FastQCFastQC Report
Wed 25 May 2016
SRR2431428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7103232
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177600.2500270299491837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157040.2210824593649764No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148860.20956657476483947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136420.19205342018957006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95900.047.835651
TATAACG9550.047.770822
TAACGCA11400.039.6059724
GTATCAA161900.036.9372371
ATAACGC14000.033.5943573
TACCTGG65450.030.612182
TATCACG10850.029.042862
ATCAACG205550.028.5556073
TCAACGC206800.028.428494
GTACCTG74000.027.2766651
CAACGCA217100.027.1742785
TATCAAC217700.026.9618992
AACGCAG230850.025.8811556
GTATAAC23100.025.6639941
ACCTGGG83550.023.4738273
ACGCAGA261650.022.7986357
GTACATG196300.022.3868141
CGCAGAG268350.022.369558
AGAGTAC278800.020.76393310-11
TACATGG211600.020.2709222
Produced by FastQC (version 0.11.5)