##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431424_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3563750 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.564600210452472 31.0 31.0 34.0 26.0 34.0 2 30.567770747106277 33.0 31.0 34.0 26.0 34.0 3 30.766302350052612 34.0 31.0 34.0 26.0 34.0 4 33.95149772009821 37.0 35.0 37.0 28.0 37.0 5 34.06879775517363 37.0 35.0 37.0 30.0 37.0 6 34.0613976850228 37.0 35.0 37.0 30.0 37.0 7 34.010624482637674 37.0 35.0 37.0 30.0 37.0 8 33.94440070150824 37.0 35.0 37.0 30.0 37.0 9 35.34118751315328 39.0 37.0 39.0 28.0 39.0 10-11 35.36509323044545 39.0 37.0 39.0 28.0 39.0 12-13 35.290828902139594 39.0 37.0 39.0 27.0 39.0 14-15 36.61058491757278 40.0 38.0 41.0 27.0 41.0 16-17 36.64057607856893 40.0 38.0 41.0 28.0 41.0 18-19 36.67395285864609 40.0 38.0 41.0 28.0 41.0 20-21 36.770563170817255 40.0 38.0 41.0 28.5 41.0 22-23 36.75304819361628 40.0 38.0 41.0 29.0 41.0 24-25 36.80645878639074 40.0 38.0 41.0 29.0 41.0 26-27 36.85494184496668 40.0 38.0 41.0 30.0 41.0 28-29 36.83010733076114 40.0 38.0 41.0 30.0 41.0 30-31 36.75849287969133 40.0 37.0 41.0 30.0 41.0 32-33 36.70903219922834 40.0 37.0 41.0 30.0 41.0 34-35 36.640591371448615 40.0 37.0 41.0 30.0 41.0 36-37 36.50477446509996 40.0 37.0 41.0 29.0 41.0 38-39 36.375646299544016 40.0 37.0 41.0 28.5 41.0 40-41 36.31305941774816 40.0 37.0 41.0 28.0 41.0 42-43 36.140664608909155 40.0 36.0 41.0 27.5 41.0 44-45 35.97420526131182 40.0 36.0 41.0 27.0 41.0 46-47 35.83653931953701 40.0 35.5 41.0 27.0 41.0 48-49 35.6965254296738 40.0 35.0 41.0 27.0 41.0 50-51 35.55086818660119 40.0 35.0 41.0 27.0 41.0 52-53 35.38135629603647 39.5 35.0 41.0 27.0 41.0 54-55 35.208078148018245 39.0 35.0 41.0 26.0 41.0 56-57 35.00498477727113 39.0 34.5 41.0 26.0 41.0 58-59 34.77551525780428 39.0 34.0 41.0 26.0 41.0 60-61 34.52330789196773 38.0 34.0 40.5 25.0 41.0 62-63 34.42037264117853 38.0 34.0 40.0 25.0 41.0 64-65 34.17190740091196 38.0 34.0 40.0 24.5 41.0 66-67 33.90919579095055 37.0 33.5 40.0 23.5 41.0 68-69 33.62303725008769 37.0 33.0 40.0 23.0 41.0 70-71 33.30273882848123 36.0 33.0 39.5 22.5 41.0 72-73 32.972187583304105 36.0 33.0 39.0 22.0 41.0 74-75 32.69915285864609 35.0 33.0 39.0 22.0 41.0 76-77 32.4460725359523 35.0 33.0 38.5 20.5 40.0 78-79 32.10858505787443 35.0 32.0 37.0 20.0 39.5 80-81 31.790998947737634 35.0 32.0 37.0 19.5 39.0 82-83 31.470108312872675 35.0 32.0 36.5 18.0 39.0 84-85 31.169673798667134 35.0 32.0 36.0 17.5 37.5 86-87 30.889110768151525 35.0 32.0 36.0 13.5 37.0 88-89 30.622570326201334 35.0 31.0 35.0 7.5 37.0 90-91 30.390487127323745 35.0 31.0 35.0 4.5 36.0 92-93 30.159938407576288 35.0 31.0 35.0 2.0 36.0 94-95 29.945935882146614 35.0 31.0 35.0 2.0 36.0 96-97 29.743146404770258 35.0 31.0 35.0 2.0 36.0 98-99 29.521993546124165 34.0 31.0 35.0 2.0 35.0 100 29.348829463346195 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3613132239882155 1101 2 0.3113719514031743 1101 3 0.3656082743998894 1101 4 0.36406880503663785 1101 5 0.3672094818250855 1101 6 0.3746717033460101 1101 7 0.377797413483151 1101 8 0.35591296635506353 1101 9 0.332880246013211 1101 10-11 0.3196824007963386 1101 12-13 0.3006765307702395 1101 14-15 0.3931811913646399 1101 16-17 0.2779428234308128 1101 18-19 0.27088393139153766 1101 20-21 0.2699232842521866 1101 22-23 0.2889567395379373 1101 24-25 0.30215368077992366 1101 26-27 0.33645646364423243 1101 28-29 0.36985831832711824 1101 30-31 0.38928837528615645 1101 32-33 0.3906586807958661 1101 34-35 0.3493292279459226 1101 36-37 0.3330550334207487 1101 38-39 0.38870360037296336 1101 40-41 0.33747442101156366 1101 42-43 0.43929407849314117 1101 44-45 0.4177608760145901 