##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431424_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3563750
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63629687828832	34.0	31.0	34.0	31.0	34.0
2	32.72946418800421	34.0	31.0	34.0	31.0	34.0
3	32.82751764293231	34.0	31.0	34.0	31.0	34.0
4	36.30624763240968	37.0	37.0	37.0	35.0	37.0
5	36.219971939670295	37.0	37.0	37.0	35.0	37.0
6	36.26632816555595	37.0	37.0	37.0	35.0	37.0
7	36.2197017186952	37.0	37.0	37.0	35.0	37.0
8	36.23308733777622	37.0	37.0	37.0	35.0	37.0
9	38.01985773412838	39.0	39.0	39.0	35.0	39.0
10-11	37.99450775166608	39.0	39.0	39.0	35.0	39.0
12-13	37.97705071904595	39.0	38.5	39.0	35.0	39.0
14-15	39.41292360575237	41.0	39.0	41.0	36.0	41.0
16-17	39.44668621536303	41.0	39.0	41.0	36.0	41.0
18-19	39.537052262364085	41.0	39.5	41.0	36.5	41.0
20-21	39.539623991581905	41.0	40.0	41.0	36.5	41.0
22-23	39.475814240617325	41.0	39.0	41.0	36.0	41.0
24-25	39.42877264117853	41.0	39.0	41.0	36.0	41.0
26-27	39.30812360575237	41.0	39.0	41.0	36.0	41.0
28-29	39.22499684321291	41.0	39.0	41.0	35.0	41.0
30-31	39.02693370747106	41.0	39.0	41.0	35.0	41.0
32-33	38.77931013679411	41.0	38.0	41.0	35.0	41.0
34-35	38.6376676253946	41.0	38.0	41.0	35.0	41.0
36-37	38.49119621185549	41.0	38.0	41.0	35.0	41.0
38-39	38.34255601543318	40.0	38.0	41.0	35.0	41.0
40-41	38.19529386180288	40.0	38.0	41.0	34.0	41.0
42-43	38.006200631357416	40.0	38.0	41.0	34.0	41.0
44-45	37.73334857944581	40.0	38.0	41.0	33.0	41.0
46-47	37.325669589617675	40.0	37.5	41.0	32.5	41.0
48-49	36.95530031567871	40.0	37.0	41.0	31.0	41.0
50-51	36.77598807435987	40.0	37.0	41.0	31.0	41.0
52-53	36.70989000350754	40.0	37.0	41.0	31.0	41.0
54-55	36.56719424763241	40.0	36.0	41.0	31.0	41.0
56-57	36.41163535601544	40.0	36.0	41.0	31.0	41.0
58-59	36.22645640126271	40.0	35.0	41.0	30.0	41.0
60-61	36.06862307962119	39.0	35.0	41.0	30.0	41.0
62-63	35.90289877236057	39.0	35.0	41.0	30.0	41.0
64-65	35.66586390740091	39.0	35.0	41.0	29.5	41.0
66-67	35.397078919677305	38.5	35.0	41.0	29.0	41.0
68-69	35.10018112942827	37.5	35.0	40.5	29.0	41.0
70-71	34.802104103823226	37.0	35.0	40.0	29.0	41.0
72-73	34.4811427569274	36.5	35.0	39.5	28.0	41.0
74-75	34.1458508593476	36.0	35.0	39.0	28.0	41.0
76-77	33.818388214661525	36.0	34.5	39.0	28.0	41.0
78-79	33.50462644686075	35.0	34.0	37.5	27.5	39.5
80-81	33.24379544019642	35.0	34.0	37.0	27.5	39.0
82-83	33.046908032269386	35.0	34.0	37.0	28.0	39.0
84-85	32.78976106629253	35.0	34.0	36.0	27.5	38.0
86-87	32.55041220624342	35.0	34.0	36.0	27.0	37.0
88-89	32.32539852683269	35.0	34.0	36.0	27.0	37.0
90-91	32.14379628200631	35.0	34.0	35.0	26.5	36.5
92-93	31.98477207997194	35.0	34.0	35.0	26.0	36.0
94-95	31.84894535250789	35.0	34.0	35.0	26.0	36.0
96-97	31.70872900736584	35.0	34.0	35.0	25.5	36.0
98-99	31.595205892669238	35.0	34.0	35.0	25.0	36.0
100	31.494473518063838	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.01314581903717027
1101	2	0.014873898547939746
1101	3	0.011647323792189468
1101	4	0.053144742055721395
1101	5	0.00489930026962071
1101	6	-1.8720492514745501E-4
1101	7	0.02662137357068417
1101	8	0.06598284301168889
1101	9	0.024489384472538234
1101	10-11	0.03917865560960365
1101	12-13	0.0671637498950588
1101	14-15	0.04476855364076471
1101	16-17	0.022698766466405118
1101	18-19	0.027664115751314
1101	20-21	0.053950702361035496
