##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431427_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3527338
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.579598269289757	31.0	31.0	34.0	26.0	34.0
2	30.68285659043732	33.0	31.0	34.0	26.0	34.0
3	30.80496311949691	34.0	31.0	34.0	26.0	34.0
4	33.99025043814911	37.0	35.0	37.0	28.0	37.0
5	34.148764308949126	37.0	35.0	37.0	30.0	37.0
6	34.154521625089515	37.0	35.0	37.0	30.0	37.0
7	34.07865308059505	37.0	35.0	37.0	30.0	37.0
8	33.999306275724074	37.0	35.0	37.0	30.0	37.0
9	35.415790888199545	39.0	37.0	39.0	28.0	39.0
10-11	35.4438399438897	39.0	37.0	39.0	28.5	39.0
12-13	35.375398671746225	39.0	37.0	39.0	27.5	39.0
14-15	36.722473292891124	41.0	38.0	41.0	27.5	41.0
16-17	36.760039298757306	40.5	38.0	41.0	29.0	41.0
18-19	36.79738474169473	40.5	38.0	41.0	29.0	41.0
20-21	36.88187834565329	41.0	38.0	41.0	29.0	41.0
22-23	36.87280904183268	40.0	38.0	41.0	29.0	41.0
24-25	36.93515818444391	40.5	38.0	41.0	29.0	41.0
26-27	36.976097413970535	40.0	38.0	41.0	30.0	41.0
28-29	36.93988214341807	40.0	38.0	41.0	30.0	41.0
30-31	36.87366237655705	40.0	38.0	41.0	30.0	41.0
32-33	36.81012437707983	40.0	38.0	41.0	30.0	41.0
34-35	36.731534375214395	40.0	37.5	41.0	30.0	41.0
36-37	36.602180879745575	40.0	37.0	41.0	29.0	41.0
38-39	36.48169880516129	40.0	37.0	41.0	29.0	41.0
40-41	36.41831389563461	40.0	37.0	41.0	28.5	41.0
42-43	36.27029972744319	40.0	36.5	41.0	28.0	41.0
44-45	36.14493507568597	40.0	36.0	41.0	27.5	41.0
46-47	36.02710103199637	40.0	36.0	41.0	27.0	41.0
48-49	35.9001810997415	40.0	36.0	41.0	27.0	41.0
50-51	35.77774174178941	40.0	35.0	41.0	27.0	41.0
52-53	35.62770352600177	40.0	35.0	41.0	27.0	41.0
54-55	35.48110728260235	40.0	35.0	41.0	27.0	41.0
56-57	35.30681465739887	39.0	35.0	41.0	27.0	41.0
58-59	35.10586680380502	39.0	35.0	41.0	26.0	41.0
60-61	34.883815784027504	39.0	34.0	41.0	26.0	41.0
62-63	34.791944378451966	39.0	34.0	41.0	26.0	41.0
64-65	34.5578448960661	38.0	34.0	40.5	26.0	41.0
66-67	34.30619067410041	38.0	34.0	40.0	26.0	41.0
68-69	34.01555861672456	37.0	34.0	40.0	25.0	41.0
70-71	33.68941337631948	37.0	33.5	40.0	24.5	41.0
72-73	33.34910944173765	36.0	33.0	39.0	23.5	41.0
74-75	33.05853422609344	36.0	33.0	39.0	23.0	41.0
76-77	32.77662659489961	35.5	33.0	39.0	22.5	40.5
78-79	32.420621868389134	35.0	33.0	37.5	22.0	39.5
80-81	32.083937660638135	35.0	33.0	37.0	20.5	39.0
82-83	31.745064975344015	35.0	32.0	37.0	20.0	39.0
84-85	31.433757411396357	35.0	32.0	36.0	19.0	38.0
86-87	31.140428702891526	35.0	32.0	36.0	17.5	37.0
88-89	30.869402081683127	35.0	32.0	35.5	13.5	37.0
90-91	30.634543244792532	35.0	32.0	35.0	7.5	36.5
92-93	30.40478216150536	35.0	31.5	35.0	2.0	36.0
94-95	30.193429436022292	35.0	31.0	35.0	2.0	36.0
96-97	29.998754868402177	35.0	31.0	35.0	2.0	36.0
98-99	29.79180560524679	35.0	31.0	35.0	2.0	35.5
100	29.62964535862455	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33710171264224087
1101	2	0.3627220913795739
1101	3	0.3593267172684307
1101	4	0.4640764611425112
1101	5	0.3985714172074708
1101	6	0.45504520548910676
1101	7	0.3994454180324851
1101	8	0.4673044660618899
1101	9	0.4906161631541295
1101	10-11	0.4186829722259944
1101	12-13	0.3246972644814079
1101	14-15	0.4338833297488449
1101	16-17	0.444490503066703
1101	18-19	0.33858518916925107
1101	20-21	0.38060705357383284
