	0.46212403217074893
1101	40-41	0.5478293381224546
1101	42-43	0.5555028957380657
1101	44-45	0.5369444280274465
1101	46-47	0.4765811169343763
1101	48-49	0.4451087587310383
1101	50-51	0.4500288427601191
1101	52-53	0.4825209820767
1101	54-55	0.4997529288437974
1101	56-57	0.47225692860791213
1101	58-59	0.5101094434762672
1101	60-61	0.5694784052761293
1101	62-63	0.5855999449391405
1101	64-65	0.5017992706963597
1101	66-67	0.5106252720287046
1101	68-69	0.498332557872196
1101	70-71	0.5489798987415497
1101	72-73	0.5342342903845108
1101	74-75	0.503456027780004
1101	76-77	0.5865485811748599
1101	78-79	0.5297648336639327
1101	80-81	0.5377223618433362
1101	82-83	0.4871155447158877
1101	84-85	0.49874940625635134
1101	86-87	0.5111245682278671
1101	88-89	0.47973955061897655
1101	90-91	0.5261603591141863
1101	92-93	0.5776158588942941
1101	94-95	0.5792009972018626
1101	96-97	0.5974131139327525
1101	98-99	0.6800967706679444
1101	100	0.7119990392986892
1102	1	0.14707034004563013
1102	2	0.2309799213867123
1102	3	0.1984225128835746
1102	4	0.14429801551818855
1102	5	0.1268600641895148
1102	6	0.10201004956105919
1102	7	0.14488545589112078
1102	8	0.15139080924218717
1102	9	0.2304533620756004
1102	10-11	0.22055473475804632
1102	12-13	0.25849514607578783
1102	14-15	0.3850298063811053
1102	16-17	0.3409954661814396
1102	18-19	0.31597368462355746
1102	20-21	0.26768757998425485
1102	22-23	0.24573445066326371
1102	24-25	0.22686004790431724
1102	26-27	0.2662754047291429
1102	28-29	0.24088499959294296
1102	30-31	0.2626331085325617
1102	32-33	0.2960024444638094
1102	34-35	0.3178244417332863
1102	36-37	0.3019897157105973
1102	38-39	0.36113930924405935
1102	40-41	0.4086512508594282
1102	42-43	0.331188781560968
1102	44-45	0.29536900366369423
1102	46-47	0.25703331838227683
1102	48-49	0.33008645532725467
1102	50-51	0.28946858586872537
1102	52-53	0.26488076540734795
1102	54-55	0.25015557125309584
1102	56-57	0.36775367714960083
1102	58-59	0.28893652993256325
1102	60-61	0.28147963370184925
1102	62-63	0.2605070291907694
1102	64-65	0.28247293611082824
1102	66-67	0.296922212812305
1102	68-69	0.27023756367051277
1102	70-71	0.2494466003024769
1102	72-73	0.23860263732906617
1102	74-75	0.2168845616741919
1102	76-77	0.2553463733360033
1102	78-79	0.24366499702674815
1102	80-81	0.2352435075171755
1102	82-83	0.26166381599200506
1102	84-85	0.2382389586906868
1102	86-87	0.2064532814409823
1102	88-89	0.2328570785664219
1102	90-91	0.20124275380393897
1102	92-93	0.22152456728798242
1102	94-95	0.20686845527688646
1102	96-97	0.20067052416450437
1102	98-99	0.30084268319836127
1102	100	0.3027242159426571
1103	1	0.1272737169361733
1103	2	0.15850948550320254
1103	3	0.09894441922257613
1103	4	0.12923888162157482
1103	5	0.09124934461782885
1103	6	0.13586254079711324
1103	7	0.10098208892846117
1103	8	0.10847850763548905
1103	9	0.2202348191500434
1103	10-11	0.20609227662381358
1103	12-13	0.1784799340237413
1103	14-15	0.28668661081042757
1103	16-17	0.27153988948190744
1103	18-19	0.2728715332469136
1103	20-21	0.27742860767114763
1103	22-23	0.23542984408189938
