##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431423_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3100541
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18549440242848	31.0	31.0	34.0	26.0	34.0
2	30.1743550561015	31.0	31.0	34.0	25.0	34.0
3	30.411056006032496	33.0	31.0	34.0	26.0	34.0
4	33.61660142536415	37.0	35.0	37.0	27.0	37.0
5	33.73758579551117	37.0	35.0	37.0	28.0	37.0
6	33.723113159929184	37.0	35.0	37.0	28.0	37.0
7	33.67330701319544	37.0	35.0	37.0	28.0	37.0
8	33.62220044824436	37.0	35.0	37.0	28.0	37.0
9	34.969822363258544	39.0	37.0	39.0	27.0	39.0
10-11	34.99008527866589	39.0	37.0	39.0	27.0	39.0
12-13	34.90375711851577	39.0	37.0	39.0	27.0	39.0
14-15	36.160499248356984	40.0	37.0	41.0	27.0	41.0
16-17	36.16711293287204	40.0	37.0	41.0	27.0	41.0
18-19	36.17272292158046	40.0	37.0	41.0	27.0	41.0
20-21	36.261047830039985	40.0	37.0	41.0	27.0	41.0
22-23	36.234483111173176	40.0	37.0	41.0	27.0	41.0
24-25	36.29127787698985	40.0	37.0	41.0	27.0	41.0
26-27	36.34137768215289	40.0	37.0	41.0	27.5	41.0
28-29	36.31939861462887	40.0	37.0	41.0	28.0	41.0
30-31	36.25411242747637	40.0	37.0	41.0	28.0	41.0
32-33	36.21203541575487	40.0	36.5	41.0	28.0	41.0
34-35	36.14073898716386	40.0	36.0	41.0	27.5	41.0
36-37	35.99702568035707	40.0	36.0	41.0	27.0	41.0
38-39	35.85934019256639	40.0	36.0	41.0	27.0	41.0
40-41	35.797885594804264	40.0	36.0	41.0	27.0	41.0
42-43	35.620271591312616	40.0	35.0	41.0	26.0	41.0
44-45	35.44020059725061	40.0	35.0	41.0	26.0	41.0
46-47	35.3003880613093	40.0	35.0	41.0	25.5	41.0
48-49	35.162377630226466	40.0	35.0	41.0	25.0	41.0
50-51	35.02367425555734	39.0	35.0	41.0	25.0	41.0
52-53	34.85239317912584	39.0	34.0	41.0	24.0	41.0
54-55	34.69430770307504	39.0	34.0	41.0	24.0	41.0
56-57	34.507001842581666	39.0	34.0	41.0	23.5	41.0
58-59	34.2947779113387	38.5	33.5	40.0	23.0	41.0
60-61	34.05963023872286	38.0	33.0	40.0	22.0	41.0
62-63	33.974895832695005	38.0	33.0	40.0	22.0	41.0
64-65	33.7422124074476	38.0	33.0	40.0	21.5	41.0
66-67	33.50144571544128	37.0	33.0	40.0	20.5	41.0
68-69	33.221011107416416	37.0	33.0	40.0	19.5	41.0
70-71	32.90306111094806	36.0	32.0	39.5	18.0	41.0
72-73	32.56663369392632	36.0	32.0	39.0	16.5	41.0
74-75	32.29177440324124	35.5	32.0	39.0	13.5	41.0
76-77	32.02941502789352	35.0	32.0	38.5	11.5	40.0
78-79	31.680222903035308	35.0	31.5	37.5	7.5	39.5
80-81	31.342087719530237	35.0	31.0	37.0	6.5	39.0
82-83	30.998933089418912	35.0	31.0	36.5	2.0	39.0
84-85	30.681300940706798	35.0	31.0	36.0	2.0	38.0
86-87	30.380481019280182	35.0	31.0	36.0	2.0	37.0
88-89	30.09772084936145	35.0	31.0	35.5	2.0	37.0
90-91	29.849672041105087	35.0	30.0	35.0	2.0	36.5
92-93	29.59715627047022	34.0	30.0	35.0	2.0	36.0
94-95	29.362639939288016	34.0	30.0	35.0	2.0	36.0
96-97	29.14006700766092	34.0	30.0	35.0	2.0	36.0
98-99	28.905997211454387	34.0	30.0	35.0	2.0	35.0
100	28.716270483118915	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5445199269975127
1101	2	0.5645051080047914
1101	3	0.5082616780803484
1101	4	0.654505857488374
1101	5	0.6132761283834824
1101	6	0.5651977925068508
1101	7	0.5184384053101496
1101	8	0.5858049282639541
1101	9	0.6575971346385927
1101	10-11	0.653655391069158
1101	12-13	0.5478327323624441
1101	14-15	0.7371596011466153
