FastQCFastQC Report
Wed 25 May 2016
SRR2431428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7103232
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125270.17635634032508019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG9150.051.3724562
TAACGCA10250.044.4835364
GGTATCA60150.033.1376271
TACCTGG63850.032.5396462
ATAACGC14500.032.0936163
GTACCTG72650.030.2184661
GTATCAA86600.029.6934491
GTATAAC22900.026.6869561
ATCAACG91850.026.509533
TCAACGC91950.026.2250944
GTACATG204750.023.9929451
CAACGCA101050.023.9095885
TATCACG10500.023.7267362
ACCTGGG87350.023.3010983
TATCAAC106200.023.0160222
AACGCAG110400.022.5217086
TACATGG216450.021.4778162
ACATGGG217600.020.8890673
ACGCAGA125300.019.7308757
CGCAGAG130850.018.893468
Produced by FastQC (version 0.11.5)