##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431426_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4347118
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.893111482135982	33.0	31.0	34.0	27.0	34.0
2	30.911461800668857	33.0	31.0	34.0	27.0	34.0
3	31.098769115538158	34.0	31.0	34.0	27.0	34.0
4	34.33727149803617	37.0	35.0	37.0	30.0	37.0
5	34.39761469552931	37.0	35.0	37.0	31.0	37.0
6	34.4227828644173	37.0	35.0	37.0	31.0	37.0
7	34.36645865145598	37.0	35.0	37.0	31.0	37.0
8	34.326597069598755	37.0	35.0	37.0	31.0	37.0
9	35.796631469401106	39.0	37.0	39.0	30.0	39.0
10-11	35.810747603354685	39.0	37.0	39.0	30.0	39.0
12-13	35.74523327869177	39.0	37.0	39.0	30.0	39.0
14-15	37.11698267679874	41.0	38.0	41.0	30.5	41.0
16-17	37.1452729141468	41.0	38.0	41.0	30.5	41.0
18-19	37.17968559859659	41.0	38.0	41.0	30.5	41.0
20-21	37.283548088641716	41.0	38.0	41.0	30.5	41.0
22-23	37.259708616145225	40.5	38.0	41.0	30.0	41.0
24-25	37.30340549761934	41.0	38.0	41.0	30.0	41.0
26-27	37.34180806686177	41.0	38.0	41.0	30.0	41.0
28-29	37.310579791024765	40.0	38.0	41.0	30.0	41.0
30-31	37.24197813355883	40.0	38.0	41.0	30.0	41.0
32-33	37.192296482405126	40.0	38.0	41.0	30.0	41.0
34-35	37.121476458656055	40.0	38.0	41.0	30.0	41.0
36-37	36.989525934193644	40.0	38.0	41.0	30.0	41.0
38-39	36.86165351389127	40.0	37.5	41.0	30.0	41.0
40-41	36.80461721996044	40.0	37.0	41.0	30.0	41.0
42-43	36.63804237658145	40.0	37.0	41.0	29.0	41.0
44-45	36.47551699769824	40.0	37.0	41.0	29.0	41.0
46-47	36.34371450234385	40.0	36.0	41.0	29.0	41.0
48-49	36.210765500269375	40.0	36.0	41.0	29.0	41.0
50-51	36.078315794510296	40.0	36.0	41.0	28.0	41.0
52-53	35.92030996168036	40.0	35.0	41.0	28.0	41.0
54-55	35.76239522368613	40.0	35.0	41.0	28.0	41.0
56-57	35.57566932850684	39.0	35.0	41.0	28.0	41.0
58-59	35.364247186296765	39.0	35.0	41.0	27.5	41.0
60-61	35.132878265554325	39.0	34.5	41.0	27.0	41.0
62-63	35.046477689356486	39.0	34.5	41.0	27.0	41.0
64-65	34.80959488562307	38.0	34.0	40.0	26.5	41.0
66-67	34.55535322482619	38.0	34.0	40.0	26.0	41.0
68-69	34.27008238561732	37.0	34.0	40.0	26.0	41.0
70-71	33.94373191617987	37.0	34.0	40.0	26.0	41.0
72-73	33.60392471517912	36.0	33.0	39.0	25.5	41.0
74-75	33.32035500301579	36.0	33.0	39.0	25.0	41.0
76-77	33.04977872696347	35.5	33.0	39.0	25.0	40.5
78-79	32.694157370469355	35.0	33.0	37.5	24.5	39.5
80-81	32.35392609540389	35.0	33.0	37.0	24.0	39.0
82-83	32.01242731391234	35.0	33.0	37.0	23.0	39.0
84-85	31.696884464603905	35.0	32.0	36.0	22.0	38.0
86-87	31.402078802553785	35.0	32.0	36.0	20.5	37.0
88-89	31.1247615086593	35.0	32.0	35.5	20.0	37.0
90-91	30.884942276699185	35.0	32.0	35.0	19.0	36.5
92-93	30.645159505677093	35.0	32.0	35.0	17.5	36.0
94-95	30.431825982179458	35.0	31.5	35.0	10.0	36.0
96-97	30.224891525833897	35.0	31.0	35.0	2.0	36.0
98-99	30.00821532794831	35.0	31.0	35.0	2.0	35.5
100	29.836209875140266	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33503297517266617
1101	2	0.3210235289917698
1101	3	0.3610900733268956
1101	4	0.42208210206198515
1101	5	0.35379857481977695
1101	6	0.3663311726570626
1101	7	0.4144851351401684
1101	8	0.3579978108499944
1101	9	0.3819360871094233
1101	10-11	0.4324240897534182
1101	12-13	0.3838114580494292
1101	14-15	0.39788871035986517
1101	16-17	0.3997030172759821
1101	18-19	0.3186670379452252
1101	20-21	0.3389329360405924