1101	22-23	0.43602898250215816
1101	24-25	0.3307723077593252
1101	26-27	0.37238056954465293
1101	28-29	0.32051178828587723
1101	30-31	0.36457520223471107
1101	32-33	0.3078091972670407
1101	34-35	0.332259780395205
1101	36-37	0.33065299325881625
1101	38-39	0.39169222374719226
1101	40-41	0.3467656945843842
1101	42-43	0.35994876675948007
1101	44-45	0.27629850859020166
1101	46-47	0.23876716595689373
1101	48-49	0.2924448581810708
1101	50-51	0.2762382247562627
1101	52-53	0.1926472236434691
1101	54-55	0.21461842971596923
1101	56-57	0.14952177251985432
1101	58-59	0.19164331925394862
1101	60-61	0.15470158224820807
1101	62-63	0.09384033747274856
1101	64-65	0.13074733122439852
1101	66-67	0.17505034887458493
1101	68-69	0.2580407491964536
1101	70-71	0.16147907479963663
1101	72-73	0.14934419192025672
1101	74-75	0.1938499917963199
1101	76-77	0.207093839536725
1101	78-79	0.1834764633746886
1101	80-81	0.14759338558291546
1101	82-83	0.19951193306032877
1101	84-85	0.16759769296197646
1101	86-87	0.1310575306750934
1101	88-89	0.09110079748904099
1101	90-91	0.10651432424434404
1101	92-93	0.06851996926801007
1101	94-95	0.14077279983598956
1101	96-97	0.18199667969892275
1101	98-99	0.14762390356477795
1101	100	0.12600077998451553
1102	1	0.08542932646349399
1102	2	0.14108420312327397
1102	3	0.14826851182034062
1102	4	0.26377391026974095
1102	5	0.1816410612566841
1102	6	0.201184098573151
1102	7	0.12180230384699087
1102	8	-0.003415687984201554
1102	9	0.1620437974717106
1102	10-11	0.14678737674736908
1102	12-13	0.11700465293188245
1102	14-15	0.1293458043346689
1102	16-17	0.06765630376877851
1102	18-19	0.1304084073847065
1102	20-21	0.13339271797850216
1102	22-23	0.15833167281977012
1102	24-25	0.15977901078847623
1102	26-27	0.1620216704259363
1102	28-29	0.15376812818733754
1102	30-31	0.14529665237215994
1102	32-33	0.2325807512504383
1102	34-35	0.2219420405056738
1102	36-37	0.12734498182782517
1102	38-39	0.15402000382349001
1102	40-41	0.16128484865712522
1102	42-43	0.1876028826506655
1102	44-45	0.0879183919641946
1102	46-47	0.06496657000869988
1102	48-49	0.055186530833765346
1102	50-51	-0.010968679333608122
1102	52-53	0.05910942013282039
1102	54-55	0.014206536007520754
1102	56-57	-0.06333525543637819
1102	58-59	-0.1310568760957338
1102	60-61	-0.07245710787776005
1102	62-63	0.03058398012085206
1102	64-65	0.02768637431871923
1102	66-67	0.019705431519845718
1102	68-69	-0.02471998250344143
1102	70-71	-0.07395122039191193
1102	72-73	-0.02544346405489506
1102	74-75	0.02252817056745471
1102	76-77	0.01249510196460335
1102	78-79	0.052826092029448546
1102	80-81	0.06361880166472389
1102	82-83	0.061771331229746096
1102	84-85	-0.0886022480872839
1102	86-87	-0.015674526470959904
1102	88-89	-0.07972247672644883
1102	90-91	-0.11554279086887576
1102	92-93	-0.06003444626300691
1102	94-95	0.00640157944144093
1102	96-97	-0.04762268032067496
1102	98-99	-0.0938804515877365
1102	100	-0.19544716822513664
1103	1	-0.2761735136059045
1103	2	-0.23276584926923505
1103	3	-0.17029794449369717
1103	4	-0.09588513991833025
1103	5	-0.12055319941582354
1103	6	-0.11938630942115225
1103	7	-0.06387021393432946
1103	8	-0.11187018929154391
1103	9	-0.1013043217113605
1103	10-11	-0.0786489623384341
1103	12-13	-0.07233449094486133
1103	14-15	-0.15280052151708645
1103	16-17	-0.1321296777752181
