##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431425_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3376461
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7135527405766	33.0	31.0	34.0	26.0	34.0
2	30.72783159645558	33.0	31.0	34.0	26.0	34.0
3	30.91823213714004	34.0	31.0	34.0	27.0	34.0
4	34.124286345969935	37.0	35.0	37.0	30.0	37.0
5	34.21796283149724	37.0	35.0	37.0	30.0	37.0
6	34.229617638112806	37.0	35.0	37.0	30.0	37.0
7	34.177280590535474	37.0	35.0	37.0	30.0	37.0
8	34.12524237655936	37.0	35.0	37.0	30.0	37.0
9	35.55622884434323	39.0	37.0	39.0	29.0	39.0
10-11	35.58187137360686	39.0	37.0	39.0	30.0	39.0
12-13	35.50796869858707	39.0	37.0	39.0	29.5	39.0
14-15	36.855963092717495	41.0	38.0	41.0	29.5	41.0
16-17	36.88376661243829	40.5	38.0	41.0	30.0	41.0
18-19	36.91036457995517	40.5	38.0	41.0	30.0	41.0
20-21	37.00605604507204	41.0	38.0	41.0	30.0	41.0
22-23	36.986876792002036	40.0	38.0	41.0	30.0	41.0
24-25	37.03558252264723	40.5	38.0	41.0	30.0	41.0
26-27	37.08095295636467	40.0	38.0	41.0	30.0	41.0
28-29	37.057025980753224	40.0	38.0	41.0	30.0	41.0
30-31	36.991988653208196	40.0	38.0	41.0	30.0	41.0
32-33	36.944462708143234	40.0	38.0	41.0	30.0	41.0
34-35	36.87236947205965	40.0	38.0	41.0	30.0	41.0
36-37	36.73854562513827	40.0	37.0	41.0	30.0	41.0
38-39	36.618491076899744	40.0	37.0	41.0	29.0	41.0
40-41	36.56049114738775	40.0	37.0	41.0	29.0	41.0
42-43	36.39572780494133	40.0	37.0	41.0	28.5	41.0
44-45	36.23993021095164	40.0	36.0	41.0	28.0	41.0
46-47	36.112410597960405	40.0	36.0	41.0	28.0	41.0
48-49	35.98886985515307	40.0	36.0	41.0	28.0	41.0
50-51	35.857814439438215	40.0	35.5	41.0	28.0	41.0
52-53	35.70666209383138	40.0	35.0	41.0	27.0	41.0
54-55	35.558492457043045	39.5	35.0	41.0	27.0	41.0
56-57	35.378208574006926	39.0	35.0	41.0	27.0	41.0
58-59	35.182575631704324	39.0	35.0	41.0	27.0	41.0
60-61	34.962436112841225	39.0	34.0	41.0	26.0	41.0
62-63	34.880598946648576	39.0	34.0	41.0	26.0	41.0
64-65	34.646164874997815	38.0	34.0	40.5	26.0	41.0
66-67	34.39735554475529	38.0	34.0	40.0	26.0	41.0
68-69	34.11800699015922	37.0	34.0	40.0	26.0	41.0
70-71	33.792310202901795	37.0	33.5	40.0	25.0	41.0
72-73	33.454944244876515	36.0	33.0	39.0	24.5	41.0
74-75	33.16584376363299	36.0	33.0	39.0	24.0	41.0
76-77	32.88801351474221	35.5	33.0	39.0	23.5	40.5
78-79	32.52962998832209	35.0	33.0	37.5	22.5	39.5
80-81	32.18734319750769	35.0	33.0	37.0	22.0	39.0
82-83	31.842852175695203	35.0	32.5	37.0	20.5	39.0
84-85	31.525350803696533	35.0	32.0	36.0	20.0	38.0
86-87	31.2314223383596	35.0	32.0	36.0	19.0	37.0
88-89	30.951732153873536	35.0	32.0	36.0	18.0	37.0
90-91	30.709967477782207	35.0	32.0	35.0	13.5	36.5
92-93	30.469411019407598	35.0	31.5	35.0	7.0	36.0
94-95	30.256260178926986	35.0	31.0	35.0	2.0	36.0
96-97	30.05622277289742	35.0	31.0	35.0	2.0	36.0
98-99	29.842059629890585	35.0	31.0	35.0	2.0	35.5
100	29.67665375077633	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23451906551226287
1101	2	0.32918358720278107
1101	3	0.3170396836806191
1101	4	0.4121249723133644
1101	5	0.30021352720874717
1101	6	0.3901214318688133
1101	7	0.336987625240468
1101	8	0.3307643461482641
1101	9	0.3362713736604661
1101	10-11	0.28982620272756776
1101	12-13	0.25494567960703307
1101	14-15	0.3149293674953739
1101	16-17	0.271370471329746
1101	18-19	0.27678729771854904
