##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431421_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3478811
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.661472554847045	34.0	31.0	34.0	31.0	34.0
2	32.78293560644715	34.0	31.0	34.0	31.0	34.0
3	32.85923926307005	34.0	31.0	34.0	31.0	34.0
4	36.32907996439013	37.0	37.0	37.0	35.0	37.0
5	36.240003552938056	37.0	37.0	37.0	35.0	37.0
6	36.28401399213697	37.0	37.0	37.0	35.0	37.0
7	36.23879796861629	37.0	37.0	37.0	35.0	37.0
8	36.25905948900357	37.0	37.0	37.0	35.0	37.0
9	38.05237565363568	39.0	39.0	39.0	35.0	39.0
10-11	38.0285610514627	39.0	39.0	39.0	35.0	39.0
12-13	38.00656847986281	39.0	39.0	39.0	35.0	39.0
14-15	39.45497039074557	41.0	39.0	41.0	36.0	41.0
16-17	39.48603229666688	41.0	39.0	41.0	36.5	41.0
18-19	39.5697071499429	41.0	40.0	41.0	37.0	41.0
20-21	39.57112315098463	41.0	40.0	41.0	37.0	41.0
22-23	39.509129412319325	41.0	40.0	41.0	36.0	41.0
24-25	39.45109550360741	41.0	39.0	41.0	36.0	41.0
26-27	39.32723393136333	41.0	39.0	41.0	35.5	41.0
28-29	39.24113051844438	41.0	39.0	41.0	35.0	41.0
30-31	39.02863305882383	41.0	38.5	41.0	35.0	41.0
32-33	38.770852455048576	41.0	38.0	41.0	35.0	41.0
34-35	38.62893011434079	41.0	38.0	41.0	35.0	41.0
36-37	38.48317384876614	41.0	38.0	41.0	35.0	41.0
38-39	38.336398556863244	40.0	38.0	41.0	35.0	41.0
40-41	38.18630891416636	40.0	38.0	41.0	34.0	41.0
42-43	37.99496695853842	40.0	38.0	41.0	34.0	41.0
44-45	37.719452422106286	40.0	37.0	41.0	33.0	41.0
46-47	37.310361499949266	40.0	37.0	41.0	32.5	41.0
48-49	36.93228117307896	40.0	37.0	41.0	31.0	41.0
50-51	36.75356867619425	40.0	36.5	41.0	31.0	41.0
52-53	36.681058413348694	40.0	36.0	41.0	31.0	41.0
54-55	36.5345379498915	40.0	36.0	41.0	31.0	41.0
56-57	36.36692464753044	40.0	35.0	41.0	31.0	41.0
58-59	36.17464113457155	39.5	35.0	41.0	30.5	41.0
60-61	36.009768998660746	39.0	35.0	41.0	30.0	41.0
62-63	35.84333354125878	39.0	35.0	41.0	30.0	41.0
64-65	35.606840958016974	39.0	35.0	41.0	30.0	41.0
66-67	35.346021528619985	38.0	35.0	41.0	29.0	41.0
68-69	35.05836534379131	37.0	35.0	40.5	29.0	41.0
70-71	34.76278116287433	37.0	35.0	40.0	29.0	41.0
72-73	34.45465232230207	36.0	35.0	39.0	29.0	41.0
74-75	34.12520024801577	36.0	35.0	39.0	28.0	41.0
76-77	33.804743488507995	35.5	35.0	39.0	28.0	40.5
78-79	33.49611807022572	35.0	34.0	37.0	27.5	39.5
80-81	33.24314758691979	35.0	34.0	37.0	28.0	39.0
82-83	33.05520578726467	35.0	34.0	37.0	28.5	39.0
84-85	32.803482425460885	35.0	34.0	36.0	28.0	38.0
86-87	32.572413390667094	35.0	34.0	36.0	27.5	37.0
88-89	32.348813718250284	35.0	34.0	36.0	27.0	37.0
90-91	32.17133943177712	35.0	34.0	35.0	27.0	36.5
92-93	32.01447204231561	35.0	34.0	35.0	26.0	36.0
94-95	31.87819904559345	35.0	34.0	35.0	26.0	36.0
96-97	31.741431483343014	35.0	34.0	35.0	25.5	36.0
98-99	31.63144864725333	35.0	34.0	35.0	25.0	36.0
100	31.532230408607997	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0015451385413030039
1101	2	0.02062317069503905
1101	3	-0.007087723227968468
1101	4	0.04857961292479018
1101	5	0.04523245258472741
1101	6	0.06162675893587277
1101	7	0.03604186891956829
1101	8	0.08317622613939335
1101	9	0.06200192620620726
1101	10-11	0.05970078082355457
1101	12-13	0.04706280411255648
