##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431421_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3478811
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.491554442020565	33.0	31.0	34.0	26.0	34.0
2	30.5098788062933	33.0	31.0	34.0	26.0	34.0
3	30.716435874211044	34.0	31.0	34.0	26.0	34.0
4	33.871149079383734	37.0	35.0	37.0	28.0	37.0
5	34.03101002037765	37.0	35.0	37.0	30.0	37.0
6	34.00978236529665	37.0	35.0	37.0	30.0	37.0
7	33.98114413229118	37.0	35.0	37.0	30.0	37.0
8	33.91640850853927	37.0	35.0	37.0	30.0	37.0
9	35.30237457568117	39.0	37.0	39.0	27.0	39.0
10-11	35.3436849544284	39.0	37.0	39.0	27.5	39.0
12-13	35.263510435030824	39.0	37.0	39.0	27.0	39.0
14-15	36.58866995073892	41.0	38.0	41.0	27.0	41.0
16-17	36.608076437610436	40.5	38.0	41.0	27.5	41.0
18-19	36.61010601035814	40.0	38.0	41.0	27.5	41.0
20-21	36.68126609925058	40.5	38.0	41.0	28.0	41.0
22-23	36.656220185574895	40.0	38.0	41.0	28.0	41.0
24-25	36.69970688261018	40.0	38.0	41.0	29.0	41.0
26-27	36.74107762680985	40.0	38.0	41.0	29.5	41.0
28-29	36.715599094058284	40.0	38.0	41.0	29.5	41.0
30-31	36.64520262813933	40.0	37.0	41.0	30.0	41.0
32-33	36.597929148781006	40.0	37.0	41.0	29.5	41.0
34-35	36.5224946109461	40.0	37.0	41.0	29.0	41.0
36-37	36.38256433016913	40.0	37.0	41.0	28.0	41.0
38-39	36.25118984618595	40.0	37.0	41.0	27.5	41.0
40-41	36.17615716979164	40.0	36.5	41.0	27.0	41.0
42-43	35.99619410195035	40.0	36.0	41.0	27.0	41.0
44-45	35.82335961913424	40.0	36.0	41.0	27.0	41.0
46-47	35.68388207925064	40.0	35.0	41.0	26.5	41.0
48-49	35.53830748494241	40.0	35.0	41.0	26.0	41.0
50-51	35.38755856526842	40.0	35.0	41.0	26.0	41.0
52-53	35.20834877203734	39.0	35.0	41.0	26.0	41.0
54-55	35.03637981482754	39.0	35.0	41.0	26.0	41.0
56-57	34.829132137388314	39.0	34.0	41.0	25.5	41.0
58-59	34.60573856987344	38.5	34.0	41.0	25.0	41.0
60-61	34.36088623383104	38.0	34.0	40.0	24.5	41.0
62-63	34.24529990275413	38.0	34.0	40.0	24.5	41.0
64-65	33.98661194873766	37.5	34.0	40.0	23.5	41.0
66-67	33.71333308995516	37.0	33.0	40.0	23.0	41.0
68-69	33.40926900024175	36.5	33.0	40.0	22.0	41.0
70-71	33.076473685980645	36.0	33.0	39.5	20.5	41.0
72-73	32.739436548866834	35.5	33.0	39.0	20.0	41.0
74-75	32.45240040347119	35.0	33.0	39.0	19.0	41.0
76-77	32.19925715998943	35.0	33.0	38.0	18.0	40.0
78-79	31.86449033879679	35.0	32.0	37.0	16.0	39.5
80-81	31.55026430007264	35.0	32.0	37.0	13.5	39.0
82-83	31.237736686471326	35.0	32.0	36.0	9.5	39.0
84-85	30.955301682097705	35.0	32.0	36.0	7.0	37.5
86-87	30.687940937291508	35.0	31.5	36.0	2.0	37.0
88-89	30.43410219756118	35.0	31.0	35.0	2.0	37.0
90-91	30.213890751753976	35.0	31.0	35.0	2.0	36.0
92-93	29.993794287760963	35.0	31.0	35.0	2.0	36.0
94-95	29.77871922332084	35.0	31.0	35.0	2.0	36.0
96-97	29.583910997176908	35.0	31.0	35.0	2.0	36.0
98-99	29.37278311469062	34.5	30.5	35.0	2.0	35.0
100	29.200545818671955	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27896800424384693
1101	2	0.3045416630801796
1101	3	0.2947273507838304
1101	4	0.401913306778269
1101	5	0.3657950057122292
1101	6	0.39303860308250904
1101	7	0.3413355469992254
1101	8	0.393520668927394
1101	9	0.3065236720341673
1101	10-11	0.4469647306616267
1101	12-13	0.382827175846181
1101	14-15	0.41602345769008764
