##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431425_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3376461
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.634562045881765	34.0	31.0	34.0	31.0	34.0
2	32.75717356131168	34.0	31.0	34.0	31.0	34.0
3	32.8627133557888	34.0	31.0	34.0	31.0	34.0
4	36.33135966919209	37.0	37.0	37.0	35.0	37.0
5	36.23094802516599	37.0	37.0	37.0	35.0	37.0
6	36.27498318505678	37.0	37.0	37.0	35.0	37.0
7	36.23421624002172	37.0	37.0	37.0	35.0	37.0
8	36.24860586276578	37.0	37.0	37.0	35.0	37.0
9	38.04510373435381	39.0	39.0	39.0	35.0	39.0
10-11	38.02539641358214	39.0	39.0	39.0	35.0	39.0
12-13	38.0072761095123	39.0	39.0	39.0	35.0	39.0
14-15	39.46701339064778	41.0	39.0	41.0	36.0	41.0
16-17	39.49595449199621	41.0	39.0	41.0	36.5	41.0
18-19	39.577154600630664	41.0	40.0	41.0	37.0	41.0
20-21	39.57804251255975	41.0	40.0	41.0	37.0	41.0
22-23	39.5193828686308	41.0	40.0	41.0	36.0	41.0
24-25	39.469731621363316	41.0	39.0	41.0	36.0	41.0
26-27	39.35561450287742	41.0	39.0	41.0	36.0	41.0
28-29	39.27227147003919	41.0	39.0	41.0	36.0	41.0
30-31	39.085557777803444	41.0	39.0	41.0	35.0	41.0
32-33	38.854458854996395	41.0	38.0	41.0	35.0	41.0
34-35	38.716520048654495	41.0	38.0	41.0	35.0	41.0
36-37	38.57172110088048	41.0	38.0	41.0	35.0	41.0
38-39	38.423072412209116	41.0	38.0	41.0	35.0	41.0
40-41	38.28234607181898	40.5	38.0	41.0	34.5	41.0
42-43	38.10124180317794	40.0	38.0	41.0	34.0	41.0
44-45	37.84934121851252	40.0	38.0	41.0	33.5	41.0
46-47	37.472004859525995	40.0	38.0	41.0	33.0	41.0
48-49	37.1279412082651	40.0	37.5	41.0	32.0	41.0
50-51	36.96089544644526	40.0	37.0	41.0	31.0	41.0
52-53	36.90561315531262	40.0	37.0	41.0	31.0	41.0
54-55	36.77592514766201	40.0	37.0	41.0	31.0	41.0
56-57	36.632286882626516	40.0	36.0	41.0	31.0	41.0
58-59	36.4658005823257	40.0	36.0	41.0	31.0	41.0
60-61	36.32354557034718	40.0	35.0	41.0	31.0	41.0
62-63	36.17172729079353	39.0	35.0	41.0	31.0	41.0
64-65	35.94974767959707	39.0	35.0	41.0	30.0	41.0
66-67	35.70029684927503	39.0	35.0	41.0	30.0	41.0
68-69	35.41790546373851	38.0	35.0	41.0	29.0	41.0
70-71	35.126880630340466	37.0	35.0	40.5	29.0	41.0
72-73	34.81660427885885	37.0	35.0	40.0	29.0	41.0
74-75	34.482336831374624	36.5	35.0	39.0	29.0	41.0
76-77	34.14524808668011	36.0	35.0	39.0	29.0	41.0
78-79	33.8160873174605	36.0	35.0	38.5	28.5	40.0
80-81	33.54052541995894	35.0	34.5	37.0	29.0	39.0
82-83	33.32248958302791	35.0	35.0	37.0	29.0	39.0
84-85	33.04509588589947	35.0	34.0	36.5	29.0	39.0
86-87	32.788314303052815	35.0	34.0	36.0	29.0	37.0
88-89	32.54620370263421	35.0	34.0	36.0	28.0	37.0
90-91	32.352334589382195	35.0	34.0	36.0	27.5	37.0
92-93	32.183705068709514	35.0	34.0	35.0	27.0	36.0
94-95	32.0395890845474	35.0	34.0	35.0	27.0	36.0
96-97	31.894576155329503	35.0	34.0	35.0	27.0	36.0
98-99	31.777317433845674	35.0	34.0	35.0	26.0	36.0
100	31.674175712380507	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.02406743121293431
1101	2	0.029191447404883775
1101	3	0.026541601469666887
1101	4	0.059361356625075246
1101	5	0.031143781868962606
1101	6	0.04505650787695714
1101	7	0.02452192031144307
1101	8	0.06550466767914997
1101	9	0.03237817102813523
1101	10-11	0.076340669493149
1101	12-13	0.0445110060837024
1101	14-15	0.0924884780392361
1101	16-17	0.03595909452499768
1101	18-19	-0.01707436416954522
1101	20-21	0.07606361174678256