1101	22-23	0.05899176456339461
1101	24-25	0.12116276408469417
1101	26-27	0.11473386645102579
1101	28-29	0.03832470405362898
1101	30-31	-0.005801117728402971
1101	32-33	0.00916935703887134
1101	34-35	0.03848107059300787
1101	36-37	0.029392999966660227
1101	38-39	-0.02761308118770245
1101	40-41	-0.0510283334253856
1101	42-43	-0.06752083489401883
1101	44-45	-0.09671614435355735
1101	46-47	-0.0053537306638702375
1101	48-49	0.009671928723484768
1101	50-51	-0.05405757816377843
1101	52-53	0.0012334617544453863
1101	54-55	-0.04946143061773256
1101	56-57	-0.08425273387668142
1101	58-59	-0.10329654137254352
1101	60-61	-0.0912186674989357
1101	62-63	-0.08595444060164681
1101	64-65	-0.06849240734774042
1101	66-67	-0.12206564638790951
1101	68-69	-0.17907318379704407
1101	70-71	-0.12560813628302014
1101	72-73	-0.19044949867065242
1101	74-75	-0.1507462440001106
1101	76-77	-0.17063957669675744
1101	78-79	-0.11419099590639803
1101	80-81	-0.047999812476831494
1101	82-83	-0.14251376718780762
1101	84-85	-0.10907413441146474
1101	86-87	-0.10734572424121325
1101	88-89	-0.06997638324794764
1101	90-91	-0.13191617375907327
1101	92-93	-0.1543057823653058
1101	94-95	-0.11140180560133572
1101	96-97	-0.08206671811327126
1101	98-99	-0.1318906182882067
1101	100	-0.0728097460620809
1102	1	-0.016217203964565385
1102	2	-8.678264350834297E-4
1102	3	0.010112828560842502
1102	4	0.040294843924797874
1102	5	2.487554915191481E-4
1102	6	-0.0443240121416153
1102	7	0.01265128469360377
1102	8	0.04400440529946792
1102	9	0.04298729541829971
1102	10-11	0.05714369163287358
1102	12-13	0.012854798703635595
1102	14-15	-0.042297672331415015
1102	16-17	0.0016098377157049981
1102	18-19	-0.021741002062491077
1102	20-21	0.04340439253141426
1102	22-23	0.04247541014759548
1102	24-25	0.07472375265764697
1102	26-27	0.08045639870956478
1102	28-29	0.010989835302332551
1102	30-31	0.008853173321881513
1102	32-33	0.030685969818271985
1102	34-35	-0.01770240078030838
1102	36-37	-0.01500862993855634
1102	38-39	0.019862614179473326
1102	40-41	0.07868818634312191
1102	42-43	0.1072990487685459
1102	44-45	0.060774923647734624
1102	46-47	0.0381828425489914
1102	48-49	0.00683915640825461
1102	50-51	0.03127576624227402
1102	52-53	0.0798608866813666
1102	54-55	-0.011074597564913802
1102	56-57	0.04315419986411939
1102	58-59	0.06141855739616631
1102	60-61	0.005089132129022289
1102	62-63	0.048899823874101855
1102	64-65	0.01931799806165202
1102	66-67	0.10132198021025829
1102	68-69	0.12871629416085284
1102	70-71	0.10752764116995905
1102	72-73	0.12019766523521724
1102	74-75	0.09488648845330516
1102	76-77	0.059668964803819335
1102	78-79	0.07247532012395652
1102	80-81	0.05326856824776627
1102	82-83	0.057393683280203334
1102	84-85	0.046075052112293235
1102	86-87	0.0569006291411327
1102	88-89	0.03845328279864901
1102	90-91	0.06448260676473438
1102	92-93	0.0548109463086206
1102	94-95	0.04203018571764616
1102	96-97	0.07239595941943122
1102	98-99	0.058536733558721465
1102	100	0.07930274544883176
1103	1	-0.1016412281013217
1103	2	-0.11406094719295368
1103	3	-0.04527521610454244
1103	4	-0.016653417191434983
1103	5	-0.02899236664642757
1103	6	-0.06497758478938209
1103	7	-0.08426018755362463
1103	8	-0.07626726226012437
1103	9	-0.017818170642883047
1103	10-11	-0.042809760410086994