1103	18-19	-0.1720923772672549
1103	20-21	-0.19202681596294724
1103	22-23	-0.21082000565580472
1103	24-25	-0.15425302988739276
1103	26-27	-0.15067731144181096
1103	28-29	-0.17398779191902491
1103	30-31	-0.1434528749831614
1103	32-33	-0.13378101068245485
1103	34-35	-0.14964684727797817
1103	36-37	-0.22011385020228147
1103	38-39	-0.1295306636867224
1103	40-41	-0.10721459559922408
1103	42-43	-0.17008905433992538
1103	44-45	-0.25720779953290673
1103	46-47	-0.3369217767975883
1103	48-49	-0.23782537116259306
1103	50-51	-0.31114539592967816
1103	52-53	-0.3101626591701603
1103	54-55	-0.23744864714383596
1103	56-57	-0.21128099644438691
1103	58-59	-0.27394203672078987
1103	60-61	-0.26932728875548406
1103	62-63	-0.1551714821688961
1103	64-65	-0.2421575201004913
1103	66-67	-0.18542429245565728
1103	68-69	-0.1972308131718421
1103	70-71	-0.286996391322468
1103	72-73	-0.34000004765554337
1103	74-75	-0.3115456651820381
1103	76-77	-0.31073296701310227
1103	78-79	-0.29491234949112055
1103	80-81	-0.2530177660822517
1103	82-83	-0.2828169952497248
1103	84-85	-0.35795372706355266
1103	86-87	-0.2954188596774294
1103	88-89	-0.39590929834231403
1103	90-91	-0.43940808064640535
1103	92-93	-0.42650320970094313
1103	94-95	-0.4199893011110518
1103	96-97	-0.34413099454227236
1103	98-99	-0.3915640060858294
1103	100	-0.37266513836140547
1104	1	0.014453671296010384
1104	2	0.07585205238358128
1104	3	0.044012335293178495
1104	4	0.13911595892734852
1104	5	0.2684365342694761
1104	6	0.16245653807481375
1104	7	0.2063174524757514
1104	8	0.07014338436226808
1104	9	0.1871401197916711
1104	10-11	0.22507835338279136
1104	12-13	0.16421337867521402
1104	14-15	0.09533144083416545
1104	16-17	0.06222976365973665
1104	18-19	0.13400074672780704
1104	20-21	0.023999511603520318
1104	22-23	0.1595796668433067
1104	24-25	0.09793068341681277
1104	26-27	0.058735440934206906
1104	28-29	0.07646243432755284
1104	30-31	0.11758947808170461
1104	32-33	0.08459923906322331
1104	34-35	0.09281196768942124
1104	36-37	0.009129871638613452
1104	38-39	0.0442587974818025
1104	40-41	0.05995427282859822
1104	42-43	0.04223023803540826
1104	44-45	0.01888291985766699
1104	46-47	-0.043248404547846064
1104	48-49	-0.11877439955708269
1104	50-51	-0.10699881105760056
1104	52-53	-0.19861449477493665
1104	54-55	-0.14698396132051528
1104	56-57	-0.15538495340706504
1104	58-59	-0.22586837518823444
1104	60-61	-0.22177146157572736
1104	62-63	-0.23079910790229263
1104	64-65	-0.18959991019671918
1104	66-67	-0.14247136968210583
1104	68-69	-0.1814900105177486
1104	70-71	-0.11828921501962242
1104	72-73	-0.2268562749003351
1104	74-75	-0.10723296025149409
1104	76-77	-0.03276820036433037
1104	78-79	-0.07504955191751606
1104	80-81	-0.0823058525336009
1104	82-83	-0.16312541068839437
1104	84-85	-0.06745892472911663
1104	86-87	-0.005510013281295301
1104	88-89	-0.05259611469694292
1104	90-91	-0.0750325661686837
1104	92-93	-0.1391100694951568
1104	94-95	-0.1627593605978177
1104	96-97	-0.10282136523856522
1104	98-99	-0.15059342525403707
1104	100	-0.0837507301858409
1105	1	-0.10349546168512092
1105	2	-0.011546131266346293
1105	3	0.060999853594953635
1105	4	0.11077524056369015
1105	5	0.030393607222350738
1105	6	0.0684680325925413
1105	7	0.11260851760116708
1105	8	0.09130153313038392
1105	9	0.10409837947238998
1105	10-11	0.02685912242860411
