##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431422_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4013650
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7001076327034	33.0	31.0	34.0	26.0	34.0
2	30.71809549910929	33.0	31.0	34.0	26.0	34.0
3	30.913766521744545	34.0	31.0	34.0	27.0	34.0
4	34.13273230102276	37.0	35.0	37.0	30.0	37.0
5	34.23150274687629	37.0	35.0	37.0	31.0	37.0
6	34.23569319696536	37.0	35.0	37.0	30.0	37.0
7	34.19858433097056	37.0	35.0	37.0	30.0	37.0
8	34.15882625540343	37.0	35.0	37.0	30.0	37.0
9	35.60983468912337	39.0	37.0	39.0	30.0	39.0
10-11	35.62572496356185	39.0	37.0	39.0	30.0	39.0
12-13	35.55101690481233	39.0	37.0	39.0	30.0	39.0
14-15	36.891711908113564	41.0	38.0	41.0	29.5	41.0
16-17	36.907461537503266	40.5	38.0	41.0	30.0	41.0
18-19	36.91994630822319	40.5	38.0	41.0	30.0	41.0
20-21	36.99429397182116	41.0	38.0	41.0	30.0	41.0
22-23	36.959957146238466	40.0	38.0	41.0	30.0	41.0
24-25	36.994259340002245	40.5	38.0	41.0	30.0	41.0
26-27	37.03017066759682	40.0	38.0	41.0	30.0	41.0
28-29	37.002465087887586	40.0	38.0	41.0	30.0	41.0
30-31	36.9302457862544	40.0	38.0	41.0	30.0	41.0
32-33	36.88216660645547	40.0	38.0	41.0	30.0	41.0
34-35	36.80969989909434	40.0	38.0	41.0	30.0	41.0
36-37	36.67071045058737	40.0	37.0	41.0	29.5	41.0
38-39	36.53919325302405	40.0	37.0	41.0	29.0	41.0
40-41	36.46768776550023	40.0	37.0	41.0	29.0	41.0
42-43	36.288944227822554	40.0	36.5	41.0	28.0	41.0
44-45	36.11336127963325	40.0	36.0	41.0	28.0	41.0
46-47	35.97046154996076	40.0	36.0	41.0	27.5	41.0
48-49	35.82279371644264	40.0	35.0	41.0	27.0	41.0
50-51	35.66611724988476	40.0	35.0	41.0	27.0	41.0
52-53	35.48808304162047	39.5	35.0	41.0	27.0	41.0
54-55	35.309371519689066	39.0	35.0	41.0	27.0	41.0
56-57	35.098051648748644	39.0	35.0	41.0	26.5	41.0
58-59	34.86870554233678	39.0	34.0	41.0	26.0	41.0
60-61	34.61543383204814	38.0	34.0	40.0	26.0	41.0
62-63	34.499957395388236	38.0	34.0	40.0	26.0	41.0
64-65	34.23534189578065	37.5	34.0	40.0	25.5	41.0
66-67	33.955490762772044	37.0	34.0	40.0	25.0	41.0
68-69	33.65215825993796	36.5	33.0	40.0	24.0	41.0
70-71	33.31335828983593	36.0	33.0	39.0	23.0	41.0
72-73	32.96929004771218	35.5	33.0	39.0	22.5	41.0
74-75	32.68477545376403	35.0	33.0	39.0	22.0	40.5
76-77	32.430169172698164	35.0	33.0	37.5	21.5	40.0
78-79	32.09230476000648	35.0	33.0	37.0	20.0	39.0
80-81	31.782065202496483	35.0	32.0	37.0	20.0	39.0
82-83	31.475356595617455	35.0	32.0	36.0	18.5	39.0
84-85	31.19373164077585	35.0	32.0	36.0	18.0	37.0
86-87	30.928182203231472	35.0	32.0	35.5	16.0	37.0
88-89	30.67467417437993	35.0	32.0	35.0	11.0	37.0
90-91	30.45523501052658	35.0	31.0	35.0	7.0	36.0
92-93	30.240095548939244	35.0	31.0	35.0	2.0	36.0
94-95	30.03510769499084	35.0	31.0	35.0	2.0	36.0
96-97	29.839841042442664	35.0	31.0	35.0	2.0	35.5
98-99	29.61980404370087	35.0	31.0	35.0	2.0	35.0
100	29.447399250059174	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17180398467018065
1101	2	0.1840274241696349
1101	3	0.12629397437932965
1101	4	0.22859868337528866
1101	5	0.18309660060155153
1101	6	0.31536378158481426
1101	7	0.23546194582502977
1101	8	0.22390399028358843
1101	9	0.15657154360933845
1101	10-11	0.17630277220550283
1101	12-13	0.08522851162993561
1101	14-15	0.1593907230577969
1101	16-17	0.17910477769820687