1103	12-13	-0.04741711790137515
1103	14-15	-0.10083908387837681
1103	16-17	-0.04477893525570664
1103	18-19	-0.11873453401866385
1103	20-21	-0.11262038460330359
1103	22-23	-0.08445288861094724
1103	24-25	-0.07041529061916663
1103	26-27	-0.08438608613207066
1103	28-29	-0.08411841138141796
1103	30-31	-0.08683636285226726
1103	32-33	-0.08381784741032305
1103	34-35	-0.1311554816848357
1103	36-37	-0.14302018139437678
1103	38-39	-0.12003438351716511
1103	40-41	-0.1511776108209162
1103	42-43	-0.1548094184115243
1103	44-45	-0.1480927943550867
1103	46-47	-0.19044254893826462
1103	48-49	-0.22975764390469777
1103	50-51	-0.27078898359462755
1103	52-53	-0.19978918627072773
1103	54-55	-0.1723792516468876
1103	56-57	-0.18633502998414997
1103	58-59	-0.23374192049163156
1103	60-61	-0.23322832152118878
1103	62-63	-0.25317244288270757
1103	64-65	-0.2523195697728511
1103	66-67	-0.25821879065215825
1103	68-69	-0.22082947887007975
1103	70-71	-0.23935374441553847
1103	72-73	-0.2402187508674487
1103	74-75	-0.2093520748947384
1103	76-77	-0.18589246483234234
1103	78-79	-0.21320091186125012
1103	80-81	-0.203760700967905
1103	82-83	-0.22520235789976795
1103	84-85	-0.25064533851242743
1103	86-87	-0.19232539385851766
1103	88-89	-0.22706832158843326
1103	90-91	-0.2569403568029571
1103	92-93	-0.262694525097924
1103	94-95	-0.26136018053706067
1103	96-97	-0.2515414518201702
1103	98-99	-0.3037093502019381
1103	100	-0.22906693484973317
1104	1	-0.16891110300711887
1104	2	-0.13980340756642562
1104	3	-0.1440805074371454
1104	4	-0.06940736299088712
1104	5	-0.047853227666422526
1104	6	-0.07007999300722645
1104	7	-0.10997379880490143
1104	8	-0.12555083055871563
1104	9	-0.08842486506674163
1104	10-11	-0.1478308230719847
1104	12-13	-0.11668092679343545
1104	14-15	-0.16410003319461453
1104	16-17	-0.14137530685078303
1104	18-19	-0.12717503231176153
1104	20-21	-0.09831441507343186
1104	22-23	-0.1413934885299355
1104	24-25	-0.13534593893629676
1104	26-27	-0.17729139565231833
1104	28-29	-0.20877298331519256
1104	30-31	-0.13780584844065658
1104	32-33	-0.14754746416954134
1104	34-35	-0.18348235210478947
1104	36-37	-0.22595391514754937
1104	38-39	-0.2635284038452568
1104	40-41	-0.3359590598487969
1104	42-43	-0.33031798602159057
1104	44-45	-0.30988543545971226
1104	46-47	-0.38386668301524196
1104	48-49	-0.3096995704722403
1104	50-51	-0.41482235545200297
1104	52-53	-0.3481588390420214
1104	54-55	-0.3793591846533957
1104	56-57	-0.4138905530533066
1104	58-59	-0.36249411411797183
1104	60-61	-0.3559402675577914
1104	62-63	-0.3894254082791164
1104	64-65	-0.3788838639093868
1104	66-67	-0.45272265544424783
1104	68-69	-0.4406959448036645
1104	70-71	-0.43542874660257525
1104	72-73	-0.4199518837816001
1104	74-75	-0.4015637984530329
1104	76-77	-0.4315915820155709
1104	78-79	-0.46474008913735076
1104	80-81	-0.42395059844627525
1104	82-83	-0.41797693829544613
1104	84-85	-0.4405594835995146
1104	86-87	-0.4083474526746258
1104	88-89	-0.3721047609819408
1104	90-91	-0.4213643965971059
1104	92-93	-0.447682944155936
1104	94-95	-0.48922095497231766
1104	96-97	-0.4980856763847292
1104	98-99	-0.4773876428032615
1104	100	-0.38672488388716175
1105	1	-0.14957585647666605
1105	2	-0.1654950604447194
1105	3	-0.14638583115422676
1105	4	-0.0456335541627908
1105	5	-0.0697475946793702
1105	6	-0.04489429794015365