1101	16-17	0.7541105721645422
1101	18-19	0.6399179969800883
1101	20-21	0.7165055635703865
1101	22-23	0.7092655393992189
1101	24-25	0.7019551325521576
1101	26-27	0.6442701875773196
1101	28-29	0.6457437383933637
1101	30-31	0.7196631757131087
1101	32-33	0.7151768602565483
1101	34-35	0.7829031014806631
1101	36-37	0.7593986234366028
1101	38-39	0.8379528580774718
1101	40-41	0.6767891760071478
1101	42-43	0.7069343074195373
1101	44-45	0.6549920893570373
1101	46-47	0.7491666788199467
1101	48-49	0.7403513729197897
1101	50-51	0.7687270437642013
1101	52-53	0.6929589029786598
1101	54-55	0.7652408682925511
1101	56-57	0.6621201940170423
1101	58-59	0.6653210201221995
1101	60-61	0.6761392845700271
1101	62-63	0.7060373415211032
1101	64-65	0.6827954421391809
1101	66-67	0.7102003682478042
1101	68-69	0.7401464170861516
1101	70-71	0.7065041663640912
1101	72-73	0.6502338188569468
1101	74-75	0.7007267460683906
1101	76-77	0.6358144350090598
1101	78-79	0.6546258068229136
1101	80-81	0.5853934633655342
1101	82-83	0.653175403219457
1101	84-85	0.7028285837727957
1101	86-87	0.6429952130545615
1101	88-89	0.6425419382821751
1101	90-91	0.5336106486332319
1101	92-93	0.5445511842990953
1101	94-95	0.5238269729919836
1101	96-97	0.5461395748114484
1101	98-99	0.5239539860060027
1101	100	0.526632339818466
1102	1	0.04894873038908898
1102	2	0.07753581169217227
1102	3	0.08856393924520489
1102	4	0.1229597059582801
1102	5	0.1704394736294219
1102	6	0.12028455283559936
1102	7	0.018940800194855
1102	8	-0.043023093365889054
1102	9	0.05629159641619452
1102	10-11	0.10219055749897876
1102	12-13	0.10491741174267588
1102	14-15	0.0811816741339868
1102	16-17	0.1963333422599689
1102	18-19	0.07450186303526607
1102	20-21	0.12402272339566878
1102	22-23	0.06726693671831896
1102	24-25	0.10609147831542742
1102	26-27	0.07539656156333052
1102	28-29	0.12311749604238287
1102	30-31	0.11255192690643412
1102	32-33	0.09510421614771758
1102	34-35	0.06793993262080988
1102	36-37	0.09387715723092072
1102	38-39	0.10887363900528158
1102	40-41	0.06526119641324613
1102	42-43	0.07601716766678379
1102	44-45	0.0684346433964862
1102	46-47	0.02888419442342638
1102	48-49	-0.035577526713233
1102	50-51	-0.036003598978339824
1102	52-53	-0.008254854360053798
1102	54-55	-0.04783713188295735
1102	56-57	-0.04089541164223931
1102	58-59	0.022922421513989377
1102	60-61	-0.04022554274566659
1102	62-63	-0.015590317057892378
1102	64-65	0.02158297448431057
1102	66-67	0.008556312260012078
1102	68-69	-0.09058119732359415
1102	70-71	-0.16441879635968348
1102	72-73	-0.09228557852130592
1102	74-75	0.006122182042126667
1102	76-77	0.014861048352628359
1102	78-79	0.016196016289875104
1102	80-81	-0.011697402618377595
1102	82-83	-0.041519753823791916
1102	84-85	-0.0702909384041881
1102	86-87	-0.04496762465715065
1102	88-89	-0.11361627780387096
1102	90-91	-0.09562730821665966
1102	92-93	-0.17044657814792785
1102	94-95	-0.10396183497651634
1102	96-97	-0.15323650426847024
1102	98-99	-0.20711185526300824
1102	100	-0.23855662068181616
1103	1	-0.26023766907767865
1103	2	-0.20235879732065243
1103	3	-0.18064630542017213
1103	4	-0.07149471101029548
1103	5	-0.051459812666244886
1103	6	-0.08286291099939547
