##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4347118 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66118817110554 34.0 31.0 34.0 31.0 34.0 2 32.77491386247164 34.0 31.0 34.0 31.0 34.0 3 32.84996358506947 34.0 31.0 34.0 31.0 34.0 4 36.31640640994792 37.0 37.0 37.0 35.0 37.0 5 36.22283016011988 37.0 37.0 37.0 35.0 37.0 6 36.270093197378124 37.0 37.0 37.0 35.0 37.0 7 36.22886657321011 37.0 37.0 37.0 35.0 37.0 8 36.24370629000639 37.0 37.0 37.0 35.0 37.0 9 38.03786255629592 39.0 39.0 39.0 35.0 39.0 10-11 38.01906216946492 39.0 39.0 39.0 35.0 39.0 12-13 38.003321625960005 39.0 39.0 39.0 35.0 39.0 14-15 39.47400611163534 41.0 39.0 41.0 36.0 41.0 16-17 39.50900999236735 41.0 39.0 41.0 36.5 41.0 18-19 39.58648453987216 41.0 40.0 41.0 37.0 41.0 20-21 39.584842532454836 41.0 40.0 41.0 37.0 41.0 22-23 39.53047283280555 41.0 40.0 41.0 36.0 41.0 24-25 39.4877413035487 41.0 39.0 41.0 36.0 41.0 26-27 39.375575611244045 41.0 39.0 41.0 36.0 41.0 28-29 39.294017898754994 41.0 39.0 41.0 36.0 41.0 30-31 39.12083384900065 41.0 39.0 41.0 35.5 41.0 32-33 38.90170188616918 41.0 38.0 41.0 35.0 41.0 34-35 38.76975527234365 41.0 38.0 41.0 35.0 41.0 36-37 38.62629424828127 41.0 38.0 41.0 35.0 41.0 38-39 38.47357996723346 41.0 38.0 41.0 35.0 41.0 40-41 38.33089370934951 41.0 38.0 41.0 35.0 41.0 42-43 38.15999278142438 40.0 38.0 41.0 34.0 41.0 44-45 37.92101629171327 40.0 38.0 41.0 33.5 41.0 46-47 37.56574436212682 40.0 38.0 41.0 33.0 41.0 48-49 37.24161720477797 40.0 38.0 41.0 32.0 41.0 50-51 37.07696719987817 40.0 37.0 41.0 32.0 41.0 52-53 37.017438219988506 40.0 37.0 41.0 32.0 41.0 54-55 36.87928197486242 40.0 37.0 41.0 31.0 41.0 56-57 36.734735634045364 40.0 36.5 41.0 31.0 41.0 58-59 36.564101779615825 40.0 36.0 41.0 31.0 41.0 60-61 36.418410312303465 40.0 36.0 41.0 31.0 41.0 62-63 36.26093034511601 39.0 35.0 41.0 31.0 41.0 64-65 36.0337489113477 39.0 35.0 41.0 31.0 41.0 66-67 35.7734312710168 39.0 35.0 41.0 30.0 41.0 68-69 35.483385429150985 38.0 35.0 41.0 30.0 41.0 70-71 35.184121871087925 37.0 35.0 40.0 29.5 41.0 72-73 34.86581558172564 37.0 35.0 40.0 29.0 41.0 74-75 34.53003392132443 36.5 35.0 39.0 29.0 41.0 76-77 34.19331416814542 36.0 35.0 39.0 29.0 41.0 78-79 33.86775238215296 35.5 35.0 38.5 29.0 40.0 80-81 33.59545370058967 35.0 34.5 37.0 29.0 39.0 82-83 33.38534656754199 35.0 35.0 37.0 29.0 39.0 84-85 33.112392394225324 35.0 34.0 36.5 29.0 39.0 86-87 32.863446885959846 35.0 34.0 36.0 29.0 37.0 88-89 32.626264803485896 35.0 34.0 36.0 29.0 37.0 90-91 32.43932462840898 35.0 34.0 35.5 29.0 37.0 92-93 32.27515436664015 35.0 34.0 35.0 28.5 36.0 94-95 32.133353292917285 35.0 34.0 35.0 27.5 36.0 96-97 31.9916673529451 35.0 34.0 35.0 27.0 36.0 98-99 31.876533602262462 35.0 34.0 35.0 27.0 36.0 100 31.7747567468838 35.0 34.0 35.0 26.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029554970521083135 1101 2 0.03551630251030957 1101 3 0.05669687400354917 1101 4 0.06375504779228436 1101 5 0.05257549584408849 1101 6 0.05712782314172671 1101 7 0.05470436794956868 1101 8 0.05706344093302107 1101 9 0.06604871552343639 1101 10-11 0.06286189524895036 1101 12-13 0.04729633108478737 1101 14-15 0.047151189040519625 1101 16-17 0.015624662472163209 1101 18-19 0.020032761414022104 1101 20-21 0.08313354882199064 1101 22-23 0.10632689271036355 1101 24-25 0.0732656783834642 1101 26-27 0.1301409037760095 1101 28-29 0.08647428427606485 1101 30-31 0.07905835601518163 1101 32-33 0.11568322508657758 1101 34-35 0.13391901642229698 1101 36-37 0.09353454519621351 1101 38-39 0.08532537922857841 1101 40-41 0.017954572262951274 1101 42-43 -0.041262744623978165 1101 44-45 -0.04128846330380043 1101 46-47 -0.024975182809413354 1101 48-49 0.04756959956909412 