##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431422_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4013650
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65220086454973	34.0	31.0	34.0	31.0	34.0
2	32.78648985337536	34.0	31.0	34.0	31.0	34.0
3	32.872423355300036	34.0	31.0	34.0	31.0	34.0
4	36.33369476660895	37.0	37.0	37.0	35.0	37.0
5	36.23914915351364	37.0	37.0	37.0	35.0	37.0
6	36.280049331655725	37.0	37.0	37.0	35.0	37.0
7	36.23724664582114	37.0	37.0	37.0	35.0	37.0
8	36.257285762336025	37.0	37.0	37.0	35.0	37.0
9	38.057410586374	39.0	39.0	39.0	35.0	39.0
10-11	38.035159019844784	39.0	39.0	39.0	35.0	39.0
12-13	38.01317865284716	39.0	39.0	39.0	35.0	39.0
14-15	39.480178889539445	41.0	39.0	41.0	36.0	41.0
16-17	39.50402962390841	41.0	39.0	41.0	36.5	41.0
18-19	39.57472923648051	41.0	40.0	41.0	37.0	41.0
20-21	39.571465748134486	41.0	40.0	41.0	37.0	41.0
22-23	39.51427839996013	41.0	40.0	41.0	36.0	41.0
24-25	39.46438927161063	41.0	39.0	41.0	36.0	41.0
26-27	39.347781196666375	41.0	39.0	41.0	36.0	41.0
28-29	39.26932729560375	41.0	39.0	41.0	35.5	41.0
30-31	39.08701369078021	41.0	39.0	41.0	35.0	41.0
32-33	38.86455869345858	41.0	38.0	41.0	35.0	41.0
34-35	38.736480883983404	41.0	38.0	41.0	35.0	41.0
36-37	38.60337186849875	41.0	38.0	41.0	35.0	41.0
38-39	38.465471079939704	41.0	38.0	41.0	35.0	41.0
40-41	38.32607975284343	40.0	38.0	41.0	35.0	41.0
42-43	38.149549288054516	40.0	38.0	41.0	34.0	41.0
44-45	37.9027213384326	40.0	38.0	41.0	33.5	41.0
46-47	37.534551468115055	40.0	37.5	41.0	33.0	41.0
48-49	37.198644251491785	40.0	37.0	41.0	32.0	41.0
50-51	37.02261819042518	40.0	37.0	41.0	32.0	41.0
52-53	36.95078743786827	40.0	36.5	41.0	32.0	41.0
54-55	36.79747760766385	40.0	36.0	41.0	31.5	41.0
56-57	36.628967025027094	40.0	35.5	41.0	31.0	41.0
58-59	36.43537328865247	39.5	35.0	41.0	31.0	41.0
60-61	36.25943829182913	39.0	35.0	41.0	31.0	41.0
62-63	36.08153413974811	39.0	35.0	41.0	31.0	41.0
64-65	35.83434392635132	39.0	35.0	41.0	31.0	41.0
66-67	35.55818743537678	38.0	35.0	41.0	30.0	41.0
68-69	35.25799957644538	37.0	35.0	40.5	30.0	41.0
70-71	34.95633089581802	37.0	35.0	40.0	29.5	41.0
72-73	34.636146774133266	36.0	35.0	39.0	29.0	41.0
74-75	34.30336999987543	36.0	35.0	39.0	29.0	41.0
76-77	33.982345371420024	35.0	35.0	38.5	29.0	40.5
78-79	33.678377287506386	35.0	34.0	37.0	29.0	39.5
80-81	33.4321600039864	35.0	34.0	37.0	29.0	39.0
82-83	33.250000996599105	35.0	34.0	36.5	29.0	39.0
84-85	33.00628343278562	35.0	34.0	36.0	29.0	38.0
86-87	32.78208251840594	35.0	34.0	36.0	29.0	37.0
88-89	32.56584530290384	35.0	34.0	36.0	29.0	37.0
90-91	32.395853898571126	35.0	34.0	35.0	29.0	36.5
92-93	32.24709703138041	35.0	34.0	35.0	29.0	36.0
94-95	32.117624979756584	35.0	34.0	35.0	27.5	36.0
96-97	31.984519203219016	35.0	34.0	35.0	27.0	36.0
98-99	31.874566043376976	35.0	34.0	35.0	27.0	36.0
100	31.77759670125696	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.005677269681761743
1101	2	0.027890529961823063
1101	3	0.021968141277234565
1101	4	0.06828086446865456
1101	5	0.04272249586216503
1101	6	0.06697152362408332
1101	7	0.04794503305371478
1101	8	0.06537183117393397
1101	9	0.05680640133316217
1101	10-11	0.034068258423438635
1101	12-13	0.03182082684297427
1101	14-15	0.010725614411050799
1101	16-17	-0.007832927204930229
1101	18-19	0.011672385492310866
