##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431428_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7103232
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26870697733088	33.0	31.0	34.0	30.0	34.0
2	32.414010270254444	34.0	31.0	34.0	31.0	34.0
3	32.52674697940318	34.0	31.0	34.0	31.0	34.0
4	36.06051400263993	37.0	35.0	37.0	35.0	37.0
5	35.95889167072116	37.0	35.0	37.0	35.0	37.0
6	35.975440616327894	37.0	35.0	37.0	35.0	37.0
7	35.91502558272065	37.0	35.0	37.0	35.0	37.0
8	35.89767136987783	37.0	35.0	37.0	35.0	37.0
9	37.60557715135871	39.0	37.0	39.0	35.0	39.0
10-11	37.5310782471979	39.0	37.0	39.0	34.5	39.0
12-13	37.508443973109706	39.0	37.0	39.0	34.0	39.0
14-15	38.887500366030565	40.0	38.0	41.0	35.0	41.0
16-17	38.93098634537067	40.0	38.0	41.0	35.0	41.0
18-19	38.96269979637438	40.0	38.5	41.0	35.0	41.0
20-21	38.963413626360506	40.0	38.5	41.0	35.0	41.0
22-23	38.91570435824143	40.0	38.0	41.0	35.0	41.0
24-25	38.866558983290986	40.0	38.0	41.0	35.0	41.0
26-27	38.74205129439669	40.0	38.0	41.0	34.0	41.0
28-29	38.65751414285779	40.0	38.0	41.0	34.0	41.0
30-31	38.53659946627113	40.0	38.0	41.0	34.0	41.0
32-33	38.414583460036226	40.0	38.0	41.0	34.0	41.0
34-35	38.30117980096948	40.0	38.0	41.0	34.0	41.0
36-37	38.18985632174199	40.0	38.0	41.0	33.5	41.0
38-39	38.11700863775813	40.0	38.0	41.0	33.0	41.0
40-41	38.02749227393953	40.0	38.0	41.0	33.0	41.0
42-43	37.946317042720835	40.0	37.0	41.0	33.0	41.0
44-45	37.796747241255815	40.0	37.0	41.0	33.0	41.0
46-47	37.59255850013064	40.0	37.0	41.0	33.0	41.0
48-49	37.44510984577161	40.0	37.0	41.0	32.0	41.0
50-51	37.33958640235881	40.0	36.5	41.0	32.0	41.0
52-53	37.197060507104375	40.0	36.0	41.0	32.0	41.0
54-55	37.02833344032688	40.0	36.0	41.0	31.5	41.0
56-57	36.78676368729052	39.0	35.0	41.0	31.0	41.0
58-59	36.59842906721897	39.0	35.0	41.0	31.0	41.0
60-61	36.36067264873229	39.0	35.0	41.0	31.0	41.0
62-63	36.099569463590655	38.0	35.0	41.0	31.0	41.0
64-65	35.831554495193174	37.5	35.0	40.0	30.0	41.0
66-67	35.541943371693336	37.0	35.0	40.0	30.0	41.0
68-69	35.23114710317782	36.5	35.0	39.5	30.0	41.0
70-71	34.88675183353155	36.0	34.5	39.0	29.5	41.0
72-73	34.5534032958518	35.5	34.0	39.0	29.0	41.0
74-75	34.239543633095465	35.0	34.0	38.5	29.0	40.0
76-77	33.89785332930137	35.0	34.0	37.0	29.0	39.5
78-79	33.609581384924496	35.0	34.0	37.0	29.0	39.0
80-81	33.32640719041698	35.0	34.0	36.0	29.0	39.0
82-83	33.10463279250909	35.0	34.0	36.0	29.0	37.5
84-85	32.915743847307816	35.0	34.0	36.0	29.0	37.0
86-87	32.705233687988795	35.0	34.0	35.0	28.5	37.0
88-89	32.59399601477186	35.0	34.0	35.0	29.0	36.0
90-91	32.44056057862111	35.0	34.0	35.0	29.0	36.0
92-93	32.27071789292536	35.0	33.0	35.0	27.5	36.0
94-95	32.13114523079072	35.0	33.0	35.0	27.0	36.0
96-97	31.98201044257037	35.0	33.0	35.0	27.0	35.0
98-99	31.82615230644304	35.0	33.0	35.0	27.0	35.0
100	31.72727090991819	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1629166242956117
1101	2	0.2398195266371772
1101	3	0.17904616472027612
1101	4	0.1641556592506035
1101	5	0.1527312908214853
1101	6	0.13680238396236177
1101	7	0.1717278992426401
1101	8	0.23860138799625474
1101	9	0.27277961918606053
1101	10-11	0.26129150836656123
1101	12-13	0.28840706003272487
1101	14-15	0.3940054212415376
1101	16-17	0.3731735050421463
1101	18-19	0.36970915941024174
1101	20-21	0.29594668725329143
