##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431423_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3100541
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62062007888301	34.0	31.0	34.0	31.0	34.0
2	32.70691534154846	34.0	31.0	34.0	31.0	34.0
3	32.808703706869224	34.0	31.0	34.0	31.0	34.0
4	36.29538425713448	37.0	37.0	37.0	35.0	37.0
5	36.212450343343306	37.0	37.0	37.0	35.0	37.0
6	36.260592586906604	37.0	37.0	37.0	35.0	37.0
7	36.208679711056874	37.0	37.0	37.0	35.0	37.0
8	36.21924044868299	37.0	37.0	37.0	35.0	37.0
9	38.003778050346696	39.0	39.0	39.0	35.0	39.0
10-11	37.98296361828468	39.0	38.5	39.0	35.0	39.0
12-13	37.97627220539899	39.0	39.0	39.0	35.0	39.0
14-15	39.4246804025491	41.0	39.0	41.0	36.0	41.0
16-17	39.4646150462129	41.0	39.0	41.0	36.0	41.0
18-19	39.544748480990904	41.0	39.5	41.0	36.5	41.0
20-21	39.53376894548403	41.0	40.0	41.0	36.5	41.0
22-23	39.46510205799568	41.0	39.0	41.0	36.0	41.0
24-25	39.41665696405885	41.0	39.0	41.0	36.0	41.0
26-27	39.29377727951348	41.0	39.0	41.0	35.5	41.0
28-29	39.203368379905314	41.0	39.0	41.0	35.0	41.0
30-31	39.00589961558322	41.0	39.0	41.0	35.0	41.0
32-33	38.76011234813538	41.0	38.0	41.0	35.0	41.0
34-35	38.606063748229744	41.0	38.0	41.0	35.0	41.0
36-37	38.44588186384247	41.0	38.0	41.0	35.0	41.0
38-39	38.28956462759241	40.5	38.0	41.0	34.5	41.0
40-41	38.14099523276744	40.0	38.0	41.0	34.0	41.0
42-43	37.96206710377318	40.0	38.0	41.0	34.0	41.0
44-45	37.699461803601366	40.0	38.0	41.0	33.0	41.0
46-47	37.3031533851673	40.0	38.0	41.0	32.5	41.0
48-49	36.95120899868765	40.0	37.5	41.0	31.0	41.0
50-51	36.78561821953008	40.0	37.0	41.0	31.0	41.0
52-53	36.73000211898504	40.0	37.0	41.0	31.0	41.0
54-55	36.59928525376701	40.0	37.0	41.0	31.0	41.0
56-57	36.463925972918915	40.0	36.0	41.0	30.5	41.0
58-59	36.29941290890848	40.0	36.0	41.0	30.0	41.0
60-61	36.15771795954319	40.0	35.0	41.0	30.0	41.0
62-63	36.00245457163766	39.0	35.0	41.0	30.0	41.0
64-65	35.78074745665353	39.0	35.0	41.0	29.5	41.0
66-67	35.5313205018092	39.0	35.0	41.0	29.0	41.0
68-69	35.24623928533762	38.0	35.0	41.0	29.0	41.0
70-71	34.948848926687305	37.0	35.0	40.0	28.5	41.0
72-73	34.63411820711289	37.0	35.0	40.0	28.0	41.0
74-75	34.3022369321999	36.5	35.0	39.0	28.0	41.0
76-77	33.96843325084235	36.0	35.0	39.0	28.0	41.0
78-79	33.64069125355866	36.0	34.0	38.5	27.5	40.0
80-81	33.3645446068928	35.0	34.0	37.0	27.0	39.0
82-83	33.14408162962528	35.0	34.0	37.0	28.0	39.0
84-85	32.8636854019992	35.0	34.0	36.5	27.0	39.0
86-87	32.6069956823664	35.0	34.0	36.0	27.0	37.0
88-89	32.36448316600232	35.0	34.0	36.0	26.5	37.0
90-91	32.16703633333667	35.0	34.0	36.0	26.0	37.0
92-93	31.993946379035144	35.0	34.0	35.0	26.0	36.0
94-95	31.84642760731111	35.0	34.0	35.0	26.0	36.0
96-97	31.700352777144374	35.0	34.0	35.0	25.0	36.0
98-99	31.58020116489348	35.0	34.0	35.0	25.0	36.0
100	31.473150330861614	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.023292337496002347
1101	2	0.014297801876175242
1101	3	0.04362585342875036
1101	4	0.09273098782452394
1101	5	0.09382630322713936
1101	6	0.07971517563105124
1101	7	0.04078655009091392
1101	8	0.1460663326820466
1101	9	0.1041865470168375
1101	10-11	0.10773984454959162
1101	12-13	0.11914016913372905
1101	14-15	0.12688664385126458
1101	16-17	0.0784086379050919
1101	18-19	0.0432083197489348
1101	20-21	0.11112261903181064
1101	22-23	0.07160872818130315
1101	24-25	0.12137769076821314