1101	20-21	0.047825406656379243
1101	22-23	0.04120686667570084
1101	24-25	0.07293956366865473
1101	26-27	0.10437071273024401
1101	28-29	0.07334761272724677
1101	30-31	0.0440130202536011
1101	32-33	0.07017559413164776
1101	34-35	0.038045607923763214
1101	36-37	0.044378960665724776
1101	38-39	0.016431712863720804
1101	40-41	-0.042276481288503476
1101	42-43	-0.07457889682385144
1101	44-45	-0.09048922795042813
1101	46-47	-0.04669395186409275
1101	48-49	-0.03978257547694852
1101	50-51	-0.1325921016077345
1101	52-53	-0.10906804585301444
1101	54-55	-0.0900122390167013
1101	56-57	-0.058945984741882285
1101	58-59	-0.1182069873032745
1101	60-61	-0.15377042987798006
1101	62-63	-0.14251093732647035
1101	64-65	-0.13254069166214322
1101	66-67	-0.1301570409562487
1101	68-69	-0.09368975404706248
1101	70-71	-0.17334428108600974
1101	72-73	-0.23522526777085773
1101	74-75	-0.26923611602448716
1101	76-77	-0.23477057677566648
1101	78-79	-0.12735385695816603
1101	80-81	-0.10486925035114325
1101	82-83	-0.2023865224144501
1101	84-85	-0.1605140498563955
1101	86-87	-0.15396338403667187
1101	88-89	-0.120429389401572
1101	90-91	-0.1464891168929512
1101	92-93	-0.15003014226209643
1101	94-95	-0.20877424377732368
1101	96-97	-0.19750418957902838
1101	98-99	-0.19024071395610065
1101	100	-0.15248846062326038
1102	1	-0.05396044056152505
1102	2	0.0016718098531924852
1102	3	0.005769426056588145
1102	4	0.021608787431304677
1102	5	-0.014778416698867147
1102	6	-0.033723197522213866
1102	7	-0.014074540199565888
1102	8	0.011316790240087471
1102	9	-0.018655189839016373
1102	10-11	0.017110458345875657
1102	12-13	-0.004557174223279503
1102	14-15	-0.03192756080478887
1102	16-17	-0.054126839435816976
1102	18-19	-0.03739287830504168
1102	20-21	-0.03145147077898969
1102	22-23	0.018629701072093496
1102	24-25	0.011199390322019553
1102	26-27	-0.002175117211010047
1102	28-29	-0.0436654193951469
1102	30-31	-0.04186838895862621
1102	32-33	-0.026503222656373282
1102	34-35	-0.054264391056491945
1102	36-37	-0.1396477212381484
1102	38-39	-0.13527707734557737
1102	40-41	-0.19338226667110092
1102	42-43	-0.15193340116938714
1102	44-45	-0.20972410698976773
1102	46-47	-0.27009364560207416
1102	48-49	-0.23679423784946607
1102	50-51	-0.24984823102644782
1102	52-53	-0.2531457488200317
1102	54-55	-0.2253586949009474
1102	56-57	-0.18783932615887977
1102	58-59	-0.18792437920263438
1102	60-61	-0.21892768103828075
1102	62-63	-0.19154631732366312
1102	64-65	-0.25211564653064755
1102	66-67	-0.22758610159849724
1102	68-69	-0.2613855901285831
1102	70-71	-0.28080573007548537
1102	72-73	-0.2769672444398168
1102	74-75	-0.26350154496692824
1102	76-77	-0.31219659635522135
1102	78-79	-0.2566570636261716
1102	80-81	-0.2609892164598193
1102	82-83	-0.2558648998651947
1102	84-85	-0.24241821895837035
1102	86-87	-0.2768249816518775
1102	88-89	-0.31174211722391476
1102	90-91	-0.3231632939712057
1102	92-93	-0.2949617892004035
1102	94-95	-0.3392906055666529
1102	96-97	-0.3415754737358796
1102	98-99	-0.33358079874737356
1102	100	-0.30639802467463184
1103	1	-0.06824139721475575
1103	2	-0.06946755863880583
1103	3	-0.08966976004551697
1103	4	0.004092251213045017
1103	5	-1.0834083499844382E-4
1103	6	-0.024150619269491358
1103	7	-0.07572536796470075
1103	8	-0.027934786234986575
1103	9	-0.014326912718544804
1103	10-11	0.03543432405049174