1101	22-23	0.3134611728118486
1101	24-25	0.3158143190903644
1101	26-27	0.357569527498093
1101	28-29	0.3683421608971287
1101	30-31	0.3674829547207139
1101	32-33	0.3894212793128773
1101	34-35	0.43656896621263286
1101	36-37	0.3978123343488491
1101	38-39	0.3942377326864275
1101	40-41	0.46078113176749724
1101	42-43	0.5042631747079369
1101	44-45	0.5094975428048087
1101	46-47	0.49019716385337375
1101	48-49	0.4535612461249414
1101	50-51	0.4582130489604026
1101	52-53	0.4713602396120322
1101	54-55	0.5085271504514282
1101	56-57	0.47011469102658054
1101	58-59	0.45332647036509144
1101	60-61	0.491665080797155
1101	62-63	0.5110250126656339
1101	64-65	0.4719528264820454
1101	66-67	0.4430405193066633
1101	68-69	0.4504767212244545
1101	70-71	0.4488668155861504
1101	72-73	0.46597064211375994
1101	74-75	0.392554428217629
1101	76-77	0.4665146115197132
1101	78-79	0.4141817900798159
1101	80-81	0.448110713758588
1101	82-83	0.41329375456449213
1101	84-85	0.38593476929395365
1101	86-87	0.37936559034041295
1101	88-89	0.3798403977632461
1101	90-91	0.4838632586327094
1101	92-93	0.47949257769323594
1101	94-95	0.48183335589065734
1101	96-97	0.4999239891205889
1101	98-99	0.4888323690952703
1101	100	0.5349437772030612
1102	1	0.12776767422199953
1102	2	0.21540699830686094
1102	3	0.17134379747664497
1102	4	0.17774549893459124
1102	5	0.15187672454402446
1102	6	0.15925712270051662
1102	7	0.1741026455328054
1102	8	0.21367610162257478
1102	9	0.26904184364334327
1102	10-11	0.24345058678991194
1102	12-13	0.2519339190898151
1102	14-15	0.37207995066730604
1102	16-17	0.35971155786959486
1102	18-19	0.3458882234259235
1102	20-21	0.3353570761217668
1102	22-23	0.3489073659786115
1102	24-25	0.32292209353251167
1102	26-27	0.34721897651546385
1102	28-29	0.32353361752505805
1102	30-31	0.3269728608381399
1102	32-33	0.3260917161965651
1102	34-35	0.3392919755238779
1102	36-37	0.2958367522079328
1102	38-39	0.3209125709489271
1102	40-41	0.35876323863494974
1102	42-43	0.37012339509245606
1102	44-45	0.4107850550095762
1102	46-47	0.3468318669875501
1102	48-49	0.3238693447746783
1102	50-51	0.326922102833322
1102	52-53	0.32676202969290813
1102	54-55	0.3422049878595743
1102	56-57	0.44251672988634994
1102	58-59	0.4084265121003554
1102	60-61	0.39218866484946346
1102	62-63	0.4017206428357625
1102	64-65	0.3626329822218253
1102	66-67	0.33910183204584143
1102	68-69	0.3353481626728154
1102	70-71	0.34070505576082155
1102	72-73	0.29412269142942193
1102	74-75	0.3426101711975704
1102	76-77	0.367819182151635
1102	78-79	0.3966190274809378
1102	80-81	0.36025474207995956
1102	82-83	0.33811312333628507
1102	84-85	0.34976510081625634
1102	86-87	0.38580651735748717
1102	88-89	0.37210714258187494
1102	90-91	0.29779013323734205
1102	92-93	0.31843650387119027
1102	94-95	0.3713128572813531
1102	96-97	0.3579562495915667
1102	98-99	0.40594868524019034
1102	100	0.4166989943173931
1103	1	0.17010503077442252
1103	2	0.19774326998451386
1103	3	0.16916443011655957
1103	4	0.1560583041496173
1103	5	0.14197960716433755
1103	6	0.15320356305462468
1103	7	0.1465456588344125
1103	8	0.13988914181728518
1103	9	0.2520868658670423
1103	10-11	0.24634400835233805
1103	12-13	0.24303786683471174
1103	14-15	0.32610410284519986
1103	16-17	0.3430688823317354
1103	18-19	0.27766192460502026
1103	20-21	0.257977433399013