1103	7	-0.10205054693813054
1103	8	-0.14081013955446764
1103	9	-0.16138030551173443
1103	10-11	-0.10811307374576273
1103	12-13	-0.09425439548248704
1103	14-15	-0.09921368115202966
1103	16-17	-0.1632065047108
1103	18-19	-0.14366888241791287
1103	20-21	-0.276923937170622
1103	22-23	-0.14302418661480232
1103	24-25	-0.09246186338806694
1103	26-27	-0.12361831724949468
1103	28-29	-0.19581720271060732
1103	30-31	-0.17345929235223423
1103	32-33	-0.155631384275992
1103	34-35	-0.11381110642927439
1103	36-37	-0.21075085699467877
1103	38-39	-0.22323473997227694
1103	40-41	-0.14135829948990875
1103	42-43	-0.17979949416574215
1103	44-45	-0.36709994233134324
1103	46-47	-0.38592444370452483
1103	48-49	-0.3343339457106964
1103	50-51	-0.4708046608855483
1103	52-53	-0.5093220351743284
1103	54-55	-0.46977170594317386
1103	56-57	-0.46435904167772435
1103	58-59	-0.5192008442930316
1103	60-61	-0.3874609610561137
1103	62-63	-0.31209190730121605
1103	64-65	-0.3921558991487899
1103	66-67	-0.42363723641995676
1103	68-69	-0.44385989281505545
1103	70-71	-0.4118242739750144
1103	72-73	-0.4614735648991797
1103	74-75	-0.39760833398794304
1103	76-77	-0.42091652408290514
1103	78-79	-0.330127362774121
1103	80-81	-0.3283609495511044
1103	82-83	-0.37389077479873833
1103	84-85	-0.43987217545499746
1103	86-87	-0.457939121010007
1103	88-89	-0.5774878753500765
1103	90-91	-0.49454522415325997
1103	92-93	-0.4923018501167711
1103	94-95	-0.4791691451602631
1103	96-97	-0.3516615070718885
1103	98-99	-0.45117571631334386
1103	100	-0.4571598066545661
1104	1	-0.20810072637217303
1104	2	-0.12339344271984842
1104	3	-0.03660296055742762
1104	4	-0.004947435667034483
1104	5	0.07220782590106012
1104	6	0.03420075802079481
1104	7	0.039947947568727216
1104	8	0.028667844990408753
1104	9	-0.03774916086052116
1104	10-11	-0.06862386946558985
1104	12-13	-0.045964321255958396
1104	14-15	-0.2102307465838038
1104	16-17	-0.2211186572801438
1104	18-19	-0.2529791188184518
1104	20-21	-0.29817480664054585
1104	22-23	-0.1764819375223894
1104	24-25	-0.1915818407103913
1104	26-27	-0.13042955413598634
1104	28-29	-0.11884372035250834
1104	30-31	-0.10684624696828848
1104	32-33	-0.22470105144766706
1104	34-35	-0.1331944974096544
1104	36-37	-0.2065019122691396
1104	38-39	-0.17167812676014194
1104	40-41	-0.14084223930829864
1104	42-43	-0.19781386304968152
1104	44-45	-0.2176205523419128
1104	46-47	-0.37948843669160226
1104	48-49	-0.4069904264775417
1104	50-51	-0.43187167915156977
1104	52-53	-0.4915149574508604
1104	54-55	-0.33498776326625546
1104	56-57	-0.40321629615371535
1104	58-59	-0.44998127097647256
1104	60-61	-0.4712210492894471
1104	62-63	-0.40240839435683284
1104	64-65	-0.3824058062465241
1104	66-67	-0.2818232362865629
1104	68-69	-0.2830739158167077
1104	70-71	-0.39064917940526556
1104	72-73	-0.4522377217753757
1104	74-75	-0.3537498646361641
1104	76-77	-0.34804408127735087
1104	78-79	-0.37442104351428895
1104	80-81	-0.33521494530141993
1104	82-83	-0.3814219804319521
1104	84-85	-0.3959322379615493
1104	86-87	-0.28117731289368564
1104	88-89	-0.3794602619378473
1104	90-91	-0.3930818467032573
1104	92-93	-0.44862299181416887
1104	94-95	-0.4145174810186347
1104	96-97	-0.43846366812153903