1101	26-27	0.15680030494235098
1101	28-29	0.11293148489136229
1101	30-31	0.04098055521028954
1101	32-33	0.057992459315336475
1101	34-35	0.04445773263944375
1101	36-37	0.04082121136790562
1101	38-39	0.07044717444036053
1101	40-41	0.07103278248897027
1101	42-43	0.019574644610486303
1101	44-45	0.032656620963514627
1101	46-47	0.1643948124943222
1101	48-49	0.17758009747601733
1101	50-51	0.10436230834226734
1101	52-53	0.14372461508300205
1101	54-55	0.18129779984089822
1101	56-57	0.21742555451449164
1101	58-59	0.2055433578795416
1101	60-61	0.2067657596888992
1101	62-63	0.1734043169898456
1101	64-65	0.13779273835491068
1101	66-67	0.1754433166428342
1101	68-69	0.05112214882325361
1101	70-71	0.15898536925845264
1101	72-73	0.15833110129452166
1101	74-75	0.17234524066916634
1101	76-77	0.15416290002357158
1101	78-79	0.1835470658133218
1101	80-81	0.20137139720768005
1101	82-83	0.21956583758628767
1101	84-85	0.24221446937606572
1101	86-87	0.27293065147243567
1101	88-89	0.27979694437351554
1101	90-91	0.25495599905190147
1101	92-93	0.1570737523281771
1101	94-95	0.1784066990821067
1101	96-97	0.20537691054015283
1101	98-99	0.22985070604796576
1101	100	0.3018942413077781
1102	1	-0.06011437690835919
1102	2	-0.02469952227228589
1102	3	-0.025353953322309053
1102	4	0.03801862368374742
1102	5	-0.009267047095107728
1102	6	-0.012089513872716395
1102	7	-0.007088744269722724
1102	8	-0.02283226277467776
1102	9	0.01532514875713531
1102	10-11	0.021169162360173743
1102	12-13	0.04404066711654053
1102	14-15	0.040579983216048277
1102	16-17	0.0017795514618512698
1102	18-19	0.018218125709701383
1102	20-21	0.05801797185488766
1102	22-23	0.07419565941739847
1102	24-25	0.09025917124768768
1102	26-27	0.08489495222109156
1102	28-29	0.08456050103229984
1102	30-31	0.04480357083049569
1102	32-33	0.02631384852785601
1102	34-35	0.021651928480366678
1102	36-37	0.039035581064020164
1102	38-39	-0.010307882114211964
1102	40-41	-0.035632798128148124
1102	42-43	-0.08393441057005191
1102	44-45	-0.06574829224707202
1102	46-47	-0.21042731052891384
1102	48-49	-0.22024850645092187
1102	50-51	-0.21427965942437055
1102	52-53	-0.2890923841136086
1102	54-55	-0.1986775555615452
1102	56-57	-0.13128643435737786
1102	58-59	-0.13021133261815976
1102	60-61	-0.1834318431476234
1102	62-63	-0.15803407806165382
1102	64-65	-0.1847926559271329
1102	66-67	-0.1287380226017163
1102	68-69	-0.12223249823246363
1102	70-71	-0.1566786409504246
1102	72-73	-0.11365105064479053
1102	74-75	-0.14132846253192355
1102	76-77	-0.1445232709293549
1102	78-79	-0.13972413966774155
1102	80-81	-0.16836447457254167
1102	82-83	-0.1136834481179605
1102	84-85	-0.1529010227813501
1102	86-87	-0.17178280841476123
1102	88-89	-0.16527966333131872
1102	90-91	-0.09768734373103172
1102	92-93	-0.13329269727403315
1102	94-95	-0.13915222516642345
1102	96-97	-0.11478484781285303
1102	98-99	-0.1371751327192534
1102	100	-0.0667267327332155
1103	1	-0.05726262135294036
1103	2	-0.09022436631697417
1103	3	-0.042359352586920807
1103	4	0.012086197507230168
1103	5	-0.07855160517513582
1103	6	-0.03340043573285101
1103	7	-0.05114402363707171
1103	8	-0.028714892975031603
1103	9	-0.024658684646368556
1103	10-11	0.007005352954472244
1103	12-13	-0.010225607680638404
1103	14-15	-0.017398968141890236
1103	16-17	-0.010932012136244396
1103	18-19	-0.058375882063749884
1103	20-21	-0.048553032951538455
1103	22-23	-0.032186108896091525