1103	22-23	0.23531323009679994
1103	24-25	0.2978290212910508
1103	26-27	0.29822845525748676
1103	28-29	0.2543246085840849
1103	30-31	0.2776865024239328
1103	32-33	0.2948505585097152
1103	34-35	0.27039486807865387
1103	36-37	0.2755573170045835
1103	38-39	0.28819642291058045
1103	40-41	0.34121008066186675
1103	42-43	0.34396719703192247
1103	44-45	0.30627597845489873
1103	46-47	0.3070055641057863
1103	48-49	0.24120164009540446
1103	50-51	0.2795678090149565
1103	52-53	0.3062985942273926
1103	54-55	0.3468263376725602
1103	56-57	0.34392770134637374
1103	58-59	0.3230466749233969
1103	60-61	0.35152175718307177
1103	62-63	0.3080540702892023
1103	64-65	0.3336036322628786
1103	66-67	0.34425087092239437
1103	68-69	0.2856528438737982
1103	70-71	0.3324180878615195
1103	72-73	0.30687517960613064
1103	74-75	0.3175006685059927
1103	76-77	0.3152947189380484
1103	78-79	0.31592721677349545
1103	80-81	0.2466963217716085
1103	82-83	0.25652140782155186
1103	84-85	0.29376536734477554
1103	86-87	0.3212887358800742
1103	88-89	0.3377197243823602
1103	90-91	0.3508722867941003
1103	92-93	0.36499308721464274
1103	94-95	0.3400489413678969
1103	96-97	0.3704872161168282
1103	98-99	0.38331298199179287
1103	100	0.3825136444330113
1104	1	0.11809346060949366
1104	2	0.16772481628913027
1104	3	0.12365809061512323
1104	4	0.09987474808345809
1104	5	0.10576412895657228
1104	6	0.1173577071127454
1104	7	0.13786318649716378
1104	8	0.12797399225884476
1104	9	0.2117476187955205
1104	10-11	0.21649454517574185
1104	12-13	0.19064855485489574
1104	14-15	0.2712621819984875
1104	16-17	0.28643333483073263
1104	18-19	0.2598713818401066
1104	20-21	0.267875615793713
1104	22-23	0.2731493760585906
1104	24-25	0.28489926481788075
1104	26-27	0.24685387044638674
1104	28-29	0.2518831015650065
1104	30-31	0.2625431346620388
1104	32-33	0.23816459091825948
1104	34-35	0.26698904436099014
1104	36-37	0.2672752375226679
1104	38-39	0.2832087013233817
1104	40-41	0.2864160112805152
1104	42-43	0.3226300131339528
1104	44-45	0.38739594421426204
1104	46-47	0.45827458996962633
1104	48-49	0.4231035405508834
1104	50-51	0.37712969796839246
1104	52-53	0.3953879694896614
1104	54-55	0.43078143060195373
1104	56-57	0.42441994130868466
1104	58-59	0.48054566046079117
1104	60-61	0.4197915938473713
1104	62-63	0.4686097870385382
1104	64-65	0.388740685247555
1104	66-67	0.4359600032793196
1104	68-69	0.4145944568125941
1104	70-71	0.4668038632197664
1104	72-73	0.4622562134940864
1104	74-75	0.4466165390880761
1104	76-77	0.43571984851893575
1104	78-79	0.425154915864546
1104	80-81	0.4071616941077707
1104	82-83	0.44155120098662337
1104	84-85	0.4279412513991545
1104	86-87	0.4272435303871873
1104	88-89	0.35358639580788065
1104	90-91	0.4154281128302628
1104	92-93	0.4085285312587388
1104	94-95	0.43252932505699704
1104	96-97	0.5036970085238153
1104	98-99	0.5124384921332492
1104	100	0.5497965951309922
1105	1	0.1041669468265809
1105	2	0.11773098751131528
1105	3	0.10463510759036154
1105	4	0.08940856237985173
1105	5	0.0801805200992689
1105	6	0.11672440132317519
1105	7	0.12838400093279034
1105	8	0.11106813856040532
1105	9	0.1578934198742914
1105	10-11	0.15507797242682386
1105	12-13	0.11395831076163887
1105	14-15	0.1690463696043949
1105	16-17	0.1872492541926789
1105	18-19	0.16310711226568486
1105	20-21	0.15072871726357562
1105	22-23	0.17901858022888462
