FastQCFastQC Report
Wed 25 May 2016
SRR2431425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376461
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221040.6546499426470497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.048.7572861
GTATCAA80150.035.8151631
TCAACGC88250.031.5619494
ATCAACG89400.031.0533
TATCAAC96400.029.4818972
CAACGCA94650.029.4775165
GTACATG176600.027.6596871
AACGCAG102600.027.2078086
TACATGG178900.026.2491782
CATGGGT88700.026.0444184
ACATGGG182750.024.9491353
TGGTATC23050.024.9048752
GTGGTAT24050.024.8501641
CGCAGAG112400.024.2913368
ACGCAGA116800.023.6582437
ATGGGTG51000.022.0487795
ATGGGTA37300.020.6867315
GCAGAGT129500.020.3591869
TACCTGG24450.019.6298432
CATGGGG77500.018.7591694
Produced by FastQC (version 0.11.5)