1103	24-25	-0.013014308589511359
1103	26-27	-0.021199230711410166
1103	28-29	-0.022021852651462837
1103	30-31	0.013060978242734222
1103	32-33	-0.009236630457543527
1103	34-35	-0.038292672578592146
1103	36-37	-0.0017461025626985816
1103	38-39	-0.15758588527587136
1103	40-41	-0.11255973849882395
1103	42-43	-0.0021049000698241116
1103	44-45	-0.08879834504590889
1103	46-47	-0.014842835136320787
1103	48-49	-0.015643919205729162
1103	50-51	-0.03927907749257287
1103	52-53	0.010564218175460383
1103	54-55	0.034179892783917865
1103	56-57	0.04260917741326864
1103	58-59	0.048606697744098426
1103	60-61	0.03311437088137836
1103	62-63	0.0719583341272596
1103	64-65	0.035512451154595226
1103	66-67	0.06972491442785866
1103	68-69	0.016239114168698165
1103	70-71	0.00627252400942524
1103	72-73	-0.0019772766833199285
1103	74-75	0.05469465904294424
1103	76-77	0.059390700910164185
1103	78-79	0.023864687319054667
1103	80-81	0.03342126886150254
1103	82-83	0.036310293096924795
1103	84-85	0.024770239746004563
1103	86-87	0.04947096822600372
1103	88-89	0.047184866597696384
1103	90-91	0.008198502789454665
1103	92-93	-0.025942024594680646
1103	94-95	-0.03829023688368238
1103	96-97	-0.021162863752859806
1103	98-99	-0.06720828979244331
1103	100	-0.06833725915982569
1104	1	-0.09374238771565047
1104	2	-0.02994623122278739
1104	3	-0.07048377492538549
1104	4	-0.025602584663161565
1104	5	-0.039215022644576436
1104	6	-0.06654158047934544
1104	7	-0.09044963874536194
1104	8	-0.11913862259608976
1104	9	-0.04817629796330891
1104	10-11	-0.08737889558529588
1104	12-13	-0.08944415669015626
1104	14-15	-0.07728169689585229
1104	16-17	-0.06996547124495578
1104	18-19	-0.08139408614382404
1104	20-21	-0.06560443410828753
1104	22-23	-0.06151773663908955
1104	24-25	-0.04639028168120518
1104	26-27	-0.05680662096763456
1104	28-29	-0.013307215065580635
1104	30-31	-0.0433109500271911
1104	32-33	-0.005946532409922156
1104	34-35	-0.012262743756018324
1104	36-37	0.05186703334140219
1104	38-39	-0.062684671352919
1104	40-41	-0.2164466502369038
1104	42-43	-0.11080345841721595
1104	44-45	-0.09697436387410363
1104	46-47	-0.11560973143832598
1104	48-49	-0.14527576419710186
1104	50-51	-0.2443690974070094
1104	52-53	-0.171530203877154
1104	54-55	-0.06894912702569655
1104	56-57	-0.11153328702183529
1104	58-59	-0.10071399611672405
1104	60-61	-0.15332990205119046
1104	62-63	-0.19623204882065437
1104	64-65	-0.13850885919615052
1104	66-67	-0.1843044950948567
1104	68-69	-0.1891546723371249
1104	70-71	-0.19483201247972914
1104	72-73	-0.16725817502442197
1104	74-75	-0.16340570399052723
1104	76-77	-0.16668860904037786
1104	78-79	-0.18051317716750503
1104	80-81	-0.23614797429469547
1104	82-83	-0.18921710592545793
1104	84-85	-0.19684218628950134
1104	86-87	-0.1961521915944786
1104	88-89	-0.13060148677394068
1104	90-91	-0.12455481072473162
1104	92-93	-0.13530009375208252
1104	94-95	-0.1831916890175762
1104	96-97	-0.13646553457392585
1104	98-99	-0.09443565737098325
1104	100	-0.024375668291877872
1105	1	-0.15259919065746175
1105	2	-0.0910559002688629
1105	3	-0.047985397248552886
1105	4	-0.010936783235514724
1105	5	-0.0907064784092313
1105	6	0.02268835679082315
1105	7	0.06146362104265535
1105	8	-0.003464817707488521
1105	9	-0.018098426451302885
1105	10-11	-0.024134790026131725
1105	12-13	-0.023577295344708205
1105	14-15	-0.040492274442904375