1105	24-25	0.21729460846833604
1105	26-27	0.17686194434439528
1105	28-29	0.2087461831862143
1105	30-31	0.21649534351239197
1105	32-33	0.18098608499798985
1105	34-35	0.25192516162005063
1105	36-37	0.24987458925718897
1105	38-39	0.2632130094107481
1105	40-41	0.21501086588397555
1105	42-43	0.23917552582695123
1105	44-45	0.3084481154724017
1105	46-47	0.2719825266885678
1105	48-49	0.2707237318107545
1105	50-51	0.2147010353668506
1105	52-53	0.2257340672578252
1105	54-55	0.2764147848763301
1105	56-57	0.26509010779422937
1105	58-59	0.21394535051574337
1105	60-61	0.21766041804738023
1105	62-63	0.24585077623039098
1105	64-65	0.22831976458782322
1105	66-67	0.2615921298994266
1105	68-69	0.2860166425022612
1105	70-71	0.30408262066794833
1105	72-73	0.2545335950092138
1105	74-75	0.2978183410661259
1105	76-77	0.318209681065575
1105	78-79	0.29251157004724604
1105	80-81	0.2573384847954472
1105	82-83	0.22988600471210674
1105	84-85	0.26074629641800584
1105	86-87	0.22763981134791322
1105	88-89	0.25350710666489107
1105	90-91	0.21172119947036094
1105	92-93	0.22345454767220474
1105	94-95	0.28748662405944003
1105	96-97	0.22993282283282213
1105	98-99	0.2494521741705995
1105	100	0.2816738715046867
1106	1	0.09299239221343925
1106	2	0.1454893854428292
1106	3	0.14190224038613053
1106	4	0.13724058024133967
1106	5	0.09626349862411843
1106	6	0.1380984532641918
1106	7	0.132869744090506
1106	8	0.18601783551107332
1106	9	0.17672869726431628
1106	10-11	0.1986288214057339
1106	12-13	0.18684238611719195
1106	14-15	0.2560990817142539
1106	16-17	0.23381216290010798
1106	18-19	0.23885238698701272
1106	20-21	0.1977194360138057
1106	22-23	0.19141068666466055
1106	24-25	0.21246100834207482
1106	26-27	0.24508467249027888
1106	28-29	0.23120282054338048
1106	30-31	0.29204950777179306
1106	32-33	0.2404845767864643
1106	34-35	0.2784592781020052
1106	36-37	0.25728479756869405
1106	38-39	0.24768904585673113
1106	40-41	0.2981300472301456
1106	42-43	0.3154000220227289
1106	44-45	0.3260018639904061
1106	46-47	0.32389360121612754
1106	48-49	0.3230168995998852
1106	50-51	0.32242858844551137
1106	52-53	0.32510329148485795
1106	54-55	0.31186645491166587
1106	56-57	0.34801847503737804
1106	58-59	0.33254048964968774
1106	60-61	0.3774903156934002
1106	62-63	0.35999960617896676
1106	64-65	0.36658989356303096
1106	66-67	0.3567956101998533
1106	68-69	0.37001190637685966
1106	70-71	0.35563484559111913
1106	72-73	0.4027786268530207
1106	74-75	0.400039554735919
1106	76-77	0.41626171760478314
1106	78-79	0.3480203231854304
1106	80-81	0.33180751495207517
1106	82-83	0.2882976116005693
1106	84-85	0.28243412861240813
1106	86-87	0.2733012099325407
1106	88-89	0.29869867607278877
1106	90-91	0.31374041034847266
1106	92-93	0.3163427581920075
1106	94-95	0.351716618050375
1106	96-97	0.317398982976826
1106	98-99	0.40264404260008746
1106	100	0.43006788981105615
1107	1	0.08220381305186919
1107	2	0.1341503325078648
1107	3	0.08373031305664114
1107	4	0.11594349689243444
1107	5	0.09352676118627556
1107	6	0.10246216859745516
1107	7	0.06364989474276683
1107	8	0.0239302147101057
1107	9	0.09752897266944416
1107	10-11	0.09571407795304765
1107	12-13	0.04343330833207659
1107	14-15	0.1883396315695336
1107	16-17	0.13459534289840747
1107	18-19	0.11504525485906214
1107	20-21	0.11971299655934331