1103	12-13	0.0019797019523863923
1103	14-15	-0.05918557422479154
1103	16-17	-0.010934921634763839
1103	18-19	-0.07152508644031741
1103	20-21	-0.05457241117478162
1103	22-23	-0.0926640256299649
1103	24-25	-0.038886196130775375
1103	26-27	-0.05319945226146672
1103	28-29	-0.03170684950590896
1103	30-31	-0.02062473603881898
1103	32-33	-0.015742824158515134
1103	34-35	0.02259913134240321
1103	36-37	0.010141678624862038
1103	38-39	-0.03054928792438716
1103	40-41	-0.0538446360451772
1103	42-43	-0.017223359395096338
1103	44-45	-0.0935085975033445
1103	46-47	-0.11524385346686472
1103	48-49	-0.14578769418737636
1103	50-51	-0.19441992516244966
1103	52-53	-0.12670514938950106
1103	54-55	-0.08373088173352983
1103	56-57	-0.08835700630612564
1103	58-59	-0.09164841303024929
1103	60-61	-0.1303934434911227
1103	62-63	-0.1706825260046898
1103	64-65	-0.1718136411286295
1103	66-67	-0.08705134550535831
1103	68-69	-0.11463830376170847
1103	70-71	-0.12289882951306197
1103	72-73	-0.19537136073864758
1103	74-75	-0.11114300858484683
1103	76-77	-0.12119541851162552
1103	78-79	-0.1453857768909117
1103	80-81	-0.12330851555629607
1103	82-83	-0.09187810151636455
1103	84-85	-0.10042261803074126
1103	86-87	-0.06454376458717093
1103	88-89	-0.08772449792523673
1103	90-91	-0.09051197983701798
1103	92-93	-0.10536522145474692
1103	94-95	-0.12514889355118441
1103	96-97	-0.07885728550464677
1103	98-99	-0.08489708705343091
1103	100	-0.09332167180378548
1104	1	-0.17971384992411288
1104	2	-0.13219152539358703
1104	3	-0.13552760261089247
1104	4	-0.05725160876448854
1104	5	-0.054981486422676085
1104	6	-0.09414611635600778
1104	7	-0.10038331147686819
1104	8	-0.12447253118985913
1104	9	-0.1329050648183312
1104	10-11	-0.13205479256763653
1104	12-13	-0.123847586597293
1104	14-15	-0.14958359619689787
1104	16-17	-0.11762715219143871
1104	18-19	-0.10369543978358564
1104	20-21	-0.058002876741348075
1104	22-23	-0.10001131969363541
1104	24-25	-0.07587838580633388
1104	26-27	-0.13109104996078713
1104	28-29	-0.12051450358274707
1104	30-31	-0.09666957601017856
1104	32-33	-0.04338000322312041
1104	34-35	0.022707614278928645
1104	36-37	0.034872078189820854
1104	38-39	-0.016786392557278873
1104	40-41	0.013872138295383252
1104	42-43	0.019315499443081308
1104	44-45	-0.01003327980747315
1104	46-47	0.09315097364476799
1104	48-49	0.10515769862232105
1104	50-51	-0.02942475392703159
1104	52-53	0.041447469787300406
1104	54-55	0.13490671025459733
1104	56-57	0.06496505231113758
1104	58-59	0.009294400683678816
1104	60-61	0.023439562158479532
1104	62-63	-0.029322197360841074
1104	64-65	0.047310179694925125
1104	66-67	-0.005591611269338159
1104	68-69	-0.021492539079474682
1104	70-71	0.030498858741040635
1104	72-73	-0.02609793001114724
1104	74-75	-0.006676309717477125
1104	76-77	0.011189790941791955
1104	78-79	-0.018132699832989374
1104	80-81	-0.02151075617815934
1104	82-83	-0.004858873798660568
1104	84-85	0.011117463307776632
1104	86-87	-0.017537486880328856
1104	88-89	0.026286598533758365
1104	90-91	0.02042632738755401
1104	92-93	0.02253039462776485
1104	94-95	0.03518039395416395
1104	96-97	0.028101570810754595
1104	98-99	-0.03131840617196602
1104	100	0.053466066647473554
1105	1	-0.184310026312275
1105	2	-0.14089183159083518
1105	3	-0.14831875673883843
1105	4	-0.0721099168336039