1101 50-51 -0.012381497498076044 1101 52-53 0.05841207510270863 1101 54-55 0.05646642815231928 1101 56-57 0.09014005259587776 1101 58-59 0.10793644472500574 1101 60-61 0.10173442120343879 1101 62-63 0.11026975079169432 1101 64-65 0.1293084979673509 1101 66-67 0.11718850658509439 1101 68-69 0.09104729248707599 1101 70-71 0.15159770594497957 1101 72-73 0.1309633786685609 1101 74-75 0.11131810325169766 1101 76-77 0.0482791495370094 1101 78-79 0.09155204539700179 1101 80-81 0.10944888103680483 1101 82-83 0.08649366647393464 1101 84-85 0.14195563113559473 1101 86-87 0.12483360876645833 1101 88-89 0.16809883975727757 1101 90-91 0.1637633380828163 1101 92-93 0.17009587267002502 1101 94-95 0.1729880098791341 1101 96-97 0.1644083505670828 1101 98-99 0.15730086499426932 1101 100 0.19512597495377904 1102 1 -0.031888208212286884 1102 2 -0.023219659323977737 1102 3 -0.0034132445353733942 1102 4 0.03985246285949273 1102 5 0.016646589036390935 1102 6 0.0023372519630768807 1102 7 -0.04250031529311116 1102 8 0.02314826312463225 1102 9 0.03770858723194692 1102 10-11 0.045152189937319065 1102 12-13 0.023679662239722177 1102 14-15 0.008872240507926676 1102 16-17 0.017712478299472423 1102 18-19 -0.011786494374675272 1102 20-21 0.025674909254725264 1102 22-23 0.05660941550824106 1102 24-25 0.05358905890447829 1102 26-27 0.09808462040865606 1102 28-29 0.04583247492365672 1102 30-31 0.04736936485281973 1102 32-33 0.05007754798701569 1102 34-35 0.07491366507854735 1102 36-37 0.0999579171711602 1102 38-39 0.08921126163474469 1102 40-41 0.07453328150577931 1102 42-43 0.11368932201521176 1102 44-45 0.0954665839006239 1102 46-47 0.04007657114440377 1102 48-49 -0.024117921167672307 1102 50-51 -0.027718476313886242 1102 52-53 0.010089240021621038 1102 54-55 0.04625356833791727 1102 56-57 0.04184090770491622 1102 58-59 0.04833607372531645 1102 60-61 0.04023607009094121 1102 62-63 0.044752739098662175 1102 64-65 0.033101441935336595 1102 66-67 0.07328009022089077 1102 68-69 0.08537075846872 1102 70-71 0.06855051910793009 1102 72-73 0.04291240894172432 1102 74-75 0.014087216270866065 1102 76-77 0.02546738160786788 1102 78-79 0.06497705620525807 1102 80-81 -0.002226109663062914 1102 82-83 0.0639485231514243 1102 84-85 0.06970099112593431 1102 86-87 0.02619324973125714 1102 88-89 -0.03290771054887642 1102 90-91 -0.055629061953382575 1102 92-93 0.004466379702932954 1102 94-95 -0.026308762222491566 1102 96-97 -0.005222962208776494 1102 98-99 0.012118582491577712 1102 100 0.012461287287255374 1103 1 -0.059306552363565856 1103 2 -0.0482171665592972 1103 3 -0.025800956358914107 1103 4 -0.010956068988456025 1103 5 -0.022144560646850664 1103 6 -0.0052192726578113025 1103 7 -0.047597908763592045 1103 8 -0.006148270489113372 1103 9 0.04604794711014648 1103 10-11 0.03199905250146884 1103 12-13 0.0205566283251315 1103 14-15 -0.016583796766440173 1103 16-17 0.014591259596592465 1103 18-19 -0.02312700426511327 1103 20-21 -0.07854453773850878 1103 22-23 -0.01791026876089319 1103 24-25 -0.04604187326093978 1103 26-27 -0.07384952169392989 1103 28-29 -0.02654881432793843 1103 30-31 -0.033651764841664544 1103 32-33 -0.009790617220950537 1103 34-35 -0.024445275261363975 1103 36-37 0.03642149904521119 1103 38-39 -0.05074653973545651 1103 40-41 -0.13065932064935737 1103 42-43 -0.1545429101191047 1103 44-45 -0.14061817337594817 1103 46-47 -0.09468465729508324 1103 48-49 -0.1894574495832373 1103 50-51 -0.18852737498587402 1103 52-53 -0.19640876406487706 1103 54-55 -0.11657917792989991 1103 56-57 -0.11156646525642344 1103 58-59 -0.1688799486945669 1103 60-61 -0.19864971010784416 1103 62-63 -0.19938054080357404 1103 64-65 -0.20826550575718983 1103 66-67 -0.1598638098043068 1103 