1107	22-23	0.11569607643163948
1107	24-25	0.12426135934956051
1107	26-27	0.1594386658045721
1107	28-29	0.13732120492266375
1107	30-31	0.16186642608236212
1107	32-33	0.1837783095794876
1107	34-35	0.19733073663194034
1107	36-37	0.18016771165733303
1107	38-39	0.04652389372807164
1107	40-41	0.10715048476293276
1107	42-43	0.10884113667109574
1107	44-45	0.12148246621026715
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2216	74-75	-0.7995448636421756
2216	76-77	-0.7485317839333163
2216	78-79	-0.6745481224232535
2216	80-81	-0.7865992092669529
2216	82-83	-0.7961750706749484
2216	84-85	-0.7729865337435626
2216	86-87	-0.8030660906202556
2216	88-89	-0.8255259834474913
2216	90-91	-0.7937657065117278
2216	92-93	-0.7441644117508197
2216	94-95	-0.8288778814887685
2216	96-97	-0.9844277387828981
2216	98-99	-0.9933821791522419
2216	100	-1.0257122340848497
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94.0
3	259.0
4	201.0
5	250.0
6	584.0
7	1403.0
8	2555.0
9	3950.0
10	5503.0
11	7273.0
12	9730.0
13	13018.0
14	13747.0
15	16961.0
16	21606.0
17	29895.0
18	31096.0
19	20782.0
20	17305.0
21	18128.0
22	19615.0
23	21813.0
24	25507.0
25	31973.0
26	41681.0
27	54551.0
28	70067.0
29	89681.0
30	114052.0
31	143877.0
32	180098.0
33	230416.0
34	311264.0
35	461654.0
36	777648.0
37	1490679.0
38	2023191.0
39	796631.0
40	4494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.29500117607787	19.794024397629816	13.56469907195484	27.346275354337475
2	14.07864757901755	20.59784897916892	38.02381789022237	27.299685551591164
3	15.810278182249995	25.575566352222463	30.526601945793324	28.087553519734215
4	11.67950389483968	17.51778369922075	36.54790205529482	34.254810350644746
5	13.241764971722777	36.47575845888082	34.08028281446003	16.20219375493637
6	31.186789337586045	36.83611910747108	17.68352772371788	14.293563831224997
7	27.32056337171586	31.616776701084802	22.242579152701193	18.820080774498145
8	26.175929492377552	34.41702312412153	20.26456407449454	19.142483309006376
9	25.539824688254587	16.09802411071467	20.951350033336936	37.410801167693805
10-11	24.581023717062557	25.807662006708803	28.389626383701817	21.221687892526823
12-13	25.18154411963455	23.598518251973186	28.403218422261865	22.816719206130394
14-15	22.692191385555194	25.18585201778571	25.91067699886474	26.211279597794356
16-17	22.2182662765344	27.970992641096338	26.435100247324034	23.375640835045232
18-19	22.29794831423217	26.76609746098677	28.71739230817746	22.218561916603598
20-21	23.32067289932245	26.067542211770643	28.419950242368547	22.191834646538364
22-23	22.90038534571305	26.09972474501748	28.309240075503656	22.690649833765814
24-25	22.74570331860262	26.0968054403197	28.483551499534965	22.67393974154272
26-27	22.840377450715394	25.96810156278156	28.467435668720942	22.7240853177821
28-29	22.761866234730586	26.039137367654053	28.52099363787774	22.67800275973762
30-31	22.90913473210886	26.32090002598216	28.29979597914979	22.47016926275919
32-33	22.618821363701507	26.218453418207442	28.483086417354592	22.679638800736456
34-35	22.746816674283053	25.839449671735153	28.4327799697766	22.98095368420519
36-37	22.958415127613154	26.17486174546383	28.283843086477745	22.58288004044527
38-39	22.935576456191935	26.233930858397276	28.177615426882756	22.652877258528033
40-41	22.845298418885246	26.02801178575205	28.308998834742827	22.81769096061988
