FastQCFastQC Report
Wed 25 May 2016
SRR2431424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3563750
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA202320.5677165906699404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54250.051.428391
GTATCAA97700.039.295571
ATCAACG115200.032.1808363
TCAACGC116250.031.9693164
TATCAAC124550.030.4449272
CAACGCA123950.029.9833225
AACGCAG132400.028.2882356
GTACATG220550.027.3055521
CATGGGT95800.027.0082454
TACATGG220200.026.5996322
CGCAGAG144850.025.467528
ACGCAGA148800.024.9181447
ACATGGG228300.024.8934233
TACCTGG25250.024.7807162
ATGGGTG54750.024.2305985
GTGGTAT26450.023.6577821
GCAGAGT160500.022.2821649
GTACCTG29850.021.9087961
TGGTATC27800.020.815362
CAGAGTA156800.019.01661710-11
Produced by FastQC (version 0.11.5)