1105	7	-0.046724908752175054
1105	8	-0.05286789056390262
1105	9	-0.04627988053246668
1105	10-11	-0.1327032421481107
1105	12-13	-0.13547041919841263
1105	14-15	-0.15213645442453583
1105	16-17	-0.11775694714741292
1105	18-19	-0.20873786647254633
1105	20-21	-0.10486394785986874
1105	22-23	-0.17327156303220903
1105	24-25	-0.1506800852222625
1105	26-27	-0.11032421755562183
1105	28-29	-0.13752285933507835
1105	30-31	-0.13294382064502486
1105	32-33	-0.2546168360784975
1105	34-35	-0.17085102798530727
1105	36-37	-0.11900066074214521
1105	38-39	-0.14328678649925308
1105	40-41	-0.09463088304713807
1105	42-43	-0.08158444191835201
1105	44-45	-0.038723406132447735
1105	46-47	-0.06948145112447435
1105	48-49	-0.04490140116961783
1105	50-51	-0.08217855227903215
1105	52-53	-0.10337503730550424
1105	54-55	-0.06026019923078252
1105	56-57	-0.06968602282378811
1105	58-59	-0.13370967608654638
1105	60-61	-0.16551484811839856
1105	62-63	-0.20927898089146169
1105	64-65	-0.2503212852182628
1105	66-67	-0.2496186061311647
1105	68-69	-0.15715913910528911
1105	70-71	-0.17894791403914923
1105	72-73	-0.1147600837628957
1105	74-75	-0.07689039217552818
1105	76-77	-0.12321217343527024
1105	78-79	-0.05817443707780967
1105	80-81	-0.08743521428042555
1105	82-83	-0.06966818313605216
1105	84-85	-0.05043905894886791
1105	86-87	-0.11992467819381147
1105	88-89	-0.04292582084199381
1105	90-91	-0.0328696355839142
1105	92-93	-0.06920060866360345
1105	94-95	-0.06628347842072912
1105	96-97	-0.0686424411336013
1105	98-99	-0.07813614809013103
1105	100	-0.10953829482497923
1106	1	-0.13963380733400044
1106	2	-0.08369909087011962
1106	3	-0.12492464904739364
1106	4	-0.0688149503265052
1106	5	-0.07331139589881985
1106	6	-0.0839088907595098
1106	7	-0.08453998230898208
1106	8	-0.07416153656930646
1106	9	-0.08651680047730537
1106	10-11	-0.06548171489583865
1106	12-13	-0.10374371942790361
1106	14-15	-0.13007442313882223
1106	16-17	-0.1651730596096499
1106	18-19	-0.12819166246362101
1106	20-21	-0.13645853998481527
1106	22-23	-0.09583638058165178
1106	24-25	-0.05698627409955037
1106	26-27	-0.07507924149588519
1106	28-29	-0.15518954667893325
1106	30-31	-0.18510264298063106
1106	32-33	-0.09677153411328732
1106	34-35	-0.023769221410802288
1106	36-37	-0.0392820044477773
1106	38-39	-0.03117718774477396
1106	40-41	-0.1183287792469585
1106	42-43	-0.18086234788342637
1106	44-45	-0.11452394033037194
1106	46-47	-0.047079626262998886
1106	48-49	0.09572570755894816
1106	50-51	0.02161719909550186
1106	52-53	0.033606953634979675
1106	54-55	0.03959006424361888
1106	56-57	0.12491834501270205
1106	58-59	0.09271551027330815
1106	60-61	0.015684398030892055
1106	62-63	-0.012994784895504097
1106	64-65	-0.02398288132413029
1106	66-67	0.043650288694223605
1106	68-69	-3.4108076916794516E-4
1106	70-71	0.007299351377987762
1106	72-73	0.03082226925604914
1106	74-75	-0.031932081171866855
1106	76-77	9.374346689838831E-4
1106	78-79	0.0250925307010732
1106	80-81	-0.0012134479087748673
1106	82-83	0.03574797973590904
1106	84-85	0.03441298266720594
1106	86-87	0.032760944034471606
1106	88-89	-0.015481606041902296
1106	90-91	-0.037806539156484575
1106	92-93	-0.04176781595409906
1106	94-95	0.013578562336775235
1106	96-97	0.009848857957234003
1106	98-99	0.026065568717275056
1106	100	0.02562189990477748
1107	1	-0.1916102857255737