1101	22-23	0.05476038740986411
1101	24-25	0.10344875800423381
1101	26-27	0.10629537894288887
1101	28-29	0.0315212840857626
1101	30-31	0.07455166907207911
1101	32-33	0.12208161460792866
1101	34-35	0.11114556741361525
1101	36-37	0.10427330488031572
1101	38-39	0.09590095770757046
1101	40-41	0.0746665808468947
1101	42-43	0.05046051228438131
1101	44-45	0.016642178150867437
1101	46-47	0.03814200081924213
1101	48-49	0.06410899692171768
1101	50-51	-0.009853570945821843
1101	52-53	0.024007041133756957
1101	54-55	-0.0057052323266404414
1101	56-57	-0.006691025175278753
1101	58-59	-0.09382274696201165
1101	60-61	-0.060792412903509785
1101	62-63	-0.06781538711935298
1101	64-65	-0.03448362313864095
1101	66-67	-0.09328035393071588
1101	68-69	-0.053864273928390105
1101	70-71	0.006033091743972818
1101	72-73	-0.04780862518158102
1101	74-75	-0.08814687970143353
1101	76-77	-0.04118552389425645
1101	78-79	-0.017678923953234005
1101	80-81	-0.038479401540328695
1101	82-83	-0.04915539111641465
1101	84-85	-0.0037065798287798657
1101	86-87	0.018261859321292206
1101	88-89	0.019113478516516125
1101	90-91	-0.05137307467478536
1101	92-93	-0.039728130888271096
1101	94-95	-0.009380535805028956
1101	96-97	-0.04292210159805521
1101	98-99	0.02680470555951686
1101	100	0.02103111121374468
1102	1	-0.005811658935584774
1102	2	0.018555658002689768
1102	3	0.01245642164688121
1102	4	0.05628929079573908
1102	5	0.06729788893564148
1102	6	0.009154795231296475
1102	7	0.039370111782510264
1102	8	0.029321038574273928
1102	9	0.06467452059643364
1102	10-11	0.027774952739235914
1102	12-13	0.0018167539231725982
1102	14-15	-0.0018495117937789018
1102	16-17	-0.011392607597898063
1102	18-19	-0.0030689445315061903
1102	20-21	0.0676491973392217
1102	22-23	0.08394437926836673
1102	24-25	0.08752852376396447
1102	26-27	0.09897334330079133
1102	28-29	0.041523299946824466
1102	30-31	-0.003893454590425449
1102	32-33	0.05985384612811373
1102	34-35	0.06680340268292184
1102	36-37	0.06614876014372584
1102	38-39	0.06069351977575366
1102	40-41	-0.010881253787630385
1102	42-43	0.00837627665999463
1102	44-45	0.00396322296747087
1102	46-47	0.023484815668297188
1102	48-49	-0.010502569711491105
1102	50-51	0.026620970604163574
1102	52-53	0.012264111946990397
1102	54-55	-0.005856833694487307
1102	56-57	0.026333112321900387
1102	58-59	0.08775673022929453
1102	60-61	-0.004729935654800954
1102	62-63	0.010872754866184664
1102	64-65	0.05013616785293351
1102	66-67	0.10593740074975955
1102	68-69	0.06073071221589288
1102	70-71	0.022057730102176265
1102	72-73	0.008040799246366248
1102	74-75	0.05244112074304752
1102	76-77	0.026652128715987544
1102	78-79	0.049970509825477905
1102	80-81	0.01970106021728668
1102	82-83	0.07512098634958164
1102	84-85	0.04203351042756509
1102	86-87	0.025369321694313385
1102	88-89	-0.031566421625505825
1102	90-91	-0.00985241856079
1102	92-93	0.01509708154052447
1102	94-95	0.017830927767121807
1102	96-97	-0.004797620213473408
1102	98-99	0.007525674277687244
1102	100	0.02641729938518722
1103	1	-0.09758814716141018
1103	2	-0.10394126015875571
1103	3	-0.054874338616663465
1103	4	-0.012120504193582349
1103	5	-0.11093393045851485
1103	6	-0.027755830900680678
1103	7	-0.028119213124604414
1103	8	-0.008749998216870836
1103	9	-0.030750138696831186
1103	10-11	0.032380729060143665
1103	12-13	0.01706038221720263