1105	16-17	-0.03934303992330257
1105	18-19	-0.08335980104757112
1105	20-21	0.017186502512949176
1105	22-23	0.012627839826407694
1105	24-25	-0.02293689643352792
1105	26-27	0.08118169830556354
1105	28-29	-0.00822339503530145
1105	30-31	0.07474929223972993
1105	32-33	0.030740142909138513
1105	34-35	0.019317399008421887
1105	36-37	0.07275931108313216
1105	38-39	0.08586334941281848
1105	40-41	0.06980524247344988
1105	42-43	0.05385909836410008
1105	44-45	0.024971342377249073
1105	46-47	0.0645535824271235
1105	48-49	0.10905637943256608
1105	50-51	0.06480189545477089
1105	52-53	0.07472601502831822
1105	54-55	0.1665608232230582
1105	56-57	0.2070114890975674
1105	58-59	0.1634115716963791
1105	60-61	0.21153706744913592
1105	62-63	0.17688876017783883
1105	64-65	0.1346443962656707
1105	66-67	0.07528397559516975
1105	68-69	0.1845328682209555
1105	70-71	0.20662591527281648
1105	72-73	0.2455159975907648
1105	74-75	0.16326637042003256
1105	76-77	0.17844063603857307
1105	78-79	0.24925483061964826
1105	80-81	0.27133796557119183
1105	82-83	0.25889559145719687
1105	84-85	0.20312915266788423
1105	86-87	0.1330571962489131
1105	88-89	0.207706970937501
1105	90-91	0.2642279061257966
1105	92-93	0.26315112839803945
1105	94-95	0.24890597483706145
1105	96-97	0.2645294903016975
1105	98-99	0.29440167769282866
1105	100	0.2872023113750046
1106	1	-0.11567494505902687
1106	2	-0.08437492557958848
1106	3	-0.09434648391989953
1106	4	-0.044688976772491174
1106	5	-0.03185411329290844
1106	6	-0.04660681174964054
1106	7	-0.05440862169270133
1106	8	-0.03989660070428869
1106	9	-0.03295613086022797
1106	10-11	-0.07775268001676494
1106	12-13	-0.12577707211156763
1106	14-15	-0.14135941463413104
1106	16-17	-0.1676348874944722
1106	18-19	-0.12333328227251883
1106	20-21	-0.13940822677462705
1106	22-23	-0.20297498471222042
1106	24-25	-0.07967604090841718
1106	26-27	-0.09671261391710573
1106	28-29	-0.10723332792956342
1106	30-31	-0.12898637125464774
1106	32-33	-0.1391418699933098
1106	34-35	-0.15572522883610418
1106	36-37	-0.1604407194644608
1106	38-39	-0.13209340309047235
1106	40-41	-0.20273506035191247
1106	42-43	-0.21857601872143562
1106	44-45	-0.11974752560139024
1106	46-47	-0.16019734027277366
1106	48-49	-0.08766431587785206
1106	50-51	-0.1554930877102194
1106	52-53	-0.20683551860646077
1106	54-55	-0.15393957365171929
1106	56-57	-0.1961709296284937
1106	58-59	-0.12603670585145466
1106	60-61	-0.1880484823226638
1106	62-63	-0.11868259333983389
1106	64-65	-0.07967152562842017
1106	66-67	-0.04965815498549375
1106	68-69	-0.0591067204139577
1106	70-71	-0.04145469815752989
1106	72-73	-0.037218989998933694
1106	74-75	-0.13550157082620018
1106	76-77	-0.15427877623599073
1106	78-79	-0.13160422799237637
1106	80-81	-0.10700261959387802
1106	82-83	-0.09671939595941836
1106	84-85	-0.047474767689791975
1106	86-87	-0.058754179066376366
1106	88-89	-0.09748158152120823
1106	90-91	-0.08639306030605098
1106	92-93	-0.10244425948577529
1106	94-95	-0.08158207272548523
1106	96-97	-0.10188648445225468
1106	98-99	-0.09556073486203331
1106	100	-0.03399774329396266
1107	1	-0.2717120802134829
1107	2	-0.20917314089057015
1107	3	-0.2173253753385893
1107	4	-0.21264764931793678
1107	5	-0.3009988856981991
1107	6	-0.25242507275950743
1107	7	-0.1845114527683478
1107	8	-0.13231270165753273
1107	9	-0.1113489013094977
1107	10-11	-0.1331395806860982
1107	12-13	-0.10081375880042742