1105	12-13	0.16885807598810487
1105	14-15	0.12869909525264944
1105	16-17	0.10513562259927767
1105	18-19	0.11034114287961927
1105	20-21	0.14255368093431997
1105	22-23	0.10802497768295183
1105	24-25	0.08259601424372676
1105	26-27	0.17029803707325897
1105	28-29	0.13554534436056542
1105	30-31	0.19703902553789732
1105	32-33	0.1948183147382423
1105	34-35	0.12121085898707662
1105	36-37	0.07634471463203596
1105	38-39	0.04755853656058662
1105	40-41	-0.010671566162265833
1105	42-43	-0.09150286726758594
1105	44-45	-0.08525580724740678
1105	46-47	-0.0874737580567313
1105	48-49	-0.16862582304594298
1105	50-51	-0.04615344084561457
1105	52-53	-0.07504954538462982
1105	54-55	-0.14354530716714464
1105	56-57	-0.10362216594621998
1105	58-59	-0.04004707343650438
1105	60-61	-0.1554073811887946
1105	62-63	-0.0011929210717127603
1105	64-65	0.026994684461271845
1105	66-67	0.013014742839978055
1105	68-69	0.05153829611032279
1105	70-71	0.15753469628479166
1105	72-73	0.12803802875678372
1105	74-75	0.04613900596378073
1105	76-77	0.013256068362153428
1105	78-79	-0.01020561069764625
1105	80-81	-0.04087357549588688
1105	82-83	0.06432325079874346
1105	84-85	-0.03938409923940256
1105	86-87	0.02327068164368029
1105	88-89	0.09448808569687728
1105	90-91	0.019845559548191005
1105	92-93	0.015031822595886979
1105	94-95	0.03734141951730763
1105	96-97	0.06885021689913984
1105	98-99	-0.018440296838502235
1105	100	-0.019075972884266434
1106	1	-0.26125298118616413
1106	2	-0.2621021851234495
1106	3	-0.2488448544703452
1106	4	-0.17762502308593042
1106	5	-0.08431291465869606
1106	6	-0.14671938243950677
1106	7	-0.14432840151593496
1106	8	-0.24270999660293313
1106	9	-0.26699560038861136
1106	10-11	-0.2267217416182703
1106	12-13	-0.17178173555590348
1106	14-15	-0.2722701325935617
1106	16-17	-0.284265813537381
1106	18-19	-0.3122612679513921
1106	20-21	-0.28982905307053386
1106	22-23	-0.1973725940287423
1106	24-25	-0.18427828827331183
1106	26-27	-0.22495545975903752
1106	28-29	-0.18855003182557795
1106	30-31	-0.16099894623594224
1106	32-33	-0.2332077686879046
1106	34-35	-0.17959263176290818
1106	36-37	-0.26816815807439554
1106	38-39	-0.2847754094672865
1106	40-41	-0.2896813740213773
1106	42-43	-0.27676647195086446
1106	44-45	-0.231638600947349
1106	46-47	-0.23300426933113272
1106	48-49	-0.29332243310077644
1106	50-51	-0.2805782277861013
1106	52-53	-0.31571315359635577
1106	54-55	-0.24716411310331665
1106	56-57	-0.31187731282629017
1106	58-59	-0.39274518245208867
1106	60-61	-0.41460829396889665
1106	62-63	-0.3766333989076145
1106	64-65	-0.27779767001038635
1106	66-67	-0.2247228072845786
1106	68-69	-0.24244501197341606
1106	70-71	-0.3248136427695627
1106	72-73	-0.3283322936991553
1106	74-75	-0.28686486397207744
1106	76-77	-0.23447362264843008
1106	78-79	-0.23024604432071527
1106	80-81	-0.21917852265445248
1106	82-83	-0.20656103433534412
1106	84-85	-0.20082925156341602
1106	86-87	-0.2752725325168228
1106	88-89	-0.32674969682798505
1106	90-91	-0.288548546704412
1106	92-93	-0.24935289133314242
1106	94-95	-0.29245332146350833
1106	96-97	-0.2957183941569781
1106	98-99	-0.2892829359547875
1106	100	-0.3555654036908322
1107	1	-0.2734443068191439
1107	2	-0.3419579480569226
1107	3	-0.3133286388808294
1107	4	-0.1985000125234535
1107	5	-0.2345887822818753
1107	6	-0.42675722539125616