1101	22-23	0.4122204525742177
1101	24-25	0.4032179098533035
1101	26-27	0.42101910732272785
1101	28-29	0.4397749394037902
1101	30-31	0.40846848122528456
1101	32-33	0.42895981523228954
1101	34-35	0.4204196417805903
1101	36-37	0.37784469186678393
1101	38-39	0.44673956128686854
1101	40-41	0.3099467614400737
1101	42-43	0.3320405023331361
1101	44-45	0.3500228475512728
1101	46-47	0.33353402800526766
1101	48-49	0.3308827701746395
1101	50-51	0.34469319258783315
1101	52-53	0.27750486933778973
1101	54-55	0.3104414461036811
1101	56-57	0.31208453072063946
1101	58-59	0.3421159583585123
1101	60-61	0.3434731507227937
1101	62-63	0.3156655182059609
1101	64-65	0.3960080575366334
1101	66-67	0.34450508945898406
1101	68-69	0.356572373551991
1101	70-71	0.3167017981924687
1101	72-73	0.3003372261464108
1101	74-75	0.34747123299696625
1101	76-77	0.37222181545662636
1101	78-79	0.34227418404676513
1101	80-81	0.3536854406805148
1101	82-83	0.36144417986815114
1101	84-85	0.34766560248362666
1101	86-87	0.30614056717552884
1101	88-89	0.3516463155803713
1101	90-91	0.35549826533006623
1101	92-93	0.2594907394731898
1101	94-95	0.28571977192388687
1101	96-97	0.35272761532504404
1101	98-99	0.30257477749882966
1101	100	0.3010494408016804
1102	1	0.06532902085891479
1102	2	0.0637191584344663
1102	3	0.106913310575667
1102	4	0.10508523896992017
1102	5	0.1209647253712518
1102	6	0.09904009876880338
1102	7	0.04734877013278549
1102	8	0.0480148778410765
1102	9	0.1355750762609489
1102	10-11	0.1370614601800284
1102	12-13	0.0815583136008513
1102	14-15	0.1057194178733667
1102	16-17	0.05803756549085648
1102	18-19	0.05744234937382231
1102	20-21	0.05779478892765155
1102	22-23	0.03554925733186565
1102	24-25	0.06004634009229193
1102	26-27	0.03683485419838206
1102	28-29	0.00938796611777093
1102	30-31	0.07142337057890558
1102	32-33	0.08926743580718721
1102	34-35	0.07627432351513619
1102	36-37	0.04516410600901111
1102	38-39	0.09078886201780989
1102	40-41	0.06624338775591809
1102	42-43	0.015569536326360378
1102	44-45	-0.024214479139594403
1102	46-47	-0.07491344135458888
1102	48-49	-0.07619820880204031
1102	50-51	-0.14291225075176328
1102	52-53	-0.10453626990272369
1102	54-55	-0.115338319332011
1102	56-57	-0.10791231477659124
1102	58-59	-0.129094051092423
1102	60-61	-0.10905189678326366
1102	62-63	-0.017857106239794973
1102	64-65	-0.07261852725539342
1102	66-67	-0.06814446664843388
1102	68-69	-0.11148307029031201
1102	70-71	-0.07721981429885716
1102	72-73	-0.08711727863435925
1102	74-75	-0.10088559782114004
1102	76-77	-0.13790105933851748
1102	78-79	-0.14944549753697345
1102	80-81	-0.17199818154326607
1102	82-83	-0.13369047089948793
1102	84-85	-0.12813043817552838
1102	86-87	-0.16145345208830264
1102	88-89	-0.17199894487347223
1102	90-91	-0.21958669259314334
1102	92-93	-0.16165228392291553
1102	94-95	-0.1692052740740415
1102	96-97	-0.10804758802099101
1102	98-99	-0.18721418500908982
1102	100	-0.23699310853927003
1103	1	-0.31502854811376935
1103	2	-0.3215721811526926
1103	3	-0.22527280055890486
1103	4	-0.21637788721036344
1103	5	-0.20088857047964126
1103	6	-0.17013192140677091
1103	7	-0.21551864163627243
1103	8	-0.2359301923401773
1103	9	-0.2330203242699227
1103	10-11	-0.2136120779033135
1103	12-13	-0.24543143889881236
1103	14-15	-0.20704008334175938
1103	16-17	-0.23685560110687476
1103	18-19	-0.32421681462790275