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2216	24-25	-0.19556649913361213
2216	26-27	-0.14653436901892292
2216	28-29	-0.1552469992891119
2216	30-31	-0.10893473344766846
2216	32-33	-0.025559736498507846
2216	34-35	-0.0022674136756251073
2216	36-37	-0.02915925786954432
2216	38-39	-0.03973234381260937
2216	40-41	-0.037763371167741866
2216	42-43	-0.010949797112957071
2216	44-45	0.07800931322933025
2216	46-47	0.014942846619163674
2216	48-49	0.06400408619088438
2216	50-51	0.09344369991848112
2216	52-53	0.045648051755122765
2216	54-55	0.009920250522021945
2216	56-57	-0.004234399073716588
2216	58-59	0.008924390622446765
2216	60-61	-0.033891863591996696
2216	62-63	-0.053675660632485744
2216	64-65	-0.045083953249196895
2216	66-67	-0.038083398405703406
2216	68-69	-0.021985451035305914
2216	70-71	-0.05476375309405768
2216	72-73	-0.07439088802949101
2216	74-75	-0.08250170357153763
2216	76-77	-0.057343685286639356
2216	78-79	-0.06414953882003971
2216	80-81	-0.012969832137102344
2216	82-83	-0.007084785717360376
2216	84-85	-0.03072644647821221
2216	86-87	-0.03702372079675342
2216	88-89	0.021467680409173795
2216	90-91	0.029408445300340702
2216	92-93	0.006021624429180861
2216	94-95	-0.05244408726133898
2216	96-97	-0.027872009613858495
2216	98-99	-0.07232384989387697
2216	100	-0.006831391343823157
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	167.0
4	52.0
5	95.0
6	303.0
7	598.0
8	1183.0
9	1980.0
10	3088.0
11	4926.0
12	7931.0
13	11518.0
14	12079.0
15	14955.0
16	19410.0
17	29695.0
18	33708.0
19	16303.0
20	9903.0
21	8858.0
22	8491.0
23	8508.0
24	9251.0
25	10648.0
26	13213.0
27	16551.0
28	20791.0
29	25909.0
30	32908.0
31	41264.0
32	52904.0
33	68390.0
34	95533.0
35	153628.0
36	239954.0
37	496949.0
38	952447.0
39	669224.0
40	7194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03636985263049	21.290652406003154	14.679376977586548	21.99360076377981
2	14.121053823273469	22.296112186597497	41.48334121402165	22.09949277610739
3	15.908189297889702	29.35488032613989	32.66381982319038	22.073110552780026
4	10.600730646683918	18.84971042150386	40.8266815371898	29.722877394622422
5	10.565568235020425	39.190065167652556	35.90338525256377	14.340981344763243
6	25.631397875403035	41.1648483280821	19.498339160810968	13.705414635703898
7	23.07052220886613	35.76527451177069	24.44760446644634	16.716598812916843
8	24.461666528518734	34.98470105700908	22.129815409633352	18.42381700483883
9	25.54325196796301	15.702549974343189	23.227946348717854	35.52625170897595
10-11	21.937510260306183	27.718259490843693	30.72228362727666	19.62194662157346
12-13	23.939048056452084	26.11840965818546	30.762518541119118	19.180023744243343
14-15	21.166773798508068	27.208848391296875	29.753452703899093	21.870925106295967
16-17	18.476227213250848	30.677759139453403	30.11497025841619	20.731043388879552
18-19	18.451837172208254	27.921431092879157	34.55567974541205	19.071051989500535
20-21	20.347755440099004	27.006673996570274	34.12764095040188	18.51792961292884
22-23	19.63685358034407	27.226434750075796	34.44791874963716	18.68879291994298
24-25	19.31698682858682	27.10558046250323	34.40725899242406	19.170173716485884
26-27	19.037598505161448	27.23674668456465	34.406217893650954	19.31943691662295
28-29	19.052536009856347	27.181603849651996	34.518728995594316	19.247131144897338
30-31	19.415391214741973	27.693639968586066	33.99293992810918	18.898028888562777