42-43	22.681101122587872	26.23641675148296	28.27392250689541	22.808559619033755
44-45	22.85725869979037	26.38112667452835	27.98280613548918	22.778808490192105
46-47	22.875415903982457	26.363054295795052	27.820643422501572	22.940886377720922
48-49	22.865392856519396	26.29206482032615	27.8247956837506	23.017746639403853
50-51	22.88360947585679	26.217346152760502	27.710639492431888	23.188404878950823
52-53	22.856760533330256	26.369138829891437	27.615123286004987	23.15897735077332
54-55	22.970073046341895	26.70726783367745	27.43711884932372	22.885540270656925
56-57	22.58606486924684	26.454878216525824	27.624809388846188	23.334247525381148
58-59	22.693685140801314	26.143514829857306	27.797670345086452	23.365129684254928
60-61	23.040769540707736	26.47013731661999	27.555726716021322	22.933366426650952
62-63	22.831115413487048	26.455254727122934	27.689850785268078	23.02377907412194
64-65	22.861100855499824	26.176028055129002	27.78728333139171	23.175587757979464
66-67	22.598871090482614	26.428412496771713	27.692915325169487	23.27980108757619
68-69	22.93583838957774	26.28540130720316	27.57528828107353	23.203472022145572
70-71	22.939185695810153	26.120833755971162	27.647883761774878	23.29209678644381
72-73	22.75447289346596	26.3936472861931	27.696371173009695	23.155508647331242
74-75	22.795453102446398	26.239427853604923	27.590801463618526	23.37431758033015
76-77	22.83103663233863	26.26784539770065	27.351281782715247	23.549836187245468
78-79	22.742692162795453	26.548859133269026	27.4778423684212	23.230606335514324
80-81	22.657707083198183	26.281733441903633	27.58723775318052	23.473321721717664
82-83	22.622792266253516	26.28286303762201	27.66092672903801	23.433417967086456
84-85	22.715092866328362	26.354814915291268	27.367859259359896	23.562232959020474
86-87	22.837561831008756	26.420085955238402	27.291076794338125	23.45127541941471
88-89	22.561961935074063	26.317829686542687	27.466447667765888	23.653760710617362
90-91	22.480395014578928	26.30018464131431	27.420266395653865	23.799153948452897
92-93	22.664130068980732	26.36721355279169	27.325265578666453	23.64339079956113
94-95	22.568986906242117	26.17061501018128	27.446484924045844	23.81391315953076
96-97	22.413775020305973	26.33329417260982	27.374162270822662	23.878768536261553
98-99	22.430967865096775	26.29360264686506	27.45963418858456	23.815795299453605
100	22.4361411863286	26.14846332129605	27.4556049244785	23.959790567896846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2312.0
1	1442.5
2	4885.0
3	8179.5
4	4735.0
5	2179.5
6	2950.0
7	7159.5
8	10959.0
9	10963.5
10	7911.0
11	4754.5
12	4241.0
13	4491.0
14	4871.5
15	5219.5
16	5682.0
17	6378.5
18	7085.5
19	8010.0
20	9610.5
21	11524.0
22	13946.0
23	17654.0
24	23189.5
25	29490.5
26	37385.5
27	47388.5
28	59965.5
29	74944.0
30	87502.0
31	99522.5
32	112771.5
33	123998.5
34	134439.0
35	145149.5
36	156472.5
37	168136.5
38	179329.5
39	188040.0
40	196788.0
41	207126.5
42	213733.0
43	218542.5
44	222957.5
45	227633.0
46	232424.0
47	234042.0
48	233487.5
49	233855.5
50	237312.5
51	240507.0
52	240375.0
53	241125.0
54	239754.0
55	230605.0
56	216706.5
57	197902.0
58	182957.0
59	174674.0
60	159404.0
61	138425.5
62	118525.5
63	97647.0
64	76556.0
65	59908.5
66	46794.5
67	36342.0
68	28593.5
69	21936.5
70	16190.0