1103	20-21	-0.3390421668665198
1103	22-23	-0.25927142685419824
1103	24-25	-0.2504722066441829
1103	26-27	-0.22608362300540108
1103	28-29	-0.28172281612627614
1103	30-31	-0.27697687956735706
1103	32-33	-0.2702936812993073
1103	34-35	-0.29852021405905305
1103	36-37	-0.3413793136657972
1103	38-39	-0.27067979047365753
1103	40-41	-0.290939713782862
1103	42-43	-0.3690228837277445
1103	44-45	-0.5528760920383888
1103	46-47	-0.5947463057372175
1103	48-49	-0.5086152173861009
1103	50-51	-0.5595096263338206
1103	52-53	-0.5927603613167776
1103	54-55	-0.5832382164574099
1103	56-57	-0.5604863164826384
1103	58-59	-0.5399508448814743
1103	60-61	-0.48462879060056707
1103	62-63	-0.4587447245925489
1103	64-65	-0.5049732639936053
1103	66-67	-0.4713777872794864
1103	68-69	-0.538687244672154
1103	70-71	-0.5561945323076145
1103	72-73	-0.5948857292213319
1103	74-75	-0.5936918760333469
1103	76-77	-0.6108871922556318
1103	78-79	-0.5795485902165112
1103	80-81	-0.607148646836233
1103	82-83	-0.5762551053568181
1103	84-85	-0.6193592217857784
1103	86-87	-0.6394368502125083
1103	88-89	-0.6889065577831062
1103	90-91	-0.6505232321617953
1103	92-93	-0.6334393434551799
1103	94-95	-0.6909335220077573
1103	96-97	-0.6492436158839894
1103	98-99	-0.7011497669275109
1103	100	-0.6996969747763195
1104	1	-0.28747719679451933
1104	2	-0.24451911795921433
1104	3	-0.1661317062768468
1104	4	-0.16498423918100258
1104	5	-0.16382679851570003
1104	6	-0.2626024751996425
1104	7	-0.13390835797182632
1104	8	-0.20433848637557617
1104	9	-0.27160108689711393
1104	10-11	-0.271780579343023
1104	12-13	-0.2106065944424813
1104	14-15	-0.30391945218549665
1104	16-17	-0.25779171093975606
1104	18-19	-0.25380873612513
1104	20-21	-0.2850424476006168
1104	22-23	-0.2425205735012952
1104	24-25	-0.24046295991183086
1104	26-27	-0.22431377810713826
1104	28-29	-0.15891200728182753
1104	30-31	-0.23915117688166987
1104	32-33	-0.31067784621610883
1104	34-35	-0.19232843927012055
1104	36-37	-0.2990018570963997
1104	38-39	-0.2563351424725582
1104	40-41	-0.3197538591836988
1104	42-43	-0.3850136578181136
1104	44-45	-0.35838043353987814
1104	46-47	-0.48222393714546996
1104	48-49	-0.49816680491161947
1104	50-51	-0.494959791222243
1104	52-53	-0.5403590348941094
1104	54-55	-0.4185017023604587
1104	56-57	-0.40788434642895055
1104	58-59	-0.533284764133569
1104	60-61	-0.5639843306525449
1104	62-63	-0.47949859828460717
1104	64-65	-0.4382722522500586
1104	66-67	-0.3415939239642469
1104	68-69	-0.4097410488863815
1104	70-71	-0.4961909596443519
1104	72-73	-0.43645469989798613
1104	74-75	-0.4291267394470566
1104	76-77	-0.4349178476842823
1104	78-79	-0.45220427843537436
1104	80-81	-0.4124147445255879
1104	82-83	-0.488582649748178
1104	84-85	-0.477600612712191
1104	86-87	-0.41619360201307387
1104	88-89	-0.44151013799522687
1104	90-91	-0.4995685436351387
1104	92-93	-0.5499979891151412
1104	94-95	-0.5238499038217448
1104	96-97	-0.47188579542027753
1104	98-99	-0.48144061769671254
1104	100	-0.48086685277724683
1105	1	-0.14299387226569849
1105	2	-0.16545292228510178
1105	3	-0.037612537235304444
1105	4	0.01371891193603858
1105	5	-0.012366313592103495
1105	6	-0.013423554449225605
1105	7	0.03551286644481877
1105	8	0.04472403326862917
1105	9	0.05245283321883676
1105	10-11	-0.06069595822788898
1105	12-13	0.03128415289459241
1105	14-15	-0.014394663484004866