1101 46-47 0.4143636526842158 1101 48-49 0.39912592499038624 1101 50-51 0.43749831724603894 1101 52-53 0.3613223243859949 1101 54-55 0.36480030821696374 1101 56-57 0.26617463318197565 1101 58-59 0.2579007969823195 1101 60-61 0.31018775946284194 1101 62-63 0.25766866630074503 1101 64-65 0.3243080170444159 1101 66-67 0.2477285218308367 1101 68-69 0.2776519560458368 1101 70-71 0.2531353457695218 1101 72-73 0.22653442596295292 1101 74-75 0.22829543198434266 1101 76-77 0.3046193367572414 1101 78-79 0.29102005836041656 1101 80-81 0.25091772316370964 1101 82-83 0.29627059812664314 1101 84-85 0.23496805380361963 1101 86-87 0.25350552059710907 1101 88-89 0.212325762018704 1101 90-91 0.27131888560841233 1101 92-93 0.24462259672288056 1101 94-95 0.22395400344615268 1101 96-97 0.2521453520106469 1101 98-99 0.2470478892366046 1101 100 0.35976757882639987 1102 1 0.10427097046709122 1102 2 0.1556958876833292 1102 3 0.20485997959963953 1102 4 0.21377962235142434 1102 5 0.20179034224968717 1102 6 0.22754655201875806 1102 7 0.14196773414928998 1102 8 0.23938267875946906 1102 9 0.16921244254670853 1102 10-11 0.21647075671803861 1102 12-13 0.12656253336864154 1102 14-15 0.19646960634087662 1102 16-17 0.20601670434734132 1102 18-19 0.19177438153474213 1102 20-21 0.07683386461064146 1102 22-23 0.12716714716187738 1102 24-25 0.14360332202361548 1102 26-27 0.15709491700591371 1102 28-29 0.12960001009196276 1102 30-31 0.14968058968365483 1102 32-33 0.19180821426501637 1102 34-35 0.23151358144637868 1102 36-37 0.18591096618715852 1102 38-39 0.15744649537399624 1102 40-41 0.2414189872559831 1102 42-43 0.2012812267495505 1102 44-45 0.15437348932547934 1102 46-47 0.07236683424738999 1102 48-49 0.05610534079337981 1102 50-51 0.0456174856105207 1102 52-53 0.0986671216812951 1102 54-55 0.1799314646524266 1102 56-57 0.17157664694168773 1102 58-59 0.17032476426610543 1102 60-61 0.052866380982315775 1102 62-63 0.16718650446178884 1102 64-65 0.14638223383362003 1102 66-67 0.21608728552411094 1102 68-69 0.09818890942227654 1102 70-71 0.12866037751133774 1102 72-73 0.1010804442108082 1102 74-75 0.06620456919755924 1102 76-77 0.099133539373355 1102 78-79 0.11298077971158449 1102 80-81 0.05838793753168048 1102 82-83 0.08986438042209599 1102 84-85 0.10519155677111058 1102 86-87 0.05702184917655373 1102 88-89 0.0670958922339544 1102 90-91 0.09865679935926153 1102 92-93 0.07642005073416058 1102 94-95 0.05296851240767708 1102 96-97 0.07282255991214726 1102 98-99 0.026660048565744887 1102 100 0.016062792456185804 1103 1 -0.3611208265839707 1103 2 -0.35748237387025483 1103 3 -0.24815069108576893 1103 4 -0.1659386116006374 1103 5 -0.09445253413996113 1103 6 -0.17859088400390277 1103 7 -0.18597349993762435 1103 8 -0.2145356789863868 1103 9 -0.18518671713757584 1103 10-11 -0.1524647973108486 1103 12-13 -0.16633314970222557 1103 14-15 -0.19226521099948712 1103 16-17 -0.2143676676572781 1103 18-19 -0.2531477606386119 1103 20-21 -0.34993753552881657 1103 22-23 -0.28545046226321347 1103 24-25 -0.23362335669442302 1103 26-27 -0.19646599221642447 1103 28-29 -0.23606509533322395 1103 30-31 -0.31751908355052194 1103 32-33 -0.2557756578568373 1103 34-35 -0.28709960301853243 1103 36-37 -0.41288062819559457 1103 38-39 -0.36682687283177273 1103 40-41 -0.3138402637186317 1103 42-43 -0.3471724213407512 1103 44-45 -0.4180636320191766 1103 46-47 -0.5325514864453709 1103 48-49 -0.4863549873330797 1103 50-51 -0.5576901072074918 1103 52-53 -0.6799835426018177 1103 54-55 -0.6305450981987448 1103 56-57 -0.5701974129725684 1103 58-59 -0.5156678275435809 1103 60-61 -0.5206278167148142 1103 62-63 -0.5585999210134958 1103 64-65 -0.5325338232791808 1103 