68-69 -0.1287841111076915 1103 70-71 -0.14950598414640126 1103 72-73 -0.15648519288628648 1103 74-75 -0.11172458989123868 1103 76-77 -0.1395507282802555 1103 78-79 -0.1705460443175113 1103 80-81 -0.1158638455948946 1103 82-83 -0.1884151535044154 1103 84-85 -0.14789052800330182 1103 86-87 -0.15763507964747703 1103 88-89 -0.17082443241772438 1103 90-91 -0.16771692223350954 1103 92-93 -0.23924306087277358 1103 94-95 -0.24097098346931745 1103 96-97 -0.1957265687444938 1103 98-99 -0.18987594439303024 1103 100 -0.13921212990801735 1104 1 -0.11043825147057618 1104 2 -0.08007616379288152 1104 3 -0.07491205572515014 1104 4 -0.05234782533013771 1104 5 -0.11077158178392921 1104 6 -0.12835678463554245 1104 7 -0.10358883243966943 1104 8 -0.1574520751568187 1104 9 -0.09505431119420393 1104 10-11 -0.11960352600822688 1104 12-13 -0.09746218254950634 1104 14-15 -0.1616215009369455 1104 16-17 -0.15835728612595545 1104 18-19 -0.0979772688491849 1104 20-21 -0.061929591199373135 1104 22-23 -0.0933687905703593 1104 24-25 -0.10653448185102832 1104 26-27 -0.16672010543376103 1104 28-29 -0.120021455686242 1104 30-31 -0.11954000091235883 1104 32-33 -0.17316488262033403 1104 34-35 -0.14844583712714154 1104 36-37 -0.11126679177584009 1104 38-39 -0.1755822718942497 1104 40-41 -0.2010397556142749 1104 42-43 -0.1387004047841458 1104 44-45 -0.1862109150600446 1104 46-47 -0.1436039788409076 1104 48-49 -0.16017558794589348 1104 50-51 -0.20932778921403639 1104 52-53 -0.23458068808517396 1104 54-55 -0.13849253962835917 1104 56-57 -0.21913260220333086 1104 58-59 -0.17264379341694536 1104 60-61 -0.175467068967464 1104 62-63 -0.24464001944968317 1104 64-65 -0.22459618975870654 1104 66-67 -0.24263906048646788 1104 68-69 -0.2928157833215721 1104 70-71 -0.24311407262818818 1104 72-73 -0.21837861446226725 1104 74-75 -0.2393467782421581 1104 76-77 -0.2705421731457349 1104 78-79 -0.2430407191782038 1104 80-81 -0.2451645371405462 1104 82-83 -0.25255908208772126 1104 84-85 -0.2508736893988015 1104 86-87 -0.2587295599920765 1104 88-89 -0.19062965443483648 1104 90-91 -0.22162864186854847 1104 92-93 -0.30721915409518274 1104 94-95 -0.3343556376208312 1104 96-97 -0.29838137460647474 1104 98-99 -0.31820372007072706 1104 100 -0.273741587321652 1105 1 -0.13271222541312966 1105 2 -0.11111514103273379 1105 3 -0.09741941754605676 1105 4 -0.032973068625537394 1105 5 -0.10222776499117003 1105 6 -0.030166204447525047 1105 7 -0.039318486307195144 1105 8 -0.01585039788764675 1105 9 -0.04866134476887396 1105 10-11 -0.05960488084877369 1105 12-13 -0.04319914699216554 1105 14-15 -0.05252939588956451 1105 16-17 -0.04242838528817572 1105 18-19 -0.10767289533018953 1105 20-21 -0.04677664405025439 1105 22-23 -0.12575686151168242 1105 24-25 -0.1449046789510433 1105 26-27 -0.07057447549733098 1105 28-29 -0.08942871929096441 1105 30-31 -0.09874219797958261 1105 32-33 -0.06830478747694713 1105 34-35 -0.011130148639729498 1105 36-37 0.026145067939985722 1105 38-39 0.04221243374419004 1105 40-41 -0.010141721811976367 1105 42-43 -0.050415692949364654 1105 44-45 0.01957618677984385 1105 46-47 0.06638344593518042 1105 48-49 0.07246799440478924 1105 50-51 0.007088222190056115 1105 52-53 -0.016859398112984536 1105 54-55 0.052004679044074464 1105 56-57 0.06597321494317754 1105 58-59 0.10878243102507668 1105 60-61 0.11413893207814851 1105 62-63 0.09260603823763347 1105 64-65 0.07750268812486638 1105 66-67 0.030475502702394408 1105 68-69 0.10144593985599926 1105 70-71 0.09212810463181142 1105 72-73 0.1556015459086879 1105 74-75 0.16163337519841292 1105 76-77 0.06321747618873275 1105 78-79 0.11204018422713347 1105 80-81 0.17378448190805074 1105 82-83 0.061094545605222095 1105 84-85 0.05379430975549582 