1101	18-19	0.08316523672629472
1101	20-21	0.16112817465685936
1101	22-23	0.16959713615216288
1101	24-25	0.17060341320305383
1101	26-27	0.220908097967353
1101	28-29	0.17168053814917528
1101	30-31	0.16896008888779335
1101	32-33	0.17851797062134978
1101	34-35	0.22535805860878355
1101	36-37	0.1905658656572129
1101	38-39	0.274339539051752
1101	40-41	0.17668129416610867
1101	42-43	0.18911028602414603
1101	44-45	0.11226484804795689
1101	46-47	0.17373229757604491
1101	48-49	0.08905903898741485
1101	50-51	0.12169318542374441
1101	52-53	-0.003600696773759182
1101	54-55	0.027308787370394327
1101	56-57	-0.06430674525512359
1101	58-59	-0.002223133209803052
1101	60-61	0.03242318568428004
1101	62-63	0.003452981379595599
1101	64-65	0.07061846148653217
1101	66-67	0.0799935073005642
1101	68-69	0.11803124439953194
1101	70-71	0.0765483664883746
1101	72-73	0.05873777658228363
1101	74-75	0.12718570158654785
1101	76-77	0.1418750458337712
1101	78-79	0.17351680324205176
1101	80-81	0.05678735408800506
1101	82-83	0.04457779481173674
1101	84-85	0.07413278462373185
1101	86-87	0.06708508517640865
1101	88-89	0.006220171207534264
1101	90-91	0.025196460213109617
1101	92-93	0.019585914261551096
1101	94-95	0.052457058925973854
1101	96-97	0.01700359852567246
1101	98-99	0.044513725268515714
1101	100	0.033382013605709915
1102	1	0.049733717349749185
1102	2	0.04201833416556511
1102	3	0.06226694852411896
1102	4	0.08036510360001614
1102	5	0.09566883341010168
1102	6	0.1058521827948411
1102	7	-0.02845530626537851
1102	8	0.06214222309068873
1102	9	0.04409879024739638
1102	10-11	0.05139218138672419
1102	12-13	-0.01631137303979102
1102	14-15	0.0827101635022629
1102	16-17	0.01380564055585154
1102	18-19	-0.0309944828903852
1102	20-21	-0.05083586377751459
1102	22-23	0.024642720604205692
1102	24-25	0.0342910172210793
1102	26-27	0.06205320401215175
1102	28-29	0.038389706220506525
1102	30-31	0.06486315613440041
1102	32-33	0.10602495804121048
1102	34-35	0.12497471031034024
1102	36-37	0.08656860612583017
1102	38-39	0.09785120469423703
1102	40-41	0.07492789322615323
1102	42-43	0.018722001297362567
1102	44-45	-0.027214110486774246
1102	46-47	-0.20259467031609546
1102	48-49	-0.12513877566205878
1102	50-51	-0.1699085462985721
1102	52-53	-0.12963282328196613
1102	54-55	-0.1411921855032361
1102	56-57	-0.12127317076023303
1102	58-59	-0.15553880593380853
1102	60-61	-0.16358532565800488
1102	62-63	-0.13566292077448594
1102	64-65	-0.07459291840989124
1102	66-67	-0.09498737554500991
1102	68-69	-0.16026829046683133
1102	70-71	-0.12777044515490843
1102	72-73	-0.17235483086192716
1102	74-75	-0.1600873904463498
1102	76-77	-0.1603523297375915
1102	78-79	-0.18682708511812507
1102	80-81	-0.18395732720111369
1102	82-83	-0.20748688208837152
1102	84-85	-0.2638342534538616
1102	86-87	-0.28759296183883265
1102	88-89	-0.3378510947828737
1102	90-91	-0.2906590182171449
1102	92-93	-0.2528743199983765
1102	94-95	-0.26421730223891515
1102	96-97	-0.2768764171992011
1102	98-99	-0.3453027960706372
1102	100	-0.2728196348900731
1103	1	-0.28405536601685455
1103	2	-0.2819751194759519
1103	3	-0.21289755680523825
1103	4	-0.20836365760057163
1103	5	-0.16843729223345605
1103	6	-0.15760977499009243
1103	7	-0.23692942866943412