1101	16-17	0.37028599609135426
1101	18-19	0.3117246126031006
1101	20-21	0.39487915959615094
1101	22-23	0.40910981299210647
1101	24-25	0.31640399445794287
1101	26-27	0.3516262434050077
1101	28-29	0.3927364051547144
1101	30-31	0.48646232310360205
1101	32-33	0.43036967754084543
1101	34-35	0.39730808631382075
1101	36-37	0.3398491178745928
1101	38-39	0.3729982548012387
1101	40-41	0.2591040489598697
1101	42-43	0.33031912019782794
1101	44-45	0.2983174068770822
1101	46-47	0.34203637097983375
1101	48-49	0.33180997772298326
1101	50-51	0.30274135367803723
1101	52-53	0.2688971081293019
1101	54-55	0.29909628091510143
1101	56-57	0.2657200396255561
1101	58-59	0.24260562203362213
1101	60-61	0.2131421184221125
1101	62-63	0.20187775189534563
1101	64-65	0.2584319609763952
1101	66-67	0.27362966022803903
1101	68-69	0.3271759888503425
1101	70-71	0.2722281327486158
1101	72-73	0.2263325502636917
1101	74-75	0.2675861287840249
1101	76-77	0.34211947917876984
1101	78-79	0.30248904385856434
1101	80-81	0.2755578610726701
1101	82-83	0.2808690529117541
1101	84-85	0.26309089314884204
1101	86-87	0.26208991022976846
1101	88-89	0.207025991791582
1101	90-91	0.169951869003917
1101	92-93	0.14288320962747036
1101	94-95	0.17603829851353225
1101	96-97	0.16854384090119012
1101	98-99	0.18984677879934253
1101	100	0.13516120645036622
1102	1	0.15605010403083241
1102	2	0.24609498251119888
1102	3	0.28350990101500173
1102	4	0.31902920080300845
1102	5	0.32589062708711225
1102	6	0.28891428878068126
1102	7	0.1887892968066751
1102	8	0.2039379186859378
1102	9	0.27432146875917596
1102	10-11	0.22674764053949303
1102	12-13	0.1867064743770115
1102	14-15	0.30887257378304156
1102	16-17	0.258377717996666
1102	18-19	0.26112002957440694
1102	20-21	0.21707271371352732
1102	22-23	0.2733486247581709
1102	24-25	0.3312046042446042
1102	26-27	0.27603209346736435
1102	28-29	0.2822537643643912
1102	30-31	0.3297793413837766
1102	32-33	0.2983823764776261
1102	34-35	0.30049809774831715
1102	36-37	0.22523077833966454
1102	38-39	0.18448616254482886
1102	40-41	0.23785436683845518
1102	42-43	0.2600267998029011
1102	44-45	0.20600101597266018
1102	46-47	0.15623183765182347
1102	48-49	0.2286370350081839
1102	50-51	0.19500104878629543
1102	52-53	0.19147004701486736
1102	54-55	0.1369609018564546
1102	56-57	0.15568340205767583
1102	58-59	0.11168002155193335
1102	60-61	0.10173251546587636
1102	62-63	0.13519556303482716
1102	64-65	0.16069361996061104
1102	66-67	0.14499991575356574
1102	68-69	0.13603788777380998
1102	70-71	0.15189024280464025
1102	72-73	0.15216242887279918
1102	74-75	0.15312125678114796
1102	76-77	0.22340907596690585
1102	78-79	0.27201113082951167
1102	80-81	0.22339188940253507
1102	82-83	0.24307552316805214
1102	84-85	0.24467594125228587
1102	86-87	0.19060770503539715
1102	88-89	0.17758606506513175
1102	90-91	0.23638537571497764
1102	92-93	0.1853350857288234
1102	94-95	0.17930252729935958
1102	96-97	0.21588528751033564
1102	98-99	0.20298802461965337
1102	100	0.1757921079592073
1103	1	-0.2643062569708654
1103	2	-0.25799044781048863
1103	3	-0.14416726984964967
1103	4	-0.12621129459869707
1103	5	-0.06555353105288475
1103	6	-0.20574320099368748
1103	7	-0.10698365197507798
1103	8	-0.18337110610251983
1103	9	-0.249466639426835