1107	7	-0.26498234011789634
1107	8	-0.32872862584272156
1107	9	-0.33831461031005006
1107	10-11	-0.3533498002103457
1107	12-13	-0.2975751376145652
1107	14-15	-0.37407338419748015
1107	16-17	-0.35579075801343407
1107	18-19	-0.37589922527519803
1107	20-21	-0.38042121310780885
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2216	64-65	0.20634018844263835
2216	66-67	0.1435680770110892
2216	68-69	0.17516024440411115
2216	70-71	0.13172900014733813
2216	72-73	0.19864654211745858
2216	74-75	0.16088972854841188
2216	76-77	0.19192297113025347
2216	78-79	0.13795536859016977
2216	80-81	0.16897870878546684
2216	82-83	0.12575036971389153
2216	84-85	0.12869051262126519
2216	86-87	0.07576916792885413
2216	88-89	0.06779957964772976
2216	90-91	0.11255887909624462
2216	92-93	0.11807695985840283
2216	94-95	0.04459554082012929
2216	96-97	0.06044167383538124
2216	98-99	0.07332994981133112
2216	100	0.19324909309085214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89147.0
3	18367.0
4	13689.0
5	11925.0
6	7939.0
7	6550.0
8	6981.0
9	7140.0
10	7287.0
11	7717.0
12	8783.0
13	9690.0
14	10236.0
15	10831.0
16	10999.0
17	10712.0
18	10613.0
19	10250.0
20	10090.0
21	10496.0
22	12620.0
23	19032.0
24	30876.0
25	41492.0
26	47648.0
27	36943.0
28	31569.0
29	38985.0
30	46943.0
31	51406.0
32	64514.0
33	86356.0
34	121843.0
35	192170.0
36	316376.0
37	591593.0
38	974253.0
39	540600.0
40	2677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72604740291111	22.344481830474567	14.363222370952162	21.56624839566216
2	14.655168251651135	23.302324722775637	40.17562868052706	21.866878345046175
3	16.767866014090178	29.753403459458294	30.965352869347317	22.51337765710421
4	11.26765963646774	20.298623696482174	39.707147468313266	28.72656919873682
5	11.429182551222812	41.13632535358376	34.83795977392682	12.59653232126661
6	26.261936160409284	41.661370533373585	19.17057445644649	12.906118849770639
7	23.827295982640393	36.53628992902264	23.36433722305323	16.27207686528374
8	23.396983077043537	37.808361958128934	21.470809517162962	17.323845447664567
9	24.411949430402693	19.487013499107263	21.84579711000073	34.255239960489305
10-11	21.402286311706973	30.31027705410928	29.586876511221966	18.70056012296178
12-13	22.444224253433077	28.763269680477936	28.83547404862943	19.957032017459557
14-15	20.473423529238204	30.271532456567403	26.482790943641177	22.772253070553216
16-17	19.388195697034654	33.225014028695135	27.221101313562475	20.16568896070774
18-19	19.569012585656385	32.00610593637905	29.082862652036667	19.3420188259279
20-21	21.524714185422248	30.91667348756556	28.20253260803417	19.35607971897802
22-23	20.989455332869472	31.024627865525883	28.360494699295767	19.625422102308875
24-25	20.5619607088811	31.024002728124362	28.339015980067895	20.07502058292664
26-27	20.176312891830463	31.08635399386548	28.390662543695917	20.346670570608143
28-29	20.238632425576668	30.9835033760536	28.57529105243306	20.202573145936668
30-31	20.48988651320409	31.353085825683895	28.21922565455855	19.937802006553458
32-33	20.086881929463722	31.124600795791153	28.671391366308637	20.117125908436496
34-35	20.49672841016693	30.76878991960223	28.497310026137257	20.237171644093582
36-37	20.640791390248843	30.975764326235673	28.107445685768027	20.275998597747456
