##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431427_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3527338
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63067077779334	34.0	31.0	34.0	31.0	34.0
2	32.74731624811685	34.0	31.0	34.0	31.0	34.0
3	32.85389492019194	34.0	31.0	34.0	31.0	34.0
4	36.32544825588021	37.0	37.0	37.0	35.0	37.0
5	36.230255223627566	37.0	37.0	37.0	35.0	37.0
6	36.27409706696665	37.0	37.0	37.0	35.0	37.0
7	36.23463728171216	37.0	37.0	37.0	35.0	37.0
8	36.2498368458027	37.0	37.0	37.0	35.0	37.0
9	38.04263328322945	39.0	39.0	39.0	35.0	39.0
10-11	38.02423045367357	39.0	39.0	39.0	35.0	39.0
12-13	38.006331828704816	39.0	39.0	39.0	35.0	39.0
14-15	39.45598819846582	41.0	39.0	41.0	36.0	41.0
16-17	39.49003923071733	41.0	39.0	41.0	36.5	41.0
18-19	39.57482087058286	41.0	40.0	41.0	37.0	41.0
20-21	39.56852079386778	41.0	40.0	41.0	37.0	41.0
22-23	39.49805547412808	41.0	39.5	41.0	36.0	41.0
24-25	39.44279765647636	41.0	39.0	41.0	36.0	41.0
26-27	39.318782606033224	41.0	39.0	41.0	35.5	41.0
28-29	39.22985889075558	41.0	39.0	41.0	35.0	41.0
30-31	39.02123272564183	41.0	39.0	41.0	35.0	41.0
32-33	38.76311669026331	41.0	38.0	41.0	35.0	41.0
34-35	38.608611791668395	41.0	38.0	41.0	35.0	41.0
36-37	38.452463018854445	41.0	38.0	41.0	35.0	41.0
38-39	38.29720627850237	40.5	38.0	41.0	35.0	41.0
40-41	38.14460323904316	40.0	38.0	41.0	34.0	41.0
42-43	37.95491288331314	40.0	38.0	41.0	34.0	41.0
44-45	37.68275594796983	40.0	38.0	41.0	33.0	41.0
46-47	37.28076711106222	40.0	37.5	41.0	32.5	41.0
48-49	36.916856706105285	40.0	37.0	41.0	31.0	41.0
50-51	36.74758160403114	40.0	37.0	41.0	31.0	41.0
52-53	36.692373540613346	40.0	37.0	41.0	31.0	41.0
54-55	36.56052056820185	40.0	36.0	41.0	31.0	41.0
56-57	36.41497015596464	40.0	36.0	41.0	30.5	41.0
58-59	36.24344293061793	40.0	35.5	41.0	30.0	41.0
60-61	36.09915409297322	40.0	35.0	41.0	30.0	41.0
62-63	35.94449681317753	39.0	35.0	41.0	30.0	41.0
64-65	35.71638272260839	39.0	35.0	41.0	29.5	41.0
66-67	35.458363077198726	39.0	35.0	41.0	29.0	41.0
68-69	35.16936667254457	38.0	35.0	41.0	29.0	41.0
70-71	34.87555346269623	37.0	35.0	40.0	28.5	41.0
72-73	34.56042020923427	37.0	35.0	39.5	28.0	41.0
74-75	34.226830828233645	36.0	35.0	39.0	28.0	41.0
76-77	33.89163201825286	36.0	35.0	39.0	28.0	41.0
78-79	33.56823289971077	35.5	34.0	38.5	27.0	40.0
80-81	33.2956062050192	35.0	34.0	37.0	27.0	39.0
82-83	33.0822919436697	35.0	34.0	37.0	28.0	39.0
84-85	32.80938585981836	35.0	34.0	36.5	27.0	38.5
86-87	32.557488678431156	35.0	34.0	36.0	27.0	37.0
88-89	32.31633401732411	35.0	34.0	36.0	26.0	37.0
90-91	32.12796930716591	35.0	34.0	35.5	26.0	37.0
92-93	31.95839383693879	35.0	34.0	35.0	26.0	36.0
94-95	31.814977470262278	35.0	34.0	35.0	26.0	36.0
96-97	31.667408680426995	35.0	34.0	35.0	25.0	36.0
98-99	31.54893917169265	35.0	34.0	35.0	25.0	36.0
100	31.44373008767518	35.0	34.0	35.0	24.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-1.5524025351254522E-4
1101	2	0.029967999899362496
1101	3	0.06958252340198356
1101	4	0.041882113071466165
1101	5	0.027937287399019795
1101	6	0.03881450036040235
1101	7	0.06447053291709892
1101	8	0.07980489773832744
1101	9	0.05837222760714411
1101	10-11	0.08186368201710792
1101	12-13	0.051823406771916325
1101	14-15	0.10449696106973505
1101	16-17	0.07781617547492203
1101	18-19	0.07343385284942627
1101	20-21	0.08747488800845815
