##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431428_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7103232
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.121607178253505	31.0	31.0	34.0	26.0	34.0
2	30.144724683073846	31.0	31.0	34.0	26.0	34.0
3	30.249969591307167	31.0	31.0	34.0	26.0	34.0
4	33.704431588324866	37.0	35.0	37.0	28.0	37.0
5	33.774527848731395	37.0	35.0	37.0	28.0	37.0
6	33.89144758329729	37.0	35.0	37.0	30.0	37.0
7	33.79276616616211	37.0	35.0	37.0	29.0	37.0
8	33.72075739606984	37.0	35.0	37.0	29.0	37.0
9	35.19381444953508	39.0	35.0	39.0	28.0	39.0
10-11	35.106316885046134	39.0	35.0	39.0	27.5	39.0
12-13	35.08120648459743	39.0	35.0	39.0	27.0	39.0
14-15	36.26244996643781	40.0	37.0	41.0	27.0	41.0
16-17	36.23850241692796	40.0	36.5	41.0	27.0	41.0
18-19	36.19026838768606	40.0	36.5	41.0	27.0	41.0
20-21	36.26085907091307	40.0	37.0	41.0	27.0	41.0
22-23	36.18844893423163	40.0	36.5	41.0	27.0	41.0
24-25	36.17721820151728	40.0	36.0	41.0	27.0	41.0
26-27	36.13185906077684	40.0	36.0	41.0	27.0	41.0
28-29	35.99446357658035	40.0	36.0	41.0	27.0	41.0
30-31	35.97073529345515	40.0	36.0	41.0	27.0	41.0
32-33	35.93130253946373	40.0	36.0	41.0	27.0	41.0
34-35	35.83696323025913	40.0	36.0	41.0	27.0	41.0
36-37	35.71490773495783	40.0	35.5	41.0	26.5	41.0
38-39	35.62018726123544	40.0	35.0	41.0	26.0	41.0
40-41	35.52974638868616	40.0	35.0	41.0	26.0	41.0
42-43	35.30193854008992	40.0	35.0	41.0	25.0	41.0
44-45	35.15878891467997	39.0	35.0	41.0	25.0	41.0
46-47	35.01417720834685	39.0	35.0	41.0	24.5	41.0
48-49	34.86955578812574	39.0	34.5	41.0	24.0	41.0
50-51	34.68220860588532	39.0	34.0	41.0	24.0	41.0
52-53	34.57314142350975	39.0	34.0	40.5	24.0	41.0
54-55	34.4779186432317	38.5	34.0	41.0	23.0	41.0
56-57	34.29463221812268	38.0	34.0	40.0	23.0	41.0
58-59	34.06067048070512	38.0	33.5	40.0	22.5	41.0
60-61	33.81473771376184	37.5	33.0	40.0	22.0	41.0
62-63	33.51820417804177	37.0	33.0	40.0	21.5	41.0
64-65	33.35612577485854	37.0	33.0	40.0	20.5	41.0
66-67	33.0746208908846	36.0	33.0	39.5	20.0	41.0
68-69	32.791993560114605	36.0	33.0	39.0	19.5	41.0
70-71	32.48237344634104	35.0	32.0	39.0	18.5	41.0
72-73	32.16639545209843	35.0	32.0	38.5	17.5	40.0
74-75	31.820654245841894	35.0	32.0	37.0	14.0	40.0
76-77	31.505452729123867	35.0	32.0	37.0	12.5	39.0
78-79	31.186291113678955	35.0	31.5	36.5	8.0	39.0
80-81	30.901537778858977	35.0	31.0	36.0	7.0	38.5
82-83	30.623329563218547	35.0	31.0	36.0	3.5	37.0
84-85	30.35958497483962	35.0	31.0	35.0	2.0	37.0
86-87	30.131079767632535	35.0	31.0	35.0	2.0	36.5
88-89	29.892428826765055	35.0	31.0	35.0	2.0	36.0
90-91	29.70069434589775	34.0	31.0	35.0	2.0	36.0
92-93	29.529434206851192	34.0	30.5	35.0	2.0	36.0
94-95	29.32403890229124	34.0	30.0	35.0	2.0	35.0
96-97	29.252233912675244	34.0	30.5	35.0	2.0	35.0
98-99	29.111134128802213	34.0	30.0	35.0	2.0	35.0
100	28.98249585540779	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15968254742585586
1101	2	0.23891659723617664
1101	3	0.22573895757216533
1101	4	0.2825831090732436
1101	5	0.23059385176104996
1101	6	0.33688290080205263
1101	7	0.42354378707435103
1101	8	0.45103231851514636
1101	9	0.5785868510115435
1101	10-11	0.624591238682072
1101	12-13	0.5900595067650016
1101	14-15	0.7126125690242091
1101	16-17	0.6942883948034577
1101	18-19	0.6575524614099493
1101	20-21	0.6098742342474424
1101	22-23	0.6357299674526615