1103	8	-0.2488623246427224
1103	9	-0.18779756430959793
1103	10-11	-0.21665507952022267
1103	12-13	-0.22292204944270821
1103	14-15	-0.2805117076098753
1103	16-17	-0.2971079189543744
1103	18-19	-0.3098785550259251
1103	20-21	-0.3612752329346236
1103	22-23	-0.2939803606363256
1103	24-25	-0.21458184631582355
1103	26-27	-0.19429186886920746
1103	28-29	-0.2227267507358306
1103	30-31	-0.17997120245674836
1103	32-33	-0.16255265305564848
1103	34-35	-0.18983802562014063
1103	36-37	-0.3166074311294338
1103	38-39	-0.23752134938683866
1103	40-41	-0.1779256826050286
1103	42-43	-0.2691445738358169
1103	44-45	-0.34156639267933997
1103	46-47	-0.40074564401591317
1103	48-49	-0.3203435674727899
1103	50-51	-0.4199486026879029
1103	52-53	-0.3927568783091999
1103	54-55	-0.3546094071621084
1103	56-57	-0.38929027702554464
1103	58-59	-0.3954265300016715
1103	60-61	-0.3836512787876529
1103	62-63	-0.25349437750942627
1103	64-65	-0.3550964281225646
1103	66-67	-0.308790720424291
1103	68-69	-0.34967503227173324
1103	70-71	-0.40306825706939975
1103	72-73	-0.3791972770687764
1103	74-75	-0.3794113116966287
1103	76-77	-0.382411799784073
1103	78-79	-0.38557391633416316
1103	80-81	-0.4348752836265959
1103	82-83	-0.45255537731572915
1103	84-85	-0.5695643603933327
1103	86-87	-0.5377493974685166
1103	88-89	-0.5783104537859707
1103	90-91	-0.55147915676012
1103	92-93	-0.5823520236293938
1103	94-95	-0.5575870021000142
1103	96-97	-0.533086221121831
1103	98-99	-0.6579216732178388
1103	100	-0.6535978289706783
1104	1	-0.20403903144564595
1104	2	-0.1749022376265188
1104	3	-0.1691545727726549
1104	4	-0.09132643700223753
1104	5	-0.0412578333858562
1104	6	-0.09425556126163315
1104	7	-0.08530765123172301
1104	8	-0.071280450867512
1104	9	-1.0116527261061492E-4
1104	10-11	-0.05125851694980099
1104	12-13	-0.06977364398095887
1104	14-15	-0.08653183564209144
1104	16-17	-0.12053001519871742
1104	18-19	-0.144077785123379
1104	20-21	-0.1718561141379027
1104	22-23	-0.05481370268583419
1104	24-25	-0.13942444772143858
1104	26-27	-0.12772133945012598
1104	28-29	-0.054492914626322886
1104	30-31	-0.11838986005572139
1104	32-33	-0.12140360465259192
1104	34-35	-0.06140938151202846
1104	36-37	-0.16981267503456365
1104	38-39	-0.0800260277414111
1104	40-41	-0.10500440921292409
1104	42-43	-0.17473147635610076
1104	44-45	-0.24163537119917322
1104	46-47	-0.2848467567281219
1104	48-49	-0.31023150062119953
1104	50-51	-0.3926002644703743
1104	52-53	-0.454634570882952
1104	54-55	-0.30300398038701815
1104	56-57	-0.32918652854003483
1104	58-59	-0.36364308572252213
1104	60-61	-0.4503577806389245
1104	62-63	-0.2911078744852631
1104	64-65	-0.27698272423798187
1104	66-67	-0.22470956106308648
1104	68-69	-0.20305294596830947
1104	70-71	-0.20803824166902274
1104	72-73	-0.18064395759372331
1104	74-75	-0.13112645508317655
1104	76-77	-0.13765203517997548
1104	78-79	-0.1887018365343458
1104	80-81	-0.12938867530202458
1104	82-83	-0.2338498885082423
1104	84-85	-0.20018961634735888
1104	86-87	-0.10088785217981666
1104	88-89	-0.10627138312223394
1104	90-91	-0.1983891452449278
1104	92-93	-0.25506789908579464
1104	94-95	-0.2016019220573071
1104	96-97	-0.22846850435195876
1104	98-99	-0.24931754153323382
1104	100	-0.281713942097241
1105	1	-0.17208853265381308