32-33	18.956628229805812	27.44135131927279	34.50480181235518	19.097218638566215
34-35	19.263231600706078	27.30167556143129	34.067136631905754	19.367956205956887
36-37	19.687621502521445	27.44959162251655	33.498113127827864	19.364673747134134
38-39	19.397260450700045	27.54474263911421	33.75270520011224	19.305291710073504
40-41	19.248410718596602	27.37706845250814	33.73083980788486	19.643681021010394
42-43	19.294154908306645	27.521762850124233	33.399268414102195	19.784813827466934
44-45	19.77670620609224	27.709291367229	32.99318762509942	19.52081480157934
46-47	19.84568139644355	28.2822939987586	32.1636744128067	19.70835019199115
48-49	19.98563796942884	28.058853685483143	31.797980768812106	20.157527576275914
50-51	19.91677871743822	28.205025658524725	31.55392192751624	20.32427369652082
52-53	19.89769770654378	28.44973918865789	31.412503140605256	20.24005996419307
54-55	20.107879078544695	28.821772299608273	31.181546510838466	19.888802111008566
56-57	19.899957151678382	28.426305482891877	31.128846048093006	20.544891317336734
58-59	19.74686206165863	28.082246124282907	31.70874720168178	20.462144612376687
60-61	20.079799929301817	28.31883138999337	31.289896739368828	20.31147194133599
62-63	19.907711942182406	28.168757425190925	31.51182087976186	20.411709752864812
64-65	19.84832082268247	28.077564476470908	31.719857964454988	20.354256736391633
66-67	19.781498541297072	28.19757986420702	31.41786956643037	20.603052028065537
68-69	20.290292117610782	28.486675834985736	30.977425809506236	20.24560623789724
70-71	20.257191222091294	28.10790465576327	31.0048254598765	20.63007866226893
72-73	20.003196225679982	28.268487472472465	31.347555838804436	20.380760463043117
74-75	20.0178324753598	28.46314960411518	31.05858269717722	20.460435223347805
76-77	20.163101804779362	28.44381623390289	30.85255929050554	20.540522670812212
78-79	20.18436283116055	28.451308032064983	31.090674731129475	20.273654405644994
80-81	20.143604943686867	28.322023402526025	31.0794640898946	20.454907563892508
82-83	19.872507433894818	28.320804955467587	31.367980172195114	20.43870743844248
84-85	19.89026310858192	28.415723621975374	30.956969295666802	20.7370439737759
86-87	20.051572618132823	28.355151174051134	30.880403292068248	20.71287291574779
88-89	19.80581541030352	28.24536401910206	31.076242939251642	20.872577631342782
90-91	19.656063201184708	28.32322989287413	30.86872924032051	21.151977665620656
92-93	19.77774269389298	28.39741777590783	30.8575004946774	20.967339035521785
94-95	19.675541469744022	28.25665182883062	31.021027282686013	21.046779418739344
96-97	19.552513072330203	28.447116024237733	30.871679507182222	21.12869139624984
98-99	19.591866749297658	28.189312058271298	30.88301150947829	21.335809682952743
100	19.7292402762749	28.442607485482345	30.613748917525523	21.21440332071723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1229.0
1	768.0
2	3095.0
3	5181.5
4	2799.5
5	1391.0
6	2845.5
7	6774.0
8	11960.0
9	13122.0
10	8446.5
11	4465.5
12	3964.0
13	4340.0
14	4856.0
15	5274.0
16	5742.0
17	6298.5
18	6900.5
19	7712.0
20	8977.5
21	10786.0
22	14298.0
23	20550.0
24	26313.0
25	31190.0
26	39060.0
27	49487.0
28	63565.0
29	75745.0
30	80407.0
31	86581.5
32	94783.0
33	101598.5
34	108321.0
35	113118.0
36	113880.0
37	112292.5
38	110254.5
39	108402.5
40	106961.5
41	101769.0
42	95115.0
43	91061.5
44	87833.0