1101	14-15	0.07733677180028309
1101	16-17	0.025649934608814817
1101	18-19	0.01192879672793623
1101	20-21	0.02498882493154042
1101	22-23	0.027651217135947093
1101	24-25	0.014758697726946934
1101	26-27	0.03225941031249846
1101	28-29	0.032678677314706306
1101	30-31	0.05871440366850322
1101	32-33	0.03541882015163367
1101	34-35	0.06600194095396716
1101	36-37	0.06551943575286145
1101	38-39	0.0385771706513367
1101	40-41	0.03978026754169406
1101	42-43	0.036740626354642814
1101	44-45	0.07606968366782496
1101	46-47	0.043379474227805304
1101	48-49	0.09898940199532547
1101	50-51	0.0018019179237782623
1101	52-53	0.044221850251851436
1101	54-55	0.0355135130631794
1101	56-57	0.06536858472144758
1101	58-59	0.09049953297027002
1101	60-61	-0.0069831661814134804
1101	62-63	0.06109214370751204
1101	64-65	0.10289441955737999
1101	66-67	0.06886214972308125
1101	68-69	0.07454183870924425
1101	70-71	0.0467013737702473
1101	72-73	0.02843060270795661
1101	74-75	0.0318582764607811
1101	76-77	-0.008599772051510968
1101	78-79	0.0034810786543459926
1101	80-81	0.02439924884971134
1101	82-83	0.014413630231132402
1101	84-85	-0.0026349116659503125
1101	86-87	0.022354810336487674
1101	88-89	0.057887722756937876
1101	90-91	0.038567604964995894
1101	92-93	0.02690634116515156
1101	94-95	0.009598885611946884
1101	96-97	0.03640771153475342
1101	98-99	0.026872936009471715
1101	100	0.05933504061396988
1102	1	-0.0030826341529675005
1102	2	0.02629521310141314
1102	3	0.01550364288372208
1102	4	0.04547212876986606
1102	5	0.0020058107323919216
1102	6	-0.0020278871203700533
1102	7	-0.03533691897968083
1102	8	0.032876135246574734
1102	9	0.050095094007254204
1102	10-11	0.08141263318838554
1102	12-13	0.01666447493246892
1102	14-15	0.008966618838023521
1102	16-17	0.009246505706805408
1102	18-19	-0.010725449959188893
1102	20-21	0.07240858427252306
1102	22-23	0.06993705356239843
1102	24-25	0.0690178418059304
1102	26-27	0.12756628301242046
1102	28-29	0.12032252109981556
1102	30-31	0.10272161074374253
1102	32-33	0.11469789892220916
1102	34-35	0.07236618608159517
1102	36-37	0.15005674872088548
1102	38-39	0.08497884461726812
1102	40-41	0.015889919030769306
1102	42-43	0.07621864448918814
1102	44-45	0.016588413062322616
1102	46-47	0.04509019927063207
1102	48-49	0.14896549704882034
1102	50-51	0.10518411829584551
1102	52-53	0.0967111226191264
1102	54-55	0.13429623568961802
1102	56-57	0.15949588058738584
1102	58-59	0.1332027129001574
1102	60-61	0.08267355116019104
1102	62-63	0.12534048090756045
1102	64-65	0.10926859528756694
1102	66-67	0.15195738030889316
1102	68-69	0.12883089376315837
1102	70-71	0.14870459080881915
1102	72-73	0.17596815488433748
1102	74-75	0.18955342491653937
1102	76-77	0.18635183929998078
1102	78-79	0.19943705128814315
1102	80-81	0.1701282992704023
1102	82-83	0.17901668458259223
1102	84-85	0.16983381547628795
1102	86-87	0.20503877097538492
1102	88-89	0.2074225177213762
1102	90-91	0.20818587180791326
1102	92-93	0.2019868366434352
1102	94-95	0.21322855262772222
1102	96-97	0.23180931821091022
1102	98-99	0.23433202212551052
1102	100	0.2917536436355874
1103	1	-0.0911623951942957
1103	2	-0.0756740943550227
1103	3	-0.03289813982209466
1103	4	-0.00766888247587616
1103	5	-0.038668053620838805