1101	22-23	0.05433631633728453
1101	24-25	0.018908275026063848
1101	26-27	0.09758908043762915
1101	28-29	-0.002580159343231969
1101	30-31	-0.013412615561080088
1101	32-33	0.014963627776992894
1101	34-35	0.032915828048842855
1101	36-37	0.006442877179544837
1101	38-39	-0.06106117009392875
1101	40-41	-0.08659779864574091
1101	42-43	-0.08469919462012143
1101	44-45	-0.08188078527572884
1101	46-47	-0.048226961899821674
1101	48-49	0.026400389612916797
1101	50-51	-0.005593304479255323
1101	52-53	0.0029982356224635964
1101	54-55	0.050818097214026636
1101	56-57	0.07245595929438053
1101	58-59	0.09726341060289201
1101	60-61	0.12589582155427337
1101	62-63	0.0425183276093648
1101	64-65	0.03658209185822159
1101	66-67	0.017747627813733402
1101	68-69	-0.010172403978003786
1101	70-71	6.715160948687071E-4
1101	72-73	-0.040825430906664906
1101	74-75	0.011848154161647528
1101	76-77	0.00424722948591949
1101	78-79	0.07040629785436892
1101	80-81	0.12316593466618286
1101	82-83	0.08285750944723702
1101	84-85	0.1393870444075631
1101	86-87	0.11731344057982085
1101	88-89	0.13408051108557117
1101	90-91	0.06338833266718069
1101	92-93	0.0560778304932974
1101	94-95	0.06447111308835929
1101	96-97	0.06172810649208671
1101	98-99	0.11033363269923058
1101	100	0.17327383938461693
1102	1	-0.021051699211355412
1102	2	-0.02610746402837094
1102	3	-0.020971112253249657
1102	4	0.012074510456571375
1102	5	0.028134953228914128
1102	6	-0.02639412434230337
1102	7	-0.02566246794844318
1102	8	-0.008359694124564498
1102	9	0.0181861377608854
1102	10-11	-0.007934548128105234
1102	12-13	-0.028029007765368874
1102	14-15	-0.09320509830362766
1102	16-17	-0.10138852256276465
1102	18-19	-0.11578356734814577
1102	20-21	-0.055305141492297594
1102	22-23	-0.003271156922295404
1102	24-25	-0.010155661461972443
1102	26-27	0.00804314788007332
1102	28-29	-0.010583860580375415
1102	30-31	-0.01764682466297529
1102	32-33	0.036753429996949194
1102	34-35	-0.06034722551066096
1102	36-37	-0.07237526200765387
1102	38-39	-0.10500112600460909
1102	40-41	-0.11897949897799265
1102	42-43	-0.05093068050241101
1102	44-45	-0.03581391885975904
1102	46-47	0.012068036900863888
1102	48-49	-0.006021545868705402
1102	50-51	-0.07591974499674592
1102	52-53	-0.02495662660794551
1102	54-55	-0.10335361553659794
1102	56-57	-0.03460695729508245
1102	58-59	-0.0952805581462357
1102	60-61	-0.15159591011262563
1102	62-63	-0.19351708122575673
1102	64-65	-0.18662578025484322
1102	66-67	-0.18359022957116622
1102	68-69	-0.20049778795100792
1102	70-71	-0.2371468710471376
1102	72-73	-0.1986223722579794
1102	74-75	-0.1663616906437042
1102	76-77	-0.18222981811632621
1102	78-79	-0.11413109430194623
1102	80-81	-0.1424772693097225
1102	82-83	-0.1289288946972107
1102	84-85	-0.12493347612937811
1102	86-87	-0.11086683367120287
1102	88-89	-0.10805116735431852
1102	90-91	-0.11631885856165525
1102	92-93	-0.1325924590950791
1102	94-95	-0.1074260965554501
1102	96-97	-0.09198864567113318
1102	98-99	-0.11578433731394355
1102	100	-0.06931900644492117
1103	1	-0.10312420908437758
1103	2	-0.10214996992252878
1103	3	-0.01981668533338876
1103	4	0.013124445830904108
1103	5	-0.01935255412333703
1103	6	-0.02433394956880619
1103	7	-0.0617544501646492
1103	8	-0.017331138971563576
1103	9	-0.028322330919756666
1103	10-11	-0.00857223230507742
1103	12-13	-0.013343234617522626
1103	14-15	-0.03509416797824372