1101	24-25	0.6416112574495827
1101	26-27	0.6652180060687201
1101	28-29	0.7401759343841192
1101	30-31	0.728501566868438
1101	32-33	0.7251555413193813
1101	34-35	0.6513297420135729
1101	36-37	0.6645959217150263
1101	38-39	0.6744091315082201
1101	40-41	0.6508921894847646
1101	42-43	0.6646419743137457
1101	44-45	0.605838856076808
1101	46-47	0.587741078623111
1101	48-49	0.648543421885563
1101	50-51	0.5209273724881882
1101	52-53	0.5163752170700775
1101	54-55	0.6394578471631789
1101	56-57	0.6306422683549044
1101	58-59	0.661779619277425
1101	60-61	0.6489856246237267
1101	62-63	0.6426727853362593
1101	64-65	0.6505510434057626
1101	66-67	0.636239154676602
1101	68-69	0.6029695811486206
1101	70-71	0.5989129513809246
1101	72-73	0.5925516166588096
1101	74-75	0.6093151024368453
1101	76-77	0.5498167658516238
1101	78-79	0.5452023634146457
1101	80-81	0.5548403609785701
1101	82-83	0.5487799180012622
1101	84-85	0.5627906610301849
1101	86-87	0.5845150238788115
1101	88-89	0.5310440297433523
1101	90-91	0.5510249443057056
1101	92-93	0.5334073666885963
1101	94-95	0.5649551061158036
1101	96-97	0.6077689987582282
1101	98-99	0.5995174332925934
1101	100	0.5831975882259854
1102	1	0.15367587922575865
1102	2	0.23175482982594175
1102	3	0.240166822951565
1102	4	0.29339093251876847
1102	5	0.22175630711328154
1102	6	0.3070998008356156
1102	7	0.3404874932595163
1102	8	0.3598254168217352
1102	9	0.552570404068085
1102	10-11	0.5293769621432176
1102	12-13	0.5628723034121847
1102	14-15	0.6639049565883823
1102	16-17	0.6551840067027968
1102	18-19	0.5662044926162793
1102	20-21	0.5968290000810796
1102	22-23	0.594048401316023
1102	24-25	0.6315965899437614
1102	26-27	0.6425007655519934
1102	28-29	0.6982378673799019
1102	30-31	0.6288187134907091
1102	32-33	0.6883896808126622
1102	34-35	0.6984264449272715
1102	36-37	0.6147299659217609
1102	38-39	0.5218046249581079
1102	40-41	0.5882551680494288
1102	42-43	0.6377738397024046
1102	44-45	0.512295071709211
1102	46-47	0.49321492685105284
1102	48-49	0.4734271527122331
1102	50-51	0.44720014971496624
1102	52-53	0.44871617944988174
1102	54-55	0.49914349568604877
1102	56-57	0.4899307296783775
1102	58-59	0.49219457557023105
1102	60-61	0.45124686535806546
1102	62-63	0.43729244470443973
1102	64-65	0.4873660778749098
1102	66-67	0.5022433512324511
1102	68-69	0.474478257993745
1102	70-71	0.4338661956028602
1102	72-73	0.45649261168112787
1102	74-75	0.49325573253067034
1102	76-77	0.4964198679448799
1102	78-79	0.4360935410367688
1102	80-81	0.44504938428339713
1102	82-83	0.4232963306298707
1102	84-85	0.3934886805985762
1102	86-87	0.39471659691557903
1102	88-89	0.4948502814680431
1102	90-91	0.424615328031674
1102	92-93	0.42079548967949165
1102	94-95	0.4566371855283293
1102	96-97	0.4319663563530298
1102	98-99	0.47838089669734885
1102	100	0.4507399066045288
1103	1	0.17125746024490596
1103	2	0.2453366525176648
1103	3	0.2587682693694582
1103	4	0.23985357979763933
1103	5	0.2325319810942048
1103	6	0.3935065139346108
1103	7	0.4282978941965325
1103	8	0.4240889889078616
1103	9	0.4906085297575302
1103	10-11	0.5933943693764334
1103	12-13	0.493825892105491
1103	14-15	0.622448630357475
1103	16-17	0.5429080916231968
1103	18-19	0.46641031208083916
1103	20-21	0.5283933008381325
1103	22-23	0.5112381955060528
1103	24-25	0.5550835112263002
1103	26-27	0.5678356004137513