1105	5	-0.13751093553532456
1105	6	-0.06107802977426502
1105	7	-0.04626897195838353
1105	8	-0.05792591272231107
1105	9	-0.045227829978465195
1105	10-11	-0.07713748709991819
1105	12-13	-0.057526833524192966
1105	14-15	-0.12176501043544619
1105	16-17	-0.14487848126817227
1105	18-19	-0.21796430279103163
1105	20-21	-0.15394727280391152
1105	22-23	-0.17604434349749454
1105	24-25	-0.16342901672346954
1105	26-27	-0.096115471133146
1105	28-29	-0.12860119875197285
1105	30-31	-0.09966843021308591
1105	32-33	-0.21540241104489866
1105	34-35	-0.16585907739704453
1105	36-37	-0.15112828717937532
1105	38-39	-0.12698034882657794
1105	40-41	-0.12658988183542164
1105	42-43	-0.17570714013572797
1105	44-45	-0.18725918672248554
1105	46-47	-0.17901822244104437
1105	48-49	-0.17518436322650643
1105	50-51	-0.15168063924635078
1105	52-53	-0.13067101126770808
1105	54-55	-0.15457714801706857
1105	56-57	-0.19019356901931417
1105	58-59	-0.17243472416307526
1105	60-61	-0.17147361946230433
1105	62-63	-0.20746359888287458
1105	64-65	-0.2573658407639883
1105	66-67	-0.3211559216984483
1105	68-69	-0.2379408025928882
1105	70-71	-0.2551069848858205
1105	72-73	-0.16817801791602704
1105	74-75	-0.18519089676081535
1105	76-77	-0.23766954694304587
1105	78-79	-0.1911671456499846
1105	80-81	-0.1183721013897241
1105	82-83	-0.17307527415043467
1105	84-85	-0.14244926025058646
1105	86-87	-0.08709594758232697
1105	88-89	-0.04728989484524959
1105	90-91	-0.023962048136169756
1105	92-93	-0.1030697912715155
1105	94-95	-0.07517238654683922
1105	96-97	-0.099061072342991
1105	98-99	-0.051734063731760926
1105	100	-0.04845924447244698
1106	1	-0.18323466917390618
1106	2	-0.15312877569814276
1106	3	-0.1245359158533006
1106	4	-0.033504511003407345
1106	5	-0.0927153624776409
1106	6	-0.12675359957120236
1106	7	-0.09547934411764203
1106	8	-0.04463723268979436
1106	9	-0.09345741441480726
1106	10-11	-0.11311243970668983
1106	12-13	-0.09030035068280995
1106	14-15	-0.16569670386275703
1106	16-17	-0.18019285734708745
1106	18-19	-0.18993670453228617
1106	20-21	-0.18541918501102117
1106	22-23	-0.1485418098546063
1106	24-25	-0.09758477229702578
1106	26-27	-0.14482678174234564
1106	28-29	-0.17758945258226078
1106	30-31	-0.2544093486317891
1106	32-33	-0.20662569278909615
1106	34-35	-0.2106510283474563
1106	36-37	-0.25361242981925614
1106	38-39	-0.2498238772314778
1106	40-41	-0.3741723436878672
1106	42-43	-0.3785925164230548
1106	44-45	-0.30813147133295615
1106	46-47	-0.3076240020388923
1106	48-49	-0.19974402632374222
1106	50-51	-0.20139967083524368
1106	52-53	-0.28580189111053755
1106	54-55	-0.21471723590489944
1106	56-57	-0.20877030016249165
1106	58-59	-0.20837709189822817
1106	60-61	-0.22116821239748674
1106	62-63	-0.2641329610362604
1106	64-65	-0.2618940386012909
1106	66-67	-0.19472516818682095
1106	68-69	-0.24626350309173972
1106	70-71	-0.192139694290276
1106	72-73	-0.15085741102335248
1106	74-75	-0.10508655695765867
1106	76-77	-0.10235002944494198
1106	78-79	-0.1893759501837735
1106	80-81	-0.1509226968726125
1106	82-83	-0.16982727277849108
1106	84-85	-0.07703975325913603
1106	86-87	-0.1006636708125086
1106	88-89	-0.1441321614601918
1106	90-91	-0.14986865312910425
1106	92-93	-0.07933038748559795
1106	94-95	-0.07337772400518361
1106	96-97	-0.13241804474163033
1106	98-99	-0.11428613680891786
1106	100	-0.09807529264573844