66-67 -0.5847324459806202 1103 68-69 -0.583287304309728 1103 70-71 -0.5068256877895863 1103 72-73 -0.5563221122101396 1103 74-75 -0.6151451856445576 1103 76-77 -0.5325187828841322 1103 78-79 -0.5681152980659121 1103 80-81 -0.5841423928604854 1103 82-83 -0.6570271264302967 1103 84-85 -0.639748777866977 1103 86-87 -0.6931931802897715 1103 88-89 -0.6909972489334955 1103 90-91 -0.7042396331257379 1103 92-93 -0.6972119116040965 1103 94-95 -0.7455358748919743 1103 96-97 -0.6819048234222223 1103 98-99 -0.7603593393170378 1103 100 -0.7375776746315488 1104 1 -0.25440575617249195 1104 2 -0.15151940979036382 1104 3 -0.19221579324062432 1104 4 -0.2289676655441184 1104 5 -0.14093041319733857 1104 6 -0.1830308834911918 1104 7 -0.1520167004517603 1104 8 -0.19658229475827227 1104 9 -0.16385282864548856 1104 10-11 -0.20418159474587583 1104 12-13 -0.22041196901676585 1104 14-15 -0.23678828175835065 1104 16-17 -0.24388824195811054 1104 18-19 -0.27939952760063136 1104 20-21 -0.30317256659936476 1104 22-23 -0.2744858152555807 1104 24-25 -0.28537823347597424 1104 26-27 -0.27477783127210387 1104 28-29 -0.2252942456671576 1104 30-31 -0.21847270399017304 1104 32-33 -0.3214405574400203 1104 34-35 -0.21875758337145612 1104 36-37 -0.3286007204096464 1104 38-39 -0.26972678136286277 1104 40-41 -0.2657885193052607 1104 42-43 -0.3045713112399895 1104 44-45 -0.32060279573020267 1104 46-47 -0.41991240574282784 1104 48-49 -0.4509542182797617 1104 50-51 -0.4802588950806097 1104 52-53 -0.6304820924289487 1104 54-55 -0.4501750050672726 1104 56-57 -0.47033202850737865 1104 58-59 -0.558464790299027 1104 60-61 -0.6125663443176492 1104 62-63 -0.46493020317846145 1104 64-65 -0.5269460622128435 1104 66-67 -0.42399662819227046 1104 68-69 -0.42231293940801606 1104 70-71 -0.5229518052399698 1104 72-73 -0.4876175540151948 1104 74-75 -0.4263247100323966 1104 76-77 -0.4613962609237703 1104 78-79 -0.5393530035098699 1104 80-81 -0.5442979994949333 1104 82-83 -0.5744562862336231 1104 84-85 -0.553065087976055 1104 86-87 -0.4761576278946684 1104 88-89 -0.5410075236305794 1104 90-91 -0.6301710253541088 1104 92-93 -0.7105554714280764 1104 94-95 -0.6692311051417903 1104 96-97 -0.6921138625921159 1104 98-99 -0.7674536262080345 1104 100 -0.7094037041107946 1105 1 -0.25397909358620296 1105 2 -0.21191668295657706 1105 3 -0.19894436297186857 1105 4 -0.06408129377985006 1105 5 -0.11351017646112638 1105 6 -0.13666016608391374 1105 7 -0.06184092899807325 1105 8 -0.11126483355567984 1105 9 -0.12279292101010952 1105 10-11 -0.2016877171699747 1105 12-13 -0.11776708849939155 1105 14-15 -0.1509202522101205 1105 16-17 -0.23031741998372723 1105 18-19 -0.1843839406799077 1105 20-21 -0.15007107123937402 1105 22-23 -0.1825310500400832 1105 24-25 -0.28110928414773895 1105 26-27 -0.20003451656961602 1105 28-29 -0.18288994381384782 1105 30-31 -0.1955386327093791 1105 32-33 -0.14270421328224359 1105 34-35 -0.18377805440219674 1105 36-37 -0.21892782194872495 1105 38-39 -0.24743746241851028 1105 40-41 -0.31366601327577825 1105 42-43 -0.32996303262519433 1105 44-45 -0.338879991910531 1105 46-47 -0.3412622863129968 1105 48-49 -0.44763807561586333 1105 50-51 -0.41148123561988115 1105 52-53 -0.4012813637604751 1105 54-55 -0.49826683406795524 1105 56-57 -0.523466836783939 1105 58-59 -0.4742604362811278 1105 60-61 -0.4954016270569852 1105 62-63 -0.4329352119005634 1105 64-65 -0.454801422666705 1105 66-67 -0.4644228989493726 1105 68-69 -0.3991516703929605 1105 70-71 -0.4439387419371741 1105 72-73 -0.37455362295571604 1105 74-75 -0.3721074271381113 1105 76-77 -0.35228657045990985 1105 78-79 -0.3407097474107559 1105 80-81 -0.3253823782389631 1105 82-83 -0.2927480663411579 