1103	28-29	0.6089820815757392
1103	30-31	0.475697139716992
1103	32-33	0.5290180944229661
1103	34-35	0.521778074489518
1103	36-37	0.5442383577506646
1103	38-39	0.5483698477201813
1103	40-41	0.5501636307810855
1103	42-43	0.5227408446275632
1103	44-45	0.47317464491053585
1103	46-47	0.39593400654479183
1103	48-49	0.3772082203137046
1103	50-51	0.3598880401496558
1103	52-53	0.4230026684765207
1103	54-55	0.4930858049272828
1103	56-57	0.4225029180805606
1103	58-59	0.4134789259964222
1103	60-61	0.428293452318691
1103	62-63	0.4132606875188145
1103	64-65	0.41569611304645804
1103	66-67	0.4291464851748259
1103	68-69	0.33541592997914904
1103	70-71	0.3503406987304132
1103	72-73	0.3804336239013395
1103	74-75	0.3758217494954792
1103	76-77	0.35562886461620025
1103	78-79	0.33536535793365374
1103	80-81	0.3584890482073213
1103	82-83	0.43785483251162205
1103	84-85	0.36679993508783326
1103	86-87	0.2949686254750574
1103	88-89	0.31218877692128544
1103	90-91	0.3149801291451553
1103	92-93	0.3301912344214806
1103	94-95	0.3225764873985497
1103	96-97	0.24398434948194847
1103	98-99	0.24304728246812957
1103	100	0.26262924154146816
1104	1	0.13349959481661955
1104	2	0.2115230119447027
1104	3	0.17613652343360897
1104	4	0.25257975108587516
1104	5	0.19540498730719946
1104	6	0.3100186365944424
1104	7	0.4029812750766908
1104	8	0.3330443704468209
1104	9	0.4613697740341678
1104	10-11	0.5325300183717943
1104	12-13	0.46053034417734295
1104	14-15	0.544664832398027
1104	16-17	0.5660606633364225
1104	18-19	0.46991521546109993
1104	20-21	0.4511381682537845
1104	22-23	0.4615955278147652
1104	24-25	0.5166936485794409
1104	26-27	0.5630033287467242
1104	28-29	0.6175518807400451
1104	30-31	0.578951010500333
1104	32-33	0.6157674714592289
1104	34-35	0.556639843977834
1104	36-37	0.5374033714220943
1104	38-39	0.5906066449390579
1104	40-41	0.5588555358408556
1104	42-43	0.5228820276150685
1104	44-45	0.5024197259934695
1104	46-47	0.5453975390911836
1104	48-49	0.5114236727390207
1104	50-51	0.46430803305756285
1104	52-53	0.4664811025118212
1104	54-55	0.5455758849356229
1104	56-57	0.5172576653711403
1104	58-59	0.5391108895438848
1104	60-61	0.5151926177175596
1104	62-63	0.5040452687646138
1104	64-65	0.5626310265876953
1104	66-67	0.5590700777173936
1104	68-69	0.5449504402751018
1104	70-71	0.4873774972191782
1104	72-73	0.5076667307659619
1104	74-75	0.5423363349569144
1104	76-77	0.49148722216227014
1104	78-79	0.5242543467669663
1104	80-81	0.5214561074663706
1104	82-83	0.5051889969763543
1104	84-85	0.5014928006402215
1104	86-87	0.4757067559739099
1104	88-89	0.4921570798951329
1104	90-91	0.42136834306178983
1104	92-93	0.3909494492597432
1104	94-95	0.43292881617948353
1104	96-97	0.49759279154251956
1104	98-99	0.5162062997949022
1104	100	0.47463908389715215
1105	1	-0.01717216460682991
1105	2	0.007432786134458524
1105	3	0.03530581573329172
1105	4	-0.03673928020076289
1105	5	-0.009076779249632239
1105	6	0.08741152446564371
1105	7	0.13580151984873368
1105	8	0.10131899745974948
1105	9	0.14440462764721929
1105	10-11	0.21479083314446257
1105	12-13	0.18740097530744038
1105	14-15	0.33372502539770466
1105	16-17	0.24923376456527535
1105	18-19	0.21227514825267235
1105	20-21	0.20707484162893053
1105	22-23	0.21280372696362804
1105	24-25	0.20664463467107197
1105	26-27	0.2653996505665006
1105	28-29	0.32680476462136454
1105	30-31	0.28956806869990004
