FastQCFastQC Report
Wed 25 May 2016
SRR2431427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527338
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339210.9616600393838073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272950.7738130000584009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175330.4970603894494942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92430.2620389653614142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.16340934721878084No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.1581362489219916No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.15161575102811242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40740.11549786269419034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110250.066.280081
GTATCAA227250.047.033381
GTGGTAT32550.040.5943831
TGGTATC33100.039.4919852
ATCAACG291050.036.4445723
TATCAAC293700.036.2303662
TCAACGC294600.036.0213664
TATAACG2950.035.0665322
CAACGCA303600.034.9535375
AACGCAG318650.033.4954646
CGCAGAG353450.029.865148
ACGCAGA357950.029.6209877
GCAGAGT376400.027.881879
GTACATG178350.025.3636991
ATAACGC4450.025.357863
CATGGGT84800.025.2831044
TACATGG179100.024.6788232
TACCTGG25000.024.2628522
ACATGGG181150.023.9046653
GAGTACT275150.023.82799512-13
Produced by FastQC (version 0.11.5)