71	11883.0
72	9278.5
73	7487.5
74	5959.5
75	4311.0
76	2767.5
77	1865.5
78	1348.0
79	914.0
80	492.0
81	183.5
82	79.0
83	40.0
84	20.5
85	15.5
86	11.0
87	9.0
88	9.0
89	7.5
90	3.5
91	0.5
92	0.5
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04733056726853354
2	0.0
3	1.5485908386492233E-4
4	0.06668795275164882
5	0.004814709698345767
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.010861253018344326
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.546315817926263E-4
26-27	0.0
28-29	2.886010199300825E-4
30-31	0.003962984737088694
32-33	8.446859119904854E-5
34-35	0.0017738404151800193
36-37	0.004230468609219015
38-39	0.001393731754784301
40-41	0.001112169784120806
42-43	0.0019075823512451798
44-45	7.039049266587378E-6
46-47	4.223429559952427E-4
48-49	3.941867589288932E-4
50-51	0.0014993174937831116
52-53	7.531782715248496E-4
54-55	9.85466897322233E-4
56-57	0.005926879482466573
58-59	0.008939592568565972
60-61	0.0012107164738530292
62-63	0.0025340577359714562
64-65	0.0010488183407215195
66-67	7.742954193246117E-5
68-69	7.250220744585E-4
70-71	3.730696111291311E-4
72-73	0.0
74-75	2.1117147799762134E-4
76-77	0.0
78-79	5.068115471942912E-4
80-81	0.0
82-83	0.0010840135870544563
84-85	5.420067935272281E-4
86-87	0.0
88-89	0.0
90-91	7.039049266587378E-6
92-93	2.322886257973835E-4
94-95	0.0
96-97	3.730696111291311E-4
98-99	5.138505964608787E-4
100	3.2379626626301945E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7103232.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.79002145768419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.14229127684432	36.133185004997415
2	16.26499147990464	16.521985325468144
3	5.327872109812529	8.118082163435265
4	2.372878356751053	4.820741706234415
5	1.305693712167502	3.3158105829075377
6	0.7833646306451889	2.3872263839795993
7	0.5249810597256499	1.8664659501840475
8	0.37201968317107953	1.5115910152752001
9	0.2786062657204617	1.2735376392768762
>10	1.4607131479960564	13.440436071053316
>50	0.10811104277506749	3.76504168713834
>100	0.056088124520114274	4.932914471636604
>500	0.0019724345184739694	0.6700675604672253
>1k	2.7778363397024537E-4	0.2778413802964168
>5k	2.7778362804407486E-5	0.09045046814601078
>10k+	1.1111345121762994E-4	0.8746225895035877
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17760	0.2500270299491837	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15704	0.2210824593649764	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14886	0.20956657476483947	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13642	0.19205342018957006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	1.4078098533174758E-5	0.0	0.0	7.039049266587379E-6
16-17	0.0	2.8156197066349515E-5	0.0	1.4078098533174758E-5	1.4078098533174758E-5
18-19	0.0	4.927334486611165E-5	0.0	1.4078098533174758E-5	1.4078098533174758E-5
20-21	0.0	7.039049266587379E-5	0.0	3.5195246332936895E-5	1.4078098533174758E-5
22-23	0.0	7.039049266587379E-5	0.0	1.5485908386492233E-4	1.4078098533174758E-5
24-25	0.0	7.039049266587379E-5	0.0	4.927334486611165E-4	1.4078098533174758E-5
26-27	0.0	7.039049266587379E-5	0.0	8.306078134573108E-4	1.4078098533174758E-5
28-29	0.0	7.742954193246117E-5	0.0	0.0018653480556456554	1.4078098533174758E-5
30-31	0.0	8.446859119904854E-5	0.0	0.004856943993945291	1.4078098533174758E-5