1103	16-17	0.0035444669241400106
1103	18-19	-0.03363834587858605
1103	20-21	-0.0471049492834581
1103	22-23	-0.020607679011177993
1103	24-25	-0.06709436346227449
1103	26-27	-0.03355865885829701
1103	28-29	-0.0196124166601237
1103	30-31	0.0057598607570810145
1103	32-33	-0.033033793397791555
1103	34-35	-0.02002043959337385
1103	36-37	-0.034486303234629645
1103	38-39	-0.017058477266722605
1103	40-41	-0.02456771918964762
1103	42-43	-0.006303252715099461
1103	44-45	-0.011573386272601738
1103	46-47	-0.03136179266284955
1103	48-49	-0.06019230312580248
1103	50-51	-0.10495727360256524
1103	52-53	-0.004932517095241451
1103	54-55	-0.024085966119095303
1103	56-57	-0.05614067230701636
1103	58-59	0.055970065868315544
1103	60-61	-0.011422471137535695
1103	62-63	-0.10583384645789096
1103	64-65	-0.08033262439701616
1103	66-67	-0.06542206907955972
1103	68-69	-0.0847724359079507
1103	70-71	-0.13193581666025977
1103	72-73	-0.19312633320578954
1103	74-75	-0.11776157615866367
1103	76-77	-0.09942115642365934
1103	78-79	-0.06775260290964269
1103	80-81	-0.09763471868421902
1103	82-83	-0.1189415726958174
1103	84-85	-0.13560068824241966
1103	86-87	-0.0905883301075221
1103	88-89	-0.09153872289779486
1103	90-91	-0.15286958414847618
1103	92-93	-0.15298846737902494
1103	94-95	-0.20322368291659387
1103	96-97	-0.13003746955816453
1103	98-99	-0.15334445277516906
1103	100	-0.12705657063809994
1104	1	-0.1001681278426787
1104	2	-0.06807213726459338
1104	3	-0.0697278229882059
1104	4	-0.018978012989386173
1104	5	-0.04046096496732332
1104	6	-0.03285994983976792
1104	7	-0.03407346640223352
1104	8	-0.0805612066755188
1104	9	-0.05134807342412273
1104	10-11	-0.05205635891065441
1104	12-13	-0.02901791255000319
1104	14-15	-0.07739208135816966
1104	16-17	-0.037739343880275555
1104	18-19	-0.042589373875536296
1104	20-21	-0.011211054491333527
1104	22-23	-0.03011003162113468
1104	24-25	-0.016957409027362758
1104	26-27	-0.03689779564864892
1104	28-29	-0.04101472783420945
1104	30-31	-0.052836664943164635
1104	32-33	0.012948276818335103
1104	34-35	8.215705890322056E-4
1104	36-37	0.03202991780428732
1104	38-39	0.08871585976875451
1104	40-41	-0.037869038800984356
1104	42-43	-0.031105633555007728
1104	44-45	0.0273271959976924
1104	46-47	0.1061444993602052
1104	48-49	0.13084648677315158
1104	50-51	0.06512475300205267
1104	52-53	0.09599098110906112
1104	54-55	0.14124221218647648
1104	56-57	0.14532129070680355
1104	58-59	0.13385574529612398
1104	60-61	0.08863977015924007
1104	62-63	0.10552164529438102
1104	64-65	0.07650931975575759
1104	66-67	0.15631175192977764
1104	68-69	0.09165484443263949
1104	70-71	0.11176335507102664
1104	72-73	0.07532577321176603
1104	74-75	0.021897563358074024
1104	76-77	0.04686043957342889
1104	78-79	0.10068332320502549
1104	80-81	0.08702740015344546
1104	82-83	0.07431759673203686
1104	84-85	0.03928295925274483
1104	86-87	0.039843130182688924
1104	88-89	0.08753092185746425
1104	90-91	0.1300935800645604
1104	92-93	0.16501522708543348
1104	94-95	0.15610638407990507
1104	96-97	0.20021531135057202
1104	98-99	0.1774106630189749
1104	100	0.22744952960994524
1105	1	-0.12240974987130926
1105	2	-0.07872298664428712
1105	3	-0.05546764101267598
1105	4	-0.008099655413168705
1105	5	-0.016782518763925225
1105	6	-0.016717764670644897
1105	7	-0.004597098858710069
1105	8	-0.008840846296180871
1105	9	0.043250954635738026