1103	24-25	0.24291816444686987
1103	26-27	0.23720862619938288
1103	28-29	0.2647107209495019
1103	30-31	0.29482023145098424
1103	32-33	0.32281712567277054
1103	34-35	0.3119955228299105
1103	36-37	0.3346621531652403
1103	38-39	0.3169643709759171
1103	40-41	0.3272267847909589
1103	42-43	0.3613459750466248
1103	44-45	0.3675927528386609
1103	46-47	0.36502396186822494
1103	48-49	0.3775189041478768
1103	50-51	0.3329002664172407
1103	52-53	0.3507000235650608
1103	54-55	0.34009701186146657
1103	56-57	0.31979838041063147
1103	58-59	0.37964416598293127
1103	60-61	0.4117466403533854
1103	62-63	0.3590689826691502
1103	64-65	0.3331827418054374
1103	66-67	0.3305642410063072
1103	68-69	0.27189893204582205
1103	70-71	0.3027968752991015
1103	72-73	0.2925221430560754
1103	74-75	0.35469354807441533
1103	76-77	0.2983339776917191
1103	78-79	0.2863680391138601
1103	80-81	0.24296897724550348
1103	82-83	0.25975245712097816
1103	84-85	0.27539802171871486
1103	86-87	0.23358251858637757
1103	88-89	0.25034508599598126
1103	90-91	0.2557751710474321
1103	92-93	0.22123694901623736
1103	94-95	0.2457418770666031
1103	96-97	0.23343048838071212
1103	98-99	0.2821001333859243
1103	100	0.25812089447078534
1104	1	0.13516538998187855
1104	2	0.16076096213858904
1104	3	0.158027426206246
1104	4	0.12247007918919905
1104	5	0.09687369497793696
1104	6	0.10882296767837119
1104	7	0.11623122590246027
1104	8	0.14554724768792937
1104	9	0.23282030082656036
1104	10-11	0.23952959616671876
1104	12-13	0.24471509770584277
1104	14-15	0.3055282785956521
1104	16-17	0.27857498036739514
1104	18-19	0.27001315742262477
1104	20-21	0.2649466467217536
1104	22-23	0.20174368034287227
1104	24-25	0.23102050109545758
1104	26-27	0.2773648863900817
1104	28-29	0.30276985047449756
1104	30-31	0.24961641486064678
1104	32-33	0.22504637249866732
1104	34-35	0.24916381416255717
1104	36-37	0.26659571654816716
1104	38-39	0.26630279157900816
1104	40-41	0.2653051585391637
1104	42-43	0.2727977704437876
1104	44-45	0.2636902696583121
1104	46-47	0.3387180563400207
1104	48-49	0.3738281119566693
1104	50-51	0.34709677453265186
1104	52-53	0.36967925048138994
1104	54-55	0.3685881307850778
1104	56-57	0.3554663092223791
1104	58-59	0.3884730002810599
1104	60-61	0.35527561331465307
1104	62-63	0.3255514354128337
1104	64-65	0.36936906768877265
1104	66-67	0.36808822062551627
1104	68-69	0.39873071788049685
1104	70-71	0.37114900554665553
1104	72-73	0.3709815370083547
1104	74-75	0.3777018429489587
1104	76-77	0.30732879879899855
1104	78-79	0.30996739846190025
1104	80-81	0.2614702269865319
1104	82-83	0.31322194981319385
1104	84-85	0.351744030902303
1104	86-87	0.39913263190150516
1104	88-89	0.3431216016689831
1104	90-91	0.3560833891921824
1104	92-93	0.3635699691085996
1104	94-95	0.3386844135531568
1104	96-97	0.4098472176475916
1104	98-99	0.458551365783574
1104	100	0.4683965298265562
1105	1	0.10815347064493608
1105	2	0.13585509571831267
1105	3	0.09467903315286463
1105	4	0.08753033636723728
1105	5	0.07885997902831576
1105	6	0.09277676484211383
1105	7	0.15000556543569843
1105	8	0.13814983310317075
1105	9	0.19081918932076292