1105	2	-0.20202400961951028
1105	3	-0.15525702642044337
1105	4	-0.003835324768047599
1105	5	-0.161420779701416
1105	6	-0.1114939701854567
1105	7	-0.0645410817431582
1105	8	-0.16668923755293008
1105	9	-0.1916728084739887
1105	10-11	-0.17245326246239046
1105	12-13	-0.03500912066566286
1105	14-15	-0.1799040137681871
1105	16-17	-0.17968897080935875
1105	18-19	-0.17249079371242715
1105	20-21	-0.1617824852222398
1105	22-23	-0.18383351680947158
1105	24-25	-0.25142710491989106
1105	26-27	-0.20122708919585364
1105	28-29	-0.18214911767051234
1105	30-31	-0.1174342847360279
1105	32-33	-0.10487298467772632
1105	34-35	-0.15665160110079768
1105	36-37	-0.17106522669056545
1105	38-39	-0.16025691850498447
1105	40-41	-0.22821666323848433
1105	42-43	-0.2484273303325466
1105	44-45	-0.1881511569026486
1105	46-47	-0.2899002176109775
1105	48-49	-0.3992573456579436
1105	50-51	-0.34339378471206317
1105	52-53	-0.3488555903311763
1105	54-55	-0.43494209059493016
1105	56-57	-0.4127566804097995
1105	58-59	-0.3604785606558991
1105	60-61	-0.49161433625233997
1105	62-63	-0.33686526787079174
1105	64-65	-0.3090092118296681
1105	66-67	-0.3254399585899961
1105	68-69	-0.2985108197728863
1105	70-71	-0.26843918514078524
1105	72-73	-0.27834062129459625
1105	74-75	-0.25749494769370784
1105	76-77	-0.27870624490218887
1105	78-79	-0.2650376401501404
1105	80-81	-0.2955680704012025
1105	82-83	-0.24093540332399144
1105	84-85	-0.36436954513121833
1105	86-87	-0.3810238539130424
1105	88-89	-0.3582655969248023
1105	90-91	-0.4312400437302095
1105	92-93	-0.39925733734197166
1105	94-95	-0.46785636270789865
1105	96-97	-0.4708611542309775
1105	98-99	-0.5407106110549655
1105	100	-0.5620874308685124
1106	1	-0.16840855623217976
1106	2	-0.18120532092155983
1106	3	-0.1533510651196579
1106	4	-0.05128912993407653
1106	5	-0.09079749593899322
1106	6	-0.06005829934610318
1106	7	-0.0032389034557667173
1106	8	-0.09163043679910743
1106	9	-0.06663806020367957
1106	10-11	-0.06819951606188113
1106	12-13	-0.07396437695779667
1106	14-15	-0.11836666629061199
1106	16-17	-0.06627171583448188
1106	18-19	-0.13579140555822988
1106	20-21	-0.10798084545904629
1106	22-23	-0.08044816005615019
1106	24-25	-0.04941639002190357
1106	26-27	-0.1474599651552282
1106	28-29	-0.1618811443091559
1106	30-31	-0.08182959041736382
1106	32-33	-0.1733836497118375
1106	34-35	-0.08436452984765452
1106	36-37	-0.15018572323439372
1106	38-39	-0.14761076083919278
1106	40-41	-0.12580603387296208
1106	42-43	-0.08735113489479573
1106	44-45	-0.027093241126877388
1106	46-47	-0.05907680918041791
1106	48-49	-0.06356353058791342
1106	50-51	-0.09740327423848072
1106	52-53	-0.10808408542931858
1106	54-55	-0.10377872837062085
1106	56-57	-0.09046493001419265
1106	58-59	-0.23284118032849221
1106	60-61	-0.23038226617659774
1106	62-63	-0.1174248036727974
1106	64-65	-0.13317908826591918
1106	66-67	-0.07665580275267558
1106	68-69	-0.1549452861558791
1106	70-71	-0.0742443576001719
1106	72-73	-0.11283946512359222
1106	74-75	-0.11932027947732138
1106	76-77	-0.0921195620686106
1106	78-79	-0.0808569385459883
1106	80-81	-0.0934233657218968
1106	82-83	-0.10956664647686765
1106	84-85	-0.11764422607148717
1106	86-87	-0.10605906446008007
1106	88-89	-0.14949539564331715