38-39	20.37028735247352	30.97939764820176	28.467985945049044	20.182329054275673
40-41	20.23242508872234	30.72767145503224	28.65112106427375	20.38878239197167
42-43	20.162825309081665	30.77050208489767	28.608842043022392	20.457830562998268
44-45	20.43199007989149	30.86017782986276	28.434035960393345	20.273796129852403
46-47	20.478404171271944	31.080240437725926	28.229344485022434	20.212010905979692
48-49	20.388947166664444	30.764044809918765	28.404248889640492	20.442759133776295
50-51	20.325937502305344	30.723881219537525	28.342934918616354	20.60724635954078
52-53	20.32061603676019	30.752341135463112	28.434528361042034	20.49251446673467
54-55	20.475157672764745	31.078629821688192	28.24290344876898	20.203309056778085
56-57	20.448953263754724	30.821893614265566	28.0425557691181	20.68659735286161
58-59	20.399081247721206	30.502476346668672	28.44385268752066	20.65458971808946
60-61	20.616294605680803	30.83886159240532	28.088431527377043	20.45641227453683
62-63	20.330488405854254	30.80249878502125	28.285570648496506	20.581442160627994
64-65	20.391048812087558	30.63419497456414	28.41952086299645	20.555235350351857
66-67	20.278620730353783	30.662721497295614	28.226634262485216	20.832023509865383
68-69	20.731132972110487	30.89663953807054	28.031057447823837	20.341170041995134
70-71	20.59490224826716	30.59695056354203	28.123765904231902	20.68438128395891
72-73	20.436694081798297	30.675793254862	28.42366917555169	20.463843487788015
74-75	20.425551291446332	30.829736643734666	28.29681888373394	20.447893181085057
76-77	20.52624672567602	30.782511120111387	28.139446303214637	20.551795850997955
78-79	20.590394838268917	30.70681863382197	28.31957191359627	20.38321461431284
80-81	20.493544513687834	30.68587555937901	28.26410350114798	20.55647642578517
82-83	20.32307938067136	30.659542611119008	28.577385010825967	20.439992997383666
84-85	20.388664932551823	30.61427246978008	28.29014242960981	20.706920168058286
86-87	20.605787710007604	30.599884720762788	28.214841570493054	20.57948599873655
88-89	20.450531890142244	30.498888892672028	28.357609723267313	20.69296949391842
90-91	20.347519509349507	30.48084758420004	28.305296918907384	20.86633598754307
92-93	20.521839438841813	30.57687405588119	28.194215726563478	20.70707077871352
94-95	20.398911877975088	30.52177456888548	28.368595732765243	20.710717820374185
96-97	20.38359153776777	30.658526987841267	28.23197176797149	20.725909706419472
98-99	20.296585637680682	30.554398174255233	28.25422874498206	20.894787443082024
100	20.378772530479033	30.745786450698674	28.056231354061858	20.819209664760432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25446.0
1	18802.5
2	10628.5
3	7688.5
4	5238.5
5	3684.0
6	2962.0
7	2819.5
8	3098.0
9	3147.5
10	2553.5
11	2039.0
12	1927.5
13	1916.0
14	1982.0
15	2133.0
16	2394.0
17	2859.0
18	3532.5
19	4443.5
20	5922.0
21	8468.0
22	12844.0
23	19447.5
24	26048.0
25	32283.5
26	41174.5
27	52179.5
28	66425.5
29	79348.0
30	85954.5
31	93665.5
32	102963.5
33	110912.0
34	119138.0
35	124767.5
36	125854.0
37	125905.0
38	125052.5
39	123351.5
40	122254.5
41	118451.5
42	112447.5
43	108753.5
44	106673.0
45	103321.0
46	100091.0
47	96858.5
48	93105.0
49	90895.5
50	89600.0
51	85251.0
52	78483.0
53	72927.5
54	69430.0
55	67757.0
56	63311.0
57	56655.5
58	51409.0
59	46290.5
60	40524.0
61	34267.5
62	28591.5
63	23565.5