1105 84-85 -0.30312876951393974 1105 86-87 -0.3145573424467294 1105 88-89 -0.3538771497898381 1105 90-91 -0.3799875459435782 1105 92-93 -0.3908906385517774 1105 94-95 -0.4038353219674278 1105 96-97 -0.4150474989201989 1105 98-99 -0.4347780566170094 1105 100 -0.469827959215241 1106 1 -0.0749642087680904 1106 2 -0.1015990812861709 1106 3 -0.026796823991126928 1106 4 0.04396685289243152 1106 5 0.09841823416059725 1106 6 -0.017271172751797792 1106 7 0.027606461179523478 1106 8 0.031227907515074094 1106 9 0.01871294416984881 1106 10-11 -0.0930017856554457 1106 12-13 -0.007719240653699444 1106 14-15 -0.0577695624030099 1106 16-17 -0.024056981209831463 1106 18-19 -0.06378672986363654 1106 20-21 -0.12200345362526122 1106 22-23 -0.10351636860418978 1106 24-25 -0.07445237716415676 1106 26-27 -0.14726678240295854 1106 28-29 -0.16776268320234067 1106 30-31 -0.15875098437292223 1106 32-33 -0.1807403923637736 1106 34-35 -0.1594920748894637 1106 36-37 -0.21531126846667803 1106 38-39 -0.15811827567426917 1106 40-41 -0.24443606846935495 1106 42-43 -0.23432479671024709 1106 44-45 -0.24360127038277568 1106 46-47 -0.27774097163872113 1106 48-49 -0.22405744379157255 1106 50-51 -0.2742507915101129 1106 52-53 -0.3411185250619013 1106 54-55 -0.32034767094510386 1106 56-57 -0.31167529149892914 1106 58-59 -0.3272158559160232 1106 60-61 -0.34773402521951624 1106 62-63 -0.31312127684834934 1106 64-65 -0.20917050851157626 1106 66-67 -0.08442892124209322 1106 68-69 -0.056277289855238166 1106 70-71 -0.21199548731905793 1106 72-73 -0.2447850726452785 1106 74-75 -0.14232545360133741 1106 76-77 -0.1647500252177565 1106 78-79 -0.11866439709868715 1106 80-81 -0.15496894504689962 1106 82-83 -0.12034192323939763 1106 84-85 -0.09891885930864319 1106 86-87 -0.17067077945947418 1106 88-89 -0.16642357359683402 1106 90-91 -0.20639216164488872 1106 92-93 -0.19376487599557635 1106 94-95 -0.15665396342941662 1106 96-97 -0.1919728711568709 1106 98-99 -0.17409440690569156 1106 100 -0.21518027605103285 1107 1 -0.38447116789769 1107 2 -0.493698766404723 1107 3 -0.3699356178846358 1107 4 -0.34600811349763205 1107 5 -0.2744989659264192 1107 6 -0.3809282907485354 1107 7 -0.35451811490731444 1107 8 -0.2832509690392726 1107 9 -0.30133787191869743 1107 10-11 -0.29770377158385486 1107 12-13 -0.3083824351848179 1107 14-15 -0.3561640414578662 1107 16-17 -0.3880281069734153 1107 18-19 -0.4251870964526745 1107 20-21 -0.34458516054658617 1107 22-23 -0.3930962176179946 1107 24-25 -0.31708850187575166 1107 26-27 -0.3707195484028958 1107 28-29 -0.42122939242376845 1107 30-31 -0.4554176807565966 1107 32-33 -0.4958726033450418 1107 34-35 -0.4909549720127373 1107 36-37 -0.5097454803537289 1107 38-39 -0.5647744165756876 1107 40-41 -0.4796226794106815 1107 42-43 -0.5398901568982453 1107 44-45 -0.5302766679072235 1107 46-47 -0.4936473166960056 1107 48-49 -0.49378066549592603 1107 50-51 -0.5784509106158922 1107 52-53 -0.5605702997023485 1107 54-55 -0.5366054850331068 1107 56-57 -0.4379831014859832 1107 58-59 -0.5838576256710013 1107 60-61 -0.6507145938604566 1107 62-63 -0.6002388100036171 1107 64-65 -0.41660518755003295 1107 66-67 -0.4755872427777774 1107 68-69 -0.5765280717098022 1107 70-71 -0.5345550312936922 1107 72-73 -0.5389981721413974 1107 74-75 -0.4966334811169091 1107 76-77 -0.4949073411297853 1107 78-79 -0.4998415473324407 1107 80-81 -0.523836905235612 1107 82-83 -0.4504225692634414 1107 84-85 -0.36692715392071307 1107 86-87 -0.5002423017668427 1107 88-89 -0.5269764348100701 1107 90-91 -0.46403581070363487 1107 92-93 -0.4390477165614364 1107 94-95 -0.4627665504854015 1107 96-97 -0.5091742954337661 1107 98-99 -0.4978368067801675 