1105	16-17	-0.0775802381929438
1105	18-19	0.02986905663268402
1105	20-21	0.009755286809237873
1105	22-23	-0.07333168764485265
1105	24-25	-0.05691025075783074
1105	26-27	-0.09819393601829773
1105	28-29	-0.1463288280762498
1105	30-31	-0.06810195976085964
1105	32-33	-0.0695788460259621
1105	34-35	-0.05757662546269415
1105	36-37	-0.09810585897131574
1105	38-39	-0.04546663209045221
1105	40-41	-0.12313726109852041
1105	42-43	-0.20107212748472136
1105	44-45	-0.18628886525532096
1105	46-47	-0.17459892284545475
1105	48-49	-0.18768532698751983
1105	50-51	-0.13847250866535887
1105	52-53	-0.1587444993291598
1105	54-55	-0.2608912979854594
1105	56-57	-0.28899406235363756
1105	58-59	-0.22235952224377087
1105	60-61	-0.3272099121895451
1105	62-63	-0.18145797054052082
1105	64-65	-0.19536714370077846
1105	66-67	-0.1929769570071258
1105	68-69	-0.22711674226605538
1105	70-71	-0.13414477998234986
1105	72-73	-0.12038904362220393
1105	74-75	-0.17774697149180696
1105	76-77	-0.2340035230957227
1105	78-79	-0.19310276124822678
1105	80-81	-0.1236516753900716
1105	82-83	-0.14239452242674489
1105	84-85	-0.1191980927087748
1105	86-87	-0.09944909790228351
1105	88-89	-0.11528445874962401
1105	90-91	-0.14419100042958988
1105	92-93	-0.1098381140259761
1105	94-95	-0.17503893258067293
1105	96-97	-0.17898266593383383
1105	98-99	-0.18923466250874554
1105	100	-0.16880795684344818
1106	1	-0.131983294120797
1106	2	-0.11077724625643981
1106	3	-0.04716409733689986
1106	4	0.030475099870386657
1106	5	0.04403592634102438
1106	6	0.038621606062925196
1106	7	-0.026345253965793347
1106	8	-0.03904519046808019
1106	9	0.002145088255915084
1106	10-11	-0.05489301718247219
1106	12-13	-0.03907474268837774
1106	14-15	-0.06584893753394283
1106	16-17	-0.056077319964792594
1106	18-19	-0.09633345925873016
1106	20-21	-0.10181248968298462
1106	22-23	-0.03594978811149474
1106	24-25	-0.053355808920336756
1106	26-27	-0.1949226164736544
1106	28-29	-0.1130304120706711
1106	30-31	-0.0363422205537276
1106	32-33	-0.14430557664242372
1106	34-35	-0.031238387185922534
1106	36-37	-0.051433943181613984
1106	38-39	-0.10211404236944333
1106	40-41	-0.08560535918356038
1106	42-43	-0.060650355743398165
1106	44-45	-0.08209530955738131
1106	46-47	-0.10120156672866898
1106	48-49	-0.06620990279384387
1106	50-51	-0.13704791818530282
1106	52-53	-0.17194230810358135
1106	54-55	-0.14309655002787736
1106	56-57	-0.16169005650257873
1106	58-59	-0.23860846780961964
1106	60-61	-0.23957078065916448
1106	62-63	-0.27526309305463315
1106	64-65	-0.16767494666717653
1106	66-67	-0.14016416266826326
1106	68-69	-0.17913710725008514
1106	70-71	-0.21520709454406273
1106	72-73	-0.20639111330935833
1106	74-75	-0.15695344352798202
1106	76-77	-0.16417102831603358
1106	78-79	-0.17857567400638175
1106	80-81	-0.2465506337089991
1106	82-83	-0.19483375129542324
1106	84-85	-0.20054816792123376
1106	86-87	-0.22912284368262448
1106	88-89	-0.20846354699635228
1106	90-91	-0.2307217756941391
1106	92-93	-0.16490915484659752
1106	94-95	-0.19320170494735223
1106	96-97	-0.2013805567582203
1106	98-99	-0.23841042727120865
1106	100	-0.32615210033561226
1107	1	-0.3324270097797708
1107	2	-0.4841147083436823
1107	3	-0.3875988542717721
1107	4	-0.2962985254641666
1107	5	-0.25285100456778054
1107	6	-0.3438879258889216
1107	7	-0.29189131833972226
1107	8	-0.28526172170348474