1106	90-91	-0.11387982761471349
1106	92-93	-0.10588778932514842
1106	94-95	-0.16789923041788413
1106	96-97	-0.14618829816754442
1106	98-99	-0.14986663300138403
1106	100	-0.1940674077467861
1107	1	-0.34801216065513785
1107	2	-0.3956734758219902
1107	3	-0.3956432782994135
1107	4	-0.32359776347870906
1107	5	-0.2634835649862026
1107	6	-0.3310578400791968
1107	7	-0.3222284147090164
1107	8	-0.31113827617952694
1107	9	-0.32027381286885515
1107	10-11	-0.33663208478552775
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2216	50-51	0.29388705647315305
2216	52-53	0.28388746388596786
2216	54-55	0.32134032508854204
2216	56-57	0.36929824866686545
2216	58-59	0.40998279003785143
2216	60-61	0.4444045461214614
2216	62-63	0.37434975441483687
2216	64-65	0.32342547301900026
2216	66-67	0.3066282613169662
2216	68-69	0.28415960691848596
2216	70-71	0.25346612722619
2216	72-73	0.29098947987090895
2216	74-75	0.3202117614446678
2216	76-77	0.3346757169358128
2216	78-79	0.3744366140240487
2216	80-81	0.37208609436520845
2216	82-83	0.32398155270376705
2216	84-85	0.3265423983285025
2216	86-87	0.3175383877222657
2216	88-89	0.3404904808323579
2216	90-91	0.35029870283949194
2216	92-93	0.35381860796372777
2216	94-95	0.3136212948583257
2216	96-97	0.3097859761125079
2216	98-99	0.3168289348066651
2216	100	0.34781955220096705
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99738.0
3	20982.0
4	15179.0
5	13060.0
6	8803.0
7	7076.0
8	7807.0
9	8208.0
10	8332.0
11	9038.0
12	10479.0
13	11661.0
14	12956.0
15	13336.0
16	13402.0
17	12858.0
18	12374.0
19	12060.0
20	11431.0
21	12319.0
22	14608.0
23	21694.0
24	34265.0
25	43413.0
26	46040.0
27	36310.0
28	37142.0
29	45164.0
30	53049.0
31	61914.0
32	80273.0
33	107161.0
34	153129.0
35	243855.0
36	414575.0
37	740698.0
38	1049399.0
39	508046.0
40	1816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4769062196586	21.263974201857486	13.93925855299776	22.319861025486148
2	15.017468266855419	22.687707122131116	39.58323246047739	22.711592150536077
3	17.583917746567472	28.76663533987077	30.174269343048383	23.475177570513374
4	12.08902318003111	19.288255836939094	38.18066007879094	30.442060904238854
5	12.33831136379862	40.80394867501314	33.977042447807065	12.88069751338117
6	28.03606305499197	39.828516473255036	18.646408667580427	13.489011804172565
7	25.32370075231578	35.49236899803686	22.559349813248847	16.62458043639851
8	24.209128816918792	37.61381726160424	20.495870653278235	17.681183268198733
9	25.228604742593262	18.932379266854326	20.86298274747512	34.97603324307729
10-11	22.698121151078745	29.440494307551663	28.478860066665497	19.382524474704102
12-13	23.382837971130993	27.690362303735938	28.06628471865535	20.860515006477723
14-15	21.438811754597765	28.975698833685637	25.587009325428582	23.998480086288016
16-17	20.714510191002528	31.880576890737288	26.34030809175611	21.064604826504073
18-19	20.8492956548992	30.8344804596251	27.97268189691347	20.34354198856223
20-21	22.520382175213214	29.628539944724995	27.308019463563916	20.54305841649788
22-23	22.233221820593517	29.71648088482149	27.1956073081049	20.854689986480093
24-25	21.681353210708952	29.883918436853886	27.30152595862502	21.13320239381214
26-27	21.416376093552884	29.886412498253073	27.338943703929218	21.358267704264826