1107	1	-0.238594598781674
1107	2	-0.18242390087955584
1107	3	-0.1739788519121106
1107	4	-0.21365314306460448
1107	5	-0.22659321204891114
1107	6	-0.2223091519381697
1107	7	-0.1767438700479005
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2216	10-11	0.026363483853721448
2216	12-13	0.010484339246971786
2216	14-15	0.022782532352074725
2216	16-17	0.013424552794440103
2216	18-19	-0.039954642392935114
2216	20-21	-0.028403118993914234
2216	22-23	-0.08653824613337235
2216	24-25	-0.075874359565411
2216	26-27	0.007281420553354678
2216	28-29	-0.01363984554834019
2216	30-31	-0.07910594762967094
2216	32-33	-0.027025200701764618
2216	34-35	0.0479590318335994
2216	36-37	0.06577888088409622
2216	38-39	0.08136795741459935
2216	40-41	0.09605597984626968
2216	42-43	0.115899191641617
2216	44-45	0.09599841052289548
2216	46-47	0.07729192852501399
2216	48-49	0.11713724870897124
2216	50-51	0.18228283418089575
2216	52-53	0.18740092813753506
2216	54-55	0.15024139047146434
2216	56-57	0.14953094513429477
2216	58-59	0.17080461822359183
2216	60-61	0.084992627378071
2216	62-63	0.04884864748436257
2216	64-65	0.027949152605103222
2216	66-67	-0.006941673421778205
2216	68-69	-0.027045996751610346
2216	70-71	-0.031053888617428527
2216	72-73	-0.0350997570768925
2216	74-75	0.007844540065164551
2216	76-77	0.014908130384419849
2216	78-79	0.0998528997865975
2216	80-81	0.036925574755116486
2216	82-83	0.04582079804998784
2216	84-85	0.01717462490963584
2216	86-87	0.07671543564080707
2216	88-89	0.09530611563065605
2216	90-91	0.05024805815408939
2216	92-93	0.05163736167743593
2216	94-95	0.009343052392040363
2216	96-97	-0.005023796583728313
2216	98-99	0.04278445199289038
2216	100	0.05485045780144304
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70.0
3	405.0
4	97.0
5	145.0
6	328.0
7	706.0
8	1315.0
9	2203.0
10	3123.0
11	4477.0
12	6614.0
13	9976.0
14	11447.0
15	15274.0
16	20296.0
17	31107.0
18	37067.0
19	19608.0
20	12391.0
21	11313.0
22	11270.0
23	11170.0
24	11763.0
25	13862.0
26	17076.0
27	21748.0
28	27319.0
29	34710.0
30	43626.0
31	54803.0
32	69343.0
33	89901.0
34	126522.0
35	216291.0
36	362864.0
37	746623.0
38	1231408.0
39	728468.0
40	6921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28358487124774	20.441919707861878	14.594522808839491	22.679972612050896
2	14.810715672111478	21.655114780517483	40.145399784626136	23.388769762744904
3	16.76737400993121	28.0702659202065	31.72734769945261	23.43501237040968
4	11.391949970724902	18.401928419269247	38.8808441194424	31.32527749056345
5	11.748087479775556	38.905588016310666	35.026532627192466	14.319791876721306
6	27.849089980441743	39.2996898085284	18.31338058874092	14.537839622288939
7	24.74759881903006	35.13487723144769	22.871451172872572	17.246072776649683
8	25.36269978697694	34.85099597623111	20.598507592839436	19.187796643952513
9	26.382433138132694	15.67915273088223	21.615971305904257	36.32244282508083
10-11	23.43632354589962	27.211079690555977	28.833119479775267	20.519477283769138
12-13	25.05095112927136	25.643304722634014	29.06231235907466	20.24343178901997
14-15	22.364132398191174	26.467454810459305	27.92460727766497	23.243805513684553
16-17	20.00021177730993	29.720466408381398	28.570291380663488	21.709030433645186
18-19	19.94788528933543	27.215603967066354	32.64557960517694	20.190931138421277