1105 86-87 0.07007888166017295 1105 88-89 0.13318474051171592 1105 90-91 0.12560694899224956 1105 92-93 0.07997564318045391 1105 94-95 0.10012033728845893 1105 96-97 0.10882914194307247 1105 98-99 0.07801194331113237 1105 100 0.06924644908490762 1106 1 -0.14457326520428637 1106 2 -0.12889666534886857 1106 3 -0.11929431671218538 1106 4 -0.02783284616873516 1106 5 -0.07295953461209592 1106 6 -0.06635989689539201 1106 7 -0.055250045271769466 1106 8 -0.025134220063570467 1106 9 -0.08735245202844766 1106 10-11 -0.049796722563037576 1106 12-13 -0.10576459297363527 1106 14-15 -0.06208695800707176 1106 16-17 -0.079788533515881 1106 18-19 -0.07085768213089949 1106 20-21 -0.06398125459720916 1106 22-23 -0.07339829973681589 1106 24-25 -0.011647057447191855 1106 26-27 -0.05946402380769911 1106 28-29 -0.044834941489625635 1106 30-31 -0.08075718131606635 1106 32-33 -0.07378448395356685 1106 34-35 -0.1098767068437283 1106 36-37 -0.12995492967841216 1106 38-39 -0.15097406819258907 1106 40-41 -0.17737833990938157 1106 42-43 -0.20132313053224493 1106 44-45 -0.14287293533169532 1106 46-47 -0.21234201014120657 1106 48-49 -0.1443161293124149 1106 50-51 -0.1392301700609906 1106 52-53 -0.17232380625468124 1106 54-55 -0.12940620379195877 1106 56-57 -0.13502066127201573 1106 58-59 -0.16481336614251063 1106 60-61 -0.1703744840119441 1106 62-63 -0.22805502283427614 1106 64-65 -0.2615064875649935 1106 66-67 -0.17109307854012457 1106 68-69 -0.19509743676098878 1106 70-71 -0.20569427756734626 1106 72-73 -0.17111113173178438 1106 74-75 -0.1749741298375156 1106 76-77 -0.16037634271440737 1106 78-79 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1107 34-35 -0.2946431757976882 1107 36-37 -0.35578573849117845 1107 38-39 -0.26249628430480243 1107 40-41 -0.34714834995824617 1107 42-43 -0.26846804373803934 1107 44-45 -0.14320981013847245 1107 46-47 -0.23195506981659975 1107 48-49 -0.28758855051381715 1107 50-51 -0.22626667768972197 1107 52-53 -0.30210262834948765 1107 54-55 -0.21965471493756183 1107 56-57 -0.24702637121659876 1107 58-59 -0.25091345194450554 1107 60-61 -0.2989429864552733 1107 62-63 -0.24878384205702275 1107 64-65 -0.18041613551562108 1107 66-67 -0.17083443997661618 1107 68-69 -0.19201120977491826 1107 70-71 -0.223964451280672 1107 72-73 -0.20636641278466072 1107 74-75 -0.20241514321143939 1107 76-77 -0.18587465104194933 1107 78-79 -0.18784402046234305 1107 80-81 -0.23508273196864593 1107 82-83 -0.262371083345748 1107 84-85 -0.22404648747964728 1107 86-87 -0.20739343786738118 1107 88-89 -0.19670398175801296 1107 90-91 -0.23871336152389233 1107 92-93 -0.2589454867431584 1107 94-95 -0.2740617369951899 1107 96-97 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60-61 0.1886271221555802 2214 62-63 0.17016644196753106 2214 64-65 0.1867840529299869 2214 66-67 0.19650839835476575 2214 68-69 0.18936221187018987 2214 70-71 0.14297123099034792 2214 72-73 0.16503230794427992 2214 74-75 0.18531068080280022 2214 76-77 0.1454805305512039 2214 78-79 0.17630551696002073 2214 80-81 0.20200980976863292 2214 82-83 0.18490649214408705 2214 84-85 0.16212446523088175 2214 86-87 0.11491217110579299 2214 88-89 0.13124419387466446 2214 90-91 0.1313555339715009 2214 92-93 0.14838425826280144 2214 94-95 0.13486671811048723 2214 96-97 0.14823092671570848 2214 98-99 0.18561157052670296 2214 100 0.22550035559683224 2215 1 0.08236071944541123 2215 2 0.07823846698564552 2215 3 0.06267607096651062 2215 4 0.035761899126818264 2215 5 0.009372900899045078 2215 6 0.026590119146703728 2215 7 0.010217210245606623 2215 8 0.003911398109266884 2215 9 0.02091654873267146 2215 10-11 0.0024973152992302516 2215 12-13 -0.0120798818079777 2215 14-15 0.059018485870041104 2215 16-17 