1104	98-99	-0.38940864373523354
1104	100	-0.3730101244279602
1105	1	-0.05959110058805095
1105	2	-0.05807763047671344
1105	3	0.01497934530456746
1105	4	0.05975297532150137
1105	5	-0.0016543847846435256
1105	6	-0.12225716973174627
1105	7	-0.049214933677220074
1105	8	-0.008043165176154332
1105	9	-0.060906201743065935
1105	10-11	-0.01617253916059269
1105	12-13	-0.09774130589797636
1105	14-15	-0.07070879114361617
1105	16-17	-0.03699417101581304
1105	18-19	-0.0454299083490568
1105	20-21	0.002377324010538473
1105	22-23	-0.05856013349902156
1105	24-25	-0.10156507670432546
1105	26-27	-0.045857306677618226
1105	28-29	-0.07121880146866744
1105	30-31	-0.01129303476842125
1105	32-33	0.0413377761534619
1105	34-35	-0.039484244327248064
1105	36-37	-0.008558477270227627
1105	38-39	0.028299160715519633
1105	40-41	-0.13572002429640406
1105	42-43	-0.18679720719850224
1105	44-45	-0.2029628625301072
1105	46-47	-0.16766007848264763
1105	48-49	-0.32574989002934274
1105	50-51	-0.20775151908235046
1105	52-53	-0.2894913796875045
1105	54-55	-0.35756617555264825
1105	56-57	-0.3504345526970809
1105	58-59	-0.2449574769033589
1105	60-61	-0.2951422043835379
1105	62-63	-0.2357687664096133
1105	64-65	-0.20418549476047332
1105	66-67	-0.22350883005683642
1105	68-69	-0.19229790456859774
1105	70-71	-0.22219471260881818
1105	72-73	-0.17652876655149186
1105	74-75	-0.17310255769262284
1105	76-77	-0.177274018547525
1105	78-79	-0.2182253913403791
1105	80-81	-0.21891816831677957
1105	82-83	-0.22219691013128084
1105	84-85	-0.2679239100359574
1105	86-87	-0.29163048183160356
1105	88-89	-0.18858853362290162
1105	90-91	-0.2359241568914463
1105	92-93	-0.2103399504990051
1105	94-95	-0.19181414984839762
1105	96-97	-0.1693527012169298
1105	98-99	-0.24828761842699265
1105	100	-0.1323601136124708
1106	1	-0.19098848232394872
1106	2	-0.24416340302592587
1106	3	-0.18679185366141837
1106	4	-0.049805361035055284
1106	5	-0.010890659438096861
1106	6	-0.07401093364347844
1106	7	-0.04746010409814261
1106	8	-0.09377064793593348
1106	9	-0.10534397751901992
1106	10-11	-0.15331564014760346
1106	12-13	-0.16808683575951022
1106	14-15	-0.12414869397316863
1106	16-17	-0.07228918847549437
1106	18-19	-0.11015964751558016
1106	20-21	-0.21824292936786094
1106	22-23	-0.14079351630417847
1106	24-25	-0.08711833321110163
1106	26-27	-0.24569207530144865
1106	28-29	-0.19487904701775705
1106	30-31	-0.12614260512035003
1106	32-33	-0.16839996518509537
1106	34-35	-0.15494262605867704
1106	36-37	-0.1811204018645256
1106	38-39	-0.23102928380558296
1106	40-41	-0.20871681021607458
1106	42-43	-0.1932053894614043
1106	44-45	-0.18443136777905522
1106	46-47	-0.2018607499497449
1106	48-49	-0.2701534954006206
1106	50-51	-0.26934734464931154
1106	52-53	-0.2962139683412417
1106	54-55	-0.27701169065136355
1106	56-57	-0.2889830180279347
1106	58-59	-0.39937221447046767
1106	60-61	-0.4350548205821312
1106	62-63	-0.3104727836348502
1106	64-65	-0.3107546917633286
1106	66-67	-0.2369509461600785
1106	68-69	-0.2648499412430212
1106	70-71	-0.31329474691732173
1106	72-73	-0.2829080077751769
1106	74-75	-0.3308669432005189
1106	76-77	-0.2920420641969379
1106	78-79	-0.22826915643378243
1106	80-81	-0.26783835268689415