20-21	21.438416404021275	26.220385434704074	32.56899580182627	19.772202359448382
22-23	21.09230541737773	26.31152179868267	32.575411125314034	20.020761658625563
24-25	20.664076504945996	26.46540591865407	32.59090418718727	20.27961338921266
26-27	20.452528472850634	26.54480412831419	32.602535150067965	20.400132248767207
28-29	20.324871659723893	26.462295570873017	32.849978193146804	20.36285457625628
30-31	20.581631708577714	26.611483168829686	32.58191225195985	20.22497287063275
32-33	20.44559292963218	26.61276820088519	32.65050842187405	20.29113044760858
34-35	20.700030209692642	26.49584383146106	32.46344782910657	20.34067812973973
36-37	20.75550580806146	26.55737582383906	32.11317015075609	20.573948217343396
38-39	20.65529535754595	26.64647287603928	32.119451067742006	20.578780698672766
40-41	20.640893910870023	26.605872369348155	32.11762179206726	20.635611927714564
42-43	20.715925573100357	26.717613073154862	31.927722775607464	20.63873857813732
44-45	20.850540773832474	26.782988985203353	31.569795695759456	20.796674545204716
46-47	20.993080103766054	26.99479575169098	31.070244396364004	20.941879748178962
48-49	21.140579625469975	27.12897894394457	30.47074310411475	21.259698326470698
50-51	21.1385610032199	27.308065889873184	30.243172245418837	21.31020086148808
52-53	21.131381796446753	27.426654172551835	30.24549141760449	21.196472613396924
54-55	21.194291385690992	27.527666119737088	30.11095847799187	21.167084016580052
56-57	21.059394827291946	27.381193527450037	30.132812100962607	21.426599544295406
58-59	21.146899868784566	27.13235666928317	30.36106854433588	21.359674917596386
60-61	21.218518015599443	27.22148791423172	30.282962573546722	21.277031496622115
62-63	21.00986592625296	27.190047269143108	30.504443379180646	21.295643425423286
64-65	21.121455695366762	27.0628990974906	30.526733318600048	21.288911888542593
66-67	21.06710460700664	27.06726182104433	30.37357767700189	21.492055894947136
68-69	21.347117004572908	27.16807046375383	30.131105495812026	21.35370703586123
70-71	21.267573051235182	27.113029735986437	30.18286275657362	21.43653445620476
72-73	21.234541325222004	27.26130826982808	30.08207378605654	21.422076618893378
74-75	21.236434966070597	27.252837084054626	29.951629797280667	21.55909815259411
76-77	21.250142483504614	27.23720280121013	29.81833742460832	21.69431729067694
78-79	21.282840964385542	27.270361466435688	29.89122296737885	21.555574601799922
80-81	21.0615597256593	27.327557586693818	29.9671431481162	21.643739539530685
82-83	21.077692462167324	27.28230608277768	30.059268575745328	21.580732879309668
84-85	21.09048181658271	27.265271942758652	29.85226238177079	21.79198385888785
86-87	21.173099088244044	27.282429575236435	29.74361504235377	21.800856294165747
88-89	21.054418921248832	27.143958397490138	29.829238260675446	21.972384420585588
90-91	21.035204012352985	27.16425756894363	29.720413443774362	22.08012497492902
92-93	20.911275858705412	27.26527423099553	29.7985269885785	22.02492292172056
94-95	20.889497759444115	27.15501911053214	29.88645300525747	22.069030124766282
96-97	20.97820322651894	27.248850763937867	29.610955828300188	22.161990181243006
98-99	20.82230375256765	27.223166710205714	29.68306687311276	22.27146266411387
100	20.872972074012523	27.17019068701891	29.686631478369414	22.270205760599158