0.02952311221822157 2215 18-19 0.027728685761168492 2215 20-21 0.04367063023343576 2215 22-23 0.005258714185870872 2215 24-25 0.044751214871439515 2215 26-27 0.06914295434561524 2215 28-29 0.08769867089588956 2215 30-31 0.03211656362410764 2215 32-33 0.02320956307908517 2215 34-35 0.029137230927531732 2215 36-37 -0.05333492130587558 2215 38-39 0.002580678893259858 2215 40-41 -0.0176024463987261 2215 42-43 -0.0241623308453498 2215 44-45 0.004306507969900508 2215 46-47 0.041586144380538315 2215 48-49 0.14307739872987213 2215 50-51 0.201018656336295 2215 52-53 0.12743633462386583 2215 54-55 0.07391266733102242 2215 56-57 0.0884195459835766 2215 58-59 0.10362148239651248 2215 60-61 0.08432150794536142 2215 62-63 0.11256712586269657 2215 64-65 0.08353590297233637 2215 66-67 0.09212495725065395 2215 68-69 0.1208723048554674 2215 70-71 0.06231875143564025 2215 72-73 0.07626782828801026 2215 74-75 0.07696372242877203 2215 76-77 0.05645917947099832 2215 78-79 0.07898114582826565 2215 80-81 0.08252381719595547 2215 82-83 0.10019981285228852 2215 84-85 0.06595954945596105 2215 86-87 0.04733191592212904 2215 88-89 0.009722966682709 2215 90-91 0.040450414663510514 2215 92-93 0.023074237578128987 2215 94-95 -0.017689196565413567 2215 96-97 -0.03467080874934325 2215 98-99 -0.03733612921383411 2215 100 0.03612143821426628 2216 1 0.028367798298916114 2216 2 0.03679248041530059 2216 3 0.019244177159571052 2216 4 0.0325659898906423 2216 5 0.016168907892449624 2216 6 0.04419069201770043 2216 7 0.039751171481107406 2216 8 0.012698439466383604 2216 9 0.004746444327267341 2216 10-11 -0.031139629411548242 2216 12-13 -0.005899130068591774 2216 14-15 -0.021510308930359656 2216 16-17 -0.02600281993621678 2216 18-19 -0.033829738706849355 2216 20-21 -0.018180040480245907 2216 22-23 -0.12177440534626527 2216 24-25 -0.06231687166761901 2216 26-27 -0.01776099576402146 2216 28-29 -0.0359761803746963 2216 30-31 -0.13394040217624337 2216 32-33 -0.051929766600316896 2216 34-35 0.032777918441098564 2216 36-37 0.007973128454601408 2216 38-39 0.0638477116368037 2216 40-41 0.0458307975516874 2216 42-43 0.07671902230603678 2216 44-45 0.0022398484812100605 2216 46-47 0.061043591789037066 2216 48-49 0.06298184617084246 2216 50-51 0.1343971938403783 2216 52-53 0.045625758044238296 2216 54-55 0.12074341915585762 2216 56-57 0.06413619017634176 2216 58-59 0.05289325944168155 2216 60-61 -0.005736052601733377 2216 62-63 0.02852026733892643 2216 64-65 0.041599421241144796 2216 66-67 0.028209336090654347 2216 68-69 0.019188983570877838 2216 70-71 0.109994197452977 2216 72-73 0.09967145453411064 2216 74-75 0.0848906882120346 2216 76-77 0.09863321809193337 2216 78-79 0.1242267608527996 2216 80-81 0.15061719243501415 2216 82-83 0.1900146893825152 2216 84-85 0.15929761683666044 2216 86-87 0.15559035047350278 2216 88-89 0.19597397715400433 2216 90-91 0.1891277013194781 2216 92-93 0.14698493669610002 2216 94-95 0.09593050390781599 2216 96-97 0.139421647024907 2216 98-99 0.13719170510476886 2216 100 0.19087864310964875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 277.0 4 70.0 5 122.0 6 302.0 7 600.0 8 1243.0 9 2251.0 10 3528.0 11 5490.0 12 8504.0 13 12291.0 14 14613.0 15 19317.0 16 24296.0 17 36329.0 18 41424.0 19 21661.0 20 13096.0 21 11467.0 22 10963.0 23 11206.0 24 12264.0 25 14328.0 26 17463.0 27 22714.0 28 28059.0 29 35438.0 30 45238.0 31 56546.0 32 72427.0 33 94151.0 34 129721.0 35 207592.0 36 342802.0 37 725661.0 38 1370301.0 39 923319.0 40 10007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.0516130635186 21.285983246849927 15.082067982490447 22.58033570714102 2 14.222575679812207 22.11997564240623 40.621463714707886 23.035984963073677 3 16.138342186794066 