1103	14-15	0.016240730394365244
1103	16-17	0.020760931193599674
1103	18-19	-0.09261178181255048
1103	20-21	-0.09471541472047562
1103	22-23	-0.08153776848444494
1103	24-25	-0.045033929092767266
1103	26-27	-0.09791058864896485
1103	28-29	-0.036402192350081464
1103	30-31	-0.08691181569096784
1103	32-33	-0.07770250480246688
1103	34-35	-0.031133590520326493
1103	36-37	-0.10125303911031125
1103	38-39	-0.08918978358298801
1103	40-41	-0.09919093269358825
1103	42-43	0.019368202763210718
1103	44-45	-0.01659327252804843
1103	46-47	-0.018433031520388
1103	48-49	-0.037755429013941466
1103	50-51	-0.011045555050358757
1103	52-53	0.0527635478561308
1103	54-55	0.06693376953380437
1103	56-57	0.03524142964439392
1103	58-59	-0.018175236952679086
1103	60-61	0.027593164528020964
1103	62-63	0.0035616517122107894
1103	64-65	-0.031840674349737697
1103	66-67	-0.01800821204273717
1103	68-69	0.042348548670055663
1103	70-71	0.06794013747973793
1103	72-73	0.01828075927911499
1103	74-75	0.026212883412590315
1103	76-77	0.09189828626200125
1103	78-79	0.11803949022478122
1103	80-81	0.13038457407069615
1103	82-83	0.06218166310465989
1103	84-85	0.045181604390890584
1103	86-87	0.044511275919589366
1103	88-89	0.042865467352683595
1103	90-91	0.05171598243679654
1103	92-93	0.02772496420049464
1103	94-95	0.005426448267300543
1103	96-97	0.0706730173280512
1103	98-99	0.08956891991872595
1103	100	0.13378882543855752
1104	1	-0.13779153130790434
1104	2	-0.11500921082249249
1104	3	-0.07179285598103746
1104	4	-0.013451692535063842
1104	5	-0.0581013300150417
1104	6	-0.026064459798000428
1104	7	-0.08207925498906121
1104	8	-0.0830051141035213
1104	9	-0.044468303919657615
1104	10-11	-0.11202091993995822
1104	12-13	-0.04671117096793154
1104	14-15	-0.08542843960797342
1104	16-17	-0.06964395970211967
1104	18-19	-0.06929866212412605
1104	20-21	-0.06904840163527837
1104	22-23	-0.0691548038501466
1104	24-25	-0.06252908158782589
1104	26-27	-0.13157616250770587
1104	28-29	-0.10891465560145264
1104	30-31	-0.07199162722569241
1104	32-33	-0.04371724680119371
1104	34-35	-0.05984238840012779
1104	36-37	-0.16665621853189094
1104	38-39	-0.1857171336083283
1104	40-41	-0.1683285110567425
1104	42-43	-0.11568587187846191
1104	44-45	-0.07484083920469686
1104	46-47	-0.08204229767120808
1104	48-49	-0.12197174229219598
1104	50-51	-0.15245410954557315
1104	52-53	-0.05863881106093771
1104	54-55	-0.014198964709741801
1104	56-57	-0.06366549633825969
1104	58-59	-0.07340076661317596
1104	60-61	-0.08387965947785148
1104	62-63	-0.09332642439945005
1104	64-65	-0.10496857868372444
1104	66-67	-0.07917897235770255
1104	68-69	-0.16454088073908935
1104	70-71	-0.18501423466169342
1104	72-73	-0.1653485455380661
1104	74-75	-0.083261883356748
1104	76-77	-0.08364563024782257
1104	78-79	-0.08439559266280838
1104	80-81	-0.1265005672494226
1104	82-83	-0.06582657136369363
1104	84-85	-0.08920996885381527
1104	86-87	-0.09360017276824095
1104	88-89	-0.09336672422610093
1104	90-91	-0.15638700542506712
1104	92-93	-0.16850386210607127
1104	94-95	-0.1829966767866651
1104	96-97	-0.19351292992711322
1104	98-99	-0.20848990062608763
1104	100	-0.15577500234877917
1105	1	-0.1849373761822335
1105	2	-0.19455918187081522
1105	3	-0.16162575107180288
1105	4	-0.08935659336660962
1105	5	-0.08641069288406555
1105	6	-0.02706687995917889
1105	7	-0.02171780217430097