1103	10-11	-0.2017325368405949
1103	12-13	-0.1507059664441357
1103	14-15	-0.19732121739780695
1103	16-17	-0.20568441202082965
1103	18-19	-0.2975731176496197
1103	20-21	-0.22407840290224357
1103	22-23	-0.3046551714025014
1103	24-25	-0.21720860718184554
1103	26-27	-0.19771319868347348
1103	28-29	-0.28956951459825575
1103	30-31	-0.2282658013484351
1103	32-33	-0.17537686426801002
1103	34-35	-0.2493445569434911
1103	36-37	-0.2845207973852695
1103	38-39	-0.2345289309458778
1103	40-41	-0.1846714074067748
1103	42-43	-0.28571728947508745
1103	44-45	-0.3351517435216991
1103	46-47	-0.38297460674977657
1103	48-49	-0.37798241252349385
1103	50-51	-0.5100035919488946
1103	52-53	-0.5407697111946774
1103	54-55	-0.5221201631304666
1103	56-57	-0.5840854028604525
1103	58-59	-0.6121027413262041
1103	60-61	-0.5918669565272268
1103	62-63	-0.562982679054123
1103	64-65	-0.5709169038434467
1103	66-67	-0.4797730822748605
1103	68-69	-0.4431209234662248
1103	70-71	-0.4783663387197805
1103	72-73	-0.4717780384452084
1103	74-75	-0.45009171770570333
1103	76-77	-0.4089668189406055
1103	78-79	-0.40621897384129824
1103	80-81	-0.39169719251444235
1103	82-83	-0.4475005363368538
1103	84-85	-0.4639899377585941
1103	86-87	-0.5030582956040561
1103	88-89	-0.5045182766939362
1103	90-91	-0.5023004388736361
1103	92-93	-0.5469539985215235
1103	94-95	-0.5929778131860353
1103	96-97	-0.5629227374039552
1103	98-99	-0.6060253748013125
1103	100	-0.5764733085218268
1104	1	-0.2131384595629413
1104	2	-0.17703114901364714
1104	3	-0.15396121467344415
1104	4	-0.026980271747099493
1104	5	-0.10579745163811793
1104	6	-0.05703775158571034
1104	7	-0.01973188185531427
1104	8	-0.05443625575115618
1104	9	0.016385496738273275
1104	10-11	-0.0655472046548411
1104	12-13	-0.14536854923311893
1104	14-15	-0.105356398161085
1104	16-17	-0.11009375471334693
1104	18-19	-0.14976794085691836
1104	20-21	-0.20143778596625594
1104	22-23	-0.12485041516254114
1104	24-25	-0.1447647963945542
1104	26-27	-0.13055916987443794
1104	28-29	-0.09978389468238191
1104	30-31	-0.16494839602567168
1104	32-33	-0.17333191681360205
1104	34-35	-0.1244826658345275
1104	36-37	-0.1594200252755087
1104	38-39	-0.11168216877118908
1104	40-41	-0.12529220041102462
1104	42-43	-0.17963912775259416
1104	44-45	-0.19431652344419348
1104	46-47	-0.3650722172740899
1104	48-49	-0.4096050216577112
1104	50-51	-0.4111569597774647
1104	52-53	-0.4914978273768682
1104	54-55	-0.41810644935223706
1104	56-57	-0.3959766097058264
1104	58-59	-0.4069333494203988
1104	60-61	-0.4449351314928336
1104	62-63	-0.3171147662878866
1104	64-65	-0.25311060714309974
1104	66-67	-0.29607885935372025
1104	68-69	-0.3162585287904065
1104	70-71	-0.2951238575682993
1104	72-73	-0.2970885778726142
1104	74-75	-0.2667087977853626
1104	76-77	-0.2229669176177822
1104	78-79	-0.26866860736522824
1104	80-81	-0.2787683285785256
1104	82-83	-0.35416903819018586
1104	84-85	-0.2984460698795033
1104	86-87	-0.29955648926350165
1104	88-89	-0.34532383488175356
1104	90-91	-0.3392747381677772
1104	92-93	-0.3206919291671255
1104	94-95	-0.3520264320579649
1104	96-97	-0.36927484576716196
1104	98-99	-0.27921652815739506
1104	100	-0.26144547920828387
1105	1	-0.29602730984309744
1105	2	-0.34150921752898356