1105	32-33	0.2924939379435614
1105	34-35	0.2722731172872841
1105	36-37	0.24594150915154245
1105	38-39	0.2381123696773173
1105	40-41	0.2429552053627333
1105	42-43	0.2541868584063707
1105	44-45	0.1916331121699102
1105	46-47	0.153850649523676
1105	48-49	0.09386946883750369
1105	50-51	0.1869833016516509
1105	52-53	0.13886935514084797
1105	54-55	0.1813155132105706
1105	56-57	0.19938353231958672
1105	58-59	0.19405746069326568
1105	60-61	0.16259895329854857
1105	62-63	0.20380043929323222
1105	64-65	0.263008346691862
1105	66-67	0.2619579696288312
1105	68-69	0.257154622904487
1105	70-71	0.249974298558449
1105	72-73	0.21696983173214335
1105	74-75	0.23493577801234267
1105	76-77	0.21023523040148717
1105	78-79	0.22199309986752525
1105	80-81	0.23514800183199114
1105	82-83	0.23936553610788636
1105	84-85	0.19314520822181436
1105	86-87	0.15674567816878238
1105	88-89	0.17744546520308901
1105	90-91	0.19612550240914928
1105	92-93	0.13117297648180326
1105	94-95	0.1441437156469334
1105	96-97	0.18637952549543257
1105	98-99	0.20959863428022985
1105	100	0.19403648264462348
1106	1	0.16960954232674652
1106	2	0.22668298241068285
1106	3	0.15870817905059909
1106	4	0.2503121261061523
1106	5	0.24317072008756924
1106	6	0.29578186542774887
1106	7	0.3409834219378425
1106	8	0.33038081652386353
1106	9	0.40431251236809373
1106	10-11	0.4785394700530574
1106	12-13	0.416814522243385
1106	14-15	0.4972006254757062
1106	16-17	0.4001203117547334
1106	18-19	0.40250365975604296
1106	20-21	0.4897031322546326
1106	22-23	0.47832153081171214
1106	24-25	0.43659758673681637
1106	26-27	0.45060910053840786
1106	28-29	0.5670562252467661
1106	30-31	0.5598817028711451
1106	32-33	0.5407690652625377
1106	34-35	0.5056363442677636
1106	36-37	0.5005898712816261
1106	38-39	0.42581692457514464
1106	40-41	0.4826681408163225
1106	42-43	0.46456342607888956
1106	44-45	0.4348237236728707
1106	46-47	0.3548113019422203
1106	48-49	0.3839987767037698
1106	50-51	0.36705397933037176
1106	52-53	0.4145044281180006
1106	54-55	0.48782639716019816
1106	56-57	0.47263857881483773
1106	58-59	0.4752164266454244
1106	60-61	0.4728556679570559
1106	62-63	0.5147314206225673
1106	64-65	0.5407842525958415
1106	66-67	0.5102655596467542
1106	68-69	0.5198780996347452
1106	70-71	0.49828209374792465
1106	72-73	0.4864885127661225
1106	74-75	0.5366613318629163
1106	76-77	0.5221446703044919
1106	78-79	0.4860453314017761
1106	80-81	0.46140113176961606
1106	82-83	0.4240512742940652
1106	84-85	0.4583178177721656
1106	86-87	0.4974245740569465
1106	88-89	0.5120017410516304
1106	90-91	0.5212337331386223
1106	92-93	0.5132037838384242
1106	94-95	0.5519446672526556
1106	96-97	0.5563000591792857
1106	98-99	0.5616323986604499
1106	100	0.5272198918054123
1107	1	0.08208121247560385
1107	2	0.05768596727480357
1107	3	0.12792860433994946
1107	4	0.14113568228174245
1107	5	0.13564489642809008
1107	6	0.202431985449266
1107	7	0.19521086494661688
1107	8	0.2535276222506653
1107	9	0.275739111810239
1107	10-11	0.33039691334214893
1107	12-13	0.2786253403442345
1107	14-15	0.32505093534965823
1107	16-17	0.329943762863266
1107	18-19	0.3589922237054566
1107	20-21	0.33292068141442854
1107	22-23	0.3622119606534966
1107	24-25	0.385880337809958
1107	26-27	0.38370465217995076
1107	28-29	0.4332328098163529
1107	30-31	0.42370993671629265
1107	32-33	0.43544882201079815