1105	10-11	-0.037607148006166824
1105	12-13	-0.027597887583503677
1105	14-15	0.019610009121684868
1105	16-17	-0.04477143948653861
1105	18-19	-0.04686301300061757
1105	20-21	0.023077624087108006
1105	22-23	-0.06497535059023818
1105	24-25	-0.11781858789969846
1105	26-27	-0.022722915058288606
1105	28-29	-0.04501307182576397
1105	30-31	-0.03350550574519673
1105	32-33	-0.10646021170809661
1105	34-35	-0.06779644140855368
1105	36-37	-0.07473154785399316
1105	38-39	-0.00812697875714008
1105	40-41	-0.054599025996878936
1105	42-43	-0.09443840275276472
1105	44-45	-0.05691722007223632
1105	46-47	-0.03195971074462278
1105	48-49	-0.025926921835768724
1105	50-51	0.03368991559852219
1105	52-53	-0.003730367699368742
1105	54-55	0.031670624914418966
1105	56-57	0.08591365273488805
1105	58-59	0.015498909908266967
1105	60-61	0.043217019029334836
1105	62-63	0.021983893216862782
1105	64-65	0.015994674497342487
1105	66-67	-0.06700557183376077
1105	68-69	0.06812986126685416
1105	70-71	0.08706543529353183
1105	72-73	0.014019730116579865
1105	74-75	-0.0029378715599932548
1105	76-77	-0.0920797014044723
1105	78-79	0.0057957195564668496
1105	80-81	0.010497623197366579
1105	82-83	-0.035969035218542444
1105	84-85	-0.050044806429468736
1105	86-87	-0.03994960920707058
1105	88-89	0.006978926359344939
1105	90-91	0.07059212759516953
1105	92-93	0.02795487589192902
1105	94-95	0.042824328437973236
1105	96-97	0.03479706422460538
1105	98-99	0.06390306665536727
1105	100	0.07936223865367253
1106	1	-0.15517680708713044
1106	2	-0.11872039911728649
1106	3	-0.12147713401161297
1106	4	-0.019507742411477125
1106	5	-0.035073923199689716
1106	6	-0.06630951719092337
1106	7	-0.04965013816918429
1106	8	-0.039807064607344955
1106	9	-0.07197906434648615
1106	10-11	-0.03597513081398063
1106	12-13	-0.09618628989247213
1106	14-15	-0.13519387937365934
1106	16-17	-0.13889358488965087
1106	18-19	-0.1499449514666651
1106	20-21	-0.0686265002630222
1106	22-23	-0.12732413643313834
1106	24-25	-0.08778245165744636
1106	26-27	-0.07008906518241531
1106	28-29	-0.0891522473433497
1106	30-31	-0.1760600465869402
1106	32-33	-0.15796498108328194
1106	34-35	-0.18540568333304464
1106	36-37	-0.1840996864090343
1106	38-39	-0.2294731222970583
1106	40-41	-0.20566849462439052
1106	42-43	-0.2035829066305226
1106	44-45	-0.18837446564529614
1106	46-47	-0.30377304234504976
1106	48-49	-0.31256549605660666
1106	50-51	-0.3281960594540365
1106	52-53	-0.34667347032547013
1106	54-55	-0.338186993416393
1106	56-57	-0.26051821003416364
1106	58-59	-0.2569565137809704
1106	60-61	-0.31942590753324396
1106	62-63	-0.33700161792642547
1106	64-65	-0.3130070723052967
1106	66-67	-0.29164561579656834
1106	68-69	-0.31121313692612773
1106	70-71	-0.3522555400710061
1106	72-73	-0.34553987471079495
1106	74-75	-0.3353863256439098
1106	76-77	-0.3693284855793024
1106	78-79	-0.36856063032762876
1106	80-81	-0.31977743395587765
1106	82-83	-0.29150763196003027
1106	84-85	-0.26983604818316564
1106	86-87	-0.3184651942306118
1106	88-89	-0.31185660586030295
1106	90-91	-0.2925046333025705
1106	92-93	-0.24818466696868313
1106	94-95	-0.28747743246735524
1106	96-97	-0.26109145670893597
1106	98-99	-0.2725890086363414
1106	100	-0.21293292627694882
1107	1	-0.26818327681935017
1107	2	-0.25974510279583995
1107	3	-0.1814508255569649
1107	4	-0.20164210002449323
1107	5	-0.22631847958188445