1105	3	-0.2476769709668396
1105	4	-0.062491034276497714
1105	5	-0.20774767532747518
1105	6	-0.21354677051709103
1105	7	-0.24226013000468072
1105	8	-0.15887064124144246
1105	9	-0.2465174388354754
1105	10-11	-0.34952882862094015
1105	12-13	-0.30428253897008517
1105	14-15	-0.34330664667033517
1105	16-17	-0.37552731183944843
1105	18-19	-0.28236034844933044
1105	20-21	-0.31128081560742515
1105	22-23	-0.3425613532927372
1105	24-25	-0.3099779217784828
1105	26-27	-0.28751711875785446
1105	28-29	-0.25187861673052225
1105	30-31	-0.28392910475792377
1105	32-33	-0.2722775364114014
1105	34-35	-0.3187012578015711
1105	36-37	-0.39933957777270734
1105	38-39	-0.306053713375654
1105	40-41	-0.3331516546214175
1105	42-43	-0.3745800563891777
1105	44-45	-0.5308982646843106
1105	46-47	-0.5636560092585015
1105	48-49	-0.5578445926457221
1105	50-51	-0.5083803885226317
1105	52-53	-0.4628130804467858
1105	54-55	-0.5056795129346128
1105	56-57	-0.573085913445567
1105	58-59	-0.4862015967010649
1105	60-61	-0.5299137398410068
1105	62-63	-0.42808818727683473
1105	64-65	-0.3809354220797161
1105	66-67	-0.4395130648997281
1105	68-69	-0.37510736628831864
1105	70-71	-0.3111516186271146
1105	72-73	-0.31635440521297653
1105	74-75	-0.30749177257455074
1105	76-77	-0.4845115065692056
1105	78-79	-0.4961124368276906
1105	80-81	-0.4896838106120214
1105	82-83	-0.5140442561399361
1105	84-85	-0.482912069718175
1105	86-87	-0.44852475702058925
1105	88-89	-0.4951267493585796
1105	90-91	-0.49234402912526676
1105	92-93	-0.5039124353690028
1105	94-95	-0.5218664522861864
1105	96-97	-0.5673446331226621
1105	98-99	-0.6409775931793291
1105	100	-0.6165047056960802
1106	1	0.012181651656469228
1106	2	-0.0990710890936981
1106	3	0.005891964811912231
1106	4	0.07582569695750152
1106	5	0.04159481306226809
1106	6	0.014252432930533132
1106	7	0.14491716221579765
1106	8	0.06346649305010033
1106	9	-0.013942134722270794
1106	10-11	-0.04348467360407682
1106	12-13	-0.024757568395550322
1106	14-15	-0.03267967627540003
1106	16-17	0.060521687604619956
1106	18-19	0.021280983434778022
1106	20-21	-8.57642276343995E-4
1106	22-23	0.07292090523148431
1106	24-25	0.10207768536820083
1106	26-27	0.030773349553797402
1106	28-29	-0.008665000766562514
1106	30-31	0.09730693147346159
1106	32-33	0.023826063451529933
1106	34-35	0.09498018326685553
1106	36-37	-0.012497085628751847
1106	38-39	0.028915771688467373
1106	40-41	-0.010014938308131605
1106	42-43	0.034925375045659734
1106	44-45	0.03748027729626813
1106	46-47	0.02008784022607557
1106	48-49	0.015486816782257051
1106	50-51	-0.033623368232802875
1106	52-53	0.032935337756129
1106	54-55	-0.008659132252134327
1106	56-57	-0.02198199297346548
1106	58-59	-0.06978868932093008
1106	60-61	-0.06772446794970222
1106	62-63	0.011180982223756075
1106	64-65	0.015122669300055236
1106	66-67	0.09443036192134002
1106	68-69	-0.02761167311324897
1106	70-71	-0.07802268177916005
1106	72-73	-0.055181363053847576
1106	74-75	-0.07160334426831128
1106	76-77	-0.09218398830049068
1106	78-79	-0.0835692146558884
1106	80-81	-0.03198785383164093
1106	82-83	-0.01222616933728915
1106	84-85	-0.05647047720207965
1106	86-87	-0.027849023996886046
1106	88-89	-0.08933236916444898
1106	90-91	-0.012796116598867258