1107	34-35	0.40358105346385287
1107	36-37	0.35177799831746626
1107	38-39	0.3587398895720355
1107	40-41	0.3536292334503699
1107	42-43	0.37235950076888713
1107	44-45	0.3833675692574232
1107	46-47	0.3702164625766571
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2216	74-75	-1.439637524166013
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2216	80-81	-1.2342052984720837
2216	82-83	-1.3518025632530204
2216	84-85	-1.2979243774497462
2216	86-87	-1.3785787873523887
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2216	90-91	-1.4567169562817703
2216	92-93	-1.3606272448345713
2216	94-95	-1.2986059047748029
2216	96-97	-1.3678403275475333
2216	98-99	-1.6740608803205994
2216	100	-1.620249325571347
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	227778.0
3	37894.0
4	24657.0
5	19695.0
6	16390.0
7	15929.0
8	16997.0
9	18711.0
10	20183.0
11	21418.0
12	22175.0
13	23126.0
14	23963.0
15	24362.0
16	24814.0
17	24985.0
18	24975.0
19	24763.0
20	25412.0
21	26908.0
22	30557.0
23	39531.0
24	52323.0
25	65299.0
26	75251.0
27	78227.0
28	89255.0
29	107568.0
30	127780.0
31	152740.0
32	191757.0
33	248704.0
34	340702.0
35	503672.0
36	833626.0
37	1393917.0
38	1577338.0
39	528362.0
40	1488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.971071302354595	19.133307707795655	12.933377826025128	23.962243163824624
2	16.729692092670817	20.211091883651388	38.174894043650234	24.884321980027565
3	18.333901202098392	25.508815106623228	29.47193836605853	26.68534532521985
4	13.094535807943016	17.514595273139204	36.73469675087102	32.656172168046766
5	13.921101242939127	36.90715408052353	33.541982282344854	15.629762394192484
6	31.299255253318766	36.67204746554517	18.02289784531036	14.005799435825702
7	27.71163386096721	31.918334406660392	21.916709632601133	18.453322099771263
8	25.74490108533558	35.13797874832739	20.20860271491582	18.908517451421208
9	25.597787231981627	17.4741066101179	20.516451450404336	36.41165470749614
10-11	24.60119684941815	26.551564414953788	27.96495473977571	20.88228399585235
12-13	24.892482012286766	24.454469491810585	27.717100312343153	22.9359481835595
14-15	22.677418812110567	26.21739332745905	24.77304954104467	26.332138319385717
16-17	22.74216007443168	28.915858820554647	25.235300366139725	23.106680738873948
18-19	22.813620923231575	28.215916982298232	26.588662464815922	22.381799629654267
20-21	23.926742185542164	27.529994959776765	26.086759418037243	22.456503436643832
22-23	23.59456019642875	27.593966067046516	25.930488270168368	22.880985466356368
24-25	23.284370989940008	27.547123935434552	26.164782408500148	23.003722666125288
26-27	23.30906592536329	27.376986979087842	26.219021921621106	23.094925173927766
28-29	23.22072748183351	27.514211313630582	26.265022342725125	23.000038861810783
30-31	23.36333677842883	27.8117899418135	25.948509971559307	22.876363308198357
32-33	23.07752467632409	27.60902965720433	26.23054287561026	23.08290279086132
34-35	23.28699371544142	27.202986729829732	26.266761585837195	23.243257968891655
36-37	23.436358498798285	27.454541282033485	26.174975850947018	22.934124368221212
38-39	23.314803391353177	27.622002980925135	26.089887993212844	22.973305634508844
40-41	23.28081685520601	27.30765819354203	26.27513603694292	23.136388914309045
42-43	23.126901692326665	27.42128408685824	26.304709467309333	23.147104753505758