1107	6	-0.23681395955780715
1107	7	-0.17648571681375813
1107	8	-0.20350590144645508
1107	9	-0.16887716385701879
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2216	34-35	-0.0757203101209214
2216	36-37	-0.011599357708554692
2216	38-39	-0.027313456999628727
2216	40-41	-0.015151046377766875
2216	42-43	0.09878243967237665
2216	44-45	0.10130882039250366
2216	46-47	0.11314127973896859
2216	48-49	0.12879758206561576
2216	50-51	0.20290980980985296
2216	52-53	0.16265745175158486
2216	54-55	0.13186426616972113
2216	56-57	0.12244073394997201
2216	58-59	0.1190050161767644
2216	60-61	0.04397958857452977
2216	62-63	0.08272926924647805
2216	64-65	0.11518183819560335
2216	66-67	0.05014481716239061
2216	68-69	0.08545618391088539
2216	70-71	0.09239856679821656
2216	72-73	0.075860692953448
2216	74-75	0.06267109877119736
2216	76-77	0.01613291555142382
2216	78-79	0.04670971770573118
2216	80-81	0.017844281029269382
2216	82-83	0.045568499197059964
2216	84-85	0.1169302201319411
2216	86-87	0.09492066828828172
2216	88-89	0.0934328922921992
2216	90-91	0.08075686546546024
2216	92-93	0.13290015367288532
2216	94-95	0.09990565776519844
2216	96-97	0.1028559677238583
2216	98-99	0.0927844599584624
2216	100	0.13596888985950528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51.0
3	314.0
4	76.0
5	138.0
6	378.0
7	797.0
8	1375.0
9	2422.0
10	3580.0
11	5195.0
12	7892.0
13	11589.0
14	13127.0
15	17086.0
16	22140.0
17	33428.0
18	38347.0
19	19955.0
20	11936.0
21	10635.0
22	10098.0
23	10059.0
24	10934.0
25	12485.0
26	15122.0
27	19125.0
28	23783.0
29	29564.0
30	37429.0
31	47100.0
32	59902.0
33	77349.0
34	107499.0
35	183575.0
36	282060.0
37	572809.0
38	1085442.0
39	734498.0
40	8044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.892658530742196	21.194390379782003	15.049097643851772	21.863853445624024
2	14.326155116283488	22.191432918868482	40.99014543834924	22.49226652649879
3	16.04650463923722	28.987974951974653	32.829435739023346	22.136084669764784
4	10.755136026091064	19.112684976602754	40.386801605063084	29.7453773922431
5	10.739565251920174	39.188506177461626	35.93084533088277	14.14108323973543
6	26.015028897145665	40.94410572505385	18.95386832790053	14.086997049899953
7	23.075304946676503	36.05327870479098	24.018452442039862	16.852963906492658
8	24.519623580161586	34.95477325960824	21.604734221670846	18.92086893855933
9	25.54633298330525	15.83600201398449	22.94961409970584	35.66805090300442
10-11	22.1110083581443	27.762579032686972	30.205426868647123	19.920985740521605
12-13	24.235655896883145	26.41903044165317	30.195064946994023	19.150248714469665
14-15	21.390946940724138	27.282826312647103	29.507124069198927	21.819102677429836
16-17	18.49985739954606	30.605034164573965	30.06329418955598	20.831814246323997
18-19	18.475453295219594	27.78875395234422	34.689157854778266	19.04663489765792
20-21	20.222743043054	26.752057784085338	34.559517687275786	18.46568148558488
22-23	19.618749269101695	26.853412807818927	34.868353255110975	18.659484667968403
24-25	19.294385537871772	26.97251495958566	34.72557342833985	19.007526074202723
26-27	18.970440127206604	27.090017740034433	34.68582845535543	19.253713677403532
28-29	19.016087076516275	26.967090872725567	34.885974097438165	19.130847953319993
30-31	19.318206785556384	27.320689116032646	34.527669012019146	18.83343508639182
32-33	18.928710902200486	27.198396503834033	34.81100589191285	19.061886702052636