1106	92-93	-0.001656758334018349
1106	94-95	-6.021611508266744E-4
1106	96-97	0.03369835283450584
1106	98-99	-0.022774244968331914
1106	100	-0.08789555486440292
1107	1	-0.46899434281013086
1107	2	-0.5501787622699545
1107	3	-0.4595411781881893
1107	4	-0.43763624620742547
1107	5	-0.30107185115164725
1107	6	-0.39923750835068716
1107	7	-0.4670638976655894
1107	8	-0.4819985847265329
1107	9	-0.5558659474696981
1107	10-11	-0.4377686245393093
1107	12-13	-0.4248535750768312
1107	14-15	-0.5104377298728409
1107	16-17	-0.5245324138802729
1107	18-19	-0.5766735352625147
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2216	56-57	0.36568251178346856
2216	58-59	0.39662322149418117
2216	60-61	0.37682243973434026
2216	62-63	0.27129610692224304
2216	64-65	0.31112451079765435
2216	66-67	0.24951406926651032
2216	68-69	0.32923512226408036
2216	70-71	0.2346100204840127
2216	72-73	0.22873094082579826
2216	74-75	0.17610930125003676
2216	76-77	0.23410656260762863
2216	78-79	0.19873038836836798
2216	80-81	0.14055434760363283
2216	82-83	0.1435522111626888
2216	84-85	0.1979815130978828
2216	86-87	0.19778040702449573
2216	88-89	0.22546051348798457
2216	90-91	0.27197964345098313
2216	92-93	0.25760730465965054
2216	94-95	0.22281316084841407
2216	96-97	0.1918095840262417
2216	98-99	0.2820873069096379
2216	100	0.24688667004290465
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95023.0
3	21527.0
4	16694.0
5	14732.0
6	9001.0
7	6718.0
8	7183.0
9	7446.0
10	7661.0
11	8034.0
12	9477.0
13	10801.0
14	11843.0
15	12296.0
16	12538.0
17	12223.0
18	11609.0
19	10749.0
20	10124.0
21	10956.0
22	13233.0
23	20422.0
24	32883.0
25	40610.0
26	43195.0
27	32203.0
28	31412.0
29	38721.0
30	44918.0
31	52401.0
32	66920.0
33	90084.0
34	128605.0
35	207984.0
36	350863.0
37	616965.0
38	901042.0
39	458140.0
40	1575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06217441401362	22.058563071633884	14.03090030419944	21.848362210153056
2	14.531133824446663	23.614503489262102	39.484325245187115	22.370037441104124
3	17.260215976625332	30.17330835350584	30.045865152132624	22.520610517736205
4	11.715099452557372	20.363742653008917	38.20950839594414	29.711649498489567
5	11.914156049171362	41.87555317205743	33.610078971296545	12.600211807474668
6	26.963664525156698	40.93764995460367	18.778717924818157	13.319967595421472
7	24.354966267076833	36.783285063986256	22.483063054717505	16.37868561421941
8	23.634986032937014	38.73730616762835	20.30544302672339	17.322264772711254
9	24.883786953246414	20.101311946853652	20.71562097216553	34.2992801277344
10-11	22.237020029973113	30.600277810101534	28.23073629170262	18.931965868222733
12-13	22.9011823516458	28.853732654558595	27.913975902470696	20.331109091324908
14-15	20.956829642048287	30.149707256466808	25.49390133221105	23.399561769273852
16-17	20.176422050584524	33.07583363669013	26.21398210408271	20.533762208642642
18-19	20.297988626394893	31.978278620674576	27.88076600167453	19.842966751256004
20-21	22.14360057788203	30.62092975488343	27.24038603716419	19.995083630070347
22-23	21.833344792103862	30.753839431269096	27.175783197376703	20.237032579250343
24-25	21.201980022855267	30.888672977635924	27.288716415591924	20.62063058391688