1107	2	-0.16611939876212745
1107	3	-0.17668748590456573
1107	4	-0.17714226299344205
1107	5	-0.16676382312483184
1107	6	-0.1640331316384689
1107	7	-0.13910972159570179
1107	8	-0.15348773306531172
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2216	32-33	0.061421594949138125
2216	34-35	0.01346968566472384
2216	36-37	0.022224113238976884
2216	38-39	0.08209252193369565
2216	40-41	0.06626018670760203
2216	42-43	0.10594434652191609
2216	44-45	-0.001006284426772197
2216	46-47	0.02455160558709224
2216	48-49	0.07076219962689123
2216	50-51	0.09093925238796174
2216	52-53	0.0524978400505276
2216	54-55	0.04357188573469273
2216	56-57	0.09212959420607092
2216	58-59	0.08550778065497155
2216	60-61	0.05741868683669793
2216	62-63	0.050768658645985454
2216	64-65	0.024189109896568084
2216	66-67	0.044521181057106674
2216	68-69	0.03690746857864724
2216	70-71	0.09032837537495908
2216	72-73	0.10571180260735957
2216	74-75	0.07376840106991978
2216	76-77	0.054581561062803985
2216	78-79	0.10971400072637039
2216	80-81	0.08739858282717705
2216	82-83	0.09853603897896335
2216	84-85	0.11523029953569619
2216	86-87	0.062717124422214
2216	88-89	0.166706506941523
2216	90-91	0.13382348959438417
2216	92-93	0.0868534107713188
2216	94-95	0.07299712807470371
2216	96-97	0.10356574359767379
2216	98-99	0.11077116829648403
2216	100	0.17173827228502248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	230.0
4	64.0
5	115.0
6	305.0
7	700.0
8	1214.0
9	2050.0
10	3172.0
11	4790.0
12	7301.0
13	11132.0
14	12148.0
15	16021.0
16	21466.0
17	33415.0
18	38903.0
19	20110.0
20	12002.0
21	10345.0
22	10388.0
23	10357.0
24	11112.0
25	12649.0
26	15625.0
27	19540.0
28	24468.0
29	30339.0
30	38756.0
31	48294.0
32	61428.0
33	79710.0
34	112589.0
35	187368.0
36	302010.0
37	619217.0
38	1095641.0
39	682375.0
40	6361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19642457260268	20.874965553973485	14.988693845961299	21.939916027462534
2	14.404270293265567	21.983664814979026	40.781586913892596	22.83047797786281
3	16.07344241981055	29.229705950276074	32.303490989885	22.393360640028376
4	10.780273588214662	18.88401262714837	39.98978603998597	30.345927744651004
5	11.014982430993541	39.46860387525935	35.34265949028125	14.173754203465855
6	26.454212556997547	40.53365135040337	18.829519466853736	14.182616625745354
7	23.84743598737285	35.840336723956504	23.63086636267976	16.68136092599088
8	25.267877937565768	34.661381971238164	21.077825324447563	18.99291476674851
9	26.2699344257603	15.693033203535492	22.211958368537385	35.825074002166815
10-11	22.79037530690986	27.67960715538408	29.438877586811646	20.091139950894423
12-13	24.67468256752017	26.197713083128725	29.62655910206945	19.501045247281656
14-15	21.92499473868818	26.83041739740442	28.843689933356714	22.400897930550684
16-17	19.143823219922833	30.269112592072954	29.265085934759732	21.321978253244474
18-19	19.144015755333818	27.494682210051195	33.81665441597892	19.544647618636066
20-21	20.958176078568926	26.419670291125918	33.656850227990184	18.96530340231498
22-23	20.358807435987373	26.50602595580498	33.87685724307261	19.25830936513504
24-25	19.881046600230068	26.735767128348144	33.76248351981067	19.62070275161112
26-27	19.573864250183863	26.819959441565295	33.81501268187669	19.79116362637415