1105	8	-0.07502124183819348
1105	9	-0.06211019559383857
1105	10-11	-0.062300962670725824
1105	12-13	-0.08732310597635973
1105	14-15	-0.10101984744225234
1105	16-17	-0.14735460246749454
1105	18-19	-0.15170394851620728
1105	20-21	-0.07215369124123328
1105	22-23	-0.12681538889208355
1105	24-25	-0.16173825542610132
1105	26-27	-0.10950902116223915
1105	28-29	-0.11580761263917339
1105	30-31	-0.05069599989121798
1105	32-33	-0.10003450196591501
1105	34-35	-0.046461930220274894
1105	36-37	-0.03369725166123061
1105	38-39	-0.054275612054055955
1105	40-41	-0.026997712362195614
1105	42-43	-0.040750197802715604
1105	44-45	0.009671204707061065
1105	46-47	0.03227454957654885
1105	48-49	0.044033813766830576
1105	50-51	0.04686848440096014
1105	52-53	-0.007365071520013089
1105	54-55	0.06842239937666506
1105	56-57	0.07138395190875002
1105	58-59	0.007872779764873883
1105	60-61	0.03923985138246877
1105	62-63	0.00941713360850116
1105	64-65	0.04596744018981269
1105	66-67	0.03900628191970412
1105	68-69	0.11327836753967802
1105	70-71	0.03233523241766534
1105	72-73	0.06429935743080506
1105	74-75	0.045262092974148516
1105	76-77	0.0021204212247525334
1105	78-79	0.08434117056199142
1105	80-81	0.06341726986910601
1105	82-83	0.013731692865654566
1105	84-85	0.016485630701609466
1105	86-87	-0.00891498543603575
1105	88-89	0.052034061636561546
1105	90-91	0.07192337909768298
1105	92-93	0.013895058597427123
1105	94-95	0.08740280098696473
1105	96-97	0.06293648669413088
1105	98-99	0.057771982974959
1105	100	0.06133596856184553
1106	1	-0.12999717528428079
1106	2	-0.11035266440853775
1106	3	-0.064768050575843
1106	4	-0.014350820391079822
1106	5	-0.052912215531264906
1106	6	-0.06412727125934481
1106	7	-0.042549160053049206
1106	8	-0.05129355544439562
1106	9	-0.052622953750230295
1106	10-11	-0.07854133088537196
1106	12-13	-0.09853901334867743
1106	14-15	-0.1796150247247894
1106	16-17	-0.12948090847869764
1106	18-19	-0.12399292892984448
1106	20-21	-0.09285771696136891
1106	22-23	-0.09412455041699275
1106	24-25	-0.06994103212772274
1106	26-27	-0.10882437176313431
1106	28-29	-0.10469164666857012
1106	30-31	-0.1921134418789876
1106	32-33	-0.15684008313044018
1106	34-35	-0.22951394022772575
1106	36-37	-0.24268275061603362
1106	38-39	-0.20710870477370236
1106	40-41	-0.24242480019923107
1106	42-43	-0.2748836586381955
1106	44-45	-0.36116251176486713
1106	46-47	-0.42921241274189725
1106	48-49	-0.2657906670597896
1106	50-51	-0.3298100012610945
1106	52-53	-0.3037809700635421
1106	54-55	-0.2897786601735106
1106	56-57	-0.2835528067180846
1106	58-59	-0.3043494181985409
1106	60-61	-0.303914220370082
1106	62-63	-0.3919529739640666
1106	64-65	-0.35332616912229753
1106	66-67	-0.3235664021928031
1106	68-69	-0.36421629096391683
1106	70-71	-0.29976562309826704
1106	72-73	-0.2863393631471425
1106	74-75	-0.29681284271833164
1106	76-77	-0.346239806710976
1106	78-79	-0.3224291990375221
1106	80-81	-0.2566920716419503
1106	82-83	-0.28929627463102037
1106	84-85	-0.27751304848621317
1106	86-87	-0.23508584367869645
1106	88-89	-0.260502502533825
1106	90-91	-0.3012434920069538
1106	92-93	-0.28556892321463323
1106	94-95	-0.31002295791030576
1106	96-97	-0.28002220429940294
1106	98-99	-0.26481597302835524
1106	100	-0.18483689528182978
1107	1	-0.25415608293182146
1107	2	-0.20781401300322955
1107	3	-0.1382906168312985
