FastQCFastQC Report
Wed 25 May 2016
SRR2431421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478811
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA354571.0192275464231888No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38560.11084246887801608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.046.8914381
GTATCAA97450.038.535181
TCAACGC112700.031.6868534
ATCAACG112700.031.6047233
TATCAAC121850.030.3898962
CAACGCA125350.028.6767735
AACGCAG128950.027.7335666
GTACATG209150.026.8422471
TACATGG209600.025.4117852
ACGCAGA143650.024.666897
CGCAGAG144150.024.2227158
CATGGGT96700.023.744044
ACATGGG215150.023.619053
ATGGGTG53450.021.5664985
GCAGAGT159100.021.3269219
GTGGTAT28050.021.3062571
TACCTGG25400.020.747432
CAGAGTA153550.018.66986510-11
CATGGGG98600.018.371524
ATGGGTA40600.017.7307325
Produced by FastQC (version 0.11.5)