FastQCFastQC Report
Wed 25 May 2016
SRR2431426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347118
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156640.3603306834551075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.048.659981
GTATCAA91750.038.1041831
TCAACGC102850.032.7962844
ATCAACG104050.032.421413
CAACGCA110750.030.5843035
TATCAAC115800.029.7008342
AACGCAG120300.028.399026
CGCAGAG131600.025.4956978
GTACATG191900.025.2307591
ACGCAGA135050.025.1233277
TACATGG190800.024.6346842
ACATGGG194800.023.4039783
GTGGTAT26400.023.1701761
CATGGGT83850.022.9472184
TGGTATC25550.022.097862
GCAGAGT149750.021.5274129
ATGGGTG50200.019.4925845
TATACCG2753.1746822E-818.8178425
CAGAGTA147100.018.33733710-11
AGAGTAC133650.018.24884810-11
Produced by FastQC (version 0.11.5)