FastQCFastQC Report
Wed 25 May 2016
SRR2431424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3563750
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323840.9087057172921782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252340.7080743598737285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160770.4511259207295686No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56610.15884952648193618No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50210.14089091546825677No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49830.13982462293931952No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.11636618730270079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111200.065.45741
GTATCAA228750.046.558571
ATCAACG297650.035.5086563
TATCAAC299550.035.3643882
TCAACGC301300.035.1248174
GTGGTAT35600.034.869561
TGGTATC35000.034.7941322
CAACGCA310150.034.1377035
AACGCAG321200.033.0687876
CGCAGAG360600.029.3643788
ACGCAGA361900.029.2978577
CATGGGT91050.027.625594
TATAACG3750.027.5845362
ATAACGC4650.027.2994963
GCAGAGT386450.027.2055849
GTACATG212000.025.5067181
TACATGG212000.025.0620672
ACATGGG213550.024.305993
TACCTGG26400.024.2219562
GAGTACT265400.023.24254812-13
Produced by FastQC (version 0.11.5)