FastQCFastQC Report
Wed 25 May 2016
SRR2431423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100541
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207840.670334628698669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.047.4726641
GTATCAA70750.038.513011
TACCTGG31100.035.407052
GTACCTG33750.033.325711
TCAACGC80050.033.211874
ATCAACG80250.033.13073
TATCAAC88000.030.5877572
CAACGCA87450.030.5619225
GTACATG168850.029.5712471
TACATGG165900.028.9893722
CATGGGT78100.028.9198724
AACGCAG96250.028.0247786
ACCTGGG38200.027.717013
CGCCGTA1057.427631E-526.85604794
ACATGGG172850.026.7343333
ATGGGTA34300.025.6530885
CGCAGAG103400.025.4510428
ATGGGTG42800.024.2963285
ACGCAGA109250.024.2606667
TATACCG1401.9157438E-523.5267625
Produced by FastQC (version 0.11.5)