1101	20-21	0.27481721688150174
1101	22-23	0.3036666577478968
1101	24-25	0.2608306296238112
1101	26-27	0.26368177110887814
1101	28-29	0.2579140779937106
1101	30-31	0.3339739665914294
1101	32-33	0.32614089097526744
1101	34-35	0.3758062097592543
1101	36-37	0.35595072045722276
1101	38-39	0.43972766347712167
1101	40-41	0.3256488818404222
1101	42-43	0.31478087468161675
1101	44-45	0.2939550826217996
1101	46-47	0.3296305226499996
1101	48-49	0.43220379770628625
1101	50-51	0.36747280186170883
1101	52-53	0.30592558080792287
1101	54-55	0.24917388984783173
1101	56-57	0.17454406712596438
1101	58-59	0.22447125929505773
1101	60-61	0.21695612810228937
1101	62-63	0.18002273543764602
1101	64-65	0.2854585857164267
1101	66-67	0.24198020677963683
1101	68-69	0.2265883698645581
1101	70-71	0.27580530021959504
1101	72-73	0.18506367557401404
1101	74-75	0.16364706206702095
1101	76-77	0.2331363864614886
1101	78-79	0.2221970974315468
1101	80-81	0.16696289615125437
1101	82-83	0.2898550648823779
1101	84-85	0.25153230962130024
1101	86-87	0.24128632553873786
1101	88-89	0.2311387165361687
1101	90-91	0.2192775195119907
1101	92-93	0.15160879729164023
1101	94-95	0.09699471193706799
1101	96-97	0.20446961641739492
1101	98-99	0.1871656055112645
1101	100	0.1138276709786723
1102	1	0.12410005402920632
1102	2	0.11177772290963262
1102	3	0.1395169526614808
1102	4	0.2310334956805633
1102	5	0.17301231399235206
1102	6	0.230872715792394
1102	7	0.14870708884150474
1102	8	0.11571644379043988
1102	9	0.21783351774093518
1102	10-11	0.25942445846926177
1102	12-13	0.20159770942454713
1102	14-15	0.19085261222331695
1102	16-17	0.1301884190564735
1102	18-19	0.1431163868471046
1102	20-21	0.15051062050515895
1102	22-23	0.09260061948252485
1102	24-25	0.18388593504450057
1102	26-27	0.16063516011849543
1102	28-29	0.1133909038905827
1102	30-31	0.1368410633169006
1102	32-33	0.22420338368186776
1102	34-35	0.2129499766370202
1102	36-37	0.14895758312741947
1102	38-39	0.13568006533567
1102	40-41	0.19258101878365608
1102	42-43	0.16950900648522804
1102	44-45	0.0920898473051821
1102	46-47	0.0917026605739224
1102	48-49	0.0662937821457632
1102	50-51	0.09222610595428193
1102	52-53	0.025139891672949943
1102	54-55	-0.004840442151440527
1102	56-57	-0.05669756011357663
1102	58-59	-0.06926529186441854
1102	60-61	-0.030144915444743958
1102	62-63	0.03305632758929278
1102	64-65	-0.017711587040707855
1102	66-67	-0.004165298350663704
1102	68-69	-0.03218978012741758
1102	70-71	0.012136248096211943
1102	72-73	-0.027046593926712603
1102	74-75	3.89050476393038E-4
1102	76-77	-8.315313187452489E-4
1102	78-79	0.006497366208797928
1102	80-81	-0.059408861825303916
1102	82-83	-0.016776028361778828
1102	84-85	-0.02619658151954596
1102	86-87	-0.07706932480832407
1102	88-89	-0.005315070894262419
1102	90-91	-0.08303385335274882
1102	92-93	-0.038574643531053
1102	94-95	0.008640636521057843
1102	96-97	0.012042437339374601
1102	98-99	0.05658310528858124
1102	100	0.024247670753140937
1103	1	-0.30279170591314397
1103	2	-0.2724302029201766
1103	3	-0.19510092769508702
1103	4	-0.19167163321105107
1103	5	-0.12555581640065583
1103	6	-0.1552471638152113
1103	7	-0.14958257006221487
1103	8	-0.1286522697178043
1103	9	-0.13070852921230625
1103	10-11	-0.1324604450008664
1103	12-13	-0.13281017534509942
1103	14-15	-0.21652615899501626