1103	6	-0.04155379997852293
1103	7	-0.07459580454211334
1103	8	-0.03822649938702938
1103	9	-0.004090057854448048
1103	10-11	-0.02112936031621615
1103	12-13	-0.011145570682813855
1103	14-15	-0.03959726915876871
1103	16-17	-0.0028939504126412885
1103	18-19	-0.037783243689908375
1103	20-21	-0.13946261436336727
1103	22-23	-0.08790607859594957
1103	24-25	-0.07655066581466485
1103	26-27	-0.14058053128527348
1103	28-29	-0.1218464540427604
1103	30-31	-0.12881926929394183
1103	32-33	-0.13984675366472743
1103	34-35	-0.14858050802405387
1103	36-37	-0.17536916656190726
1103	38-39	-0.21967654155821492
1103	40-41	-0.22021486979762273
1103	42-43	-0.1661949171799293
1103	44-45	-0.18280323772550133
1103	46-47	-0.09022265858374112
1103	48-49	-0.01774453658613595
1103	50-51	-0.1272439757058592
1103	52-53	-0.09866115029666389
1103	54-55	-0.09909710900920743
1103	56-57	-0.0725730817415311
1103	58-59	-0.06173915757108972
1103	60-61	-0.06713116256007368
1103	62-63	-0.05810155688730134
1103	64-65	-0.06712831084663407
1103	66-67	-0.04350798906041575
1103	68-69	-0.06608050011973177
1103	70-71	-0.07250061857665457
1103	72-73	-0.10203597064571568
1103	74-75	-0.08569735117494304
1103	76-77	-0.13134986799612136
1103	78-79	-0.06528088887861827
1103	80-81	-0.08435651126542609
1103	82-83	-0.07086360155289384
1103	84-85	-0.04593677930647999
1103	86-87	-0.038379208568862566
1103	88-89	-0.06904011942972232
1103	90-91	-0.08598413937993143
1103	92-93	-0.052888018667182735
1103	94-95	-0.11353595155965834
1103	96-97	-0.07800538135401425
1103	98-99	-0.09316894008943066
1103	100	0.018319240464215625
1104	1	-0.10329886573093461
1104	2	-0.04982036055942274
1104	3	-0.04925266812561091
1104	4	-0.029837415263720857
1104	5	-0.02832586309713747
1104	6	-0.06353296656360641
1104	7	-0.08352295650731634
1104	8	-0.11508274631805193
1104	9	-0.09432978857539354
1104	10-11	-0.09516463427460309
1104	12-13	-0.0890043111704486
1104	14-15	-0.12742567424580642
1104	16-17	-0.04761479972554383
1104	18-19	-0.06513207377799546
1104	20-21	-0.056187279976953164
1104	22-23	-0.05679218555417975
1104	24-25	-0.0629919125616425
1104	26-27	-0.11864246061205108
1104	28-29	-0.13471223506869734
1104	30-31	-0.13678694233729516
1104	32-33	-0.08723637031955178
1104	34-35	-0.05190150409967487
1104	36-37	0.039836986508333894
1104	38-39	-0.05872783881068244
1104	40-41	-0.11027974221038761
1104	42-43	-0.08762231388018904
1104	44-45	-0.09102792174316221
1104	46-47	-0.1265699074875286
1104	48-49	-0.12888851833555748
1104	50-51	-0.09160927783423034
1104	52-53	-0.050554792745387545
1104	54-55	0.054709695956915994
1104	56-57	-0.033720293993788175
1104	58-59	-0.009669482085868708
1104	60-61	-0.0451730823480645
1104	62-63	-0.019333584523174352
1104	64-65	-0.023089175947895058
1104	66-67	-0.04846502924630158
1104	68-69	-0.09227543375148883
1104	70-71	-0.12539444428300328
1104	72-73	-0.04249296736665542
1104	74-75	-0.002902930977768392
1104	76-77	-0.05770472130779325
1104	78-79	-0.01759598468554202
1104	80-81	-0.015621533103619356
1104	82-83	-0.027171168134536572
1104	84-85	0.010100208188923432
1104	86-87	-0.07475754861511774
1104	88-89	-0.10655444132563474
1104	90-91	-0.07981293917568877
1104	92-93	-0.08718450521145371