1107	4	-0.19504127514615277
1107	5	-0.2736960700314697
1107	6	-0.2432548611353198
1107	7	-0.14722117861577289
1107	8	-0.17211282599167532
1107	9	-0.10860581573277983
1107	10-11	-0.1411215558299901
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2216	30-31	-0.11800740651693076
2216	32-33	-0.07842060183318722
2216	34-35	-0.019460281102368526
2216	36-37	-0.04792747157975441
2216	38-39	-0.020703753537674174
2216	40-41	-0.06509648143359215
2216	42-43	-0.05121629875343103
2216	44-45	-0.005945642399737494
2216	46-47	-0.020069613525684815
2216	48-49	0.014057165424105733
2216	50-51	0.05782959751716987
2216	52-53	-0.026537521236946304
2216	54-55	0.04376464810234637
2216	56-57	0.0033952954885805298
2216	58-59	0.003910820687792693
2216	60-61	-0.024068851779894374
2216	62-63	-0.07123767233932199
2216	64-65	-0.08376349297789432
2216	66-67	-0.07386845316352719
2216	68-69	-0.050045859019668626
2216	70-71	-0.03368879658825108
2216	72-73	-0.04685732544431431
2216	74-75	-0.07411681693410088
2216	76-77	-0.0658168310364502
2216	78-79	-0.03082576164339912
2216	80-81	-0.006094465420297013
2216	82-83	-0.004418819104948568
2216	84-85	0.03861241912561297
2216	86-87	0.028444980655670804
2216	88-89	0.06134126241353499
2216	90-91	0.07903458431794519
2216	92-93	0.024494101906718413
2216	94-95	-0.013390457449844462
2216	96-97	-0.04185983767125023
2216	98-99	-0.03989689113787165
2216	100	-0.031665358489242124
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	249.0
4	66.0
5	133.0
6	274.0
7	614.0
8	1147.0
9	1826.0
10	2938.0
11	4266.0
12	6691.0
13	9696.0
14	11204.0
15	15186.0
16	19414.0
17	29629.0
18	34865.0
19	18152.0
20	10680.0
21	9233.0
22	9042.0
23	9055.0
24	9696.0
25	11412.0
26	13699.0
27	17656.0
28	22086.0
29	27619.0
30	35192.0
31	44351.0
32	56037.0
33	72813.0
34	101047.0
35	168458.0
36	267259.0
37	547614.0
38	1049316.0
39	730454.0
40	7352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.33431192312739	21.334531098807656	15.088320394563615	22.242836583501344
2	14.195585932039133	22.208094314005645	40.82636711947057	22.769952634484657
3	16.015115377814844	28.587777204851633	32.84689238245495	22.550215034878565
4	10.953895217507325	19.084953150650932	40.31567371872502	29.645477913116725
5	11.057374921627865	39.02514545512729	35.972365599936474	13.945114023308378
6	26.45850196403868	40.76140669179949	18.745722222172862	14.034369121988972
7	23.30040240358174	35.904279658494495	23.909412843803025	16.885905094120737
8	24.41630452713655	35.34514392436341	21.503906012834147	18.734645535665894
9	25.417589600472212	15.999859023989913	22.709813618460277	35.87273775707761
10-11	22.268078914579494	27.79691517242462	30.008461522286208	19.92654439070968
12-13	24.150464050969344	26.270761012788242	30.138790289596116	19.439984646646298
14-15	21.40078662267582	27.34095768941436	29.12143191389799	22.136823774011834
16-17	18.748550627417288	30.67210016641685	29.724273433041283	20.855075773124582
18-19	18.667195031005864	27.98137871106349	34.0996837514342	19.251742506496452
20-21	20.36841237023025	26.981223831698337	33.95008856906684	18.70027522900457
22-23	19.863321328154534	27.091188269843013	34.06818592078478	18.977304481217676
24-25	19.516549877682543	27.18437357469065	34.033469965585255	19.265606582041546
26-27	19.281871393871057	27.194486290583043	34.06066389423349	19.462978421312403