64	18807.0
65	15057.0
66	12083.5
67	9397.0
68	7133.0
69	5475.0
70	4265.0
71	3318.0
72	2661.0
73	2106.5
74	1764.5
75	1400.5
76	1003.5
77	809.0
78	633.5
79	473.5
80	344.0
81	242.0
82	189.0
83	147.5
84	120.0
85	101.0
86	77.5
87	71.0
88	66.5
89	53.5
90	48.5
91	41.5
92	33.5
93	31.5
94	23.0
95	19.5
96	23.5
97	24.5
98	22.0
99	24.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18186519125754322
2	0.1778394925578439
3	0.19076708838223047
4	0.18101469153225463
5	0.19518968695373112
6	0.07807587478149244
7	0.08034387404892868
8	0.06866367782163206
9	0.06282357970798376
10-11	0.07556690059189111
12-13	0.07429115100395822
14-15	0.07274607650301729
16-17	0.06860697783994617
18-19	0.07290200145265353
20-21	0.07460300090323072
22-23	0.07287365146181057
24-25	0.072916176448075
26-27	0.07640322532175822
28-29	0.07332725131529783
30-31	0.0790114244793099
32-33	0.07568030055526292
34-35	0.07585040050032064
36-37	0.08529094745102397
38-39	0.08181807357276223
40-41	0.07706945010656761
42-43	0.08007454913592063
44-45	0.09023802085311927
46-47	0.08078329890699446
48-49	0.0858721222633045
50-51	0.08278197326142264
52-53	0.08144952369180385
54-55	0.08363247298671123
56-57	0.07766479991426962
58-59	0.0848515225929582
60-61	0.08139282371011794
62-63	0.0732847263290334
64-65	0.07621895038127903
66-67	0.07626147536754346
68-69	0.07495737578876763
70-71	0.07059147719895285
72-73	0.06801162803224416
74-75	0.07283112647554615
76-77	0.07811839976775688
78-79	0.07453212592612332
80-81	0.08204487349950586
82-83	0.08337732306912465
84-85	0.08407189784477699
86-87	0.08086834887952332
88-89	0.08295207320648036
90-91	0.0814778736826468
92-93	0.07247675159000924
94-95	0.07227830165410856
96-97	0.07220742667700118
98-99	0.08103844882458103
100	0.08822517150326961
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3527338.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.1693505467264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65125229739132	49.82830429063598
2	14.37067289322353	18.44313492955199
3	4.1465426310151985	7.982428429396784
4	1.5094342671298424	3.874376664587836
5	0.7690079851438798	2.46733714859647
6	0.39869035753459564	1.5350220787342292
7	0.24256093945330087	1.0895484566905498
8	0.15768047105733185	0.8094602737320701
9	0.11934430179615335	0.6892421703940507
>10	0.5696124315221696	6.508009456375221
>50	0.04024110157104386	1.797652762462798
>100	0.0225757858665618	2.802596568901021
>500	0.001501375342794281	0.6188007998826922
>1k	8.39003854477201E-4	0.7622657795703709
>5k	0.0	0.0
>10k+	4.4158097604063124E-5	0.7918201904878783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27944	0.7922121441154775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.8349990842952956E-5	0.0
4	0.0	0.0	0.0	2.8349990842952956E-5	0.0
5	0.0	2.8349990842952956E-5	0.0	5.669998168590591E-5	0.0
6	0.0	2.8349990842952956E-5	0.0	5.669998168590591E-5	0.0
7	0.0	2.8349990842952956E-5	0.0	8.504997252885887E-5	0.0
8	0.0	2.8349990842952956E-5	0.0	8.504997252885887E-5	0.0
9	0.0	2.8349990842952956E-5	0.0	8.504997252885887E-5	0.0
10-11	0.0	2.8349990842952956E-5	0.0	1.1339996337181182E-4	0.0
12-13	0.0	2.8349990842952956E-5	0.0	1.1339996337181182E-4	0.0
14-15	0.0	2.8349990842952956E-5	0.0	1.7009994505771774E-4	0.0
16-17	0.0	2.8349990842952956E-5	0.0	2.2679992674362365E-4	0.0