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2134.0
1	1301.0
2	2132.0
3	3918.0
4	2534.0
5	1579.5
6	4110.5
7	10934.5
8	19999.0
9	22609.0
10	14307.5
11	6211.5
12	4758.0
13	4951.5
14	5440.0
15	5880.0
16	6459.5
17	7092.5
18	7731.0
19	8439.5
20	9670.0
21	11750.0
22	14973.0
23	19522.0
24	25017.0
25	30905.0
26	38725.0
27	48240.5
28	59523.0
29	71680.5
30	79347.0
31	85574.5
32	92847.0
33	99682.0
34	106092.0
35	111392.5
36	114788.0
37	118291.5
38	121520.5
39	122380.5
40	123732.0
41	123770.5
42	122337.5
43	122665.0
44	123500.5
45	123288.0
46	123888.0
47	123130.0
48	118900.0
49	116750.5
50	116987.0
51	114512.0
52	108393.5
53	103467.5
54	100225.0
55	96704.5
56	88742.0
57	78507.0
58	72763.0
59	68738.5
60	61575.0
61	52067.0
62	45174.5
63	37389.0
64	28728.5
65	22914.0
66	18030.5
67	13594.0
68	10275.5
69	7656.5
70	5511.0
71	4222.5
72	3343.0
73	2530.5
74	2120.0
75	1581.5
76	865.5
77	638.5
78	520.0
79	268.5
80	120.0
81	56.5
82	27.5
83	21.5
84	13.5
85	5.5
86	3.5
87	3.5
88	1.5
89	0.5
90	2.0
91	2.5
92	1.5
93	1.5
94	0.5
95	1.0
96	1.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005132485393594359
2	0.004086056332764441
3	9.965991055523027E-4
4	0.0
5	0.0447722148169372
6	0.0
7	0.0
8	0.0
9	4.9829955277615136E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.965991055523027E-5
20-21	0.0
22-23	3.737246645821135E-5
24-25	2.4914977638807568E-5
26-27	4.9829955277615136E-5
28-29	8.720242173582649E-5
30-31	2.616072652074795E-4
32-33	0.0013454087924956086
34-35	9.343116614552838E-4
36-37	0.0011834614378433597
38-39	9.965991055523027E-4
40-41	1.494898658328454E-4
42-43	5.107570415955552E-4
44-45	8.720242173582649E-5
46-47	1.494898658328454E-4
48-49	1.619473546522492E-4
50-51	3.3635219812390215E-4
52-53	6.727043962478043E-4
54-55	7.848217956224385E-4
56-57	0.0021177730992986434
58-59	5.356720192343627E-4
60-61	0.0011211739937463407
62-63	9.467691502746876E-4
64-65	0.001569643591244877
66-67	3.3635219812390215E-4
68-69	2.989797316656908E-4
70-71	2.8652224284628707E-4
72-73	1.3703237701344164E-4
74-75	5.481295080537665E-4
76-77	6.851618850672082E-4
78-79	6.727043962478043E-4
80-81	6.851618850672082E-4
82-83	0.0013952387477732238
84-85	0.0011959189266627633
86-87	0.0018935383005493753
88-89	0.0011460889713851483
90-91	0.0011211739937463407
92-93	8.844817061776686E-4
94-95	6.35331929789593E-4
96-97	1.3703237701344164E-4
98-99	2.8652224284628707E-4
100	1.2457488819403784E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4013650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.32722625635063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.16713004006132	32.9432831669212
2	17.221095049701262	16.644955136980656
3	6.23121010418115	9.034113016668618
4	2.8978705093167543	5.601841750614273
5	1.5782813311826287	3.813697949411858
6	0.9338332537696803	2.7077742564378866
7	0.6166452105073048	2.0860526825657035
8	0.43206170755703066	1.6704275118251055
9	0.29119460244447015	1.2665364693265784
>10	1.4708173298175393	12.557761587423308
>50	0.10147680795391588	3.3922251283178433
>100	0.055539756186370984	4.616777828543686
>500	0.001861721924300922	0.6071600710047927
>1k	7.240029235525433E-4	0.6782627942811398
>5k	1.034289890789346E-4	0.2931082962279081
>10k+	1.551434836184019E-4	2.086022353449421