28-29	19.239090419874785	27.231191019941303	34.185192238377546	19.344526321806367
30-31	19.501542445688617	27.557222721101652	33.8090737743954	19.132161058814333
32-33	19.202904023463912	27.391090975379534	34.10115865359128	19.304846347565274
34-35	19.502120281788084	27.25622386669028	33.83851561912287	19.40314023239876
36-37	19.69774849237796	27.41250253781624	33.4105007172524	19.4792482525534
38-39	19.58105189427006	27.527960354605046	33.427515580424014	19.46347217070088
40-41	19.555978549714144	27.44129684993907	33.38883033805983	19.613894262286948
42-43	19.57165971015128	27.474617579227417	33.13396225800404	19.81976045261726
44-45	19.905445292078266	27.657192833436696	32.668598276655594	19.76876359782944
46-47	19.98008860762118	28.0238853781432	32.040474947274646	19.95555106696098
48-49	20.148306214200673	27.994674896193057	31.547893355763136	20.309125533843137
50-51	20.1497043940181	28.165643601245566	31.300307956216788	20.384344048519544
52-53	20.12646446666869	28.363314346386765	31.179991958992343	20.330229227952202
54-55	20.290349976343443	28.583146314501136	31.021052483494095	20.105451225661326
56-57	20.167341103549138	28.27198638074847	30.990875776752556	20.569796738949833
58-59	20.044540857329316	27.947633172765308	31.3910717778922	20.616754192013182
60-61	20.205027689074413	28.231142315741337	31.15801283133447	20.40581716384978
62-63	20.095212822040757	28.11910250927309	31.334578828089736	20.451105840596416
64-65	20.05501974870455	27.93650915880233	31.519693616678474	20.488777475814647
66-67	20.01793005855553	28.044430057222776	31.280029664266024	20.657610219955675
68-69	20.462023038956538	28.17012221397886	30.941860620088228	20.42599412697637
70-71	20.335289725638116	28.014842795087034	30.99339750916124	20.65646997011361
72-73	20.252397705642334	28.116188063404923	31.099966621816893	20.53144760913585
74-75	20.309186141044798	28.25489531148886	30.858328804408185	20.57758974305816
76-77	20.35199770172304	28.236849879718072	30.7615781995399	20.64957421901898
78-79	20.394637664118914	28.26279402648767	30.846522031051055	20.496046278342362
80-81	20.30502841824946	28.2036891228171	30.86488000155193	20.62640245738151
82-83	20.158416263848615	28.16788842386657	31.079966035041245	20.59372927724357
84-85	20.177720281143145	28.180434390826164	30.826602480519732	20.81524284751096
86-87	20.2717211185987	28.21736623264462	30.69553604827386	20.81537660048282
88-89	20.120710371815903	28.066442386319984	30.844451341935518	20.968395899928595
90-91	20.004421825574234	28.102049100034105	30.737418640112413	21.15611043427925
92-93	20.007584941122673	28.215536719733613	30.71975197449849	21.057126364645228
94-95	19.881446208760583	28.051288901156894	30.889972343668276	21.177292546414243
96-97	19.90258424809919	28.10911796273963	30.73100908171171	21.25728870744947
98-99	19.849910564442837	28.05756098817887	30.72433842195665	21.368190025421644
100	19.889126415906848	28.139813970735013	30.602572281367042	21.368487331991098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1819.0
1	1098.5
2	2115.0
3	3752.5
4	2256.5
5	1236.5
6	3208.0
7	8451.5
8	15270.5
9	17269.0
10	11137.0
11	5078.5
12	3955.0
13	4249.5
14	4738.5
15	5218.0
16	5708.0
17	6241.5
18	7036.0
19	7963.5
20	9239.0
21	11322.5
22	14647.5
23	19742.0
24	25427.5
25	31356.0