28-29	21.269391918939316	29.82330993398074	27.62232300481447	21.28497514226548
30-31	21.482421144495696	29.976985413290112	27.38819349208328	21.152399950130906
32-33	21.31524532534054	29.933952739892877	27.554117038966364	21.196684895800217
34-35	21.61350300226847	29.658403135264898	27.452617217128267	21.27547664533837
36-37	21.661039391755583	29.730501741795358	27.23770207693823	21.370756789510825
38-39	21.497334043822768	29.76045168775735	27.377872576196626	21.364341692223256
40-41	21.457238418525375	29.657897873278372	27.518808972055453	21.366054736140804
42-43	21.472286703095676	29.686143582195356	27.52648488068105	21.315084834027914
44-45	21.495959511575336	29.65131227034178	27.447528512843405	21.40519970523948
46-47	21.48123628565729	29.675955016955914	27.42470825852783	21.418100438858968
48-49	21.506562972429595	29.590137618253507	27.33000638997553	21.573293019341367
50-51	21.42121629491523	29.623744348485037	27.336295109727573	21.61874424687216
52-53	21.48357151922896	29.555493826898825	27.456766598102973	21.504168055769238
54-55	21.52808133661921	29.682065941749453	27.349071652299067	21.44078106933227
56-57	21.4376724519254	29.544963792887636	27.30878001903984	21.708583736147123
58-59	21.57164765288947	29.372769777445296	27.44008478274422	21.615497786921015
60-61	21.659263163944832	29.62299175650964	27.206722361354352	21.51102271819117
62-63	21.39306831824251	29.61439543821987	27.42348932796272	21.569046915574905
64-65	21.62739310965936	29.445705399327636	27.384158444972094	21.542743046040908
66-67	21.415204178226134	29.513802356080504	27.28562037390152	21.785373091791843
68-69	21.671573584822863	29.57809479926516	27.214683154709547	21.535648461202435
70-71	21.625389461207465	29.44715052085859	27.34340863903589	21.584051378898057
72-73	21.592129481596167	29.603027237533556	27.33918112252371	21.465662158346564
74-75	21.58020774675147	29.525266222197345	27.33436465774495	21.560161373306233
76-77	21.616708513002038	29.443026709967928	27.20991725110033	21.730347525929705
78-79	21.67339250072619	29.48414909784063	27.296838047650574	21.54562035378261
80-81	21.457763612276075	29.524776897088522	27.379837117232853	21.63762237340255
82-83	21.481419049915505	29.53130637802448	27.44194536242397	21.545329209636048
84-85	21.53987215734113	29.432101603843357	27.29757954756845	21.730446691247064
86-87	21.72620145961522	29.41593697542273	27.20206504533744	21.655796519624612
88-89	21.584936684272172	29.334757832271357	27.297091812854852	21.78321367060162
90-91	21.616156074985412	29.30153751016113	27.189493264977383	21.892813149876073
92-93	21.537888844230928	29.43765626823233	27.258365804546404	21.766089082990337
94-95	21.485198615273703	29.33026475179932	27.390568337918296	21.793968295008685
96-97	21.620692707287976	29.40564004303397	27.12193498280262	21.851732266875437
98-99	21.43601365893725	29.479272030359954	27.194857289689132	21.889857021013665
100	21.472378563181557	29.473480777335286	27.18414208089055	21.869998578592607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22918.0
1	17092.5
2	9900.0
3	7250.5
4	4995.5
5	3609.0
6	2964.0
7	2739.0
8	2874.5
9	2902.5
10	2452.5
11	2044.0
12	1964.0
13	1954.5
14	1990.0
15	2074.0