1103	16-17	-0.20290129401892187
1103	18-19	-0.23387936426333766
1103	20-21	-0.3005877092834197
1103	22-23	-0.24011277219617
1103	24-25	-0.252439892500945
1103	26-27	-0.22434334166259617
1103	28-29	-0.2728292153023162
1103	30-31	-0.22867283543028805
1103	32-33	-0.2341387787710545
1103	34-35	-0.20681620797451927
1103	36-37	-0.305989587227522
1103	38-39	-0.22134859481128188
1103	40-41	-0.20786868052719853
1103	42-43	-0.2450718943463528
1103	44-45	-0.39949931132264993
1103	46-47	-0.4475761997772807
1103	48-49	-0.4301655188484972
1103	50-51	-0.5189105584969624
1103	52-53	-0.5495593028660295
1103	54-55	-0.5596306593092848
1103	56-57	-0.5482707311619848
1103	58-59	-0.5551437336455649
1103	60-61	-0.5292027902284957
1103	62-63	-0.5174810665553764
1103	64-65	-0.4573564233866918
1103	66-67	-0.558158082115618
1103	68-69	-0.5104289292768982
1103	70-71	-0.5679253201567462
1103	72-73	-0.5290204467058146
1103	74-75	-0.5764608752997944
1103	76-77	-0.5986645841081284
1103	78-79	-0.5678602735744569
1103	80-81	-0.5189817929754277
1103	82-83	-0.5421271481732894
1103	84-85	-0.6191280454734311
1103	86-87	-0.6651192055485957
1103	88-89	-0.727667999332418
1103	90-91	-0.6468713909542245
1103	92-93	-0.6258566129166141
1103	94-95	-0.6333660982256291
1103	96-97	-0.573918695755367
1103	98-99	-0.6494678636221742
1103	100	-0.6492684894329308
1104	1	-0.007226559601122062
1104	2	-0.03497698007855732
1104	3	0.01951863898056061
1104	4	0.05533512600422341
1104	5	0.09332989733337627
1104	6	0.04981640062661796
1104	7	-0.009360637631971258
1104	8	0.0820650941422656
1104	9	0.02125796083468856
1104	10-11	0.07335752637191462
1104	12-13	0.04959547771038331
1104	14-15	-0.003683578922895947
1104	16-17	0.0394549365117598
1104	18-19	0.025962280704455054
1104	20-21	-0.04102589765022202
1104	22-23	0.04724509290723233
1104	24-25	0.03789119394617302
1104	26-27	0.008334847453163263
1104	28-29	0.08876815553906425
1104	30-31	0.05546143993407071
1104	32-33	0.017354341353694736
1104	34-35	0.020275783906363642
1104	36-37	-0.04591452142875596
1104	38-39	-0.07370404713321221
1104	40-41	-0.026313916405946713
1104	42-43	-0.09105761191709405
1104	44-45	-0.048123954828234616
1104	46-47	-0.2111603935634605
1104	48-49	-0.2403903292301237
1104	50-51	-0.24166175880664298
1104	52-53	-0.27924036433273614
1104	54-55	-0.1912480562787522
1104	56-57	-0.2552469881049433
1104	58-59	-0.40185111236014137
1104	60-61	-0.27226672262681717
1104	62-63	-0.21282669698011603
1104	64-65	-0.19213479396468358
1104	66-67	-0.10141071132856894
1104	68-69	-0.1630155831556408
1104	70-71	-0.22241894072573132
1104	72-73	-0.21519420575749848
1104	74-75	-0.19786670648232274
1104	76-77	-0.16730650368079125
1104	78-79	-0.2199137792293442
1104	80-81	-0.1798645199258857
1104	82-83	-0.32961282006293047
1104	84-85	-0.16854453101082711
1104	86-87	-0.20222749634723414
1104	88-89	-0.25716553811141196
1104	90-91	-0.31149892361203513
1104	92-93	-0.3082563102928795
1104	94-95	-0.28791524244259037
1104	96-97	-0.3055417265883982
1104	98-99	-0.37524390116209716
1104	100	-0.32933136635951143
1105	1	-0.16230321279092408
1105	2	-0.20175367788608156
1105	3	-0.086356329051398
1105	4	0.032299440384200295
1105	5	0.019435627574019065
1105	6	-0.025355804899170664
1105	7	0.005462027478749576
1105	8	-0.03882905223274946