26	39416.0
27	50030.5
28	63452.5
29	75830.5
30	83842.0
31	92234.0
32	100284.5
33	107304.0
34	114005.5
35	117714.5
36	119090.0
37	120040.5
38	119813.5
39	118342.0
40	117141.5
41	113619.5
42	107686.0
43	102892.5
44	99485.0
45	96193.0
46	93851.5
47	91123.0
48	87623.0
49	85143.5
50	83758.5
51	80309.5
52	74927.5
53	70729.5
54	67658.0
55	64809.0
56	59236.0
57	53036.0
58	48575.0
59	44087.5
60	38319.5
61	31810.5
62	26535.5
63	21334.5
64	16192.0
65	12471.5
66	9840.5
67	7405.5
68	5438.0
69	4040.0
70	2896.5
71	2173.5
72	1604.0
73	1200.0
74	1012.0
75	788.5
76	496.5
77	371.0
78	294.0
79	161.5
80	78.0
81	38.0
82	18.0
83	10.0
84	7.5
85	3.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	1.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005153324738535407
2	0.004886773458955989
3	9.477378829490403E-4
4	0.0
5	0.0455210351903961
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.961680884215751E-5
16-17	0.0
18-19	8.885042652647254E-5
20-21	0.0
22-23	4.442521326323627E-5
24-25	2.961680884215751E-5
26-27	1.3327563978970882E-4
28-29	5.923361768431502E-5
30-31	1.4808404421078755E-4
32-33	0.0012439059713706155
34-35	6.811866033696228E-4
36-37	0.0010217799050544342
38-39	8.440790520014891E-4
40-41	7.404202210539378E-5
42-43	2.961680884215751E-4
44-45	8.885042652647254E-5
46-47	5.923361768431502E-5
48-49	2.961680884215751E-5
50-51	2.073176618951026E-4
52-53	5.47910963579914E-4
54-55	5.47910963579914E-4
56-57	0.0018954757658980806
58-59	3.1097649284265387E-4
60-61	0.001155055544844143
62-63	7.404202210539378E-4
64-65	0.001095821927159828
66-67	2.3693447073726008E-4
68-69	1.7770085305294508E-4
70-71	2.3693447073726008E-4
72-73	1.1846723536863004E-4
74-75	4.7386894147452015E-4
76-77	5.47910963579914E-4
78-79	6.515697945274653E-4
80-81	6.367613901063865E-4
82-83	0.0011846723536863004
84-85	9.329294785279616E-4
86-87	0.0017177749128451359
88-89	9.477378829490403E-4
90-91	8.144622431593316E-4
92-93	0.0010069715006333555
94-95	5.627193680009927E-4
96-97	1.4808404421078755E-4
98-99	2.2212606631618134E-4
100	8.885042652647254E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3376461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.67783017301369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3942238919038	46.50152137231014
2	15.717586831743313	19.38853302675721
3	4.578842433258803	8.472391979625757
4	1.7513981084744987	4.320897403993102
5	0.863295614277321	2.6623100143252065
6	0.438487908410747	1.622698964872682
7	0.28191106233989116	1.2171363838825577
8	0.18302152382133674	0.903069637140687
9	0.13438521502732073	0.7459729623197113
>10	0.5942636672559971	6.403757211852384
>50	0.0372484081321784	1.5908586248647814
>100	0.02312273634102531	2.6576835527687637
>500	0.00134679940244351	0.5849446947019453
>1k	7.21499676079671E-4	0.9652165931125299
>5k	0.0	0.0
>10k+	1.4429993521593418E-4	1.9630075774725646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28050	0.8307514880225183	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23708	0.7021553040298704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14410	0.42677821541548977	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4757	0.1408871596621433	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4584	0.13576345173245005	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4538	0.1344010785257108	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3581	0.10605779246376605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9616808842157513E-5	0.0
