FastQCFastQC Report
Wed 25 May 2016
SRR2431422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013650
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247660.6170443362027083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.048.445531
GTATCAA99900.037.0688441
ATCAACG115600.031.3404773
TCAACGC115300.031.254894
TATCAAC128950.028.6796552
CAACGCA126550.028.3648785
GTACATG232200.027.6814171
AACGCAG131500.027.19646
TACATGG231350.026.7034852
ACATGGG237750.025.3711553
CATGGGT101850.025.3588664
ACGCAGA148650.023.7741747
CGCAGAG148300.023.5764758
TACCTGG31700.021.6703682
ATGGGTA40950.021.5984425
GTGGTAT27200.021.2782571
GCAGAGT168450.020.3101399
CATGGGG108850.019.665344
ATGGGTG60900.019.6216225
TGGTATC30200.018.3843292
Produced by FastQC (version 0.11.5)