44-45	23.24137298464459	27.526503306485512	26.198918207678812	23.03320550119109
46-47	23.20231671967744	27.448162531361746	26.191081082584105	23.158439666376708
48-49	23.13613435700603	27.2557113828106	26.400162804758253	23.207991455425123
50-51	23.066059783526867	27.230112516472005	26.371449367587594	23.332378332413526
52-53	23.09926464247715	27.246170020234388	26.33430275837383	23.320262578914637
54-55	23.25602055041573	27.5201248162376	26.168216404460026	23.055638228886647
56-57	22.873069661820377	27.360042122868688	26.281291223604576	23.485596991706355
58-59	23.04241628768102	27.067870663938358	26.414553780788747	23.47515926759187
60-61	23.34714912110894	27.381600834015558	26.1391302389066	23.132119805968898
62-63	23.184182641533553	27.387916651913525	26.257240507945472	23.170660198607447
64-65	23.22352574633167	27.12001232731673	26.370363362302385	23.28609856404922
66-67	22.993390758296293	27.264419013436346	26.284693309884116	23.457496918383246
68-69	23.294771373920355	27.173556411392987	26.302535223046032	23.229136991640626
70-71	23.272676742669137	27.00291557667499	26.393423628123962	23.330984052531907
72-73	23.10648626686215	27.224069475624376	26.526304660062355	23.143139597451118
74-75	23.121113689421772	27.094388578439638	26.449581747015284	23.33491598512331
76-77	23.198293972908125	27.067764176035585	26.22435213593029	23.509589715125998
78-79	23.153304751483265	27.31062999151414	26.359347838252678	23.176717418749917
80-81	23.022236175731148	27.062548989311136	26.52309022198867	23.39212461296904
82-83	23.0746521352099	27.032575408070734	26.631899310711255	23.260873146008112
84-85	23.12082561042886	27.077036310902365	26.31904603924548	23.48309203942329
86-87	23.324698336487252	27.142131400479357	26.29180207463771	23.24136818839568
88-89	23.125711572214364	27.0770235962872	26.40104495087996	23.396219880618474
90-91	23.05174437201256	27.005139466416782	26.363332653815135	23.57978350775552
92-93	23.17271590632162	27.149152007392864	26.34144480364755	23.336687282637968
94-95	23.168778336633416	26.9370450802054	26.408938117427915	23.485238465733268
96-97	23.05453045987748	27.048543804133697	26.389587915526263	23.507337820462553
98-99	23.00825484066475	27.07707404015568	26.475287919860396	23.43938319931917
100	23.163394080963553	26.93065005332922	26.44937778823964	23.456578077467586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9022.0
1	7536.0
2	5987.0
3	5079.0
4	3625.0
5	2718.0
6	2365.0
7	2441.5
8	2566.5
9	2475.0
10	2207.0
11	1949.5
12	1895.5
13	1927.5
14	2032.5
15	2162.5
16	2370.0
17	2876.0
18	3592.0
19	4635.5
20	6375.5
21	8546.0
22	11371.5
23	15605.0
24	21926.5
25	28826.0
26	37047.5
27	47495.0
28	60164.0
29	75352.5
30	88468.5
31	100516.5
32	113151.0
33	124602.5
34	135188.0
35	145852.5
36	157484.0
37	169095.5
38	179828.5
39	188684.5
40	197917.5
41	207216.0
42	213103.0
43	218457.5
44	222711.0
45	226122.5
46	231321.0
47	233251.0
48	233619.0
49	234967.0
50	237117.5
51	239306.5
52	239266.5
53	239827.5
54	239021.5
55	231401.0
56	217622.0
57	200071.5
58	185100.0
59	174871.5
60	160746.5
61	141113.0
62	120895.0
63	100550.5
64	80627.5
65	64407.0
66	51152.5
67	40060.0
68	31826.0
69	25276.0
70	19188.0
71	14491.0
72	11554.0
73	9457.0
74	7667.5
75	5819.5
76	4246.0
77	3056.5
78	2287.5
79	1738.0
80	1097.5
81	686.0
82	498.0
83	373.0
84	278.5
85	227.0