28.274292881007835 32.58875621222173 22.99860871997637 4 11.009593022319615 18.98690120673053 40.451627952128284 29.551877818821577 5 11.176484937861112 38.95400189539605 36.02060265125311 13.848910515489731 6 26.805368522317547 40.69735397106773 18.69065896071834 13.806618545896384 7 23.55429505249225 35.46961458143073 23.979703334485052 16.996387031591965 8 24.389400057693397 35.285285561606564 21.54599898139411 18.779315399305933 9 25.239722151421773 16.037874305631597 22.66599280994572 36.05641073300092 10-11 22.252754583611488 27.646362486594565 30.14753682784778 19.953346101946163 12-13 24.11868324240143 26.005082678349506 30.07503247841817 19.801201600830893 14-15 21.429349448226326 27.176813141121674 28.779610857079437 22.61422655357257 16-17 19.063758916109354 30.484177158772454 29.349436724450168 21.102627200668028 18-19 18.964959400080215 28.096786960637914 33.39755601100454 19.540697628277332 20-21 20.606802023777593 27.135299754918087 33.17954562079981 19.07835260050452 22-23 20.071316239166816 27.174726051165184 33.356041872813854 19.397915836854153 24-25 19.82494831055808 27.298788438127712 33.21704550787234 19.659217743441857 26-27 19.5832566061395 27.25592614774201 33.287782013412816 19.87303523270567 28-29 19.520097204820814 27.2479103643467 33.46624923986847 19.76574319096402 30-31 19.894263024321347 27.642702647680096 32.97729582256708 19.485738505431485 32-33 19.528542887081024 27.504528928205417 33.31795894001801 19.648969244695547 34-35 19.826902236236226 27.332260429469795 33.0474978497065 19.793339484587474 36-37 20.086426029486528 27.467305564437655 32.55537637995247 19.890892026123343 38-39 19.93470321998125 27.606655049659828 32.623913781105685 19.834727949253235 40-41 19.86672534162328 27.471335573747453 32.60972282063982 20.052216263989457 42-43 19.91850426209574 27.51171844852377 32.35658221225031 20.213195077130184 44-45 20.237417448047683 27.737283451009187 31.930873170710388 20.094425930232745 46-47 20.349017962847284 28.0688452550619 31.313379010407257 20.268757771683564 48-49 20.480659454060408 28.049582814885564 30.860967203275923 20.608790527778105 50-51 20.47343029063612 28.21612786908061 30.553569877866927 20.756871962416344 52-53 20.475790098422276 28.32015016959641 30.56447021394797 20.63958951803335 54-55 20.60698250721318 28.588632247757257 30.40398980694501 20.40039543808455 56-57 20.483643745074215 28.269157598006174 30.32456472500024 20.922633931919368 58-59 20.326891791669656 27.99729980658352 30.746422439255998 20.92938596249082 60-61 20.532740811218233 28.244388687821008 30.48901707749156 20.733853423469203 62-63 20.414161675086707 28.181645547271433 30.59738736696357 20.806805410678294 64-65 20.378275052601783 27.982020444446388 30.766887003906213 20.872817499045617 66-67 20.33508722007533 28.08785244991669 30.58471990682539 20.99234042318259 68-69 20.80634794734704 28.216231672289567 30.22253195574251 20.754888424620884 70-71 20.678035464078032 28.054028165424192 30.264730327300732 21.003206043197046 72-73 20.57553415666203 28.17005184709518 30.43234820746981 20.822065788772974 74-75 20.60994896580122 28.30807311106178 30.20447035197149 20.877507571165506 76-77 20.689140360230105 28.29184861340911 30.064819335726895 20.95419169063389 78-79 20.76343483218724 28.275781634417797 30.21423361030872 20.74654992308625 80-81 20.664887082927315 28.188585727812022 30.200463115044474 20.946064074216185 82-83 20.506341314841702 28.207510015992458 30.456242949238195 20.829905719927638 84-85 20.48729721780896 28.25473173302551 30.143617771967463 21.11435327719807 86-87 20.568174995358913 28.289059155411696 30.030945187772556 21.11182066145684 88-89 20.443904258587434 28.129080700109395 