FastQCFastQC Report
Wed 25 May 2016
SRR2431421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478811
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426461.2258786119740337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337730.9708202026496984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222590.6398450505071991No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74870.21521721070791142No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.18796652074516265No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58280.16752850327310106No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.1454232494953017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39800.1144069051178693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121100.063.5241781
GTATCAA242050.046.1573941
GTGGTAT37600.037.1422771
TATCAAC312800.035.5499762
ATCAACG313500.035.3335843
TCAACGC316500.035.0427324
CAACGCA328100.033.8181235
TGGTATC41350.033.771932
AACGCAG337000.032.9963726
ACGCAGA373350.029.6201427
CGCAGAG374550.029.3872188
GCAGAGT399550.027.2661429
CATGGGT90500.026.4950854
GTACATG196400.025.8572431
TACATGG199200.024.9966372
ACATGGG199600.024.3562223
GAGTACT278900.024.02992212-13
CAGAGTA390150.023.3274510-11
AGAGTAC380700.022.28310810-11
AGTACTT284300.022.2179412-13
Produced by FastQC (version 0.11.5)