34-35	19.32365759639567	27.030675775399448	34.42188681406318	19.223779814141707
36-37	19.556932939339706	27.184936689517958	33.91040850236966	19.34772186877268
38-39	19.350197955663035	27.28747584912561	34.10435162255603	19.25797457265533
40-41	19.315312827664297	27.167589908921286	34.02113806363929	19.49595919977513
42-43	19.31763785003376	27.280098306915974	33.75332635636597	19.648937486684297
44-45	19.654892237279554	27.49207404007504	33.24542933712241	19.607604385523
46-47	19.85297390407994	27.85039008225195	32.52525349888995	19.771382514778164
48-49	20.013296166437385	27.811129231508513	32.05196844924599	20.123606152808108
50-51	19.973872555850033	27.952112726767066	31.757332857429798	20.3166818599531
52-53	19.94253144844649	28.16455153423115	31.675360943242897	20.21755607407946
54-55	20.097964170997162	28.487700258837474	31.49609759278317	19.918237977382198
56-57	19.956005592432554	28.137355087856275	31.434766837152793	20.47187248255838
58-59	19.870062879156322	27.816276457929906	31.860733420528863	20.45292724238491
60-61	20.09542438392247	28.049875605828884	31.59110145435788	20.263598555890766
62-63	19.90166424602593	27.90520122597917	31.847802697981997	20.345331830012903
64-65	19.88433319999195	27.801069207013775	31.942353996144913	20.372243596849362
66-67	19.8303447169232	27.85585070176329	31.761553855622765	20.552250725690744
68-69	20.296978546493655	28.06444996357722	31.34801423911686	20.290557250812267
70-71	20.204018307896014	27.813313779358072	31.364738858465348	20.617929054280566
72-73	20.06981201078436	28.004411268579915	31.526707169445444	20.399069551190276
74-75	20.095029721594386	28.122277056148192	31.309755926422838	20.47293729583458
76-77	20.17841324984588	28.13551038019979	31.15906950793897	20.52700686201536
78-79	20.193884263945574	28.126528696580582	31.34602128165354	20.3335657578203
80-81	20.13948276558603	28.08390512053423	31.27026415966241	20.506347954217333
82-83	19.916028948027193	28.067662200439038	31.543251229627757	20.473057621906015
84-85	19.954857842461156	28.134722818562572	31.180549777216378	20.72986956175989
86-87	20.088331669158677	28.10980944491412	31.088157597056536	20.71370128887067
88-89	19.911844816258316	27.990681264223866	31.282488530541396	20.81498538897642
90-91	19.775352570872094	28.073259896720014	31.136150687281084	21.015236845126804
92-93	19.84128379140771	28.155145207300087	31.0749263884975	20.92864461279471
94-95	19.705291126116876	27.990241292837336	31.269849572400965	21.034618008644827
96-97	19.705380849301605	28.18675920493483	31.027998911925962	21.0798610338376
98-99	19.637901447753496	28.027781376662837	31.054605938150896	21.279711237432764
100	19.74421424495092	28.174167010883878	30.847636184566955	21.233982559598243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3390.0
1	2024.5
2	4141.5
3	6790.0
4	3715.5
5	1977.5
6	4408.0
7	10926.5
8	19281.5
9	21147.5
10	13404.0
11	6304.5
12	4995.5
13	5196.5
14	5716.5
15	6170.0
16	6663.0
17	7274.0
18	8008.0
19	8736.5
20	9970.5
21	12369.0
22	16342.5
23	22641.5
24	28853.5
25	34468.0
26	42525.5
27	52800.5
28	66733.0
29	79377.0
30	85820.0
31	93296.5
32	101796.0
33	109755.0
34	117746.5
35	122980.0
36	124071.0
37	123858.5
38	123287.5
39	121129.5
40	119878.5
41	116435.0
42	110898.5
43	107930.5
44	105599.5
45	101820.0
46	98620.5
47	96129.0