1105	9	-0.009247668001655995
1105	10-11	-0.06160547425236729
1105	12-13	-0.052649889679400985
1105	14-15	-0.12404644468061576
1105	16-17	-0.13476566681902824
1105	18-19	-0.053006340421625
1105	20-21	-0.06334787335148917
1105	22-23	-0.14005633866126033
1105	24-25	-0.15239168790652258
1105	26-27	-0.1244634054279743
1105	28-29	-0.07991930358703314
1105	30-31	-0.08746299379987477
1105	32-33	-0.06476554379587895
1105	34-35	-0.13064658534253937
1105	36-37	-0.12850846217838097
1105	38-39	-0.1767450842333389
1105	40-41	-0.149980053370669
1105	42-43	-0.2864550494854683
1105	44-45	-0.26868852894815376
1105	46-47	-0.21417832557170158
1105	48-49	-0.35463595025474604
1105	50-51	-0.2825679879948382
1105	52-53	-0.257804207908201
1105	54-55	-0.26433815633422597
1105	56-57	-0.37347188833756206
1105	58-59	-0.28831123897322897
1105	60-61	-0.3477526627068599
1105	62-63	-0.1855070146225941
1105	64-65	-0.1861886865708442
1105	66-67	-0.20757186649050396
1105	68-69	-0.20723705799427705
1105	70-71	-0.12531788936959742
1105	72-73	-0.11181525772249756
1105	74-75	-0.12228815576720109
1105	76-77	-0.0856112666263158
1105	78-79	-0.09929097084719984
1105	80-81	-0.05524743842988755
1105	82-83	-3.7717298448214365E-4
1105	84-85	-0.05609968842015789
1105	86-87	-0.04760552042674249
1105	88-89	-0.01698469547541137
1105	90-91	-0.08745423292100796
1105	92-93	-0.11020075309431476
1105	94-95	-0.12043378969731933
1105	96-97	-0.15218037622855007
1105	98-99	-0.12847403313039152
1105	100	-0.12085895334701746
1106	1	-0.19856562668035949
1106	2	-0.22944204780656108
1106	3	-0.22128969162278622
1106	4	-0.16675861240270962
1106	5	-0.19953297556669725
1106	6	-0.1980219570313011
1106	7	-0.20682048194326796
1106	8	-0.20440113777254965
1106	9	-0.23373012014810968
1106	10-11	-0.28597521592426034
1106	12-13	-0.23010980275886794
1106	14-15	-0.22071143377132785
1106	16-17	-0.21082177073864017
1106	18-19	-0.2742999706104712
1106	20-21	-0.2798686990120913
1106	22-23	-0.13262922038562408
1106	24-25	-0.13441787323106524
1106	26-27	-0.25076905806744065
1106	28-29	-0.24266362714649148
1106	30-31	-0.23107255292303108
1106	32-33	-0.2751276523909212
1106	34-35	-0.24954816778326006
1106	36-37	-0.3155142233609425
1106	38-39	-0.21057434232670147
1106	40-41	-0.22255667458075834
1106	42-43	-0.23961498266078252
1106	44-45	-0.256534682377513
1106	46-47	-0.2882316571881489
1106	48-49	-0.2555148768143738
1106	50-51	-0.2747381160297948
1106	52-53	-0.35403743898802276
1106	54-55	-0.3060993991343466
1106	56-57	-0.32101446532690403
1106	58-59	-0.3894019170002423
1106	60-61	-0.37314720116125244
1106	62-63	-0.31615060617353663
1106	64-65	-0.24802799163367695
1106	66-67	-0.22093271913468726
1106	68-69	-0.20789218513758811
1106	70-71	-0.19784166135929837
1106	72-73	-0.21715423218387286
1106	74-75	-0.1724361694413048
1106	76-77	-0.2357634516636864
1106	78-79	-0.21418370187021196
1106	80-81	-0.2827836910428658
1106	82-83	-0.2290295939608491
1106	84-85	-0.24419994440720316
1106	86-87	-0.3184330137186997
1106	88-89	-0.4160216683358122
1106	90-91	-0.28313387987148175
1106	92-93	-0.25829243607325836
1106	94-95	-0.27466996646877817
1106	96-97	-0.2018973888726805
1106	98-99	-0.20626459945889053
1106	100	-0.1716568351391068
1107	1	-0.23438746969795332
1107	2	-0.3037129408708772