1107 100 -0.5193145660343816 1108 1 -0.194596582462502 1108 2 -0.1648077098566887 1108 3 -0.074463223023848 1108 4 -0.19479925644236573 1108 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-0.11805471808983725 2215 14-15 -0.030127174408129065 2215 16-17 -0.031224958924020996 2215 18-19 5.741515026471689E-4 2215 20-21 -0.060931649698581225 2215 22-23 5.711341019321026E-4 2215 24-25 0.012307772473086231 2215 26-27 -0.06364689281291902 2215 28-29 -0.06450545490541515 2215 30-31 -0.08543538628755698 2215 32-33 -0.11237871190301263 2215 34-35 -0.06873360194617106 2215 36-37 -0.09414109561343764 2215 38-39 -0.05814808416707962 2215 40-41 -0.11458816710668174 2215 42-43 -0.02079817202134393 2215 44-45 0.027547169549407613 2215 46-47 0.06776987474542295 2215 48-49 0.11759763968630921 2215 50-51 0.15859463244359517 2215 52-53 0.25342530836729793 2215 54-55 0.2461358764053827 2215 56-57 0.23259956327132159 2215 58-59 0.21134507419778004 2215 60-61 0.2878452963712448 2215 62-63 0.2608089619755063 2215 64-65 0.18709946262342925 2215 66-67 0.27897431315193444 2215 68-69 0.17580788999112684 2215 70-71 0.21087169896508584 2215 72-73 0.14959527171919262 2215 74-75 0.15384663536211463 2215 76-77 0.18605603748097366 2215 78-79 0.24458022178264116 2215 80-81 0.2803422591591911 2215 82-83 0.2723944675916279 2215 84-85 0.2550547271196919 2215 86-87 0.23374911209919347 2215 88-89 0.23080701845751506 2215 90-91 0.31772626410897686 2215 92-93 0.3152141934788908 2215 94-95 0.3653108864966903 2215 96-97 0.2803601180068611 2215 98-99 0.2865798394147916 2215 100 0.3340395985102198 2216 1 0.3142997415316202 2216 2 0.25643605433836214 2216 3 0.23760887645033435 2216 4 0.22085436701792105 2216 5 0.27253439610043273 2216 6 0.24861932109800478 2216 7 0.1748608256040498 2216 8 0.18564219802677684 2216 9 0.2174151234515591 2216 10-11 0.20756389237632078 2216 12-13 0.1416355951992898 2216 14-15 0.2806601099508299 2216 16-17 0.22297653439643028 2216 18-19 0.28876900247014703 2216 20-21 0.3353261997586472 2216 22-23 0.31173708857282634 2216 24-25 0.34390392536291614 2216 26-27 0.3251662087569116 2216 28-29 0.29660486763253857 2216 30-31 0.2773842990958997 2216 32-33 0.2736067838545537 2216 34-35 0.25925080701617986 2216 36-37 0.27880464411408923 2216 38-39 0.3370399053655433 2216 40-41 0.35150381245820483 2216 42-43 0.3691705182523961 2216 44-45 0.4558457525846009 2216 46-47 0.472970706708594 2216 48-49 0.4944383978547009 2216 50-51 0.5398423099596812 2216 52-53 0.5082871555706632 2216 54-55 0.5425325462815991 2216 56-57 0.5103229730085843 2216 58-59 0.5939025934854101 2216 60-61 0.6094172024310964 2216 62-63 0.5035172679806976 2216 64-65 0.49887430434210955 2216 66-67 0.4395382719606147 2216 68-69 0.4240284855337606 2216 70-71 0.40333704231680656 2216 72-73 0.4328792561442043 2216 74-75 0.4765511500163271 2216 76-77 0.4464719250917426 2216 78-79 0.4103471319128431 2216 80-81 0.36336873420323457 2216 82-83 0.40241587849497407 2216 84-85 0.43028168445725257 2216 86-87 0.41456872817522594 2216 88-89 0.4134605402731033 2216 90-91 0.43401964145186867 2216 92-93 0.4099073935983917 2216 94-95 0.3874640057810268 2216 96-97 0.3535289826063881 2216 98-99 0.35457118009836464 2216 100 0.35275113435879746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93566.0 3 19306.0 4 13411.0 5 11428.0 6 7317.0 7 6315.0 8 7267.0 9 7549.0 10 7887.0 11 8600.0 12 9834.0 13 10992.0 14 11649.0 15 11523.0 16 11325.0 17 10939.0 18 10647.0 19 10517.0 20 10344.0 21 11153.0 22 13288.0 23 20543.0 24 32217.0 25 42993.0 26 47303.0 27 37155.0 28 34145.0 29 42027.0 30 49750.0 31 56709.0 32 71438.0 33 95654.0 34 135430.0 35 209649.0 36 349505.0 37 