1104	94-95	-0.15197453010707918
1104	96-97	-0.1475239816104157
1104	98-99	-0.14537308280795713
1104	100	-0.07501758311743956
1105	1	-0.14511295226551368
1105	2	-0.1427074424216599
1105	3	-0.1658522176444137
1105	4	-0.04489968218933882
1105	5	-0.09990397442680177
1105	6	-0.049622187901277925
1105	7	-0.04931803099804455
1105	8	-0.04016977109451858
1105	9	-0.012000316804929412
1105	10-11	-0.05801056163915774
1105	12-13	-0.08529567508603009
1105	14-15	-0.11787396818790086
1105	16-17	-0.1526582810889039
1105	18-19	-0.1462902013646925
1105	20-21	-0.07023355978409995
1105	22-23	-0.15471747455089968
1105	24-25	-0.1927087795455975
1105	26-27	-0.15007779546353817
1105	28-29	-0.09604856954168639
1105	30-31	-0.10007885229069302
1105	32-33	-0.15471182964283514
1105	34-35	-0.10232111554412882
1105	36-37	-0.11577169052891634
1105	38-39	-0.02708021624690815
1105	40-41	-0.11915045224569099
1105	42-43	-0.16868116186451232
1105	44-45	-0.1617720844127959
1105	46-47	-0.07050035592107662
1105	48-49	-0.10542324137649928
1105	50-51	-0.08689155766619905
1105	52-53	-0.10603510435924335
1105	54-55	-0.09343113836404626
1105	56-57	-0.12030040642793693
1105	58-59	-0.08916405583509146
1105	60-61	-0.11269802634816273
1105	62-63	-0.21419485679128059
1105	64-65	-0.17662500347439902
1105	66-67	-0.18732438417708863
1105	68-69	-0.11026706944499409
1105	70-71	-0.11144511721255412
1105	72-73	-0.004559937663444202
1105	74-75	-0.134581113044149
1105	76-77	-0.14682840817993537
1105	78-79	-0.11228615476390758
1105	80-81	-0.10904177745554477
1105	82-83	-0.13438096144681566
1105	84-85	-0.1366970938878822
1105	86-87	-0.11180249125012409
1105	88-89	-0.05587445361148724
1105	90-91	-0.04846470666286251
1105	92-93	-0.11661780483108686
1105	94-95	-0.09198754504245699
1105	96-97	-0.1519916412799418
1105	98-99	-0.10876179037368416
1105	100	-0.09342650338971126
1106	1	-0.18138766582711696
1106	2	-0.09176538453420591
1106	3	-0.12394469364527794
1106	4	-0.051437181149424305
1106	5	-0.04535423735636357
1106	6	-0.10095873773806119
1106	7	-0.0989052335410534
1106	8	-0.07669053530666048
1106	9	-0.1252854301173727
1106	10-11	-0.08017672564269418
1106	12-13	-0.08126581668567212
1106	14-15	-0.13830238539369333
1106	16-17	-0.14600809964062478
1106	18-19	-0.16459248040790442
1106	20-21	-0.09515606171301272
1106	22-23	-0.12741934871051797
1106	24-25	-0.06444911524367569
1106	26-27	-0.11714901785398268
1106	28-29	-0.10207711081647375
1106	30-31	-0.14290230865886144
1106	32-33	-0.18208988252548153
1106	34-35	-0.20669978847141124
1106	36-37	-0.21557807862550504
1106	38-39	-0.18679875517607059
1106	40-41	-0.2330783419104847
1106	42-43	-0.22057340924925484
1106	44-45	-0.22136105443591703
1106	46-47	-0.2523405715146083
1106	48-49	-0.28120553903455914
1106	50-51	-0.4145748049581428
1106	52-53	-0.4393879908479761
1106	54-55	-0.35401259276741825
1106	56-57	-0.3208163882323518
1106	58-59	-0.33391233483205696
1106	60-61	-0.40481685582281557
1106	62-63	-0.41692822273114416
1106	64-65	-0.4126737175988282
1106	66-67	-0.36568679733855447
1106	68-69	-0.38024578910462736
1106	70-71	-0.40817921717474803
1106	72-73	-0.3407481693393848
1106	74-75	-0.3797010021058611
1106	76-77	-0.37625429024560475