45	84649.0
46	82681.0
47	81142.0
48	78676.5
49	76317.5
50	75712.5
51	72828.5
52	66290.5
53	61250.0
54	58876.5
55	57950.5
56	53952.5
57	47931.0
58	43565.5
59	39292.5
60	34196.5
61	28384.0
62	23364.5
63	18652.0
64	14059.5
65	10868.0
66	8381.0
67	6143.5
68	4471.5
69	3236.5
70	2263.0
71	1678.0
72	1291.5
73	972.5
74	844.5
75	642.0
76	362.0
77	278.0
78	220.0
79	113.5
80	51.0
81	18.0
82	6.5
83	5.0
84	4.5
85	4.0
86	3.0
87	2.0
88	1.0
89	2.0
90	1.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005515166546741359
2	0.004354078852690547
3	9.998255143215328E-4
4	0.0
5	0.042057176473396095
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.8378653918783844E-5
20-21	0.0
22-23	3.22524359458559E-5
24-25	1.612621797292795E-5
26-27	1.7738839770220746E-4
28-29	3.22524359458559E-5
30-31	1.9351461567513538E-4
32-33	0.0014513596175635155
34-35	9.030682064839653E-4
36-37	0.001273971219861308
38-39	9.998255143215328E-4
40-41	1.4513596175635155E-4
42-43	5.321651931066224E-4
44-45	2.2576705162099132E-4
46-47	2.4189326959391926E-4
48-49	1.4513596175635155E-4
50-51	3.8702923135027075E-4
52-53	7.740584627005415E-4
54-55	0.0010482041682403167
56-57	0.002241544298236985
58-59	5.805438470254062E-4
60-61	0.0011933401299966684
62-63	0.001015951732294461
64-65	0.0015964955793198671
66-67	4.0315544932319875E-4
68-69	2.902719235127031E-4
70-71	2.4189326959391926E-4
72-73	2.902719235127031E-4
74-75	5.805438470254062E-4
76-77	7.579322447276136E-4
78-79	7.901846806734696E-4
80-81	6.77301154862974E-4
82-83	0.0014997382714822993
84-85	0.0010965828221591006
86-87	0.00188676750283257
88-89	0.0012094663479695962
90-91	9.514468604027492E-4
92-93	0.0011449614760778845
94-95	6.934273728359018E-4
96-97	1.1288352581049566E-4
98-99	3.0639814148563103E-4
100	1.9351461567513538E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3100541.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.968294783720935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5520896984766	48.83322845227243
2	14.816154832073666	18.65896010054532
3	4.073909023971545	7.695813130305032
4	1.436996649109414	3.6194091441736305
5	0.7016727998990329	2.2091569852880566
6	0.3626826861514678	1.3702506176722422
7	0.22224352749178602	0.9796007167013738
8	0.15468529516977372	0.7792215411965756
9	0.10838241589563968	0.6142190322139633
>10	0.5033093593325567	5.612242170699065
>50	0.03809214869559178	1.6734895029033459
>100	0.025216773844402216	3.305130709500012
>500	0.003128676216459063	1.3633558438058495
>1k	0.0012309545760083923	1.3184316951369215
>5k	5.128977400034968E-5	0.24545185025697777
>10k+	1.5386932200104903E-4	1.722038507329218
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23419	0.7553197974159993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18072	0.5828660224135078	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11829	0.38151406480352945	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7600	0.24511851318850483	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4192	0.13520221148502792	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3733	0.12039834338588007	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3557	0.11472191465940944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.22524359458559E-5	0.0
5	0.0	0.0	0.0	3.22524359458559E-5	0.0
6	0.0	0.0	0.0	3.22524359458559E-5	0.0
7	0.0	0.0	0.0	3.22524359458559E-5	0.0