1107	3	-0.2644473392405615
1107	4	-0.16228268461546946
1107	5	-0.04043258657941351
1107	6	-0.1666260344665318
1107	7	-0.16745695668824823
1107	8	-0.10322870224945291
1107	9	-0.1016150843614696
1107	10-11	-0.12986371491961535
1107	12-13	-0.12981431756057304
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2216	64-65	0.5181910367423228
2216	66-67	0.49478650360091336
2216	68-69	0.5371561404578387
2216	70-71	0.506269294110524
2216	72-73	0.48977298580365414
2216	74-75	0.5166043001090017
2216	76-77	0.5069318714308579
2216	78-79	0.46917645270616504
2216	80-81	0.44405025224815375
2216	82-83	0.45961561263951367
2216	84-85	0.4767712117810028
2216	86-87	0.42761579671981664
2216	88-89	0.42959639908323055
2216	90-91	0.4269743049018615
2216	92-93	0.4680181217945183
2216	94-95	0.4941529015879844
2216	96-97	0.4231114107477367
2216	98-99	0.40216193945392575
2216	100	0.4047884433173614
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80341.0
3	16763.0
4	11887.0
5	10502.0
6	6982.0
7	5657.0
8	6313.0
9	6730.0
10	6897.0
11	7214.0
12	8225.0
13	9175.0
14	9944.0
15	10239.0
16	10291.0
17	10608.0
18	10316.0
19	9691.0
20	9671.0
21	9993.0
22	11773.0
23	17741.0
24	28557.0
25	37838.0
26	43197.0
27	33951.0
28	30497.0
29	37225.0
30	44986.0
31	50564.0
32	63473.0
33	85208.0
34	120156.0
35	187779.0
36	311247.0
37	574807.0
38	932307.0
39	505870.0
40	1846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.515007027520525	22.15385418777618	14.46007665145249	21.87106213325081
2	14.455031001132761	23.13965017881503	40.369533265173764	22.035785554878444
3	16.871886904539355	29.28720685048655	31.166592186794333	22.67431405817976
4	11.518049654091195	19.905815249657532	39.8505873343704	28.725547761880872
5	11.68867626103373	40.82775303911637	34.96976462761143	12.513806072238474
6	26.75270901503296	41.12127732726324	19.163376724996443	12.962636932707355
7	23.98332717325163	36.0838715624447	23.587580208265102	16.345221056038564
8	23.206109313514258	38.123374086984455	21.403668599140907	17.266848000360373
9	24.38121547943687	19.223941883063194	21.917516339017045	34.477326298482886
10-11	21.54665831265825	29.99682278960851	29.694380220205574	18.762138677527666
12-13	22.502402163339994	28.22082534835073	29.12237014239894	20.154402345910334
14-15	20.517150602223065	29.821952278865904	26.613933959441617	23.046963159469414
16-17	19.620399282093594	32.76110348473856	27.353718864247405	20.26477836892044
18-19	19.692670000500875	31.596683908831203	29.17398838739774	19.536657703270183
20-21	21.599820512626728	30.529865634910653	28.320772032808613	19.549541819654014
22-23	21.0982998163069	30.61410933740671	28.410592316898164	19.876998529388228
24-25	20.661706782591583	30.671848632154347	28.44728046096514	20.21916412428893
26-27	20.378851028484586	30.597855523867317	28.53331377208328	20.48997967556481
28-29	20.300010491742643	30.63526612571953	28.682142283441834	20.382581099095994
30-31	20.568817599014313	30.990693689649657	28.31497200489583	20.125516706440198
32-33	20.253505585418647	30.754501655819777	28.73291516604063	20.259077592720942
34-35	20.508249375446983	30.47303018690207	28.63547058058164	20.38324985706931
36-37	20.6643658965519	30.628209243807824	28.307638633848832	20.39978622579145
38-39	20.488993663388815	30.68004937449732	28.519700225842826	20.311256736271034