1106	82-83	-0.2963700961272444
1106	84-85	-0.30128705443499015
1106	86-87	-0.4460752560268233
1106	88-89	-0.4260233604694541
1106	90-91	-0.32197550585367907
1106	92-93	-0.28583472511542496
1106	94-95	-0.3596751695184963
1106	96-97	-0.36311553669672136
1106	98-99	-0.3951374528250504
1106	100	-0.2943072089140202
1107	1	-0.4302526881511497
1107	2	-0.5515180853200583
1107	3	-0.4246606425776811
1107	4	-0.32481296111389923
1107	5	-0.26095343288405815
1107	6	-0.3613810427207511
1107	7	-0.41853649285849315
1107	8	-0.4036476444859005
1107	9	-0.38042195789433464
1107	10-11	-0.30232639293868857
1107	12-13	-0.3755109999743027
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2216	88-89	0.333709784237616
2216	90-91	0.3289640145259334
2216	92-93	0.40836776872806624
2216	94-95	0.3368979628855939
2216	96-97	0.2370258005550845
2216	98-99	0.3276933219954117
2216	100	0.36362408557003434
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91863.0
3	17966.0
4	13149.0
5	11732.0
6	7930.0
7	6794.0
8	7254.0
9	7724.0
10	8211.0
11	8895.0
12	10019.0
13	10942.0
14	11617.0
15	12006.0
16	11403.0
17	11391.0
18	11045.0
19	10873.0
20	10552.0
21	10976.0
22	13131.0
23	20016.0
24	31893.0
25	41181.0
26	43907.0
27	34320.0
28	32217.0
29	38074.0
30	45834.0
31	52299.0
32	66481.0
33	88125.0
34	121970.0
35	186450.0
36	300931.0
37	525024.0
38	789021.0
39	376217.0
40	1108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.065271050550265	22.22264940475883	14.018407762172332	21.69367178251857
2	14.409022199808941	23.326323978229542	40.646969183497895	21.617684638463626
3	16.587015123722956	29.838617174684217	31.294865585396032	22.2795021161968
4	11.239783644915068	19.854572216683327	40.230777034208835	28.67486710419277
5	11.172151466821074	41.01101957570885	34.81312216141882	13.003706796051254
6	25.95230356746903	41.751533016622325	19.590498411549806	12.705665004358838
7	23.852694290333503	36.33556761474348	23.66290893821228	16.148829156710736
8	23.268178122965814	38.24929379419938	21.50196169174411	16.98056639109069
9	24.4999394922187	19.32821840888738	21.94631749643004	34.22552460246388
10-11	21.278214999457777	30.30302883597474	29.853289748872946	18.565466415694537
12-13	22.294512816317074	28.572288469324803	29.20482982286357	19.928368891494554
14-15	20.306053717948014	30.14681890226801	26.713336343447335	22.83379103633664
16-17	19.3159159243476	33.15242709814361	27.33768986400647	20.193967113502325
18-19	19.507096165353207	31.77200159822672	29.2430789230947	19.477823313325377
20-21	21.617496553858725	30.70394878893672	28.243237704190527	19.43531695301403
22-23	20.9561138492194	30.874802199423	28.471938702234567	19.69714524912303
24-25	20.578321006548556	30.71174333005376	28.484966529443007	20.224969133954684
26-27	20.209796111887602	30.817584181730773	28.551420171081855	20.42119953529977
28-29	20.27846146000959	30.67378255613026	28.718429856784976	20.329326127075174
30-31	20.575639043966923	31.21289652485701	28.25793243371039	19.953531997465674
32-33	20.04327800640375	30.921052737747836	28.93878190599129	20.096887349857127
34-35	20.342911605033585	30.57098626220186	28.72424495687139	20.361857175893164