626464.0 38 939385.0 39 466807.0 40 1719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.221694394043105 21.753423348338956 14.345133104056016 21.67974915356193 2 14.645812047014248 22.95361116693768 40.18233972192279 22.218237064125283 3 16.85556875561885 29.818436971322004 30.72360960322409 22.602384669835057 4 11.511766296541369 19.720486156984023 39.434816364369254 29.33293118210536 5 11.754813477061063 41.24024204013611 34.397075591999766 12.60786889080306 6 26.759741113119233 40.93146147305377 19.21668971203215 13.092107701794841 7 24.674076314372822 36.02698992118298 23.21205672623646 16.086877038207735 8 24.175951610190456 37.607538135868054 20.88346444709695 17.333045806844535 9 25.27632259452077 19.016042798766172 21.21867768344434 34.48895692326871 10-11 22.150591742395353 29.951058329575215 29.01938628173037 18.878963646299063 12-13 23.04765247669325 28.21703358418511 28.495844097495226 20.239469841626416 14-15 21.0903590327794 29.467720352519667 26.15003356240382 23.29188705229712 16-17 20.04874480717582 32.42894307162482 26.860298112683058 20.6620140085163 18-19 20.229056505546772 31.278555545353175 28.641708653198965 19.850679295901084 20-21 22.26982318472482 30.084922157613896 27.778482127876746 19.866772529784534 22-23 21.74127854245419 30.142345127052845 27.92026112003738 20.196115210455584 24-25 21.116644708245023 30.32674243726125 27.898539736880828 20.6580731176129 26-27 20.758285601000566 30.35379023457968 28.001899933112025 20.886024231307733 28-29 20.8434567698781 30.224911228488764 28.254577501520185 20.677054500112952 30-31 21.033744503734006 30.54351324074277 27.868209805299216 20.554532450224006 32-33 20.76798507010044 30.39243216285856 28.217027184278454 20.622555582762548 34-35 21.09666205207839 30.10464257533339 28.093955076561915 20.704740296026305 36-37 21.163391361687356 30.19304996534475 27.784004551801456 20.859554121166436 38-39 20.8958924875097 30.259721878604186 28.095166953433665 20.74921868045245 40-41 20.86628180604058 29.98411172228032 28.265535298485084 20.884071173194023 42-43 20.794278490027292 30.155225275468982 28.14786389734621 20.90263233715752 44-45 21.005913129833143 30.133974679519994 28.088960524041283 20.771151666605583 46-47 20.997548721580657 30.17633534859179 28.026615564901867 20.799500364925684 48-49 20.964296060000695 29.991247815990985 28.05534840165574 20.989107722352585 50-51 20.85088368396591 29.967915820098316 28.02680187422159 21.15439862171418 52-53 20.892989329879594 29.992316704540222 28.0950397812732 21.019654184306983 54-55 20.98114535386354 30.229019381198484 27.962639776831665 20.827195488106305 56-57 20.97727112218999 29.969710461326816 27.84845992024162 21.204558496241575 58-59 20.991360408798457 29.762941659415404 28.09076815259847 21.15492977918767 60-61 21.14175407479464 30.033513274587282 27.784411412935274 21.040321237682804 62-63 20.891967332270692 30.02357457764497 27.95491978030757 21.129538309776773 64-65 20.985703225893413 29.85176430217966 28.013539435121153 21.148993036805777 66-67 20.79538689640454 29.883563220972942 27.97843960956542 21.342610273057094 68-69 21.14882763134821 30.10946637686047 27.709734037153734 21.03197195463759 70-71 21.05934102899865 29.774196931477352 27.933142310561244 21.233319728962748 72-73 20.929569120312458 29.8821682832903 28.050912062132262 21.137350534264982 74-75 20.897417987531142 30.055296245934564 27.935857197111424 21.11142856942287 76-77 20.96487879272491 29.92388189021374 27.81354299452376 21.29769632253759 78-79 20.996261806898634 29.953183080200912 27.967974390744725 21.08258072215573 80-81 20.902029564413745 29.95723175630905 27.921952553773426 