40-41	20.40275177802727	30.481401105643098	28.653951257334104	20.461895858995533
42-43	20.321152011912787	30.432600225143112	28.6813833675079	20.5648643954362
44-45	20.54990773608816	30.508985541615104	28.573162688325837	20.367944033970904
46-47	20.528900154023695	30.688299045165714	28.413185424346178	20.369615376464413
48-49	20.475186756626815	30.39239196097274	28.504550868171545	20.627870414228898
50-51	20.446061173722732	30.392789631130324	28.492235278076862	20.668913917070086
52-53	20.457777240961793	30.44838872459191	28.476029643110408	20.617804391335888
54-55	20.615791241993826	30.656608149242775	28.331951823570655	20.395648785192744
56-57	20.52270424215013	30.41694465249129	28.250077875549096	20.810273229809486
58-59	20.466911145731814	30.218344585061402	28.54481306006692	20.769931209139866
60-61	20.644813547104356	30.55987145758468	28.20130427514384	20.594010720167123
62-63	20.476244811587073	30.477963785952127	28.423863513435414	20.621927889025386
64-65	20.553625608437194	30.29437053147098	28.48708644572502	20.66491741436681
66-67	20.392486953532124	30.361907499931462	28.334668297768452	20.910937248767965
68-69	20.789158860943605	30.481167644984613	28.166576691349494	20.56309680272229
70-71	20.67613058025411	30.276862371674586	28.303023055489984	20.74398399258132
72-73	20.523510681931874	30.406624078029903	28.495721774260467	20.57414346577775
74-75	20.573683304244174	30.449075592993584	28.396326259762205	20.580914843000034
76-77	20.627751054926105	30.443935905069843	28.210088937747134	20.718224102256922
78-79	20.694735513080527	30.388232459342024	28.39616711138299	20.520864916194462
80-81	20.6221630468869	30.30221609127809	28.43355431210945	20.64206654972556
82-83	20.531469053807598	30.318803180918046	28.590870733188627	20.55885703208573
84-85	20.480563680534395	30.27374525889206	28.395976030950145	20.849715029623404
86-87	20.703238002873903	30.33226846340989	28.330761560823852	20.633731972892353
88-89	20.56064863076817	30.149806461824564	28.423657570663885	20.865887336743384
90-91	20.498076057812522	30.151398926196617	28.366217104619658	20.984307911371207
92-93	20.638234372744783	30.246618304441363	28.309402656277	20.805744666536853
94-95	20.481332997837352	30.1420908453165	28.45423784471693	20.922338312129217
96-97	20.481414951437955	30.251172373854413	28.349752840504106	20.91765983420353
98-99	20.423826851980568	30.261585799069124	28.308969659497418	21.005617689452894
100	20.540670167068967	30.333002620114264	28.156046049271428	20.970281163545344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20200.0
1	14687.5
2	7831.0
3	5397.5
4	3682.5
5	2677.0
6	2167.5
7	2044.0
8	2110.5
9	2145.5
10	1821.5
11	1462.5
12	1417.0
13	1512.5
14	1576.0
15	1646.0
16	1905.5
17	2311.5
18	2967.5
19	3918.0
20	5337.0
21	7611.0
22	11348.0
23	16863.0
24	23100.5
25	29423.5
26	38046.0
27	49271.0
28	63065.0
29	76309.5
30	84719.0
31	93086.0
32	101568.5
33	108916.0
34	115973.0
35	120136.0
36	121020.0
37	121083.0
38	120845.5
39	119976.0
40	118647.5
41	114754.5
42	108740.0
43	103910.5
44	100153.0
45	96372.0
46	94315.0
47	91750.0
48	87898.5
49	85748.0
50	84339.0
51	81189.5
52	77042.0
53	72787.5
54	69941.5
55	67786.5
56	62314.5
57	55646.5
58	50656.5
59	46306.0
60	40648.0
61	33824.0
62	28195.0
63	22807.0
64	17509.5