1107	9	-0.35216970561913996
1107	10-11	-0.31154592496363875
1107	12-13	-0.3361267575117708
1107	14-15	-0.4242474409245247
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2216	56-57	0.33569104179457554
2216	58-59	0.3917828417977418
2216	60-61	0.37368296479802154
2216	62-63	0.3191629902672872
2216	64-65	0.2719498698196645
2216	66-67	0.2311861082416371
2216	68-69	0.24733089214930715
2216	70-71	0.22819336259728829
2216	72-73	0.22275662543662378
2216	74-75	0.2528045026684751
2216	76-77	0.2897942328010146
2216	78-79	0.3068596528718075
2216	80-81	0.3024156088122112
2216	82-83	0.30435535469481323
2216	84-85	0.27103835779979235
2216	86-87	0.27178189562865995
2216	88-89	0.29933496814151184
2216	90-91	0.35471216262661187
2216	92-93	0.388524300538581
2216	94-95	0.2956808334486105
2216	96-97	0.29503255225954206
2216	98-99	0.2599076531116751
2216	100	0.26114121053603867
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92868.0
3	18320.0
4	12378.0
5	10651.0
6	7649.0
7	6859.0
8	7836.0
9	8265.0
10	8620.0
11	9288.0
12	10480.0
13	11714.0
14	12033.0
15	12716.0
16	12666.0
17	12731.0
18	12461.0
19	12320.0
20	12415.0
21	12547.0
22	14924.0
23	21383.0
24	33760.0
25	46382.0
26	53079.0
27	43230.0
28	39338.0
29	48256.0
30	58503.0
31	66211.0
32	83428.0
33	111306.0
34	157048.0
35	242249.0
36	410997.0
37	764539.0
38	1213186.0
39	632062.0
40	2420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53124273393308	21.770352073420085	14.518491787457648	22.17991340518919
2	14.663245291922902	22.624163090445222	40.383351583123186	22.329240034508693
3	16.867711723006295	28.562256289235705	31.37664893666055	23.193383051097445
4	11.683924737971967	19.3528591957069	40.21082990829595	28.75238615802519
5	11.842210793076015	40.15038935202641	35.37023704057429	12.637162814323288
6	27.1496273118338	40.763576793468275	19.19283050089713	12.893965393800794
7	24.33208380460204	35.34442768737118	23.815159684983396	16.50832882304339
8	23.47898585936617	37.428103885353856	21.559500300279286	17.533409955000693
9	24.381505718783313	18.579652681536356	22.14490684060441	34.893934759075925
10-11	21.73687042648273	29.27940791385745	30.02179461724175	18.96192704241807
12-13	22.801992897832562	27.402978251050985	29.32289079301091	20.472138058105546
14-15	20.764348306296398	29.073432380650715	26.72195444001419	23.4402648730387
16-17	19.937831274105545	32.013981978481496	27.434850648774432	20.61333609863853
18-19	19.97280212886934	30.941532058497867	29.240922724897505	19.844743087735292
20-21	21.78449220185614	29.943707600423526	28.42223477845205	19.849565419268284
22-23	21.303864763046317	29.976058378836534	28.47453050493559	20.245546353181556
24-25	20.968830356566336	30.001361612360817	28.447224399817273	20.582583631255577
26-27	20.614534875155158	29.999917126156088	28.484212072324926	20.901335926363824
28-29	20.59598624328393	29.924195338001773	28.72225521746937	20.75756320124492
30-31	20.959486056702783	30.297752522517307	28.314028728824397	20.428732691955513
32-33	20.537394773820115	30.181692992778963	28.737417057552804	20.54349517584812
34-35	20.82519325269853	29.860737430589424	28.63967066211156	20.67439865460049
36-37	21.049458663116845	29.972158436065243	28.251681936205454	20.72670096461246
38-39	20.79859106697324	30.021623208257914	28.543601815267472	20.636183909501376
