FastQCFastQC Report
Wed 25 May 2016
SRR2431427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527338
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA279440.7922121441154775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.052.4108281
GTATCAA90350.038.949241
ATCAACG102600.033.332623
TCAACGC103050.033.3221174
CAACGCA110050.031.1166555
TATCAAC114050.030.3574262
AACGCAG122450.028.055356
TACCTGG26950.026.7063832
GTACATG190750.025.946381
CGCAGAG130050.025.7284978
TACATGG190250.025.047662
ACGCAGA136150.024.8522537
CATGGGT88800.023.8373154
ACATGGG193950.023.745173
GTACCTG30950.023.7131651
TCTGTCG6350.023.6821448
GTGGTAT24250.021.7286051
GCAGAGT146500.021.6847219
ATGGGTG47000.021.5175195
CTGTCGC7250.020.0940959
Produced by FastQC (version 0.11.5)