28-29	19.608150976009785	26.72330652165068	34.03466016788519	19.633882334454345
30-31	19.865357893271796	27.008435510519273	33.613230916698846	19.512975679510088
32-33	19.61302169590489	26.937388915047617	33.860250850657486	19.589338538390006
34-35	19.94851173259886	26.782569752515943	33.56457548836898	19.70434302651622
36-37	20.06351513552912	26.892563734358855	33.091385610672006	19.952535519440023
38-39	19.905472464404156	27.02353311080391	33.23320397892822	19.837790445863714
40-41	19.8501351387754	26.88418416137635	33.22692370720231	20.038756992645936
42-43	19.902125089355053	27.06670604485606	32.88706433493535	20.144104530853536
44-45	20.21742024924072	27.15944623960731	32.4961939600872	20.126939551064773
46-47	20.37982488938961	27.445013991591715	31.823635091992347	20.351526027026328
48-49	20.536881546305054	27.578823637031473	31.20057344138066	20.68372137528281
50-51	20.51266905862841	27.704071689823746	30.918614607709344	20.864644643838506
52-53	20.520996186440023	27.877781442788674	30.895981051316873	20.70524131945443
54-55	20.614249848964388	28.135669126356127	30.746585949717275	20.50349507496221
56-57	20.56487011413602	27.829959229017483	30.696316861766597	20.9088537950799
58-59	20.50923273173587	27.57242824520573	31.036673893169354	20.881665129889043
60-61	20.629261085222897	27.749027385572855	30.866500576153648	20.7552109530506
62-63	20.418874097852804	27.63824037803194	31.109963745748825	20.832921778366426
64-65	20.47998020040635	27.592863559726176	31.114895869037586	20.81226037082989
66-67	20.413127955376076	27.626115103563293	31.00342519519881	20.957331745861822
68-69	20.77948316181905	27.802219911295584	30.672317836344114	20.745979090541244
70-71	20.712344434827518	27.603083783200205	30.724916902596277	20.959654879376004
72-73	20.612804616266143	27.77473478749608	30.687442371033175	20.925018225204603
74-75	20.659976398347407	27.924492670397495	30.50096198270718	20.91456894854792
76-77	20.69039442090326	27.850657078213814	30.405788203430618	21.053160297452305
78-79	20.717001065596254	27.862122086885027	30.514537777456642	20.906339070062078
80-81	20.634220681264114	27.890203741699704	30.45503192162814	21.020543655408044
82-83	20.514348377661165	27.865627787647323	30.67696528873116	20.94305854596035
84-85	20.47588521701993	27.907747431648954	30.42350591019235	21.192861441138767
86-87	20.61107216130635	27.876659772621377	30.326947794618714	21.18532027145356
88-89	20.48653742788246	27.789467363187303	30.458663413879933	21.265331795050304
90-91	20.430634643547734	27.807511090246507	30.359972466949536	21.401881799256227
92-93	20.441895544130837	27.840774607288377	30.32015322210766	21.39717662647313
94-95	20.306751762935924	27.668437705060867	30.555035267136653	21.46977526486656
96-97	20.383649676492098	27.815590673409385	30.298259191311615	21.502500458786905
98-99	20.237382703952225	27.779405281924408	30.319378046484324	21.663833967639043
100	20.24890683755815	27.77902334708199	30.297358704036736	21.674711111323123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1728.0
1	1056.5
2	2437.0
3	4307.0
4	2575.0
5	1405.5
6	3437.5
7	8917.0
8	16554.0
9	18612.5
10	11785.5
11	5617.5
12	4616.5
13	4916.5
14	5366.0
15	5872.5
16	6420.5
17	7052.0
18	7757.5
19	8547.0
20	9753.0
21	11637.0
22	14979.5
23	20408.5
24	26142.5
25	31623.0
26	38880.5