26-27	20.996633442195677	30.900556010646984	27.371805060408892	20.73100548674845
28-29	20.801181868825044	30.891727853156453	27.603946792889168	20.703143485129335
30-31	20.994559695834337	31.034316594603485	27.31968824515417	20.651435464408017
32-33	20.846855451303835	30.891589556672074	27.519889416140813	20.74166557588328
34-35	21.09921539273227	30.700456311523087	27.483132123215043	20.717196172529597
36-37	21.00070762103103	30.85757080418059	27.351991185737788	20.78973038905059
38-39	20.979594455335253	30.822433095372215	27.33702291020674	20.86094953908579
40-41	20.99279546304185	30.65264586609	27.48743661490472	20.86712205596343
42-43	20.94020972920131	30.712017994461092	27.586405303745714	20.761366972591876
44-45	21.004507154910613	30.641906326560854	27.48422208059938	20.869364437929157
46-47	21.060258475007814	30.60672177716096	27.40837981015706	20.92463993767416
48-49	20.912924993138198	30.603073513088642	27.424374856506283	21.059626637266877
50-51	20.942473146894795	30.642964105234537	27.435048355009055	20.979514392861613
52-53	21.023699883943124	30.587286910200056	27.393521989526686	20.995491216330127
54-55	21.0214510150684	30.61598322942348	27.379249302940188	20.983316452567927
56-57	20.934132994060082	30.643149602674868	27.294053680067144	21.128663723197903
58-59	21.107679772691572	30.33795614599894	27.42338036127448	21.130983720035008
60-61	21.093744493366724	30.669852584415384	27.284031011632454	20.952371910585438
62-63	20.892941953853857	30.687900093693806	27.392262911089844	21.026895041362494
64-65	21.13710159745071	30.44287263229693	27.443535156199435	20.976490614052928
66-67	20.91934596478106	30.555311429074866	27.328847958747023	21.196494647397056
68-69	21.09965192858948	30.61509492745504	27.277843950837056	21.00740919311843
70-71	21.08621235221356	30.566967753070795	27.34533843454247	21.00148146017317
72-73	21.059784328367652	30.698214472689532	27.396519268805967	20.84548193013684
74-75	21.092395033103532	30.581868497777037	27.33624071008266	20.98949575903677
76-77	21.108160443077757	30.460358872961802	27.147229512587423	21.28425117137302
78-79	21.12134864009889	30.490094870666674	27.30857541244942	21.079981076785014
80-81	20.946295651113193	30.559400282113902	27.390883687423777	21.103420379349124
82-83	20.9854537759895	30.59730273209392	27.485575113198912	20.931668378717667
84-85	21.05890356235711	30.307015026435113	27.38181087409439	21.252270537113382
86-87	21.163786748179447	30.52398094039378	27.22241121999461	21.089821091432167
88-89	21.070421165606092	30.42079735248674	27.258405136792547	21.250376345114617
90-91	21.067783227507377	30.346543633231164	27.216832189196992	21.368840950064467
92-93	21.028609088330548	30.434380499839193	27.28507081505927	21.251939596770992
94-95	20.98597537628986	30.356681049052092	27.372856270404572	21.28448730425347
96-97	21.084650403618042	30.48351460894077	27.126026377028428	21.30580861041276
98-99	20.883282780929772	30.58734480057285	27.199331632302254	21.330040786195124
100	20.96819857119429	30.550979246927895	27.15850076101311	21.322321420864707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32692.0
1	24322.0
2	13881.5
3	9876.5
4	6623.5
5	4563.0
6	3534.0
7	3395.5
8	3586.0