8	0.0	0.0	0.0	6.45048718917118E-5	0.0
9	0.0	0.0	0.0	6.45048718917118E-5	0.0
10-11	1.612621797292795E-5	0.0	0.0	9.67573078375677E-5	0.0
12-13	3.22524359458559E-5	0.0	0.0	9.67573078375677E-5	0.0
14-15	3.22524359458559E-5	0.0	0.0	2.2576705162099132E-4	0.0
16-17	3.22524359458559E-5	0.0	0.0	2.2576705162099132E-4	0.0
18-19	3.22524359458559E-5	0.0	0.0	2.2576705162099132E-4	0.0
20-21	3.22524359458559E-5	0.0	0.0	3.22524359458559E-4	0.0
22-23	3.22524359458559E-5	0.0	0.0	3.870292313502708E-4	0.0
24-25	3.22524359458559E-5	0.0	0.0	7.901846806734696E-4	0.0
26-27	3.22524359458559E-5	0.0	0.0	0.001306223655807164	0.0
28-29	1.6126217972927952E-4	0.0	0.0	0.0026446997475601842	0.0
30-31	2.580194875668472E-4	0.0	0.0	0.00574093359836235	0.0
32-33	2.580194875668472E-4	0.0	0.0	0.012078537261723034	0.0
34-35	2.580194875668472E-4	0.0	0.0	0.022625083816017914	0.0
36-37	2.580194875668472E-4	0.0	0.0	0.038235262813812176	0.0
38-39	2.580194875668472E-4	0.0	0.0	0.06244071599117702	0.0
40-41	2.902719235127031E-4	0.0	0.0	0.09117763641893464	0.0
42-43	2.902719235127031E-4	0.0	0.0	0.11935013921763976	0.0
44-45	2.902719235127031E-4	0.0	0.0	0.15371510971794922	0.0
46-47	2.902719235127031E-4	0.0	0.0	0.194498314971484	0.0
48-49	2.902719235127031E-4	0.0	0.0	0.24228029882526952	0.0
50-51	2.902719235127031E-4	0.0	0.0	0.29111048684729535	0.0
52-53	2.902719235127031E-4	3.22524359458559E-5	0.0	0.3410372576914803	0.0
54-55	2.902719235127031E-4	3.22524359458559E-5	0.0	0.3963179329026773	0.0
56-57	2.902719235127031E-4	3.22524359458559E-5	0.0	0.4644995824922167	0.0
58-59	2.902719235127031E-4	3.22524359458559E-5	0.0	0.5418409238903792	0.0
60-61	3.063981414856311E-4	3.22524359458559E-5	0.0	0.625810140875415	0.0
62-63	3.2252435945855903E-4	3.22524359458559E-5	0.0	0.7124563100439569	0.0
64-65	3.2252435945855903E-4	3.22524359458559E-5	0.0	0.806423782172208	0.0
66-67	4.192816672961267E-4	3.22524359458559E-5	0.0	0.9184687446481115	0.0
68-69	4.192816672961267E-4	3.22524359458559E-5	0.0	1.0420117005387124	0.0
70-71	4.192816672961267E-4	3.22524359458559E-5	0.0	1.1836482729949385	0.0
72-73	4.192816672961267E-4	3.22524359458559E-5	0.0	1.3417819664374702	0.0
74-75	4.192816672961267E-4	3.22524359458559E-5	0.0	1.500947737830269	0.0
76-77	4.192816672961267E-4	3.22524359458559E-5	0.0	1.6662898507066992	0.0
78-79	4.192816672961267E-4	3.22524359458559E-5	0.0	1.8636908849133103	0.0
80-81	4.192816672961267E-4	3.22524359458559E-5	0.0	2.082668798767699	0.0
82-83	4.192816672961267E-4	3.22524359458559E-5	0.0	2.3221270094477062	0.0
84-85	4.192816672961267E-4	3.22524359458559E-5	0.0	2.5651813667356764	0.0
86-87	4.192816672961267E-4	3.22524359458559E-5	0.0	2.8267486222565674	0.0
88	4.192816672961267E-4	3.22524359458559E-5	0.0	3.035050979812878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9020	0.0	63.019337	1
GTATCAA	18030	0.0	45.86952	1
TATAACG	390	0.0	39.782253	2
ATAACGC	415	0.0	36.252247	3
GTGGTAT	2915	0.0	34.839287	1
TATCAAC	23725	0.0	34.63979	2
ATCAACG	23685	0.0	34.63818	3
TCAACGC	23965	0.0	34.29178	4
TACCTGG	2935	0.0	34.120197	2
CAACGCA	24610	0.0	33.412132	5
TGGTATC	3050	0.0	32.525402	2
AACGCAG	26005	0.0	31.771738	6
GTACCTG	3250	0.0	31.537495	1
CGCAGAG	28805	0.0	28.471249	8
ACCTGGG	3460	0.0	28.263132	3
ACGCAGA	29410	0.0	28.02938	7
GCAGAGT	30930	0.0	26.226467	9
GTACATG	16345	0.0	26.14767	1
CATGGGT	7325	0.0	26.122385	4
TACATGG	16440	0.0	25.623959	2
>>END_MODULE