16	2273.5
17	2712.0
18	3386.5
19	4190.0
20	5586.5
21	7934.0
22	11551.5
23	16406.5
24	22417.0
25	29176.5
26	37566.5
27	47477.5
28	59830.0
29	72612.5
30	79994.5
31	86539.0
32	94301.5
33	101794.0
34	108572.5
35	113449.5
36	116945.5
37	120763.0
38	123466.5
39	124188.0
40	125476.5
41	125141.5
42	123968.5
43	123374.5
44	123056.0
45	123312.0
46	124789.5
47	123646.0
48	119278.5
49	117773.5
50	117925.0
51	115080.5
52	109945.0
53	105344.5
54	102414.5
55	99898.0
56	92164.5
57	82012.5
58	75230.5
59	69904.5
60	62572.0
61	53830.0
62	46503.0
63	38506.0
64	30357.0
65	24636.5
66	19944.0
67	15336.0
68	11769.0
69	9058.5
70	6775.5
71	5225.5
72	4123.0
73	3221.5
74	2625.0
75	2032.5
76	1435.0
77	1086.0
78	826.5
79	608.0
80	434.0
81	306.5
82	232.0
83	161.5
84	128.0
85	113.0
86	96.0
87	80.5
88	71.0
89	63.0
90	55.5
91	43.5
92	36.5
93	31.5
94	26.5
95	30.5
96	26.5
97	17.0
98	16.0
99	20.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17839123989386219
2	0.17330858445554545
3	0.18673775740286272
4	0.17684651128025614
5	0.19054974898160026
6	0.07770981525544081
7	0.08055012270626488
8	0.06789331406575064
9	0.06151507979021589
10-11	0.07547992475676754
12-13	0.07429646331892417
14-15	0.07215377524198673
16-17	0.0681300063533193
18-19	0.07232818008545837
20-21	0.07415943094191073
22-23	0.07219114770844492
24-25	0.07206657282025089
26-27	0.07577890448843322
28-29	0.07291368205997036
30-31	0.07848217956224385
32-33	0.0756667870890586
34-35	0.07514357255864362
36-37	0.08520922352472189
38-39	0.08144706190126194
40-41	0.07633949148530639
42-43	0.07976530091064243
44-45	0.09006764416428936
46-47	0.0809736773261246
48-49	0.08626811007437121
50-51	0.08300424800368741
52-53	0.08139723194598433
54-55	0.08353992002292178
56-57	0.07753541041196915
58-59	0.08471092397194574
60-61	0.08155917930063658
62-63	0.07312545936990021
64-65	0.07526814744683766
66-67	0.07595330933190488
68-69	0.0743961232294794
70-71	0.07027269443025674
72-73	0.06808017639804168
74-75	0.07348672654566293
76-77	0.0783202322075916
78-79	0.07462035802822867
80-81	0.08174604163292763
82-83	0.08304162047014563
84-85	0.0849102437930562
86-87	0.0816712967000112
88-89	0.08349009006764416
90-91	0.08147197687890075
92-93	0.07270190475004049
94-95	0.07209148779788971
96-97	0.07138141093518369
98-99	0.08061241015036189
100	0.0881990208413788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4013650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.14284081578266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67501418092623	38.09013869085928
2	15.472512337622108	16.4450652035697
3	5.595891375452384	8.921446937642315
4	2.554729485632015	5.430623295293141
5	1.3499564384709266	3.587026005895068
6	0.7946009667919559	2.5336411614175156
7	0.5367642921820912	1.9967625534520388
8	0.35463835085902407	1.5077191541498238
9	0.26261407653276886	1.2560452258648234
>10	1.2761498137387763	12.03133965481928
>50	0.08332669550226195	3.083105127091909
>100	0.042347697546589645	3.831313371951984
>500	0.001172813521784796	0.43020340749078834
>1k	2.3456268417689969E-4	0.23813761439237754
>5k	0.0	0.0
>10k+	4.691253683537985E-5	0.6174325961098843
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24766	0.6170443362027083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	2.4914977638807568E-5	0.0	0.0	0.0