48	92680.0
49	89555.0
50	88311.5
51	84011.5
52	76415.0
53	70628.5
54	67055.0
55	64683.5
56	59410.0
57	52993.0
58	48368.5
59	43773.5
60	38298.5
61	32064.0
62	26953.5
63	22075.5
64	17276.5
65	13692.0
66	10707.5
67	8175.0
68	6186.5
69	4572.0
70	3344.5
71	2533.0
72	2013.5
73	1575.5
74	1334.0
75	1022.0
76	619.0
77	498.5
78	398.5
79	211.0
80	84.0
81	39.0
82	24.5
83	17.0
84	7.5
85	5.0
86	6.0
87	4.5
88	1.5
89	1.0
90	1.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005188048324260391
2	0.003940648727170461
3	9.638996886604005E-4
4	0.0
5	0.04587028518389789
6	0.0
7	0.0
8	0.0
9	5.669998168590591E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.8349990842952956E-5
20-21	0.0
22-23	1.4174995421476478E-5
24-25	2.8349990842952956E-5
26-27	1.5592494963624127E-4
28-29	9.922496795033535E-5
30-31	2.1262493132214718E-4
32-33	0.0012899245833543594
34-35	7.654497527597298E-4
36-37	0.0011056496428751652
38-39	9.355496978174475E-4
40-41	1.275749587932883E-4
42-43	4.961248397516767E-4
44-45	1.4174995421476478E-4
46-47	1.4174995421476478E-4
48-49	1.5592494963624127E-4
50-51	3.5437488553691194E-4
52-53	4.961248397516767E-4
54-55	7.937997436026828E-4
56-57	0.0020270243452711364
58-59	4.6777484890872376E-4
60-61	0.0012048746108255007
62-63	9.922496795033535E-4
64-65	0.0014458495329906008
66-67	2.551499175865766E-4
68-69	3.5437488553691194E-4
70-71	2.1262493132214718E-4
72-73	2.2679992674362365E-4
74-75	5.811748122805356E-4
76-77	6.095248031234886E-4
78-79	7.796247481812063E-4
80-81	5.811748122805356E-4
82-83	0.0014458495329906008
84-85	0.001034774665767783
86-87	0.0018143994139489892
88-89	0.00100642467492483
90-91	9.355496978174475E-4
92-93	0.0010205996703463063
94-95	7.512747573382533E-4
96-97	1.275749587932883E-4
98-99	3.1184989927248255E-4
100	3.1184989927248255E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3527338.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.40970470395616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16311629647146	44.65418543802484
2	15.778485537937565	18.747903329690267
3	4.687042438256131	8.353674215751221
4	1.7698824534845832	4.2059277568893005
5	0.8143109462811059	2.4188986427879806
6	0.4447752884656424	1.5854381128416437
7	0.2750646469495253	1.1439056614838476
8	0.18860603370303114	0.8964023014145185
9	0.1329105931567712	0.7106561182324289
>10	0.664013233656989	7.078076888376161
>50	0.04811405889494294	1.9706434255977556
>100	0.030136143107790513	3.441744258024389
>500	0.0021062500594784903	0.8534550300608958
>1k	0.0011009943407927004	0.9620352077890255
>5k	1.9147727665960008E-4	0.7375091357334989
>10k+	1.4360795749470006E-4	2.2395444773021747
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33921	0.9616600393838073	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27295	0.7738130000584009	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17533	0.4970603894494942	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9243	0.2620389653614142	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5764	0.16340934721878084	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5578	0.1581362489219916	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5348	0.15161575102811242	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4074	0.11549786269419034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.504997252885887E-5	0.0