1105	10-11	0.13897642636539587
1105	12-13	0.06819254548879883
1105	14-15	0.24033463231125296
1105	16-17	0.1902165891626808
1105	18-19	0.23216749851058438
1105	20-21	0.1642121068695701
1105	22-23	0.14141791857095143
1105	24-25	0.15755042409929132
1105	26-27	0.22394996766971786
1105	28-29	0.18412506236890636
1105	30-31	0.21155929405198748
1105	32-33	0.17265210853791046
1105	34-35	0.2331538786961218
1105	36-37	0.24695382035859126
1105	38-39	0.23665801834779643
1105	40-41	0.25334360004760725
1105	42-43	0.2573377111658388
1105	44-45	0.3304468104900806
1105	46-47	0.2872541605343386
1105	48-49	0.20525288371461414
1105	50-51	0.22898769653883733
1105	52-53	0.26280398198996835
1105	54-55	0.212240716873211
1105	56-57	0.2610682997509315
1105	58-59	0.20771627413929394
1105	60-61	0.235094281777819
1105	62-63	0.1753939551222743
1105	64-65	0.19888318985064046
1105	66-67	0.20467642773851935
1105	68-69	0.19538475475196293
1105	70-71	0.15374311167960286
1105	72-73	0.13608727835988077
1105	74-75	0.11002979506195487
1105	76-77	0.1862087654697575
1105	78-79	0.1727959164211299
1105	80-81	0.19753339945462045
1105	82-83	0.18119586351415862
1105	84-85	0.12650559148335105
1105	86-87	0.15166822682537529
1105	88-89	0.17931923455298104
1105	90-91	0.19132809887083368
1105	92-93	0.20958575560740655
1105	94-95	0.2521901563035769
1105	96-97	0.2759975145862654
1105	98-99	0.24693647553702647
1105	100	0.2856979444509733
1106	1	0.08586501629572751
1106	2	0.16130939772015296
1106	3	0.11315292831698542
1106	4	0.12460083127803756
1106	5	0.07856506281628128
1106	6	0.11869027616130978
1106	7	0.10541492406387931
1106	8	0.10965987266368415
1106	9	0.14496787551394874
1106	10-11	0.13362546739524106
1106	12-13	0.14104205656223456
1106	14-15	0.21120768626342823
1106	16-17	0.17440979394951484
1106	18-19	0.20091532905190945
1106	20-21	0.18449187028055292
1106	22-23	0.19055838121408897
1106	24-25	0.1845438919459994
1106	26-27	0.2082371425589784
1106	28-29	0.2156306354479014
1106	30-31	0.1885775674891761
1106	32-33	0.15662923524088512
1106	34-35	0.20724764591077616
1106	36-37	0.24018633504235254
1106	38-39	0.22189315989889735
1106	40-41	0.25769135793810705
1106	42-43	0.25302474054516466
1106	44-45	0.23662964404955744
1106	46-47	0.2509597677692952
1106	48-49	0.14816537938003194
1106	50-51	0.14754347358165631
1106	52-53	0.07904242571751041
1106	54-55	0.10281570542714746
1106	56-57	0.16181188821954606
1106	58-59	0.16687220111774792
1106	60-61	0.1447350806002703
1106	62-63	0.13711237075446547
1106	64-65	0.178928140166569
1106	66-67	0.1812832307964527
1106	68-69	0.2261787345388555
1106	70-71	0.19627526218935998
1106	72-73	0.18609493871845473
1106	74-75	0.1644645938038778
1106	76-77	0.20270601260025956
1106	78-79	0.18865417119402395
1106	80-81	0.19269230666184
1106	82-83	0.19331048955329422
1106	84-85	0.20297366199804756
1106	86-87	0.18530147480352355
1106	88-89	0.12673539625644636
1106	90-91	0.12383920085865086
1106	92-93	0.16976597080382305
1106	94-95	0.2082035139209637
1106	96-97	0.20113064558720595
1106	98-99	0.20312772876561525
1106	100	0.26142215748417996