14-15	0.0	2.4914977638807568E-5	0.0	0.0	0.0
16-17	0.0	2.4914977638807568E-5	0.0	1.2457488819403784E-5	0.0
18-19	0.0	2.4914977638807568E-5	0.0	2.4914977638807568E-5	0.0
20-21	0.0	2.4914977638807568E-5	0.0	3.737246645821135E-5	0.0
22-23	0.0	2.4914977638807568E-5	0.0	1.494898658328454E-4	0.0
24-25	0.0	2.4914977638807568E-5	0.0	4.235546198597287E-4	0.0
26-27	0.0	2.4914977638807568E-5	0.0	9.467691502746876E-4	0.0
28-29	0.0	2.4914977638807568E-5	0.0	0.0025662426967971797	0.0
30-31	0.0	2.4914977638807568E-5	0.0	0.0064903516749093716	0.0
32-33	0.0	2.4914977638807568E-5	0.0	0.013018075816276954	0.0
34-35	0.0	2.4914977638807568E-5	0.0	0.024142613332004535	0.0
36-37	0.0	2.4914977638807568E-5	0.0	0.04176996001146089	0.0
38-39	0.0	2.4914977638807568E-5	0.0	0.06476648437208027	0.0
40-41	0.0	2.4914977638807568E-5	0.0	0.08934510981276395	0.0
42-43	0.0	2.4914977638807568E-5	0.0	0.11475838700434766	0.0
44-45	0.0	2.4914977638807568E-5	0.0	0.1461263438516064	0.0
46-47	0.0	2.4914977638807568E-5	0.0	0.18652598009293286	0.0
48-49	0.0	2.4914977638807568E-5	0.0	0.22984066871799982	0.0
50-51	0.0	2.4914977638807568E-5	0.0	0.2756593125957669	0.0
52-53	0.0	2.4914977638807568E-5	0.0	0.3198335679493728	0.0
54-55	0.0	2.4914977638807568E-5	0.0	0.36775752743761914	0.0
56-57	0.0	2.4914977638807568E-5	0.0	0.4316769025699799	0.0
58-59	0.0	4.9829955277615136E-5	0.0	0.5059110784448071	0.0
60-61	0.0	4.9829955277615136E-5	0.0	0.5853275696685062	0.0
62-63	2.4914977638807568E-5	4.9829955277615136E-5	0.0	0.6672604736337249	0.0
64-65	2.4914977638807568E-5	4.9829955277615136E-5	0.0	0.7522330048708781	0.0
66-67	2.4914977638807568E-5	4.9829955277615136E-5	0.0	0.8536120488831861	0.0
68-69	2.4914977638807568E-5	4.9829955277615136E-5	0.0	0.973066909172449	0.0
70-71	2.4914977638807568E-5	4.9829955277615136E-5	0.0	1.1041321490413962	0.0
72-73	2.4914977638807568E-5	4.9829955277615136E-5	0.0	1.2433819590646917	0.0
74-75	2.4914977638807568E-5	4.9829955277615136E-5	0.0	1.384811829631383	0.0
76-77	2.4914977638807568E-5	4.9829955277615136E-5	0.0	1.5428350753055198	0.0
78-79	2.4914977638807568E-5	4.9829955277615136E-5	0.0	1.7285388611363721	0.0
80-81	2.4914977638807568E-5	4.9829955277615136E-5	0.0	1.9389583047849215	0.0
82-83	2.4914977638807568E-5	4.9829955277615136E-5	0.0	2.1638658079304376	0.0
84-85	2.4914977638807568E-5	4.9829955277615136E-5	0.0	2.390081347401991	0.0
86-87	2.4914977638807568E-5	4.9829955277615136E-5	0.0	2.633189241712656	0.0
88	2.4914977638807568E-5	4.9829955277615136E-5	0.0	2.8354739451621342	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5750	0.0	48.44553	1
GTATCAA	9990	0.0	37.068844	1
ATCAACG	11560	0.0	31.340477	3
TCAACGC	11530	0.0	31.25489	4
TATCAAC	12895	0.0	28.679655	2
CAACGCA	12655	0.0	28.364878	5
GTACATG	23220	0.0	27.681417	1
AACGCAG	13150	0.0	27.1964	6
TACATGG	23135	0.0	26.703485	2
ACATGGG	23775	0.0	25.371155	3
CATGGGT	10185	0.0	25.358866	4
ACGCAGA	14865	0.0	23.774174	7
CGCAGAG	14830	0.0	23.576475	8
TACCTGG	3170	0.0	21.670368	2
ATGGGTA	4095	0.0	21.598442	5
GTGGTAT	2720	0.0	21.278257	1
GCAGAGT	16845	0.0	20.310139	9
CATGGGG	10885	0.0	19.66534	4
ATGGGTG	6090	0.0	19.621622	5
TGGTATC	3020	0.0	18.384329	2
>>END_MODULE