86	186.0
87	147.0
88	119.0
89	103.5
90	80.0
91	71.5
92	61.0
93	41.5
94	35.5
95	33.5
96	32.0
97	26.5
98	28.0
99	33.0
100	54.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013261568818250623
2	0.0076444075035138935
3	0.009178920243629943
4	0.008306078134573108
5	0.010023606155620427
6	0.011501806501603777
7	0.009939137564421378
8	0.007658485602047068
9	0.00639145673406134
10-11	0.005870567088333874
12-13	0.006574472014992612
14-15	0.007398040779183335
16-17	0.004821748747612355
18-19	0.005764981349335063
20-21	0.00523705265434101
22-23	0.005659395610336252
24-25	0.005525653674271092
26-27	0.006011348073665622
28-29	0.016105344721951922
30-31	0.005363755541139582
32-33	0.0052229745558078355
34-35	0.004878061141745054
36-37	0.006602628212058962
38-39	0.0056805127581360144
40-41	0.005377833639672757
42-43	0.004899178289544816
44-45	0.0041882343136194905
46-47	0.006060621418531733
48-49	0.003456173189894403
50-51	0.003829242801023534
52-53	0.003160533120697733
54-55	0.004364210545284175
56-57	0.002843775903701301
58-59	0.0024003157999062962
60-61	0.0034772903376941652
62-63	0.0031746112192309077
64-65	0.004329015298951238
66-67	0.0031464550221645586
68-69	0.003484329386960753
70-71	0.00491325638807799
72-73	0.002731151115435903
74-75	0.002456628194038995
76-77	0.002527018686704869
78-79	0.0033717045986953547
80-81	0.003934828540022345
82-83	0.0015908251342487477
84-85	0.0024284719969726457
86-87	0.0022595348145745485
88-89	0.0017316061195804951
90-91	0.0022524957653079613
92-93	0.0022947300609074857
94-95	0.001766801365913432
96-97	0.0014218879518506505
98-99	0.0017879185137131943
100	0.0025903701301041556
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7103232.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.31971809435742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74726837810937	41.34997815000287
2	14.430036977516242	15.965311553747036
3	4.63610425465988	7.6940394127150675
4	2.02501173736719	4.480923137956715
5	1.089256657460823	3.012868561156738
6	0.6799306905890086	2.256814447625143
7	0.45443425481748084	1.7597422408245695
8	0.3184726354216488	1.409425312983414
9	0.2248906169462348	1.1196796978378645
>10	1.2584559406376714	12.60146887246335
>50	0.0905230212270274	3.4281568711209993
>100	0.044238199165586876	4.191680811419078
>500	0.00119820350880819	0.44113247123902954
>1k	1.5294220627160967E-4	0.11213273807491134
>5k	0.0	0.0
>10k+	2.54903668391687E-5	0.17664572083314056
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12527	0.17635634032508019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.8156197066349515E-5	0.0	0.0	0.0
6	0.0	2.8156197066349515E-5	0.0	0.0	0.0
7	0.0	2.8156197066349515E-5	0.0	0.0	0.0
8	0.0	2.8156197066349515E-5	0.0	0.0	0.0
9	0.0	2.8156197066349515E-5	0.0	0.0	0.0
10-11	0.0	2.8156197066349515E-5	0.0	2.1117147799762136E-5	0.0
12-13	0.0	3.5195246332936895E-5	0.0	2.8156197066349515E-5	0.0
14-15	0.0	6.33514433992864E-5	0.0	2.8156197066349515E-5	0.0
16-17	0.0	8.446859119904854E-5	0.0	4.927334486611165E-5	0.0
18-19	0.0	9.150764046563593E-5	0.0	7.039049266587379E-5	0.0
20-21	0.0	1.0558573899881069E-4	0.0	9.150764046563593E-5	0.0
22-23	0.0	1.1262478826539806E-4	0.0	2.1117147799762137E-4	0.0
24-25	0.0	1.1966383753198544E-4	0.0	5.20889645727466E-4	0.0
26-27	0.0	1.2670288679857283E-4	0.0	8.587640105236602E-4	0.0