4	0.0	0.0	0.0	8.504997252885887E-5	0.0
5	0.0	0.0	0.0	1.1339996337181182E-4	0.0
6	0.0	0.0	0.0	1.1339996337181182E-4	0.0
7	0.0	0.0	0.0	1.4174995421476478E-4	0.0
8	0.0	0.0	0.0	1.4174995421476478E-4	0.0
9	0.0	0.0	0.0	1.7009994505771774E-4	0.0
10-11	0.0	0.0	0.0	1.984499359006707E-4	0.0
12-13	0.0	0.0	0.0	1.984499359006707E-4	0.0
14-15	0.0	0.0	0.0	2.693249130080531E-4	0.0
16-17	0.0	0.0	0.0	3.1184989927248255E-4	2.8349990842952956E-5
18-19	0.0	0.0	0.0	3.54374885536912E-4	2.8349990842952956E-5
20-21	0.0	0.0	0.0	3.9689987180134144E-4	2.8349990842952956E-5
22-23	0.0	0.0	0.0	5.102998351731532E-4	2.8349990842952956E-5
24-25	0.0	0.0	0.0	8.788497161315418E-4	2.8349990842952956E-5
26-27	0.0	0.0	0.0	0.0014458495329906008	2.8349990842952956E-5
28-29	0.0	0.0	0.0	0.0033027739332040194	2.8349990842952956E-5
30-31	0.0	0.0	0.0	0.0077537224955476335	2.8349990842952956E-5
32-33	0.0	0.0	0.0	0.014330920371112721	2.8349990842952956E-5
34-35	0.0	0.0	0.0	0.02520314185938518	2.8349990842952956E-5
36-37	0.0	0.0	0.0	0.04139098663071132	2.8349990842952956E-5
38-39	0.0	0.0	0.0	0.06350397948821462	2.8349990842952956E-5
40-41	0.0	0.0	0.0	0.08845197143001324	2.8349990842952956E-5
42-43	0.0	0.0	0.0	0.11425046309710041	2.8349990842952956E-5
44-45	0.0	0.0	0.0	0.14580400290530707	2.8349990842952956E-5
46-47	0.0	0.0	0.0	0.18625943983820092	2.8349990842952956E-5
48-49	0.0	0.0	0.0	0.23024445063104246	2.8349990842952956E-5
50-51	0.0	0.0	0.0	0.2782976851098477	2.8349990842952956E-5
52-53	0.0	0.0	0.0	0.3236576704585724	4.2524986264429434E-5
54-55	0.0	0.0	0.0	0.37427657910866496	5.669998168590591E-5
56-57	0.0	0.0	0.0	0.43826250844120973	5.669998168590591E-5
58-59	0.0	0.0	0.0	0.509846235319666	8.504997252885887E-5
60-61	0.0	0.0	0.0	0.5865754855361182	8.504997252885887E-5
62-63	0.0	0.0	0.0	0.6671886844980549	8.504997252885887E-5
64-65	0.0	0.0	0.0	0.7549177311615728	8.504997252885887E-5
66-67	0.0	0.0	0.0	0.8561130234754935	8.504997252885887E-5
68-69	0.0	0.0	0.0	0.972688185821716	1.4174995421476478E-4
70-71	0.0	0.0	0.0	1.1045865182185546	1.4174995421476478E-4
72-73	0.0	0.0	0.0	1.2463931724150052	1.4174995421476478E-4
74-75	0.0	0.0	0.0	1.392636600178378	1.4174995421476478E-4
76-77	0.0	5.669998168590591E-5	0.0	1.554826897790912	1.4174995421476478E-4
78-79	0.0	5.669998168590591E-5	0.0	1.742489662175839	1.4174995421476478E-4
80-81	0.0	5.669998168590591E-5	0.0	1.9519677445144186	1.4174995421476478E-4
82-83	0.0	5.669998168590591E-5	0.0	2.1792354461069507	1.4174995421476478E-4
84-85	0.0	5.669998168590591E-5	0.0	2.404206798441204	1.4174995421476478E-4
86-87	0.0	5.669998168590591E-5	0.0	2.6482435196173433	1.4174995421476478E-4
88	0.0	5.669998168590591E-5	0.0	2.84809678006474	1.4174995421476478E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11025	0.0	66.28008	1
GTATCAA	22725	0.0	47.03338	1
GTGGTAT	3255	0.0	40.594383	1
TGGTATC	3310	0.0	39.491985	2
ATCAACG	29105	0.0	36.444572	3
TATCAAC	29370	0.0	36.230366	2
TCAACGC	29460	0.0	36.021366	4
TATAACG	295	0.0	35.066532	2
CAACGCA	30360	0.0	34.953537	5
AACGCAG	31865	0.0	33.495464	6
CGCAGAG	35345	0.0	29.86514	8
ACGCAGA	35795	0.0	29.620987	7
GCAGAGT	37640	0.0	27.88187	9
GTACATG	17835	0.0	25.363699	1
ATAACGC	445	0.0	25.35786	3
CATGGGT	8480	0.0	25.283104	4
TACATGG	17910	0.0	24.678823	2
TACCTGG	2500	0.0	24.262852	2
ACATGGG	18115	0.0	23.904665	3
GAGTACT	27515	0.0	23.827995	12-13
>>END_MODULE