5	0.0	0.0	0.0	2.9616808842157513E-5	0.0
6	0.0	0.0	0.0	2.9616808842157513E-5	0.0
7	0.0	0.0	0.0	2.9616808842157513E-5	0.0
8	0.0	0.0	0.0	2.9616808842157513E-5	0.0
9	0.0	0.0	0.0	2.9616808842157513E-5	0.0
10-11	0.0	0.0	0.0	2.9616808842157513E-5	0.0
12-13	0.0	0.0	0.0	2.9616808842157513E-5	0.0
14-15	0.0	0.0	0.0	2.9616808842157513E-5	0.0
16-17	0.0	0.0	0.0	8.885042652647254E-5	0.0
18-19	0.0	0.0	0.0	1.0365883094755129E-4	0.0
20-21	0.0	1.4808404421078756E-5	0.0	1.6289244863186634E-4	0.0
22-23	0.0	2.9616808842157513E-5	0.0	3.702101105269689E-4	0.0
24-25	0.0	2.9616808842157513E-5	0.0	6.959950077907015E-4	0.0
26-27	0.0	2.9616808842157513E-5	0.0	0.001273522780212773	0.0
28-29	0.0	2.9616808842157513E-5	0.0	0.0025914707736887825	0.0
30-31	0.0	2.9616808842157513E-5	0.0	0.006115871025905526	0.0
32-33	0.0	2.9616808842157513E-5	0.0	0.012572335353495865	0.0
34-35	0.0	2.9616808842157513E-5	0.0	0.0228641764261456	0.0
36-37	0.0	4.442521326323627E-5	0.0	0.038027982553330245	0.0
38-39	0.0	5.9233617684315026E-5	0.0	0.059159575662209626	0.0
40-41	0.0	5.9233617684315026E-5	0.0	0.0816979671910915	0.0
42-43	0.0	5.9233617684315026E-5	0.0	0.10564315713997585	0.0
44-45	0.0	8.885042652647254E-5	0.0	0.13448992895223727	0.0
46-47	0.0	8.885042652647254E-5	0.0	0.17376181747693814	0.0
48-49	0.0	1.1846723536863005E-4	0.0	0.2151364994294322	0.0
50-51	0.0	1.4808404421078758E-4	0.0	0.25639271414655757	0.0
52-53	0.0	1.4808404421078758E-4	0.0	0.29871513398200067	0.0
54-55	0.0	1.4808404421078758E-4	0.0	0.3479678870865086	0.0
56-57	0.0	1.4808404421078758E-4	0.0	0.4076309484990349	0.0
58-59	0.0	1.4808404421078758E-4	0.0	0.475334973512207	0.0
60-61	0.0	1.4808404421078758E-4	0.0	0.5503395419049709	0.0
62-63	0.0	1.4808404421078758E-4	0.0	0.627831922240476	0.0
64-65	0.0	1.4808404421078758E-4	0.0	0.7095150810271464	0.0
66-67	0.0	1.4808404421078758E-4	0.0	0.8101678058772188	0.0
68-69	0.0	1.4808404421078758E-4	0.0	0.9276873033628998	0.0
70-71	0.0	1.4808404421078758E-4	0.0	1.057305267260602	0.0
72-73	0.0	1.4808404421078758E-4	0.0	1.1948753443324238	0.0
74-75	0.0	1.7770085305294508E-4	0.0	1.3313644078815066	0.0
76-77	0.0	1.7770085305294508E-4	0.0	1.4818770304173512	0.0
78-79	0.0	1.7770085305294508E-4	0.0	1.666138006628834	0.0
80-81	0.0	2.073176618951026E-4	0.0	1.8716786599934072	0.0
82-83	0.0	2.073176618951026E-4	0.0	2.0908430454253732	0.0
84-85	0.0	2.073176618951026E-4	0.0	2.312598901631027	0.0
86-87	0.0	2.073176618951026E-4	0.0	2.5562563879754574	0.0
88	0.0	2.073176618951026E-4	0.0	2.757413753631391	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10015	0.0	65.54686	1
GTATCAA	19495	0.0	44.406643	1
GTGGTAT	3030	0.0	37.246544	1
TGGTATC	3185	0.0	34.98944	2
ATCAACG	25055	0.0	34.193157	3
TATCAAC	25320	0.0	33.98486	2
TCAACGC	25240	0.0	33.923904	4
CAACGCA	26030	0.0	32.876263	5
AACGCAG	27110	0.0	31.586731	6
TATAACG	340	0.0	30.425793	2
ATAACGC	395	0.0	28.569286	3
ACGCAGA	30490	0.0	28.00808	7
CGCAGAG	30420	0.0	27.94893	8
GCAGAGT	32550	0.0	25.90381	9
GTACATG	17015	0.0	24.29262	1
CATGGGT	8325	0.0	24.06083	4
TACATGG	17185	0.0	23.750256	2
GAGTACT	22545	0.0	23.650837	12-13
ACATGGG	17065	0.0	23.172607	3
CAGAGTA	32165	0.0	22.721611	10-11
>>END_MODULE