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35751	0.8907353655650094	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29431	0.7332727068877456	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18285	0.4555703661255964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5914	0.14734717775590797	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5814	0.1448556799920272	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4836	0.12048883186127339	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4129	0.10287394267063646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.2457488819403784E-5	0.0
18-19	0.0	0.0	0.0	2.4914977638807568E-5	0.0
20-21	0.0	0.0	0.0	3.737246645821135E-5	0.0
22-23	0.0	0.0	0.0	1.494898658328454E-4	0.0
24-25	0.0	0.0	0.0	3.986396422209211E-4	0.0
26-27	0.0	0.0	0.0	9.093966838164762E-4	0.0
28-29	0.0	2.4914977638807568E-5	0.0	0.002541327719158372	0.0
30-31	0.0	2.4914977638807568E-5	0.0	0.006477894186089968	0.0
32-33	0.0	2.4914977638807568E-5	0.0	0.0131675656821098	0.0
34-35	0.0	2.4914977638807568E-5	0.0	0.024217358264920958	0.0
36-37	0.0	2.4914977638807568E-5	0.0	0.04196927983257135	0.0
38-39	0.0	2.4914977638807568E-5	0.0	0.06519003899194001	0.0
40-41	0.0	2.4914977638807568E-5	0.0	0.0898184943879013	0.0
42-43	0.0	2.4914977638807568E-5	0.0	0.11539371893413726	0.0
44-45	0.0	2.4914977638807568E-5	0.0	0.14668693084847956	0.0
46-47	0.0	2.4914977638807568E-5	0.0	0.1873357168661941	0.0
48-49	0.0	2.4914977638807568E-5	0.0	0.23114870504403723	0.0
50-51	0.0	2.4914977638807568E-5	0.0	0.2775403934074969	0.0
52-53	0.0	2.4914977638807568E-5	0.0	0.32212574589214304	0.0
54-55	0.0	2.4914977638807568E-5	0.0	0.3706974947989984	0.0
56-57	0.0	2.4914977638807568E-5	0.0	0.43537677674934283	0.0
58-59	0.0	2.4914977638807568E-5	0.0	0.5105203493079865	0.0
60-61	0.0	2.4914977638807568E-5	0.0	0.59130716430182	0.0
62-63	0.0	2.4914977638807568E-5	0.0	0.6744858171489791	0.0
64-65	0.0	2.4914977638807568E-5	0.0	0.7604798624693234	0.0
66-67	0.0	2.4914977638807568E-5	0.0	0.8634908375169734	0.0
68-69	0.0	2.4914977638807568E-5	0.0	0.9845527138639394	0.0
70-71	0.0	2.4914977638807568E-5	0.0	1.1172000548129508	0.0
72-73	0.0	2.4914977638807568E-5	0.0	1.2588043302231138	0.0
74-75	0.0	2.4914977638807568E-5	0.0	1.4023768888667423	0.0
76-77	0.0	2.4914977638807568E-5	0.0	1.5631781545476064	0.0
78-79	0.0	2.4914977638807568E-5	0.0	1.752071057516226	0.0
80-81	0.0	2.4914977638807568E-5	0.0	1.9650692013503916	0.0
82-83	0.0	2.4914977638807568E-5	0.0	2.193215651588953	0.0
84-85	0.0	2.4914977638807568E-5	0.0	2.4228071705305645	0.0
86-87	0.0	2.4914977638807568E-5	0.0	2.6707984004584358	0.0
88	0.0	2.4914977638807568E-5	0.0	2.8769822979083877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11675	0.0	63.723698	1
GTATCAA	23230	0.0	45.63059	1
GTGGTAT	3710	0.0	39.168407	1
ATCAACG	30080	0.0	34.876686	3
TATCAAC	30305	0.0	34.650505	2
TCAACGC	30335	0.0	34.597713	4
TGGTATC	4110	0.0	34.554153	2
CAACGCA	31540	0.0	33.231167	5
AACGCAG	32310	0.0	32.51033	6
ACGCAGA	36140	0.0	28.856916	7
CGCAGAG	36390	0.0	28.568262	8
GCAGAGT	38855	0.0	26.538141	9
GTACATG	22130	0.0	26.01066	1
CATGGGT	9805	0.0	25.944595	4
TACATGG	22375	0.0	25.094378	2
ACATGGG	22555	0.0	24.079784	3
GAGTACT	25775	0.0	23.822727	12-13
CAGAGTA	38240	0.0	22.669481	10-11
AGAGTAC	37035	0.0	21.966732	10-11
GTACTTT	28220	0.0	21.55886	14-15
>>END_MODULE