36-37	20.651746570544873	30.754509179106353	28.326695747662857	20.267048502685913
38-39	20.363165118848098	30.72866225385731	28.739218459884885	20.168954167409712
40-41	20.144949988864898	30.42729746216486	28.937565963380035	20.49018658559021
42-43	20.09670732880298	30.472733645900508	28.870552518321844	20.560006506974663
44-45	20.51719819076505	30.627696195209374	28.71264708426784	20.142458529757732
46-47	20.399907623498297	30.999271994410933	28.457982167332833	20.142838214757937
48-49	20.33063709422053	30.47547481148437	28.73034348724136	20.463544607053734
50-51	20.26773647639973	30.391695482519886	28.684951297899598	20.655616743180786
52-53	20.29633086880481	30.542401236089116	28.669917952874265	20.491349942231814
54-55	20.449499856364042	30.912669337111982	28.47824964252399	20.159581163999988
56-57	20.332551127791277	30.614818074676986	28.303801725499916	20.748829072031818
58-59	20.216195331428068	30.30304595300699	28.763494657009925	20.717264058555013
60-61	20.57141510725048	30.60757195865428	28.28678109127717	20.53423184281808
62-63	20.355794243517664	30.558833211765997	28.466960579908466	20.61841196480787
64-65	20.281443621389084	30.369096811945205	28.698496327235134	20.650963239430574
66-67	20.17403844028017	30.44012137473036	28.45894662574796	20.92689355924151
68-69	20.6658591499838	30.76340019390728	28.138309340211748	20.43243131589717
70-71	20.573473972565605	30.308931460481602	28.3062972988063	20.811297268146493
72-73	20.263223247116063	30.463309641347756	28.781849401331865	20.491617710204313
74-75	20.26071511159085	30.61934886900325	28.54520799905888	20.574728020347017
76-77	20.359341833868548	30.56451887509681	28.409110164670636	20.667029126364007
78-79	20.44886396186391	30.522959193965228	28.65622410439012	20.371952739780742
80-81	20.3800281166387	30.409640801951994	28.5944375792707	20.615893502138604
82-83	20.14388721421791	30.39521374407262	28.943995408830066	20.516903632879412
84-85	20.222904442595254	30.446080023137107	28.56941809277695	20.76159744149069
86-87	20.442591245328718	30.36417116338399	28.513087981160588	20.6801496101267
88-89	20.245777291462915	30.215291159505895	28.706251835784812	20.832679713246378
90-91	20.137645549396016	30.243223833385784	28.568915571263727	21.050215045954474
92-93	20.359167317665364	30.37783959015564	28.49968750448814	20.763305587690855
94-95	20.252169158891775	30.31658552895719	28.65810363680257	20.773141675348466
96-97	20.143826148642084	30.502558849339174	28.51794583314372	20.83566916887502
98-99	20.18810748455018	30.31679451341604	28.550900360550912	20.944197641482866
100	20.245362334886728	30.618773904928716	28.285520516961384	20.850343243223175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17876.0
1	12992.0
2	6819.0
3	4574.5
4	3043.0
5	2195.0
6	1850.5
7	1760.0
8	1799.5
9	1770.5
10	1533.0
11	1382.5
12	1355.0
13	1396.0
14	1462.0
15	1582.5
16	1807.5
17	2196.0
18	2821.0
19	3688.0
20	5050.5
21	7188.5
22	11046.0
23	17588.5
24	23801.0
25	29027.5
26	37156.0
27	48270.5
28	63181.5
29	75419.0
30	80617.0
31	87308.5
32	95522.0
33	103064.5
34	110322.5
35	114858.5
36	115208.5
37	114102.0
38	112073.5
39	109402.5
40	108272.5
41	103456.0
42	95998.5
43	91492.0
44	88193.0
45	85122.5
46	83266.5
47	81685.5
