FastQCFastQC Report
Wed 25 May 2016
SRR2431422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013650
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357510.8907353655650094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294310.7332727068877456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182850.4555703661255964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59140.14734717775590797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.1448556799920272No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48360.12048883186127339No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.10287394267063646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116750.063.7236981
GTATCAA232300.045.630591
GTGGTAT37100.039.1684071
ATCAACG300800.034.8766863
TATCAAC303050.034.6505052
TCAACGC303350.034.5977134
TGGTATC41100.034.5541532
CAACGCA315400.033.2311675
AACGCAG323100.032.510336
ACGCAGA361400.028.8569167
CGCAGAG363900.028.5682628
GCAGAGT388550.026.5381419
GTACATG221300.026.010661
CATGGGT98050.025.9445954
TACATGG223750.025.0943782
ACATGGG225550.024.0797843
GAGTACT257750.023.82272712-13
CAGAGTA382400.022.66948110-11
AGAGTAC370350.021.96673210-11
GTACTTT282200.021.5588614-15
Produced by FastQC (version 0.11.5)