FastQCFastQC Report
Wed 25 May 2016
SRR2431423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100541
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234190.7553197974159993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180720.5828660224135078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118290.38151406480352945No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.24511851318850483No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.13520221148502792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.12039834338588007No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.11472191465940944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90200.063.0193371
GTATCAA180300.045.869521
TATAACG3900.039.7822532
ATAACGC4150.036.2522473
GTGGTAT29150.034.8392871
TATCAAC237250.034.639792
ATCAACG236850.034.638183
TCAACGC239650.034.291784
TACCTGG29350.034.1201972
CAACGCA246100.033.4121325
TGGTATC30500.032.5254022
AACGCAG260050.031.7717386
GTACCTG32500.031.5374951
CGCAGAG288050.028.4712498
ACCTGGG34600.028.2631323
ACGCAGA294100.028.029387
GCAGAGT309300.026.2264679
GTACATG163450.026.147671
CATGGGT73250.026.1223854
TACATGG164400.025.6239592
Produced by FastQC (version 0.11.5)