18-19	0.0	2.8349990842952956E-5	0.0	2.834999084295296E-4	0.0
20-21	0.0	2.8349990842952956E-5	0.0	3.401998901154355E-4	0.0
22-23	0.0	2.8349990842952956E-5	0.0	4.819498443302003E-4	0.0
24-25	0.0	2.8349990842952956E-5	0.0	8.504997252885887E-4	0.0
26-27	0.0	2.8349990842952956E-5	0.0	0.0013749745558832185	0.0
28-29	0.0	2.8349990842952956E-5	0.0	0.0030901490018818726	0.0
30-31	0.0	2.8349990842952956E-5	0.0	0.00737099761916777	0.0
32-33	0.0	2.8349990842952956E-5	0.0	0.013919845503889902	1.4174995421476478E-5
34-35	0.0	4.2524986264429434E-5	0.0	0.024579442060840215	2.8349990842952956E-5
36-37	0.0	5.669998168590591E-5	0.0	0.040497961919158304	2.8349990842952956E-5
38-39	0.0	5.669998168590591E-5	0.0	0.06251172980871128	2.8349990842952956E-5
40-41	0.0	5.669998168590591E-5	0.0	0.08711952186039444	2.8349990842952956E-5
42-43	0.0	5.669998168590591E-5	0.0	0.11243606368315143	2.8349990842952956E-5
44-45	0.0	5.669998168590591E-5	0.0	0.14359270361955673	2.8349990842952956E-5
46-47	0.0	5.669998168590591E-5	0.0	0.18363706568522778	2.8349990842952956E-5
48-49	0.0	5.669998168590591E-5	0.0	0.22694167669783843	2.8349990842952956E-5
50-51	0.0	5.669998168590591E-5	0.0	0.27481063623616453	2.8349990842952956E-5
52-53	0.0	5.669998168590591E-5	0.0	0.3196461467542946	2.8349990842952956E-5
54-55	0.0	5.669998168590591E-5	0.0	0.3692869807203052	2.8349990842952956E-5
56-57	0.0	5.669998168590591E-5	0.0	0.4319121104923883	2.8349990842952956E-5
58-59	0.0	5.669998168590591E-5	0.0	0.5029571875448284	2.8349990842952956E-5
60-61	0.0	5.669998168590591E-5	0.0	0.578566613122984	2.8349990842952956E-5
62-63	0.0	5.669998168590591E-5	0.0	0.6579891124695166	2.8349990842952956E-5
64-65	0.0	5.669998168590591E-5	0.0	0.7442014346229366	2.8349990842952956E-5
66-67	0.0	5.669998168590591E-5	0.0	0.8439367024084452	2.8349990842952956E-5
68-69	0.0	5.669998168590591E-5	0.0	0.9588675652857764	2.8349990842952956E-5
70-71	0.0	5.669998168590591E-5	0.0	1.0888664482961372	2.8349990842952956E-5
72-73	0.0	5.669998168590591E-5	0.0	1.228419278220573	2.8349990842952956E-5
74-75	0.0	5.669998168590591E-5	0.0	1.3723521817302453	2.8349990842952956E-5
76-77	0.0	5.669998168590591E-5	0.0	1.5320335051531777	2.8349990842952956E-5
78-79	0.0	5.669998168590591E-5	0.0	1.7164218455957438	2.8349990842952956E-5
80-81	0.0	5.669998168590591E-5	0.0	1.9221577291430534	2.8349990842952956E-5
82-83	0.0	5.669998168590591E-5	0.0	2.144804382228185	2.8349990842952956E-5
84-85	0.0	5.669998168590591E-5	0.0	2.3648570111511855	2.8349990842952956E-5
86-87	0.0	5.669998168590591E-5	0.0	2.603408009099213	2.8349990842952956E-5
88	0.0	5.669998168590591E-5	0.0	2.799079645897274	2.8349990842952956E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4955	0.0	52.410828	1
GTATCAA	9035	0.0	38.94924	1
ATCAACG	10260	0.0	33.33262	3
TCAACGC	10305	0.0	33.322117	4
CAACGCA	11005	0.0	31.116655	5
TATCAAC	11405	0.0	30.357426	2
AACGCAG	12245	0.0	28.05535	6
TACCTGG	2695	0.0	26.706383	2
GTACATG	19075	0.0	25.94638	1
CGCAGAG	13005	0.0	25.728497	8
TACATGG	19025	0.0	25.04766	2
ACGCAGA	13615	0.0	24.852253	7
CATGGGT	8880	0.0	23.837315	4
ACATGGG	19395	0.0	23.74517	3
GTACCTG	3095	0.0	23.713165	1
TCTGTCG	635	0.0	23.682144	8
GTGGTAT	2425	0.0	21.728605	1
GCAGAGT	14650	0.0	21.684721	9
ATGGGTG	4700	0.0	21.517519	5
CTGTCGC	725	0.0	20.094095	9
>>END_MODULE