28-29	0.0	1.3374193606516022E-4	0.0	0.0018864652034454176	0.0
30-31	7.039049266587379E-6	1.4782003459833494E-4	0.0	0.004765436353479655	0.0
32-33	1.4078098533174758E-5	1.5485908386492233E-4	0.0	0.011903032309799257	0.0
34-35	1.4078098533174758E-5	1.5485908386492233E-4	0.0	0.02282763677154287	0.0
36-37	1.4078098533174758E-5	1.6189813313150972E-4	0.0	0.04014369796734782	0.0
38-39	1.4078098533174758E-5	1.7597623166468445E-4	0.0	0.06601924307132304	0.0
40-41	1.4078098533174758E-5	1.9005433019785923E-4	0.0	0.09787094100263091	0.0
42-43	1.4078098533174758E-5	1.9709337946444662E-4	0.0	0.13088408206292573	0.0
44-45	1.4078098533174758E-5	1.9709337946444662E-4	0.0	0.17041538274408044	0.0
46-47	1.4078098533174758E-5	2.04132428731034E-4	0.0	0.2171757870220204	0.0
48-49	1.4078098533174758E-5	2.1821052726420873E-4	0.0	0.2688916819836379	0.0
50-51	1.4078098533174758E-5	2.2524957653079612E-4	0.0	0.3233035328143583	0.0
52-53	1.4078098533174758E-5	2.2524957653079612E-4	0.0	0.38030575377518294	0.0
54-55	1.4078098533174758E-5	2.2524957653079612E-4	0.0	0.44491014794392186	0.0
56-57	1.4078098533174758E-5	2.3228862579738351E-4	0.0	0.5203969122788049	0.0
58-59	1.4078098533174758E-5	2.4636672433055824E-4	0.0	0.6047951129851876	1.4078098533174758E-5
60-61	1.4078098533174758E-5	2.5340577359714566E-4	0.0	0.6948034359570404	1.4078098533174758E-5
62-63	2.8156197066349515E-5	2.5340577359714566E-4	0.0	0.7898869134501028	1.4078098533174758E-5
64-65	2.8156197066349515E-5	2.5340577359714566E-4	0.0	0.8942126626301943	1.4078098533174758E-5
66-67	2.8156197066349515E-5	2.5340577359714566E-4	0.0	1.0151646461779653	1.4078098533174758E-5
68-69	2.8156197066349515E-5	2.5340577359714566E-4	0.0	1.1470764294338127	1.4078098533174758E-5
70-71	2.8156197066349515E-5	2.5340577359714566E-4	0.0	1.2942840667459545	1.4078098533174758E-5
72-73	2.8156197066349515E-5	2.5340577359714566E-4	0.0	1.4568931438533896	1.4078098533174758E-5
74-75	2.8156197066349515E-5	2.5340577359714566E-4	0.0	1.6213394128194039	1.4078098533174758E-5
76-77	2.8156197066349515E-5	2.60444822863733E-4	0.0	1.8002157327819224	1.4078098533174758E-5
78-79	2.8156197066349515E-5	2.674838721303204E-4	0.0	2.000786402584063	1.4078098533174758E-5
80-81	2.8156197066349515E-5	2.674838721303204E-4	0.0	2.2245507397196094	1.4078098533174758E-5
82-83	2.8156197066349515E-5	2.674838721303204E-4	0.0	2.463470149926118	1.4078098533174758E-5
84-85	2.8156197066349515E-5	2.674838721303204E-4	0.0	2.7032201679460846	1.4078098533174758E-5
86-87	2.8156197066349515E-5	2.674838721303204E-4	0.0	2.9591599992792013	1.4078098533174758E-5
88	2.8156197066349515E-5	2.674838721303204E-4	0.0	3.172386879662666	1.4078098533174758E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	915	0.0	51.372456	2
TAACGCA	1025	0.0	44.483536	4
GGTATCA	6015	0.0	33.137627	1
TACCTGG	6385	0.0	32.539646	2
ATAACGC	1450	0.0	32.093616	3
GTACCTG	7265	0.0	30.218466	1
GTATCAA	8660	0.0	29.693449	1
GTATAAC	2290	0.0	26.686956	1
ATCAACG	9185	0.0	26.50953	3
TCAACGC	9195	0.0	26.225094	4
GTACATG	20475	0.0	23.992945	1
CAACGCA	10105	0.0	23.909588	5
TATCACG	1050	0.0	23.726736	2
ACCTGGG	8735	0.0	23.301098	3
TATCAAC	10620	0.0	23.016022	2
AACGCAG	11040	0.0	22.521708	6
TACATGG	21645	0.0	21.477816	2
ACATGGG	21760	0.0	20.889067	3
ACGCAGA	12530	0.0	19.730875	7
CGCAGAG	13085	0.0	18.89346	8
>>END_MODULE