1107	1	0.09550807084678326
1107	2	0.16149457495927777
1107	3	0.08743621476023122
1107	4	0.1283209184197176
1107	5	0.11180700753772044
1107	6	0.14896237092743547
1107	7	0.10512330952518312
1107	8	0.08276795441742024
1107	9	0.16137168451574269
1107	10-11	0.16656846654523605
1107	12-13	0.11182291570476366
1107	14-15	0.20085700747423374
1107	16-17	0.21264672832204923
1107	18-19	0.18315591279986165
1107	20-21	0.19489659166845996
1107	22-23	0.2005800825498838
1107	24-25	0.22370552940800792
1107	26-27	0.2145904753932939
1107	28-29	0.22053781115781845
1107	30-31	0.20517278616426893
1107	32-33	0.3004536398094473
1107	34-35	0.3194852395692038
1107	36-37	0.3201412506698773
1107	38-39	0.30478962600520276
1107	40-41	0.2910396448880803
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2216	78-79	-0.771137886762773
2216	80-81	-0.8724188202530243
2216	82-83	-0.9098908029323951
2216	84-85	-0.990371099898006
2216	86-87	-0.9846085243417342
2216	88-89	-1.0071218523807879
2216	90-91	-1.011597507831624
2216	92-93	-0.9471704980717526
2216	94-95	-1.0903259888495391
2216	96-97	-1.3526135717209407
2216	98-99	-1.3281786781519251
2216	100	-1.2743510650415821
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128.0
3	335.0
4	256.0
5	333.0
6	800.0
7	1828.0
8	3397.0
9	5067.0
10	7441.0
11	10607.0
12	14991.0
13	21409.0
14	20915.0
15	24418.0
16	30484.0
17	43755.0
18	47299.0
19	25902.0
20	17147.0
21	15229.0
22	15393.0
23	16582.0
24	18399.0
25	22383.0
26	27813.0
27	34946.0
28	44211.0
29	55142.0
30	69398.0
31	86746.0
32	107957.0
33	137152.0
34	185849.0
35	279748.0
36	429492.0
37	838005.0
38	1210213.0
39	566470.0
40	4258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94842537242837	20.605795782673916	14.14630905134939	24.29946979354833
2	14.957209733316704	21.65263587772614	38.88087929979482	24.509275089162337
3	15.737696281919696	27.171075188956774	31.149585232769212	25.94164329635432
4	11.223804754264478	18.60911698857234	37.572062129213734	32.59501612794945
5	11.049625740734362	36.88221667930821	34.12354754602228	17.944610033935152
6	27.759935054789644	38.747670477800256	18.642008438734972	14.85038602867513
7	24.602523515848404	32.94307973753562	23.453667778953953	19.000728967662024
8	24.905749749318872	32.72803652852902	22.37356643488887	19.99264728726324
9	24.511458840342573	15.51041919467759	23.608106264484235	36.3700157004956
10-11	22.58684631177369	26.484864593525025	29.31398007868423	21.61430901601705
12-13	24.913280313955884	24.43359797996262	29.454683560946243	21.198438145135256
14-15	22.2541355069387	26.03491795624303	28.58002367456434	23.13092286225393
16-17	19.641704964859617	28.96236023429624	28.650106328420872	22.745828472423273
18-19	19.31048394177444	25.93320467962119	33.466178196797856	21.290133181806517
20-21	21.559488759084516	25.67076056226415	32.683899990499555	20.085850688151776
22-23	20.689590350791487	26.35031241149617	32.78793209569423	20.17216514201812
24-25	20.239752356831293	25.820514985225834	33.29896721542141	20.64076544252146
26-27	20.020642076877948	25.367928304522074	33.598441477044275	21.012988141555706