40-41	20.675985272308967	29.768405354971517	28.731509238417047	20.824100134302473
42-43	20.623611806870336	29.808857546982164	28.67655928850843	20.890971357639067
44-45	20.844052864653246	29.916632859947168	28.585772444016307	20.653541831383286
46-47	20.875497381523974	30.07873615136053	28.418246178185292	20.627520288930203
48-49	20.799246141031304	29.787271515854197	28.512083983535923	20.90139835957857
50-51	20.77296415427052	29.753061678118808	28.45908244795196	21.014891719658706
52-53	20.749757723634694	29.78761292369299	28.556138763378154	20.90649058929416
54-55	20.955480183326035	30.056790090770907	28.367499284871915	20.620230441031143
56-57	20.868791520077256	29.85164854454198	28.199737218161296	21.07982271721946
58-59	20.72072839488551	29.60634067121622	28.562236018324068	21.11069491557421
60-61	20.963631834656923	29.905790949006207	28.23101453492655	20.899562681410323
62-63	20.78749189560865	29.87428568271577	28.344738277507943	20.99348414416764
64-65	20.80357747144197	29.680899602712913	28.453621371457672	21.061901554387447
66-67	20.657619869941506	29.69866148891273	28.345164105126454	21.29855453601931
68-69	21.106909943071294	29.874298750238832	28.136534508279176	20.882256798410694
70-71	20.991299011398826	29.627822630488932	28.237100845974844	21.143777512137394
72-73	20.79208068931426	29.729744660697566	28.540950333395294	20.937224316592882
74-75	20.793130710849926	29.816266404333152	28.38861362976376	21.00198925505316
76-77	20.87518353466705	29.8004243224814	28.222981556060887	21.10141058679066
78-79	20.9518520351611	29.766920157780664	28.44151882882271	20.83970897823552
80-81	20.82015662977209	29.704093243463355	28.439532585542338	21.036217541222225
82-83	20.718620402432123	29.65992953564364	28.695490817612846	20.92595924431139
84-85	20.732578106321085	29.660159355808112	28.41753537802772	21.189727159843084
86-87	20.927084731426778	29.6758326344305	28.329357855825187	21.067724778317537
88-89	20.816495908656886	29.54377365896783	28.47986904939368	21.1598613829816
90-91	20.694819621618816	29.550933498108805	28.40948424363303	21.344762636639345
92-93	20.8312107211234	29.630243063172852	28.357700115812328	21.180846099891415
94-95	20.677481322389767	29.518830691247107	28.50171669423276	21.30197129213037
96-97	20.671629559683858	29.641081502154876	28.41873563858709	21.26855329957418
98-99	20.666550406536352	29.59083916216734	28.387224508095905	21.355385923200398
100	20.718288713023654	29.785343160154454	28.242271923222418	21.254096203599477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14724.0
1	10861.5
2	6146.5
3	4502.5
4	3176.0
5	2394.0
6	2033.0
7	1896.0
8	1936.5
9	1909.5
10	1702.5
11	1518.5
12	1454.0
13	1479.0
14	1602.5
15	1791.0
16	2085.5
17	2652.5
18	3493.0
19	4653.0
20	6625.5
21	9369.5
22	13838.0
23	21375.0
24	29216.0
25	36889.5
26	47631.0
27	61554.0
28	79340.5
29	94895.5
30	104116.5
31	114587.5
32	125811.5
33	135787.5
34	144669.0
35	150751.5
36	152876.5
37	152996.5
38	152692.0
39	151518.0
40	151361.5
41	148146.0
42	141129.5
43	135975.5
44	133201.5
45	130032.0
46	126716.5
47	123278.5
48	119127.5
49	116451.0
50	115084.5
51	110078.5
52	102415.0
53	95677.5
54	91192.0
55	88883.0
56	82145.5
57	73328.0
58	66907.5
59	60668.0
60	53433.5
61	45423.5
62	38580.5
63	31581.5
64	24845.0
65	19877.5
66	15820.0
67	12195.0
68	9381.5
69	7336.0