21.218786125503783 82-83 20.83595910646204 29.88669016663409 28.170080452565337 21.10727027433853 84-85 20.797442602914845 29.914657010431338 27.949527185964534 21.338373200689283 86-87 21.0284052766397 29.857393011761925 27.876287177502686 21.237914534095697 88-89 20.888328725606534 29.786570206998775 28.00811263213603 21.316988435258665 90-91 20.867351709926275 29.814237408461747 27.912128893947475 21.406281987664503 92-93 20.917963838724173 29.849317955358533 27.867943768470564 21.364774437446727 94-95 20.80986022770334 29.71051088288642 28.10187921032928 21.377749679080964 96-97 20.87289583242952 29.921737749647566 27.839770667724707 21.365595750198207 98-99 20.763065629681844 29.869445501430093 27.864792268387923 21.502696600500144 100 20.75962705601967 29.924350721021657 27.807288685579735 21.508733537378934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18086.0 1 13580.5 2 7996.0 3 5865.5 4 4059.5 5 2946.0 6 2379.0 7 2186.0 8 2339.0 9 2365.0 10 2039.0 11 1754.5 12 1690.5 13 1671.5 14 1706.5 15 1883.0 16 2111.5 17 2462.5 18 3106.0 19 3985.0 20 5365.0 21 7385.5 22 11050.5 23 16828.0 24 22929.0 25 29066.0 26 37187.5 27 47446.0 28 60737.0 29 73553.5 30 80635.5 31 87092.0 32 95003.0 33 102378.0 34 109159.5 35 115185.5 36 117905.0 37 119076.0 38 119526.5 39 118582.5 40 119345.0 41 118119.0 42 114864.5 43 113470.5 44 112723.5 45 110269.5 46 108452.5 47 105972.0 48 101969.0 49 99514.5 50 98848.5 51 95369.0 52 87886.0 53 81540.0 54 78216.0 55 76554.5 56 70638.5 57 62649.0 58 57027.0 59 52071.0 60 46202.5 61 38996.5 62 32898.0 63 27336.5 64 21735.0 65 17621.5 66 14132.0 67 10765.0 68 8275.5 69 6434.5 70 5000.0 71 3929.5 72 3028.0 73 2323.5 74 1933.5 75 1548.0 76 1116.0 77 891.5 78 669.5 79 474.0 80 344.5 81 234.0 82 191.5 83 154.0 84 118.5 85 92.5 86 77.0 87 71.5 88 64.0 89 48.5 90 35.0 91 28.5 92 25.5 93 23.5 94 20.0 95 24.0 96 23.0 97 19.0 98 20.0 99 21.0 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1738337425464749 2 0.17130831287267625 3 0.18317783233953 4 0.1744230094703613 5 0.1867134338828481 6 0.0745843563661873 7 0.07623991581901088 8 0.06563311118905647 9 0.06038582953349702 10-11 0.07217116801122414 12-13 0.07155384075762891 14-15 0.06940722553490003 16-17 0.06580147316730972 18-19 0.06984216064538758 20-21 0.07148368993335671 22-23 0.06947737635917221 24-25 0.06942125569975446 26-27 0.07217116801122414 28-29 0.07016485443703964 30-31 0.07565064889512452 32-33 0.07222728867064189 34-35 0.07239565064889512 36-37 0.08311469659768503 38-39 0.07820413889863206 40-41 0.07391090845317431 42-43 0.07666082076464399 44-45 0.08739389687828833 46-47 0.07719396702911259 48-49 0.08206243423360224 50-51 0.0787092248333918 52-53 0.0779515959312522 54-55 0.07998596983514557 56-57 0.07350403367239565 58-59 0.08068747807786741 60-61 0.07781129428270782 62-63 0.07010873377762189 64-65 0.07287267625394599 66-67 0.07267625394598386 68-69 0.07069800070150824 70-71 0.06719045948789898 72-73 0.0649596632760435 74-75 0.06951946685373553 76-77 0.07406524026657313 78-79 0.07120308663626797 80-81 0.07894773763591723 82-83 0.08120659417748158 84-85 0.08255349000350753 86-87 0.07883549631708173 88-89 0.08091196071553841 90-91 0.07876534549280954 92-93 0.0704173974044195 94-95 0.06996843212907751 96-97 0.06887407927043143 98-99 0.07814801823921431 100 0.08721150473518065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3563750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.663643741647675 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6147914597972 44.35839187796526 2 14.467118196291008 16.97387735671048 3 4.5732015727501105 8.04842003467666 4 1.9392565425162045 4.550554197353201 