30.193324758195967 21.233690283107208 90-91 20.30368047988196 28.11351740678604 30.160068772775407 21.42273334055659 92-93 20.370031273974508 28.237753518753454 30.075901786607744 21.316313420664287 94-95 20.1978062980653 28.087594871133064 30.239004058125442 21.475594772676192 96-97 20.20060442420112 28.176415954268496 30.113092311275587 21.509887310254797 98-99 20.18539249658652 28.08783047738821 30.08926752687969 21.63750949914558 100 20.214404611065564 28.225224678107836 29.913542520682718 21.646828190143886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1349.0 1 842.5 2 1666.5 3 2875.5 4 1746.0 5 1058.5 6 2681.5 7 7058.0 8 13027.0 9 14906.5 10 9922.0 11 5095.5 12 4465.0 13 4924.5 14 5537.0 15 6107.0 16 6691.0 17 7596.5 18 8570.5 19 9566.5 20 11228.0 21 13814.0 22 18085.5 23 25248.5 24 32541.0 25 39637.0 26 49812.0 27 63004.0 28 80143.0 29 95608.5 30 104203.5 31 113720.5 32 124589.5 33 133875.0 34 142812.0 35 149488.5 36 150949.0 37 150594.5 38 150482.5 39 149612.5 40 149717.0 41 146496.5 42 139705.0 43 135163.0 44 132735.0 45 129354.5 46 126088.0 47 122597.0 48 118233.0 49 115234.0 50 113267.0 51 108615.0 52 100228.0 53 92737.0 54 88641.5 55 84982.0 56 78168.0 57 70235.0 58 64295.0 59 58450.0 60 51034.5 61 42771.0 62 36440.5 63 30121.0 64 22930.0 65 17869.5 66 13993.5 67 10551.5 68 7970.5 69 5990.0 70 4512.5 71 3496.5 72 2662.0 73 2023.0 74 1700.5 75 1284.5 76 762.5 77 601.0 78 460.5 79 267.5 80 137.5 81 51.5 82 29.5 83 21.5 84 12.5 85 6.5 86 3.0 87 4.0 88 3.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005382876655292081 2 0.005037820459440024 3 9.661573483857581E-4 4 0.0 5 0.042602938314533906 6 0.0 7 0.0 8 0.0 9 4.6007492780274196E-5 10-11 0.0 12-13 1.1501873195068549E-5 14-15 1.1501873195068549E-5 16-17 1.1501873195068549E-5 18-19 8.051311236547984E-5 20-21 0.0 22-23 3.450561958520565E-5 24-25 4.6007492780274196E-5 26-27 1.4952435153589114E-4 28-29 1.9553184431616536E-4 30-31 2.4153933709643953E-4 32-33 0.0014492360225786373 34-35 8.511386164350726E-4 36-37 0.0011041798267265807 38-39 9.891610947758952E-4 40-41 1.1501873195068549E-4 42-43 4.4857305460767343E-4 44-45 1.6102622473095968E-4 46-47 1.4952435153589114E-4 48-49 1.6102622473095968E-4 50-51 3.56558069047125E-4 52-53 7.591236308745242E-4 54-55 9.316517288005525E-4 56-57 0.0022083596534531614 58-59 5.060824205830162E-4 60-61 0.001173191065896992 62-63 0.0010926779535315122 64-65 0.001598760374114528 66-67 2.530412102915081E-4 68-69 2.760449566816452E-4 70-71 3.4505619585205647E-4 72-73 1.9553184431616536E-4 74-75 7.591236308745242E-4 76-77 8.166329968498669E-4 78-79 9.43153601995621E-4 80-81 7.821273772646614E-4 82-83 0.001610262247309597 84-85 0.0013457191638230202 86-87 0.0017712884720405564 88-89 0.0012537041782624719 90-91 0.0011271835731167178 92-93 0.0012191985586772662 94-95 8.511386164350726E-4 96-97 1.2652060514575404E-4 98-99 3.795618154372621E-4 100 2.3003746390137098E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4347118.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.07314808706728 #Duplication Level Percentage of deduplicated Percentage of total 1 73.86899925389815 43.63674331968988 2 16.06017679531975 18.974504042688103 3 5.001569244353269 8.863753219182056 4 1.9989552030434548 4.723383069151986 5 0.9425595069361483 2.783997866705611 6 0.5531996249925457 1.9607546021336841 7 0.34860994952404417 1.4415441021001238 8 0.22361055808903285 1.0567503689460158 9 0.17195515486388954 0.9142139094848222 >10 0.7569484891141013 7.896714688343759 >50 0.04695727590643834 1.8990353181903619 >100 0.023607516702010813 2.6318333632551743 >500 0.0016796080876165967 0.6493816018251558 >1k 0.001015576613641329 0.9438793247784337 >5k 