65	13983.0
66	11272.5
67	8604.5
68	6499.0
69	4929.0
70	3707.0
71	2815.0
72	2171.0
73	1678.0
74	1373.5
75	1139.0
76	834.5
77	678.5
78	542.0
79	382.5
80	286.5
81	201.0
82	149.5
83	131.0
84	110.5
85	85.0
86	71.0
87	58.5
88	52.0
89	51.0
90	44.0
91	37.5
92	36.5
93	31.5
94	23.5
95	21.5
96	18.5
97	11.5
98	12.5
99	16.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18184720629084714
2	0.1758942277135735
3	0.1902287631931777
4	0.17959632881884316
5	0.1944935836664484
6	0.07525631126792225
7	0.07664830128350364
8	0.06524582987927299
9	0.05991480428768465
10-11	0.07202807910412706
12-13	0.07164306058917903
14-15	0.06922929066854319
16-17	0.06530506349695732
18-19	0.07013260333822899
20-21	0.07130246728749422
22-23	0.06974758482328094
24-25	0.06879984694033191
26-27	0.07236867240581189
28-29	0.07045838823549272
30-31	0.0760411567022394
32-33	0.0726352236853913
34-35	0.07250194804560159
36-37	0.0831639992287783
38-39	0.07861781907150713
40-41	0.07395317167886731
42-43	0.07721102065150463
44-45	0.08843579120268233
46-47	0.07787739885045318
48-49	0.08348978412604204
50-51	0.08021712674898362
52-53	0.07873628630687575
54-55	0.08104639739656404
56-57	0.0736866203992879
58-59	0.08165354197782826
60-61	0.07798105768140073
62-63	0.0697772016321231
64-65	0.07250194804560159
66-67	0.07296100858265503
68-69	0.07071013111065105
70-71	0.06739304852032942
72-73	0.06412039114327102
74-75	0.06965873439675446
76-77	0.07487129275297419
78-79	0.07108034122117803
80-81	0.07946189812350861
82-83	0.08049848643298413
84-85	0.08224587815467141
86-87	0.07857339385824388
88-89	0.08020231834456254
90-91	0.07875109471129683
92-93	0.06930333269064859
94-95	0.06965873439675446
96-97	0.06882946374917406
98-99	0.07860301066708604
100	0.08719188523131172
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3376461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.25827288518764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9601922217802	51.65507690412395
2	14.15177624793287	18.753445048913058
3	4.072047487781591	8.094205009406261
4	1.5885448103737168	4.210169421443615
5	0.7434676106568527	2.463043991410009
6	0.4118390315069967	1.6372645760617186
7	0.22965428693400305	1.065154748904845
8	0.16688759545137744	0.8846147072456132
9	0.10801052066665882	0.6440931497522411
>10	0.5215477382173945	5.948108736166291
>50	0.027733598148057398	1.2749075251746438
>100	0.017179486639435505	2.0908893768422065
>500	8.954911299661227E-4	0.41681921138982003
>1k	1.7909822490207137E-4	0.2064515927757192
>5k	0.0	0.0
>10k+	4.477455622551784E-5	0.6557560003900701
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22104	0.6546499426470497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.9616808842157513E-5	0.0
3	0.0	0.0	0.0	2.9616808842157513E-5	0.0
4	0.0	0.0	0.0	2.9616808842157513E-5	0.0
5	0.0	0.0	0.0	2.9616808842157513E-5	0.0
6	0.0	0.0	0.0	2.9616808842157513E-5	0.0
7	0.0	0.0	0.0	2.9616808842157513E-5	0.0
8	0.0	0.0	0.0	8.885042652647254E-5	0.0
9	0.0	0.0	0.0	8.885042652647254E-5	0.0
10-11	0.0	1.4808404421078756E-5	0.0	8.885042652647254E-5	0.0
12-13	0.0	2.9616808842157513E-5	0.0	8.885042652647254E-5	0.0
14-15	0.0	2.9616808842157513E-5	0.0	8.885042652647254E-5	0.0
16-17	0.0	2.9616808842157513E-5	0.0	8.885042652647254E-5	0.0