27	48503.0
28	61565.0
29	73523.5
30	79936.5
31	86494.5
32	94162.0
33	101072.0
34	107670.5
35	113373.0
36	115786.5
37	116941.0
38	117138.0
39	115752.0
40	116407.0
41	115798.0
42	113638.0
43	112940.5
44	112309.5
45	109902.5
46	107480.5
47	104475.5
48	100373.0
49	97618.5
50	97334.5
51	94044.0
52	85736.5
53	79132.0
54	75257.5
55	72855.0
56	67186.5
57	59253.5
58	54142.5
59	50013.5
60	44446.0
61	37170.5
62	31333.5
63	25875.0
64	20005.5
65	15855.0
66	12448.5
67	9424.0
68	7211.0
69	5383.5
70	3955.0
71	2964.5
72	2191.5
73	1679.5
74	1410.0
75	1065.5
76	657.0
77	502.0
78	374.5
79	210.5
80	105.0
81	49.5
82	25.0
83	18.5
84	10.5
85	7.5
86	5.0
87	2.0
88	1.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0057243072606103125
2	0.004321290775166608
3	9.259908803928446E-4
4	0.0
5	0.042399158190108734
6	0.0
7	0.0
8	0.0
9	8.418098912662224E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.2627148368993336E-4
20-21	0.0
22-23	0.0
24-25	4.209049456331112E-5
26-27	8.418098912662224E-5
28-29	1.403016485443704E-4
30-31	1.6836197825324448E-4
32-33	0.0010101718695194669
34-35	7.155384075762891E-4
36-37	9.119607155384075E-4
38-39	7.435987372851631E-4
40-41	1.6836197825324448E-4
42-43	4.910557699052964E-4
44-45	1.8239214310768152E-4
46-47	7.01508242721852E-5
48-49	8.418098912662224E-5
50-51	2.3851280252542968E-4
52-53	5.471764293230445E-4
54-55	5.892669238863557E-4
56-57	0.001781830936513504
58-59	4.489652753419853E-4
60-61	8.558400561206594E-4
62-63	8.418098912662223E-4
64-65	0.0011364433532094003
66-67	3.226937916520519E-4
68-69	1.1224131883549632E-4
70-71	2.9463346194317787E-4
72-73	1.1224131883549632E-4
74-75	4.629954401964223E-4
76-77	5.331462644686075E-4
78-79	4.910557699052964E-4
80-81	6.173272535952298E-4
82-83	0.001038232199228341
84-85	9.400210452472816E-4
86-87	0.0013048053314626447
88-89	0.001024202034373904
90-91	9.259908803928446E-4
92-93	9.680813749561557E-4
94-95	6.032970887407927E-4
96-97	1.1224131883549632E-4
98-99	3.086636267976149E-4
100	1.9642230796211859E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3563750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.511061058948606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.56472103491906	38.83015218025127
2	16.031780085526865	17.15755126080529
3	5.235138672577835	8.40413475481127
4	2.214627735596152	4.740283199293072
5	1.1345609028386903	3.0355778873448513
6	0.6538404730585101	2.0992618485987466
7	0.4314756687332854	1.6162104598526263
8	0.28847849101928297	1.2349432117700965
9	0.21583008065817372	1.0394366962011605
>10	1.1009494932076749	10.743974049636828
>50	0.08162324596765023	3.0234361913430727
>100	0.04397637038229045	4.16598159385202
>500	0.0018407209357965786	0.6792204800914075
>1k	8.940644472291015E-4	0.8552521342985935
>5k	1.0518405261518838E-4	0.30061873308909426
>10k+	1.577760789227826E-4	2.073965318760607
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32384	0.9087057172921782	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25234	0.7080743598737285	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16077	0.4511259207295686	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5661	0.15884952648193618	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5021	0.14089091546825677	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4983	0.13982462293931952	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4147	0.11636618730270079	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.806032970887408E-5	0.0	0.0	0.0