28-29	20.312848950716454	25.576173915236044	33.246752281912556	20.86422485213495
30-31	21.004122258394233	26.62532025737661	32.415382260105275	19.95517522412388
32-33	19.770341184452082	25.889004317640662	33.54788898022881	20.79276551767844
34-35	19.760015633192708	25.161650679640896	33.46479188424774	21.61354180291866
36-37	21.310669895745367	26.444974819444983	31.847711731556306	20.396643553253345
38-39	20.957237160178487	26.31576518310955	32.262147033585556	20.464850623126406
40-41	20.268014543944936	26.038630088351727	32.599738599819425	21.093616767883912
42-43	20.17479578154141	26.107115744655555	32.30478912659969	21.41329934720335
44-45	21.064092421753045	26.391015732463913	31.733011428898187	20.811880416884854
46-47	20.73838996794263	27.514551550857796	30.65234420421711	21.09471427698246
48-49	20.67096703023331	26.621047767580002	31.12035521055591	21.587629991630784
50-51	20.795773721990134	26.20393000359312	30.98882937385239	22.01146690056436
52-53	20.877606689713296	26.659120286087067	30.469457139000976	21.993815885198657
54-55	21.245012916353875	27.678976966689213	29.998798923736608	21.0772111932203
56-57	20.456511610183583	27.002033821069897	30.254346396189963	22.287108172556554
58-59	20.202579548034798	26.224860911745306	31.013925542739983	22.558633997479912
60-61	21.303492079572926	27.09191860350587	29.91945337937284	21.68513593754837
62-63	20.990848598145988	26.919104588398547	30.189224879178468	21.900821934277
64-65	20.738724145752414	26.448791859693515	30.93353610181349	21.878947892740577
66-67	20.709368730854166	26.72622910997631	30.113962673121573	22.45043948604795
68-69	21.69915550007829	26.973590677507925	29.86359654104323	21.46365728137056
70-71	21.845662786331204	26.40004781754769	30.083777514225947	21.67051188189516
72-73	21.139578171313254	26.626849603480313	30.733213594729097	21.500358630477333
74-75	21.027447891263392	26.49454697150841	30.461980200092775	22.016024937135423
76-77	21.16591826286871	26.88199954163738	29.709991089394666	22.24209110609924
78-79	21.53031737040836	27.169533773567743	29.79143271896829	21.508716137055607
80-81	21.035388475827226	26.59855088973227	30.191496968187927	22.17456366625258
82-83	20.77316649896254	26.702046815848483	30.274578129092816	22.250208556096158
84-85	21.04177911455135	26.688980829593827	30.003573943196255	22.265666112658568
86-87	21.32479178945577	26.852687747444897	29.71295153558231	22.10956892751702
88-89	20.62915447405591	26.79648429567721	30.022751535492258	22.551609694774623
90-91	20.38760232675311	26.57773771482371	29.918246659423513	23.11641329899966
92-93	20.82641857704925	27.0236686437157	29.525588153419847	22.624324625815206
94-95	20.726331401576534	26.58785726281873	29.923357087443254	22.762454248161486
96-97	20.44334831197601	26.632799625383846	30.073516168829418	22.850335893810726
98-99	20.380693009457946	26.53241098968836	30.01952104438826	23.067374956465436
100	20.674835424526037	26.883260995393172	29.55645162198104	22.88545195809975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2269.0
1	1506.5
2	8440.5