18-19	0.0	2.9616808842157513E-5	0.0	1.0365883094755129E-4	0.0
20-21	0.0	2.9616808842157513E-5	0.0	1.6289244863186634E-4	0.0
22-23	0.0	2.9616808842157513E-5	0.0	3.554017061058902E-4	0.0
24-25	0.0	5.9233617684315026E-5	0.0	6.959950077907015E-4	0.0
26-27	0.0	5.9233617684315026E-5	0.0	0.0012883311846338518	0.0
28-29	0.0	5.9233617684315026E-5	0.0	0.0025914707736887825	0.0
30-31	0.0	5.9233617684315026E-5	0.0	0.006115871025905526	0.0
32-33	0.0	5.9233617684315026E-5	0.0	0.012542718544653707	0.0
34-35	0.0	5.9233617684315026E-5	0.0	0.022553199933302945	2.9616808842157513E-5
36-37	0.0	5.9233617684315026E-5	0.0	0.03742083797206602	2.9616808842157513E-5
38-39	0.0	5.9233617684315026E-5	0.0	0.05825626299252383	2.9616808842157513E-5
40-41	0.0	5.9233617684315026E-5	0.0	0.08069099569045815	4.442521326323627E-5
42-43	0.0	5.9233617684315026E-5	0.0	0.10436963435976307	5.9233617684315026E-5
44-45	0.0	5.9233617684315026E-5	0.0	0.13317198095876126	5.9233617684315026E-5
46-47	0.0	5.9233617684315026E-5	0.0	0.17199961735082975	5.9233617684315026E-5
48-49	0.0	5.9233617684315026E-5	0.0	0.2129596639795336	5.9233617684315026E-5
50-51	0.0	1.036588309475513E-4	0.0	0.25387528539497417	5.9233617684315026E-5
52-53	0.0	1.4808404421078758E-4	0.0	0.2954128597961001	5.9233617684315026E-5
54-55	0.0	1.4808404421078758E-4	0.0	0.34374749182650116	5.9233617684315026E-5
56-57	0.0	1.4808404421078758E-4	0.0	0.4024183901428152	5.9233617684315026E-5
58-59	0.0	1.4808404421078758E-4	0.0	0.46901178482440636	5.9233617684315026E-5
60-61	0.0	1.4808404421078758E-4	0.0	0.542831680863484	5.9233617684315026E-5
62-63	0.0	1.4808404421078758E-4	0.0	0.6189172627789866	5.9233617684315026E-5
64-65	0.0	1.4808404421078758E-4	0.0	0.6994749828296551	5.9233617684315026E-5
66-67	0.0	1.4808404421078758E-4	0.0	0.7986616756420406	5.9233617684315026E-5
68-69	0.0	1.7770085305294508E-4	0.0	0.9148484167298245	5.9233617684315026E-5
70-71	0.0	1.7770085305294508E-4	0.0	1.0424968628395233	5.9233617684315026E-5
72-73	0.0	1.7770085305294508E-4	0.0	1.1777716372260778	5.9233617684315026E-5
74-75	0.0	2.5174287515833887E-4	0.0	1.311861739258946	5.9233617684315026E-5
76-77	0.0	2.6655127957941763E-4	0.0	1.4597236574034174	5.9233617684315026E-5
78-79	0.0	2.6655127957941763E-4	0.0	1.6409192938997372	5.9233617684315026E-5
80-81	0.0	2.6655127957941763E-4	0.0	1.8431428646739885	5.9233617684315026E-5
82-83	0.0	2.6655127957941763E-4	0.0	2.0590938263465803	5.9233617684315026E-5
84-85	0.0	2.6655127957941763E-4	0.0	2.2766885209099117	5.9233617684315026E-5
86-87	0.0	2.6655127957941763E-4	0.0	2.515414808582122	5.9233617684315026E-5
88	0.0	2.6655127957941763E-4	0.0	2.7123665873824696	5.9233617684315026E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4710	0.0	48.757286	1
GTATCAA	8015	0.0	35.815163	1
TCAACGC	8825	0.0	31.561949	4
ATCAACG	8940	0.0	31.053	3
TATCAAC	9640	0.0	29.481897	2
CAACGCA	9465	0.0	29.477516	5
GTACATG	17660	0.0	27.659687	1
AACGCAG	10260	0.0	27.207808	6
TACATGG	17890	0.0	26.249178	2
CATGGGT	8870	0.0	26.044418	4
ACATGGG	18275	0.0	24.949135	3
TGGTATC	2305	0.0	24.904875	2
GTGGTAT	2405	0.0	24.850164	1
CGCAGAG	11240	0.0	24.291336	8
ACGCAGA	11680	0.0	23.658243	7
ATGGGTG	5100	0.0	22.048779	5
ATGGGTA	3730	0.0	20.686731	5
GCAGAGT	12950	0.0	20.359186	9
TACCTGG	2445	0.0	19.629843	2
CATGGGG	7750	0.0	18.759169	4
>>END_MODULE