70	5594.0
71	4415.5
72	3511.5
73	2664.5
74	2179.5
75	1766.0
76	1262.5
77	978.0
78	760.0
79	560.0
80	415.5
81	290.0
82	203.0
83	152.5
84	127.5
85	112.5
86	93.0
87	85.0
88	72.0
89	57.0
90	51.0
91	47.0
92	43.5
93	32.5
94	24.5
95	23.5
96	18.0
97	16.5
98	19.5
99	23.5
100	34.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17475946132587153
2	0.16889350599638658
3	0.18306381377271103
4	0.17266612040436904
5	0.1866523982095724
6	0.07526825818852859
7	0.07699353916778887
8	0.06507759853769784
9	0.05923464695460303
10-11	0.07258832173407762
12-13	0.0711275838383039
14-15	0.06898823542402116
16-17	0.06467503297587045
18-19	0.06965534406933513
20-21	0.07139212692179049
22-23	0.0692297747611176
24-25	0.06909175228277677
26-27	0.07282986107117406
28-29	0.07034545646103925
30-31	0.0757398349895264
32-33	0.07227777115781076
34-35	0.07199022432793405
36-37	0.0830895319611752
38-39	0.07833925833161189
40-41	0.07342795847731762
42-43	0.07687852043583818
44-45	0.08741423628252097
46-47	0.07752262533476202
48-49	0.08281348700449355
50-51	0.0796504718758497
52-53	0.07870731827385408
54-55	0.08029457677477354
56-57	0.07385352778553515
58-59	0.08143326222108532
60-61	0.07761464032032257
62-63	0.06952882346418937
64-65	0.07259982360727268
66-67	0.07245029925573679
68-69	0.07103556885274336
70-71	0.06668786078500745
72-73	0.06442199176557893
74-75	0.06968984968892034
76-77	0.07480818326072584
78-79	0.07161066251249679
80-81	0.079029370723316
82-83	0.08059362547784532
84-85	0.0820658652468141
86-87	0.07832775645841682
88-89	0.08040959550672422
90-91	0.07889134824497518
92-93	0.06949431784460418
94-95	0.06931028787348308
96-97	0.06928728412709294
98-99	0.07845427706356256
100	0.08665511265164644
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4347118.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.36979546904637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43248572352562	48.43510987490625
2	14.610518085155746	18.517310855062455
3	4.501067367173735	8.556951553505957
4	1.7769696278352014	4.504248074824966
5	0.8460391741100073	2.6806664711076036
6	0.47743109424502894	1.8152826475722292
7	0.285381489356648	1.2659196627827831
8	0.19941342953820185	1.0109430594893551
9	0.13794226650778008	0.7867235893621238
>10	0.6756137287278199	7.467281204067325
>50	0.03791955351706386	1.640052305355616
>100	0.017547290908829737	2.0946431239443486
>500	0.0011988825884231876	0.48970314001332527
>1k	4.3595705532702156E-4	0.37454640158841934
>5k	0.0	0.0
>10k+	3.6329754610526515E-5	0.3606180364173449
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15664	0.3603306834551075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3003746390137098E-5	0.0
4	0.0	0.0	0.0	6.90112391704113E-5	0.0
5	0.0	0.0	0.0	6.90112391704113E-5	0.0
6	0.0	0.0	0.0	6.90112391704113E-5	0.0
7	0.0	0.0	0.0	9.201498556054839E-5	0.0
8	0.0	0.0	0.0	9.201498556054839E-5	0.0
9	0.0	0.0	0.0	1.380224783408226E-4	0.0
10-11	0.0	0.0	0.0	1.9553184431616533E-4	0.0
12-13	0.0	0.0	0.0	2.530412102915081E-4	0.0
14-15	0.0	0.0	0.0	2.530412102915081E-4	0.0
16-17	0.0	0.0	0.0	3.2205244946191937E-4	0.0
18-19	0.0	0.0	0.0	3.56558069047125E-4	0.0
20-21	0.0	0.0	0.0	4.025655618273992E-4	1.1501873195068549E-5
22-23	0.0	0.0	0.0	5.635917865583588E-4	2.3003746390137098E-5
24-25	0.0	0.0	0.0	7.706255040695927E-4	2.3003746390137098E-5