9	3396.5
10	2736.0
11	2176.0
12	2063.0
13	2081.5
14	2112.5
15	2275.5
16	2557.5
17	2944.5
18	3590.5
19	4593.0
20	6088.0
21	8314.5
22	11412.0
23	15588.0
24	21078.5
25	27348.5
26	35292.5
27	44227.5
28	54112.5
29	65636.5
30	75187.0
31	82344.5
32	87468.5
33	93846.0
34	100333.5
35	103961.0
36	106602.0
37	109337.5
38	111414.0
39	111292.0
40	110017.0
41	108418.5
42	106870.0
43	105068.5
44	102889.5
45	101072.0
46	101601.5
47	100152.0
48	97363.5
49	97768.0
50	97941.5
51	94871.5
52	91089.5
53	87224.0
54	84552.5
55	82737.5
56	74877.5
57	64906.0
58	59008.5
59	55346.5
60	50138.5
61	42360.5
62	35825.0
63	29762.0
64	23252.5
65	18938.5
66	15571.0
67	12083.0
68	9208.5
69	6973.5
70	5316.5
71	4141.5
72	3214.5
73	2523.5
74	2044.0
75	1554.0
76	1058.0
77	807.0
78	635.0
79	469.5
80	340.0
81	239.5
82	170.0
83	123.5
84	114.5
85	101.0
86	83.5
87	72.0
88	53.5
89	51.5
90	47.0
91	31.0
92	27.0
93	23.5
94	22.5
95	22.5
96	17.0
97	14.0
98	15.0
99	18.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18448832086595104
2	0.17698575749013096
3	0.19190464788112951
4	0.18112510280092825
5	0.1951816295855107
6	0.07930870633673402
7	0.08134963353858546
8	0.06950650667713767
9	0.06387239778188582
10-11	0.07748337003648662
12-13	0.07604609735912644
14-15	0.07358836108084055
16-17	0.06989457030002492
18-19	0.0748675337636911
20-21	0.07672161551748571
22-23	0.07416327015178462
24-25	0.07440760650693586
26-27	0.07785706093260024
28-29	0.07535620647399356
30-31	0.08078909719441499
32-33	0.07807265183420427
34-35	0.07695157914586334
36-37	0.08855036965215989
38-39	0.08316059711205925
40-41	0.07869067908546915
42-43	0.08199640624339753
44-45	0.09273283314327799
46-47	0.08293063348368164
48-49	0.08764488786542299
50-51	0.08497156068553308
52-53	0.08419543343975858
54-55	0.0851296606800427
56-57	0.0791506063422244
58-59	0.08638008790934604
60-61	0.08305998802464405
62-63	0.07456570650144546
64-65	0.07790017911292105
66-67	0.07756960639712822
68-69	0.07636229734814567
70-71	0.07217983385702759
72-73	0.06942027031649607
74-75	0.07462319740853987
76-77	0.07939494269737563
78-79	0.07639104280169287
80-81	0.08364926982236173
82-83	0.08510091522649549
84-85	0.08728556969608293
86-87	0.0826000607678888
88-89	0.08466973342328744
90-91	0.08334744256011609
92-93	0.0739620519769542
94-95	0.07381832470921817
96-97	0.07377520652889737
98-99	0.08278690621594563
100	0.09143928773365383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3478811.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.84608041530024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81470021831385	37.42279342322393
2	15.286111773274325	16.156221840154455
3	5.87742489511607	9.317966059265745
4	2.828531114668995	5.979071309719062
5	1.5434664433713372	4.078307589235957
6	0.9254480826850155	2.9343782278654653
7	0.6105571090506692	2.258588505811743
8	0.4258577102388429	1.8003928640606015
9	0.2933227378303471	1.3950861291916699
>10	1.3086730282018189	11.531302482468172
>50	0.05660486672686429	2.027646607110138
>100	0.02636748098357918	2.594438493092653
>500	0.0020650463463699345	0.7696508990018098
>1k	8.151498674199395E-4	0.7158902052486028
>5k	0.0	0.0
>10k+	5.434332449466261E-5	1.0182653645498918