3	13688.5
4	7238.0
5	3137.0
6	4464.5
7	11152.5
8	17719.0
9	17773.5
10	12034.5
11	6453.0
12	5479.0
13	5831.5
14	6385.0
15	6937.5
16	7476.0
17	8237.0
18	9059.0
19	10003.0
20	11352.5
21	13299.5
22	16406.0
23	21193.5
24	26561.0
25	32606.5
26	41086.0
27	51189.5
28	62683.5
29	74662.0
30	83550.0
31	91091.5
32	99302.0
33	106907.0
34	113719.0
35	119963.5
36	123629.0
37	126245.5
38	128898.0
39	129079.0
40	129482.5
41	129301.5
42	126807.5
43	125560.5
44	125191.0
45	124745.0
46	126039.5
47	129422.5
48	134462.5
49	139062.0
50	140916.0
51	136620.5
52	127976.0
53	120584.5
54	116390.0
55	113994.5
56	108729.0
57	101741.0
58	95956.5
59	88808.0
60	77300.5
61	64241.5
62	52577.0
63	41798.5
64	32358.0
65	24718.0
66	18490.0
67	13313.0
68	9753.5
69	7197.0
70	5207.0
71	3852.5
72	2977.5
73	2312.0
74	1780.5
75	1332.0
76	942.5
77	740.0
78	614.0
79	477.5
80	295.5
81	123.0
82	56.5
83	34.5
84	20.5
85	4.5
86	3.0
87	2.0
88	1.0
89	3.5
90	2.5
91	0.0
92	1.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047682319585006225
2	0.0
3	2.0261608888812847E-4
4	0.06798895427134977
5	0.004299963664181393
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.01068237046415744
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.17796671602995E-4
26-27	0.0
28-29	2.2512898765347607E-4
30-31	0.003557038004924922
32-33	1.0130804444406423E-4
34-35	0.0015083642172782896
36-37	0.003984783081466526
38-39	0.0014070561728342255
40-41	0.0011143884888847066
42-43	0.0019361092938198943
44-45	0.0
46-47	3.939757283935831E-4
48-49	5.740789185163639E-4
50-51	0.0015759029135743324
52-53	7.541821086391448E-4
54-55	0.001091875590119359
56-57	0.005819584330842356
58-59	0.008633696676510807
60-61	0.0013620303753035302
62-63	0.002690291402459039
64-65	0.001136901387650054
66-67	1.1256449382673803E-4
68-69	8.667466024658828E-4
70-71	3.151805827148665E-4
72-73	0.0
74-75	1.9135963950545464E-4
76-77	0.0
78-79	4.952837728376473E-4
80-81	0.0
82-83	0.0010693626913540113
84-85	5.740789185163639E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.9135963950545464E-4
94-95	0.0
96-97	4.0523217777625693E-4
98-99	5.628224691336902E-4
100	3.3769348148021406E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4441898.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.58813912960098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55843570324237	47.15091589635902
2	14.99547526658179	18.470868340654594
3	4.297098289678038	7.939508619547843
4	1.5722703096202457	3.8733281031292996
5	0.7608419077286582	2.342941863441182
6	0.4374041127207294	1.616334321006237
7	0.29773541714136065	1.2835879206298335
8	0.1902578259700403	0.9374100365070602
9	0.14066539713767684	0.7796988044672236
>10	0.681174588383755	7.459004608254108
>50	0.04432234007356205	1.8810357189335363
>100	0.022343217816274817	2.4226447348426285
>500	0.0011341546741410354	0.4659477035545549
>1k	5.853699001980924E-4	0.5625664753609494
>5k	1.0975685628704715E-4	0.4719862201858062
>10k+	1.463424750493962E-4	2.3422206331261934