26-27	0.0	0.0	0.0	0.0011271835731167176	2.3003746390137098E-5
28-29	0.0	0.0	0.0	0.0022083596534531614	2.3003746390137098E-5
30-31	0.0	0.0	0.0	0.004738771756368242	2.3003746390137098E-5
32-33	0.0	0.0	0.0	0.009811097835393471	2.3003746390137098E-5
34-35	0.0	0.0	0.0	0.018472008351280086	2.3003746390137098E-5
36-37	0.0	1.1501873195068549E-5	0.0	0.032251252438972214	2.3003746390137098E-5
38-39	0.0	2.3003746390137098E-5	0.0	0.05170092001183313	2.3003746390137098E-5
40-41	0.0	2.3003746390137098E-5	0.0	0.0731749172670261	2.3003746390137098E-5
42-43	0.0	2.3003746390137098E-5	0.0	0.09461440890263388	3.450561958520565E-5
44-45	0.0	2.3003746390137098E-5	0.0	0.1216323090378499	4.6007492780274196E-5
46-47	0.0	2.3003746390137098E-5	0.0	0.15549382372413173	4.6007492780274196E-5
48-49	0.0	2.3003746390137098E-5	0.0	0.1920352748648645	4.6007492780274196E-5
50-51	0.0	2.3003746390137098E-5	0.0	0.2298074264374696	4.6007492780274196E-5
52-53	0.0	2.3003746390137098E-5	0.0	0.2681431697966331	6.90112391704113E-5
54-55	0.0	2.3003746390137098E-5	0.0	0.312758935920304	6.90112391704113E-5
56-57	0.0	2.3003746390137098E-5	0.0	0.3661506312918122	6.90112391704113E-5
58-59	1.1501873195068549E-5	2.3003746390137098E-5	0.0	0.4286518102338147	6.90112391704113E-5
60-61	2.3003746390137098E-5	2.3003746390137098E-5	0.0	0.49682341266098595	6.90112391704113E-5
62-63	2.3003746390137098E-5	2.3003746390137098E-5	0.0	0.5657426368458367	8.051311236547984E-5
64-65	2.3003746390137098E-5	4.6007492780274196E-5	0.0	0.639803198348883	9.201498556054839E-5
66-67	2.3003746390137098E-5	4.6007492780274196E-5	0.0	0.7307715134486803	9.201498556054839E-5
68-69	2.3003746390137098E-5	4.6007492780274196E-5	0.0	0.8358871325784117	9.201498556054839E-5
70-71	2.3003746390137098E-5	4.6007492780274196E-5	0.0	0.9554261006947591	9.201498556054839E-5
72-73	2.3003746390137098E-5	4.6007492780274196E-5	0.0	1.077886544602654	9.201498556054839E-5
74-75	2.3003746390137098E-5	5.7509365975342745E-5	0.0	1.2039700785669956	9.201498556054839E-5
76-77	2.3003746390137098E-5	6.90112391704113E-5	0.0	1.3461102275116525	9.201498556054839E-5
78-79	2.3003746390137098E-5	6.90112391704113E-5	0.0	1.5112656247196419	9.201498556054839E-5
80-81	2.3003746390137098E-5	6.90112391704113E-5	0.0	1.6987116521796741	9.201498556054839E-5
82-83	2.3003746390137098E-5	6.90112391704113E-5	0.0	1.8977630696935304	9.201498556054839E-5
84-85	2.3003746390137098E-5	6.90112391704113E-5	0.0	2.0976771276970165	9.201498556054839E-5
86-87	2.3003746390137098E-5	6.90112391704113E-5	0.0	2.314993519844642	9.201498556054839E-5
88	2.3003746390137098E-5	6.90112391704113E-5	0.0	2.491535771515749	1.1501873195068549E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5125	0.0	48.65998	1
GTATCAA	9175	0.0	38.104183	1
TCAACGC	10285	0.0	32.796284	4
ATCAACG	10405	0.0	32.42141	3
CAACGCA	11075	0.0	30.584303	5
TATCAAC	11580	0.0	29.700834	2
AACGCAG	12030	0.0	28.39902	6
CGCAGAG	13160	0.0	25.495697	8
GTACATG	19190	0.0	25.230759	1
ACGCAGA	13505	0.0	25.123327	7
TACATGG	19080	0.0	24.634684	2
ACATGGG	19480	0.0	23.403978	3
GTGGTAT	2640	0.0	23.170176	1
CATGGGT	8385	0.0	22.947218	4
TGGTATC	2555	0.0	22.09786	2
GCAGAGT	14975	0.0	21.527412	9
ATGGGTG	5020	0.0	19.492584	5
TATACCG	275	3.1746822E-8	18.817842	5
CAGAGTA	14710	0.0	18.337337	10-11
AGAGTAC	13365	0.0	18.248848	10-11
>>END_MODULE