48	79580.0
49	77608.0
50	76797.0
51	74015.5
52	68543.0
53	63797.5
54	61307.5
55	60661.5
56	56856.5
57	50884.5
58	45829.5
59	41164.5
60	36193.0
61	30261.5
62	24962.0
63	20063.5
64	15727.0
65	12413.5
66	9742.5
67	7506.5
68	5576.0
69	4133.0
70	3096.0
71	2363.5
72	1834.5
73	1428.0
74	1207.0
75	994.5
76	738.5
77	584.5
78	454.0
79	309.0
80	246.0
81	181.0
82	122.5
83	97.5
84	75.0
85	69.0
86	68.0
87	55.5
88	41.5
89	34.0
90	31.5
91	30.0
92	22.5
93	26.5
94	22.5
95	12.0
96	13.5
97	16.0
98	16.5
99	18.0
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17174422141168266
2	0.1662290548649413
3	0.18067814616868474
4	0.16961556063925617
5	0.1833550983521908
6	0.0721809516468255
7	0.07430961241925199
8	0.06256972573496045
9	0.05695780188038152
10-11	0.07060058228547857
12-13	0.06987490247669681
14-15	0.06766561061440568
16-17	0.06368243477509247
18-19	0.06795588253791839
20-21	0.06927823241169848
22-23	0.06747209599873054
24-25	0.0668109210618405
26-27	0.07093923286291005
28-29	0.06847192151305208
30-31	0.07353555395655145
32-33	0.07097148529885591
34-35	0.07068121337534321
36-37	0.08150190563517787
38-39	0.07622863235803042
40-41	0.0719551845952045
42-43	0.07505141844600667
44-45	0.08569472230813913
46-47	0.0758093506907343
48-49	0.08134064345544859
50-51	0.07805089498897128
52-53	0.07684142864100169
54-55	0.0784056717843757
56-57	0.07258410709614871
58-59	0.07943774973464308
60-61	0.07653503049951606
62-63	0.06840741664116037
64-65	0.07076184446520785
66-67	0.07103599017074762
68-69	0.06943949459142776
70-71	0.06521442548252063
72-73	0.06303738605617536
74-75	0.06777849414021618
76-77	0.07306789363533654
78-79	0.06977814516885925
80-81	0.07661566158938068
82-83	0.07805089498897128
84-85	0.08021180819734362
86-87	0.07595448665249065
88-89	0.07776062306545857
90-91	0.07630926344789507
92-93	0.0676172319604869
94-95	0.06789137766602668
96-97	0.06702056189548856
98-99	0.07629313722992213
100	0.0835983139716585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3100541.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.20474096938183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49725108457281	54.90294158970579
2	13.06884305815928	17.827141111025153
3	3.3979002539996612	6.9525871999153095
4	1.2763025939447634	3.4819955127421087
5	0.5780930950997374	1.9714344903732905
6	0.3143566005750466	1.2864366308541888
7	0.19235104883798998	0.9183477422828814
8	0.12731089151614736	0.6946565102751922
9	0.08383492391714739	0.5146145342961184
>10	0.4131473622030246	4.901614160461001
>50	0.028468400902484245	1.342317274208959
>100	0.01921157019697203	2.7573242060479406
>500	0.0020787275380375688	0.9590709853534156
>1k	8.031447293162122E-4	0.8198052673961139
>5k	0.0	0.0
>10k+	4.7243807606836015E-5	0.6697127850625277
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20784	0.670334628698669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.22524359458559E-5	0.0
10-11	0.0	0.0	0.0	3.22524359458559E-5	0.0
12-13	0.0	0.0	0.0	3.22524359458559E-5	0.0
14-15	0.0	0.0	0.0	9.67573078375677E-5	0.0
16-17	0.0	0.0	0.0	1.290097437834236E-4	0.0
18-19	1.612621797292795E-5	0.0	0.0	1.290097437834236E-4	0.0
20-21	6.45048718917118E-5	0.0	0.0	1.7738839770220746E-4	0.0