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35457	1.0192275464231888	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3856	0.11084246887801608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	2.874545354720334E-5
12-13	0.0	0.0	0.0	0.0	2.874545354720334E-5
14-15	0.0	0.0	0.0	2.874545354720334E-5	2.874545354720334E-5
16-17	0.0	0.0	0.0	5.749090709440668E-5	2.874545354720334E-5
18-19	0.0	0.0	0.0	1.2935454096241504E-4	2.874545354720334E-5
20-21	0.0	0.0	0.0	2.0121817483042338E-4	2.874545354720334E-5
22-23	0.0	0.0	0.0	5.461636173968635E-4	2.874545354720334E-5
24-25	0.0	0.0	0.0	0.001178563595435337	2.874545354720334E-5
26-27	0.0	0.0	0.0	0.001796590846700209	2.874545354720334E-5
28-29	0.0	0.0	0.0	0.0037512816879100358	2.874545354720334E-5
30-31	0.0	0.0	0.0	0.008853599692538629	2.874545354720334E-5
32-33	0.0	0.0	0.0	0.01618369034707548	2.874545354720334E-5
34-35	0.0	0.0	0.0	0.028041189935296862	2.874545354720334E-5
36-37	0.0	0.0	0.0	0.04708505291031907	2.874545354720334E-5
38-39	0.0	0.0	0.0	0.07279786110829245	2.874545354720334E-5
40-41	0.0	0.0	0.0	0.10121274193970296	2.874545354720334E-5
42-43	0.0	0.0	0.0	0.12876525916469736	2.874545354720334E-5
44-45	0.0	0.0	0.0	0.1624980489023405	2.874545354720334E-5
46-47	0.0	0.0	0.0	0.20564497467669268	2.874545354720334E-5
48-49	0.0	0.0	0.0	0.2546416002479008	2.874545354720334E-5
50-51	0.0	0.0	0.0	0.30274711675914556	2.874545354720334E-5
52-53	0.0	0.0	0.0	0.34898417878982213	2.874545354720334E-5
54-55	0.0	0.0	0.0	0.4019045587702235	2.874545354720334E-5
56-57	0.0	0.0	0.0	0.4697438291416234	2.874545354720334E-5
58-59	0.0	0.0	0.0	0.5456030810526931	4.311818032080501E-5
60-61	0.0	0.0	0.0	0.6302728144759804	5.749090709440668E-5
62-63	0.0	0.0	0.0	0.7161929751285712	5.749090709440668E-5
64-65	0.0	0.0	0.0	0.8071435901519226	5.749090709440668E-5
66-67	0.0	0.0	0.0	0.9132430591946501	5.749090709440668E-5
68-69	0.0	0.0	0.0	1.0375671457863045	5.749090709440668E-5
70-71	0.0	0.0	0.0	1.1776581136486	5.749090709440668E-5
72-73	0.0	0.0	0.0	1.3237999994825818	5.749090709440668E-5
74-75	0.0	0.0	0.0	1.4720259306987358	5.749090709440668E-5
76-77	0.0	0.0	0.0	1.6365361613493805	5.749090709440668E-5
78-79	0.0	0.0	0.0	1.831760909115212	5.749090709440668E-5
80-81	0.0	0.0	0.0	2.0443766562771017	5.749090709440668E-5
82-83	1.437272677360167E-5	0.0	0.0	2.275403866435975	5.749090709440668E-5
84-85	2.874545354720334E-5	0.0	0.0	2.507882721998982	7.186363386800835E-5
86-87	2.874545354720334E-5	0.0	0.0	2.7527221225872864	8.623636064161002E-5
88	2.874545354720334E-5	0.0	0.0	2.9540840246854456	8.623636064161002E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5640	0.0	46.891438	1
GTATCAA	9745	0.0	38.53518	1
TCAACGC	11270	0.0	31.686853	4
ATCAACG	11270	0.0	31.604723	3
TATCAAC	12185	0.0	30.389896	2
CAACGCA	12535	0.0	28.676773	5
AACGCAG	12895	0.0	27.733566	6
GTACATG	20915	0.0	26.842247	1
TACATGG	20960	0.0	25.411785	2
ACGCAGA	14365	0.0	24.66689	7
CGCAGAG	14415	0.0	24.222715	8
CATGGGT	9670	0.0	23.74404	4
ACATGGG	21515	0.0	23.61905	3
ATGGGTG	5345	0.0	21.566498	5
GCAGAGT	15910	0.0	21.326921	9
GTGGTAT	2805	0.0	21.306257	1
TACCTGG	2540	0.0	20.74743	2
CAGAGTA	15355	0.0	18.669865	10-11
CATGGGG	9860	0.0	18.37152	4
ATGGGTA	4060	0.0	17.730732	5
>>END_MODULE