FastQCFastQC Report
Wed 25 May 2016
SRR2431425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376461
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280500.8307514880225183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237080.7021553040298704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144100.42677821541548977No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.1408871596621433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45840.13576345173245005No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.1344010785257108No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.10605779246376605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100150.065.546861
GTATCAA194950.044.4066431
GTGGTAT30300.037.2465441
TGGTATC31850.034.989442
ATCAACG250550.034.1931573
TATCAAC253200.033.984862
TCAACGC252400.033.9239044
CAACGCA260300.032.8762635
AACGCAG271100.031.5867316
TATAACG3400.030.4257932
ATAACGC3950.028.5692863
ACGCAGA304900.028.008087
CGCAGAG304200.027.948938
GCAGAGT325500.025.903819
GTACATG170150.024.292621
CATGGGT83250.024.060834
TACATGG171850.023.7502562
GAGTACT225450.023.65083712-13
ACATGGG170650.023.1726073
CAGAGTA321650.022.72161110-11
Produced by FastQC (version 0.11.5)