3.906063898618929E-5 0.11763303177331416 >10k+ 1.1718191695856785E-4 1.505878171751673 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28211 0.6489586894121576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23143 0.5323757027069429 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13908 0.3199361047940268 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5098 0.11727309909691892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.3003746390137098E-5 0.0 4 0.0 0.0 0.0 2.3003746390137098E-5 0.0 5 0.0 0.0 0.0 2.3003746390137098E-5 0.0 6 0.0 0.0 0.0 2.3003746390137098E-5 0.0 7 0.0 0.0 0.0 2.3003746390137098E-5 0.0 8 0.0 0.0 0.0 2.3003746390137098E-5 0.0 9 0.0 0.0 0.0 1.380224783408226E-4 0.0 10-11 0.0 0.0 0.0 1.9553184431616533E-4 0.0 12-13 0.0 0.0 0.0 2.760449566816452E-4 0.0 14-15 0.0 0.0 0.0 2.760449566816452E-4 0.0 16-17 0.0 0.0 0.0 3.2205244946191937E-4 6.90112391704113E-5 18-19 0.0 0.0 0.0 3.4505619585205647E-4 6.90112391704113E-5 20-21 0.0 0.0 0.0 3.6805994224219356E-4 6.90112391704113E-5 22-23 0.0 0.0 0.0 5.405880401682218E-4 6.90112391704113E-5 24-25 0.0 0.0 0.0 7.361198844843871E-4 6.90112391704113E-5 26-27 0.0 0.0 0.0 0.0011041798267265807 6.90112391704113E-5 28-29 0.0 0.0 0.0 0.00221986152664823 9.201498556054839E-5 30-31 0.0 0.0 0.0 0.0047732773759534474 9.201498556054839E-5 32-33 0.0 0.0 0.0 0.010006629679709637 9.201498556054839E-5 34-35 0.0 0.0 0.0 0.018736551434766664 9.201498556054839E-5 36-37 0.0 0.0 0.0 0.03289535733789605 9.201498556054839E-5 38-39 0.0 0.0 0.0 0.05248304738909779 1.380224783408226E-4 40-41 0.0 0.0 0.0 0.07423308960097241 1.380224783408226E-4 42-43 0.0 0.0 0.0 0.0957760980953358 1.380224783408226E-4 44-45 0.0 0.0 0.0 0.1230930469336236 1.6102622473095968E-4 46-47 0.0 0.0 0.0 0.1574376402940983 1.6102622473095968E-4 48-49 0.0 0.0 0.0 0.19449667572860918 1.6102622473095968E-4 50-51 0.0 0.0 0.0 0.23290143032694305 1.6102622473095968E-4 52-53 0.0 0.0 0.0 0.27181226734585995 1.6102622473095968E-4 54-55 0.0 0.0 0.0 0.3170721383684547 1.6102622473095968E-4 56-57 0.0 0.0 0.0 0.3714759985811289 1.7252809792602823E-4 58-59 0.0 0.0 0.0 0.4350583536034679 1.8402997112109678E-4 60-61 0.0 0.0 0.0 0.5044031470965362 1.8402997112109678E-4 62-63 0.0 0.0 0.0 0.5744840604740888 1.9553184431616533E-4 64-65 0.0 0.0 0.0 0.649855835521373 2.0703371751123388E-4 66-67 0.0 0.0 0.0 0.7424574166148699 2.0703371751123388E-4 68-69 0.0 0.0 0.0 0.8494363392022024 2.0703371751123388E-4 70-71 0.0 0.0 0.0 0.9708846182689312 2.0703371751123388E-4 72-73 0.0 0.0 0.0 1.0957259499282053 2.0703371751123388E-4 74-75 0.0 0.0 0.0 1.2243283941222667 2.0703371751123388E-4 76-77 0.0 0.0 0.0 1.368998955169839 2.0703371751123388E-4 78-79 0.0 0.0 0.0 1.5368572925786694 2.0703371751123388E-4 80-81 0.0 0.0 0.0 1.7270407658591278 2.0703371751123388E-4 82-83 0.0 0.0 0.0 1.9301638464840383 2.0703371751123388E-4 84-85 0.0 0.0 0.0 2.1340805563594083 2.3003746390137098E-4 86-87 0.0 0.0 0.0 2.356124218390207 2.530412102915081E-4 88 0.0 0.0 0.0 2.5365771069476373 2.530412102915081E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11385 0.0 65.262474 1 GTATCAA 22700 0.0 45.513855 1 GTGGTAT 3575 0.0 37.094757 1 ATCAACG 29135 0.0 35.1663 3 TGGTATC 3765 0.0 34.720764 2 TCAACGC 29560 0.0 34.660297 4 TATCAAC 29885 0.0 34.380142 2 CAACGCA 30320 0.0 33.791504 5 AACGCAG 31610 0.0 32.487144 6 CGCAGAG 35300 0.0 28.851528 8 ACGCAGA 35535 0.0 28.75331 7 GCAGAGT 38115 0.0 26.535725 9 GTACATG 19090 0.0 24.683187 1 GAGTACT 26780 0.0 23.815182 12-13 TACATGG 18960 0.0 23.809067 2 CATGGGT 8265 0.0 23.609386 4 TATAACG 385 0.0 23.20611 2 CAGAGTA 37495 0.0 22.787943 10-11 ACATGGG 19175 0.0 22.756062 3 AGAGTAC 36190 0.0 22.298021 10-11 >>END_MODULE