22-23	1.1288352581049566E-4	0.0	0.0	2.2576705162099132E-4	0.0
24-25	1.290097437834236E-4	0.0	0.0	6.127962829712621E-4	0.0
26-27	1.290097437834236E-4	0.0	0.0	0.0011127090401320285	0.0
28-29	1.290097437834236E-4	0.0	0.0	0.0024189326959391925	0.0
30-31	1.290097437834236E-4	0.0	0.0	0.005482914110795503	0.0
32-33	1.290097437834236E-4	1.612621797292795E-5	0.0	0.011707634248345693	0.0
34-35	1.290097437834236E-4	3.22524359458559E-5	0.0	0.022044539968992506	0.0
36-37	1.290097437834236E-4	3.22524359458559E-5	0.0	0.03738057326124699	0.0
38-39	1.6126217972927952E-4	3.22524359458559E-5	0.0	0.0612151234252345	0.0
40-41	1.6126217972927952E-4	3.22524359458559E-5	0.0	0.08958114083961477	0.0
42-43	1.6126217972927952E-4	3.22524359458559E-5	0.0	0.11718922600926741	0.0
44-45	1.6126217972927952E-4	6.45048718917118E-5	0.0	0.15069950695701168	0.0
46-47	1.6126217972927952E-4	6.45048718917118E-5	0.0	0.19088604214554816	0.0
48-49	1.935146156751354E-4	6.45048718917118E-5	0.0	0.23791009375460606	0.0
50-51	1.935146156751354E-4	6.45048718917118E-5	0.0	0.2857082038263645	0.0
52-53	1.935146156751354E-4	6.45048718917118E-5	0.0	0.3346512753742008	0.0
54-55	1.935146156751354E-4	6.45048718917118E-5	0.0	0.3887063580194553	0.0
56-57	1.935146156751354E-4	6.45048718917118E-5	0.0	0.45548502664534996	0.0
58-59	1.935146156751354E-4	6.45048718917118E-5	0.0	0.5309879791945986	0.0
60-61	2.0964083364806337E-4	6.45048718917118E-5	0.0	0.6126672732274787	0.0
62-63	2.2576705162099132E-4	6.45048718917118E-5	0.0	0.6972976651494046	0.0
64-65	2.2576705162099132E-4	8.063108986463974E-5	0.0	0.788749447273879	0.0
66-67	2.2576705162099132E-4	9.67573078375677E-5	0.0	0.8983109721819516	0.0
68-69	2.2576705162099132E-4	1.1288352581049566E-4	0.0	1.0191608496710736	0.0
70-71	2.2576705162099132E-4	1.290097437834236E-4	0.0	1.1567658676340677	0.0
72-73	2.2576705162099132E-4	1.290097437834236E-4	0.0	1.310690618185665	0.0
74-75	2.2576705162099132E-4	1.290097437834236E-4	0.0	1.4659377186110425	0.0
76-77	2.2576705162099132E-4	1.290097437834236E-4	0.0	1.6271031410324843	0.0
78-79	2.2576705162099132E-4	1.6126217972927952E-4	0.0	1.8198114458089734	0.0
80-81	2.2576705162099132E-4	1.6126217972927952E-4	0.0	2.0330645522829727	0.0
82-83	2.2576705162099132E-4	1.6126217972927952E-4	0.0	2.266265790389484	0.0
84-85	2.2576705162099132E-4	1.6126217972927952E-4	0.0	2.504079126836252	0.0
86-87	2.2576705162099132E-4	1.6126217972927952E-4	0.0	2.7587121086287842	0.0
88	2.2576705162099132E-4	1.6126217972927952E-4	0.0	2.960902629573355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3995	0.0	47.472664	1
GTATCAA	7075	0.0	38.51301	1
TACCTGG	3110	0.0	35.40705	2
GTACCTG	3375	0.0	33.32571	1
TCAACGC	8005	0.0	33.21187	4
ATCAACG	8025	0.0	33.1307	3
TATCAAC	8800	0.0	30.587757	2
CAACGCA	8745	0.0	30.561922	5
GTACATG	16885	0.0	29.571247	1
TACATGG	16590	0.0	28.989372	2
CATGGGT	7810	0.0	28.919872	4
AACGCAG	9625	0.0	28.024778	6
ACCTGGG	3820	0.0	27.71701	3
CGCCGTA	105	7.427631E-5	26.856047	94
ACATGGG	17285	0.0	26.734333	3
ATGGGTA	3430	0.0	25.653088	5
CGCAGAG	10340	0.0	25.451042	8
ATGGGTG	4280	0.0	24.296328	5
ACGCAGA	10925	0.0	24.260666	7
TATACCG	140	1.9157438E-5	23.526762	5
>>END_MODULE