5 0.9677069621925728 2.8384608238188602 6 0.5813946639838041 2.0464037664744503 7 0.35887737583325435 1.4737138165973602 8 0.25239738768431746 1.1845240345948265 9 0.19083435721356606 1.0075534870718676 >10 0.9527831795007096 10.090489965117234 >50 0.0668148432298348 2.709366833510453 >100 0.03310111330992377 3.3263744365885133 >500 0.001387445150365866 0.5481369783596699 >1k 2.870576118671819E-4 0.27589280171356906 >5k 0.0 0.0 >10k+ 4.78429353111969E-5 0.5678395894474999 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20232 0.5677165906699404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.403016485443704E-5 0.0 20-21 0.0 0.0 0.0 2.806032970887408E-5 0.0 22-23 0.0 0.0 0.0 1.5433181339880744E-4 0.0 24-25 0.0 0.0 0.0 6.173272535952296E-4 0.0 26-27 0.0 0.0 0.0 0.0011645036829182743 0.0 28-29 0.0 0.0 0.0 0.002090494563311119 0.0 30-31 0.0 0.0 0.0 0.004615924237109786 0.0 32-33 0.0 1.403016485443704E-5 0.0 0.00942827078218169 0.0 34-35 0.0 2.806032970887408E-5 0.0 0.015924237109786042 0.0 36-37 0.0 2.806032970887408E-5 0.0 0.027751666082076465 0.0 38-39 0.0 2.806032970887408E-5 0.0 0.046902841108383025 0.0 40-41 0.0 2.806032970887408E-5 0.0 0.06620834794808839 0.0 42-43 0.0 2.806032970887408E-5 0.0 0.08561206594177481 1.403016485443704E-5 44-45 0.0 2.806032970887408E-5 0.0 0.11008067344791302 2.806032970887408E-5 46-47 0.0 2.806032970887408E-5 0.0 0.14153630305156084 2.806032970887408E-5 48-49 0.0 2.806032970887408E-5 0.0 0.17633111189056472 2.806032970887408E-5 50-51 0.0 2.806032970887408E-5 0.0 0.21475973342686777 2.806032970887408E-5 52-53 0.0 2.806032970887408E-5 0.0 0.25227639424763243 2.806032970887408E-5 54-55 0.0 2.806032970887408E-5 0.0 0.2952928796913364 2.806032970887408E-5 56-57 0.0 2.806032970887408E-5 0.0 0.3466292528937215 2.806032970887408E-5 58-59 0.0 2.806032970887408E-5 0.0 0.4059768502279902 2.806032970887408E-5 60-61 0.0 2.806032970887408E-5 0.0 0.47240968081374957 2.806032970887408E-5 62-63 0.0 2.806032970887408E-5 0.0 0.5424903542616626 2.806032970887408E-5 64-65 0.0 2.806032970887408E-5 0.0 0.6143668888109435 2.806032970887408E-5 66-67 0.0 2.806032970887408E-5 0.0 0.6977341283760083 2.806032970887408E-5 68-69 0.0 2.806032970887408E-5 0.0 0.7973623290073658 2.806032970887408E-5 70-71 0.0 2.806032970887408E-5 0.0 0.9097579796562609 2.806032970887408E-5 72-73 0.0 2.806032970887408E-5 0.0 1.0336443353209401 2.806032970887408E-5 74-75 0.0 2.806032970887408E-5 0.0 1.155819010873378 2.806032970887408E-5 76-77 0.0 2.806032970887408E-5 0.0 1.2905787443002454 2.806032970887408E-5 78-79 0.0 2.806032970887408E-5 0.0 1.4506208347948089 2.806032970887408E-5 80-81 0.0 2.806032970887408E-5 0.0 1.6343037530690987 2.806032970887408E-5 82-83 0.0 2.806032970887408E-5 0.0 1.8332795510347246 2.806032970887408E-5 84-85 0.0 2.806032970887408E-5 0.0 2.029982462293932 2.806032970887408E-5 86-87 0.0 2.806032970887408E-5 0.0 2.2401403016485446 2.806032970887408E-5 88 0.0 2.806032970887408E-5 0.0 2.415601543318134 2.806032970887408E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5425 0.0 51.42839 1 GTATCAA 9770 0.0 39.29557 1 ATCAACG 11520 0.0 32.180836 3 TCAACGC 11625 0.0 31.969316 4 TATCAAC 12455 0.0 30.444927 2 CAACGCA 12395 0.0 29.983322 5 AACGCAG 13240 0.0 28.288235 6 GTACATG 22055 0.0 27.305552 1 CATGGGT 9580 0.0 27.008245 4 TACATGG 22020 0.0 26.599632 2 CGCAGAG 14485 0.0 25.46752 8 ACGCAGA 14880 0.0 24.918144 7 ACATGGG 22830 0.0 24.893423 3 TACCTGG 2525 0.0 24.780716 2 ATGGGTG 5475 0.0 24.230598 5 GTGGTAT 2645 0.0 23.657782 1 GCAGAGT 16050 0.0 22.282164 9 GTACCTG 2985 0.0 21.908796 1 TGGTATC 2780 0.0 20.81536 2 CAGAGTA 15680 0.0 19.016617 10-11 >>END_MODULE