FastQCFastQC Report
Wed 25 May 2016
SRR2431426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347118
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282110.6489586894121576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231430.5323757027069429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139080.3199361047940268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.11727309909691892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.065.2624741
GTATCAA227000.045.5138551
GTGGTAT35750.037.0947571
ATCAACG291350.035.16633
TGGTATC37650.034.7207642
TCAACGC295600.034.6602974
TATCAAC298850.034.3801422
CAACGCA303200.033.7915045
AACGCAG316100.032.4871446
CGCAGAG353000.028.8515288
ACGCAGA355350.028.753317
GCAGAGT381150.026.5357259
GTACATG190900.024.6831871
GAGTACT267800.023.81518212-13
TACATGG189600.023.8090672
CATGGGT82650.023.6093864
TATAACG3850.023.206112
CAGAGTA374950.022.78794310-11
ACATGGG191750.022.7560623
AGAGTAC361900.022.29802110-11
Produced by FastQC (version 0.11.5)