6	0.0	2.806032970887408E-5	0.0	0.0	0.0
7	0.0	2.806032970887408E-5	0.0	0.0	0.0
8	0.0	2.806032970887408E-5	0.0	0.0	0.0
9	0.0	2.806032970887408E-5	0.0	0.0	0.0
10-11	0.0	2.806032970887408E-5	0.0	0.0	0.0
12-13	0.0	2.806032970887408E-5	0.0	0.0	0.0
14-15	0.0	2.806032970887408E-5	0.0	0.0	0.0
16-17	0.0	2.806032970887408E-5	0.0	1.403016485443704E-5	0.0
18-19	0.0	2.806032970887408E-5	0.0	4.209049456331112E-5	0.0
20-21	0.0	2.806032970887408E-5	0.0	5.612065941774816E-5	0.0
22-23	0.0	2.806032970887408E-5	0.0	1.8239214310768152E-4	0.0
24-25	0.0	2.806032970887408E-5	0.0	6.453875833041039E-4	0.0
26-27	0.0	2.806032970887408E-5	0.0	0.0012065941774815855	0.0
28-29	0.0	2.806032970887408E-5	0.0	0.002188705717292178	0.0
30-31	0.0	2.806032970887408E-5	0.0	0.0047702560505085935	0.0
32-33	0.0	2.806032970887408E-5	0.0	0.009680813749561557	0.0
34-35	0.0	2.806032970887408E-5	0.0	0.01616274991231147	0.0
36-37	0.0	2.806032970887408E-5	0.0	0.028130480533146266	0.0
38-39	0.0	2.806032970887408E-5	0.0	0.047702560505085936	0.0
40-41	0.0	2.806032970887408E-5	0.0	0.0672325499824623	0.0
42-43	0.0	2.806032970887408E-5	0.0	0.08669238863556647	0.0
44-45	0.0	2.806032970887408E-5	0.0	0.11141353910908453	0.0
46-47	0.0	2.806032970887408E-5	0.0	0.14312171168011223	0.0
48-49	0.0	2.806032970887408E-5	0.0	0.17802876183795158	0.0
50-51	0.0	2.806032970887408E-5	0.0	0.2167800771659067	0.0
52-53	0.0	2.806032970887408E-5	0.0	0.2545633111189056	0.0
54-55	0.0	2.806032970887408E-5	0.0	0.29847772711329357	0.0
56-57	0.0	2.806032970887408E-5	0.0	0.3505717292178183	0.0
58-59	0.0	2.806032970887408E-5	0.0	0.41081725710277095	0.0
60-61	0.0	2.806032970887408E-5	0.0	0.478554893019993	0.0
62-63	0.0	2.806032970887408E-5	0.0	0.5497299193265521	0.0
64-65	0.0	2.806032970887408E-5	0.0	0.6231076815152579	0.0
66-67	0.0	5.612065941774816E-5	0.0	0.7074710627849877	0.0
68-69	0.0	8.418098912662224E-5	0.0	0.8090634864959663	0.0
70-71	0.0	8.418098912662224E-5	0.0	0.9240968081374956	0.0
72-73	0.0	8.418098912662224E-5	0.0	1.0498211153981059	0.0
74-75	0.0	8.418098912662224E-5	0.0	1.1742406173272535	0.0
76-77	0.0	8.418098912662224E-5	0.0	1.3116099614170467	0.0
78-79	0.0	8.418098912662224E-5	0.0	1.474191511750263	0.0
80-81	0.0	8.418098912662224E-5	0.0	1.6610171869519466	0.0
82-83	0.0	8.418098912662224E-5	0.0	1.8628972290424413	0.0
84-85	0.0	8.418098912662224E-5	0.0	2.063388284812347	0.0
86-87	0.0	8.418098912662224E-5	0.0	2.278428621536303	0.0
88	0.0	8.418098912662224E-5	0.0	2.4582813048053316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11120	0.0	65.4574	1
GTATCAA	22875	0.0	46.55857	1
ATCAACG	29765	0.0	35.508656	3
TATCAAC	29955	0.0	35.364388	2
TCAACGC	30130	0.0	35.124817	4
GTGGTAT	3560	0.0	34.86956	1
TGGTATC	3500	0.0	34.794132	2
CAACGCA	31015	0.0	34.137703	5
AACGCAG	32120	0.0	33.068787	6
CGCAGAG	36060	0.0	29.364378	8
ACGCAGA	36190	0.0	29.297857	7
CATGGGT	9105	0.0	27.62559	4
TATAACG	375	0.0	27.584536	2
ATAACGC	465	0.0	27.299496	3
GCAGAGT	38645	0.0	27.205584	9
GTACATG	21200	0.0	25.506718	1
TACATGG	21200	0.0	25.062067	2
ACATGGG	21355	0.0	24.30599	3
TACCTGG	2640	0.0	24.221956	2
GAGTACT	26540	0.0	23.242548	12-13
>>END_MODULE