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31518	0.7095615432862259	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25982	0.5849301357212615	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24385	0.5489770363930013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22064	0.4967245983586296	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9736	0.2191855823794243	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5888	0.1325559479303667	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5323	0.1198361601279453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.2512898765347604E-5	0.0
4	0.0	0.0	0.0	6.753869629604281E-5	0.0
5	0.0	0.0	0.0	9.005159506139042E-5	0.0
6	0.0	0.0	0.0	9.005159506139042E-5	0.0
7	0.0	0.0	0.0	9.005159506139042E-5	0.0
8	0.0	0.0	0.0	1.1256449382673803E-4	0.0
9	0.0	0.0	0.0	1.1256449382673803E-4	0.0
10-11	0.0	0.0	0.0	1.1256449382673803E-4	0.0
12-13	0.0	0.0	0.0	1.1256449382673803E-4	0.0
14-15	0.0	0.0	0.0	2.0261608888812847E-4	0.0
16-17	0.0	0.0	0.0	2.2512898765347607E-4	0.0
18-19	0.0	0.0	0.0	2.9266768394951886E-4	0.0
20-21	0.0	0.0	0.0	4.5025797530695214E-4	0.0
22-23	0.0	0.0	0.0	6.978998617257758E-4	0.0
24-25	0.0	0.0	0.0	0.0012382094320941184	0.0
26-27	0.0	0.0	0.0	0.001778519002462461	0.0
28-29	0.0	0.0	0.0	0.0033881912641848147	0.0
30-31	0.0	0.0	0.0	0.007913283916019684	0.0
32-33	0.0	0.0	0.0	0.01799906256289541	0.0
34-35	0.0	0.0	0.0	0.0326549596591367	0.0
36-37	0.0	0.0	0.0	0.0528940556491842	0.0
38-39	0.0	0.0	0.0	0.08617937647375064	0.0
40-41	0.0	0.0	0.0	0.13843181450812242	0.0
42-43	0.0	0.0	0.0	0.19409495670544438	0.0
44-45	0.0	0.0	0.0	0.24892512164844846	0.0
46-47	0.0	0.0	0.0	0.311781135001299	0.0
48-49	0.0	0.0	0.0	0.3898896372676725	0.0
50-51	0.0	0.0	0.0	0.4796598210944961	0.0
52-53	0.0	0.0	0.0	0.5740564056175985	0.0
54-55	0.0	0.0	0.0	0.676084862822154	0.0
56-57	0.0	0.0	0.0	0.7854187556760646	0.0
58-59	0.0	0.0	0.0	0.9080352588015304	0.0
60-61	0.0	0.0	0.0	1.0383399168553624	0.0
62-63	0.0	0.0	0.0	1.173687464232632	0.0
64-65	0.0	0.0	0.0	1.3296793397777256	0.0
66-67	0.0	0.0	0.0	1.5117861778906225	0.0
68-69	0.0	0.0	0.0	1.7007256807788023	0.0
70-71	0.0	0.0	0.0	1.91188541474838	0.0
72-73	0.0	0.0	0.0	2.168408639730133	0.0
74-75	0.0	0.0	0.0	2.4174125565242606	0.0
76-77	0.0	0.0	0.0	2.6596626036887834	0.0
78-79	0.0	0.0	0.0	2.9347927395000966	0.0
80-81	0.0	0.0	0.0	3.2421726027927704	0.0
82-83	0.0	0.0	0.0	3.5743053982779434	0.0
84-85	0.0	0.0	0.0	3.905965422889044	0.0
86-87	0.0	0.0	0.0	4.259001444877843	0.0
88	0.0	0.0	0.0	4.547628063498982	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11505	0.0	60.07123	1
TATAACG	475	0.0	45.545334	2
GTATCAA	21750	0.0	43.888844	1
ATAACGC	490	0.0	41.27167	3
GTGGTAT	4045	0.0	32.915405	1
TGGTATC	4015	0.0	32.564087	2
TAACGCA	685	0.0	31.58253	4
ATCAACG	30355	0.0	31.203901	3
TATCAAC	30375	0.0	31.136904	2
TATCACG	560	0.0	31.073727	2
TCAACGC	30580	0.0	31.005068	4
TCTGTCG	1775	0.0	30.982937	8
CAACGCA	31840	0.0	29.806643	5
GTCTCGC	1725	0.0	29.72821	1
AACGCAG	32980	0.0	28.875128	6
TACCTGG	3570	0.0	27.928478	2
GTACCTG	3580	0.0	27.72878	1
CGCTCTG	1785	0.0	27.387028	5
TCGCTCT	2065	0.0	26.419073	4
ACGCAGA	36885	0.0	25.71618	7
>>END_MODULE