32-33	0.0	8.446859119904854E-5	0.0	0.012184594280462752	1.4078098533174758E-5
34-35	0.0	8.446859119904854E-5	0.0	0.023207745431938587	1.4078098533174758E-5
36-37	0.0	8.446859119904854E-5	0.0	0.04072793905647458	1.4078098533174758E-5
38-39	0.0	8.446859119904854E-5	0.0	0.06728627193930875	1.4078098533174758E-5
40-41	0.0	8.446859119904854E-5	0.0	0.09976444525534292	1.4078098533174758E-5
42-43	0.0	9.854668973222331E-5	0.0	0.13333367120769812	1.4078098533174758E-5
44-45	1.4078098533174758E-5	9.854668973222331E-5	0.0	0.17363926730817747	1.4078098533174758E-5
46-47	1.4078098533174758E-5	9.854668973222331E-5	0.0	0.22129363084297402	1.4078098533174758E-5
48-49	1.4078098533174758E-5	9.854668973222331E-5	0.0	0.2741146565394457	1.4078098533174758E-5
50-51	1.4078098533174758E-5	1.1262478826539806E-4	0.0	0.32931488088802396	1.4078098533174758E-5
52-53	1.4078098533174758E-5	1.2670288679857283E-4	0.0	0.38674648385411037	1.4078098533174758E-5
54-55	1.4078098533174758E-5	1.3374193606516022E-4	0.0	0.4524419306591704	1.4078098533174758E-5
56-57	1.4078098533174758E-5	1.4078098533174758E-4	0.0	0.5294139343893034	1.4078098533174758E-5
58-59	1.4078098533174758E-5	1.4078098533174758E-4	0.0	0.6158464203337297	1.4078098533174758E-5
60-61	1.4078098533174758E-5	1.4078098533174758E-4	0.0	0.7077764037553609	1.4078098533174758E-5
62-63	1.4078098533174758E-5	1.5485908386492233E-4	0.0	0.8046337216636033	1.4078098533174758E-5
64-65	1.4078098533174758E-5	1.6893718239809709E-4	0.0	0.9106629207662089	1.4078098533174758E-5
66-67	1.4078098533174758E-5	1.6893718239809709E-4	0.0	1.033606955256424	1.4078098533174758E-5
68-69	1.4078098533174758E-5	1.6893718239809709E-4	0.0	1.1677149218834468	1.4078098533174758E-5
70-71	1.4078098533174758E-5	1.6893718239809709E-4	0.0	1.317252484502829	1.4078098533174758E-5
72-73	1.4078098533174758E-5	1.6893718239809709E-4	0.0	1.4820788621292391	1.4078098533174758E-5
74-75	1.4078098533174758E-5	1.6893718239809709E-4	0.0	1.6489817592892926	1.4078098533174758E-5
76-77	1.4078098533174758E-5	1.6893718239809709E-4	0.0	1.8306877770569792	1.4078098533174758E-5
78-79	1.4078098533174758E-5	1.6893718239809709E-4	0.0	2.034820205788013	1.4078098533174758E-5
80-81	1.4078098533174758E-5	1.6893718239809709E-4	0.0	2.2626460743503802	1.4078098533174758E-5
82-83	1.4078098533174758E-5	1.6893718239809709E-4	0.0	2.5054017664071795	1.4078098533174758E-5
84-85	1.4078098533174758E-5	1.6893718239809709E-4	0.0	2.749727166450427	1.4078098533174758E-5
86-87	1.4078098533174758E-5	1.6893718239809709E-4	0.0	3.0107914256496198	1.4078098533174758E-5
88	1.4078098533174758E-5	1.6893718239809709E-4	0.0	3.228220618445237	1.4078098533174758E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9590	0.0	47.83565	1
TATAACG	955	0.0	47.77082	2
TAACGCA	1140	0.0	39.605972	4
GTATCAA	16190	0.0	36.937237	1
ATAACGC	1400	0.0	33.594357	3
TACCTGG	6545	0.0	30.61218	2
TATCACG	1085	0.0	29.04286	2
ATCAACG	20555	0.0	28.555607	3
TCAACGC	20680	0.0	28.42849	4
GTACCTG	7400	0.0	27.276665	1
CAACGCA	21710	0.0	27.174278	5
TATCAAC	21770	0.0	26.961899	2
AACGCAG	23085	0.0	25.881155	6
GTATAAC	2310	0.0	25.663994	1
ACCTGGG	8355	0.0	23.473827	3
ACGCAGA	26165	0.0	22.798635	7
GTACATG	19630	0.0	22.386814	1
CGCAGAG	26835	0.0	22.36955	8
AGAGTAC	27880	0.0	20.763933	10-11
TACATGG	21160	0.0	20.270922	2
>>END_MODULE