1106	78-79	-0.3350574817315106
1106	80-81	-0.34272183172033266
1106	82-83	-0.3758838608950654
1106	84-85	-0.33841977130338563
1106	86-87	-0.3893363999880819
1106	88-89	-0.4535678582827387
1106	90-91	-0.4562990847666484
1106	92-93	-0.35365975042273234
1106	94-95	-0.38815821379265714
1106	96-97	-0.4020630275039281
1106	98-99	-0.41190645959661154
1106	100	-0.37901382186259625
1107	1	-0.22062708099392836
1107	2	-0.21972078622071933
1107	3	-0.1933914641122314
1107	4	-0.21326702912811868
1107	5	-0.2591308190107924
1107	6	-0.22267556309751058
1107	7	-0.19316418484419984
1107	8	-0.1791964586926298
1107	9	-0.17413140000743965
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2216	24-25	-0.08959619972154087
2216	26-27	-0.07073337464521501
2216	28-29	-0.02976540756456103
2216	30-31	-0.07520865762134576
2216	32-33	0.02861619353748779
2216	34-35	0.021917142290561742
2216	36-37	-0.052534209035734136
2216	38-39	-0.039656476341932034
2216	40-41	-0.05598532337644002
2216	42-43	0.0273635327344266
2216	44-45	0.022712507269361026
2216	46-47	0.05784675804654427
2216	48-49	0.06056205107410051
2216	50-51	0.08665619420681026
2216	52-53	0.039674707085950445
2216	54-55	0.05356534748833042
2216	56-57	0.0965721825335919
2216	58-59	0.13617636648093878
2216	60-61	-0.0063819643232818635
2216	62-63	0.019274608254455927
2216	64-65	0.05606726127866324
2216	66-67	0.043897640906294555
2216	68-69	0.060549961289204646
2216	70-71	0.07228782302632908
2216	72-73	0.05094504500628716
2216	74-75	0.083383420858965
2216	76-77	0.07999792662936756
2216	78-79	0.05954617436367471
2216	80-81	0.031658527241319234
2216	82-83	0.03404413877338186
2216	84-85	0.06029484040477229
2216	86-87	0.0656074308065584
2216	88-89	0.0948012812507173
2216	90-91	0.06423942649688996
2216	92-93	0.08001000468356523
2216	94-95	0.0627459482538697
2216	96-97	0.04143912692028451
2216	98-99	0.018606952949266287
2216	100	0.06286289336545536
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70.0
3	521.0
4	139.0
5	169.0
6	337.0
7	763.0
8	1287.0
9	2093.0
10	3165.0
11	4411.0
12	6503.0
13	9614.0
14	10949.0
15	14763.0
16	19909.0
17	30977.0
18	36734.0
19	19267.0
20	11868.0
21	10649.0
22	10562.0
23	10573.0
24	11089.0
25	12548.0
26	15470.0
27	19190.0
28	24066.0
29	30571.0
30	38318.0
31	47537.0
32	60367.0
33	78092.0
34	109755.0
35	195819.0
36	313160.0
37	622765.0
38	1032189.0
39	656441.0
40	6111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49317974708289	20.594087868430204	14.86550662477254	22.04722575971437
2	14.579242324282449	21.878565484172807	40.55440808471066	22.987784106834084
3	16.562300756643268	28.968094042002008	32.09924381666478	22.370361384689947
4	11.16757995763495	18.885561762337762	39.37086550548449	30.575992774542797
5	11.316386137019329	39.21331679910918	35.13087421259208	14.33942285127941
6	26.980856390301167	39.963596757627826	18.453718813698128	14.60182803837288
7	23.7756808288809	36.05815320234414	23.11459288820232	17.051573080572645
8	25.20246141569634	34.939236423019246	20.569987849296787	19.28831431198763
9	26.4107266565291	15.85605422543916	21.790382343387538	35.942836774644206
10-11	23.232262402297795	27.5237142805401	28.842943752908678	20.40107956425342
12-13	25.07381113834583	26.08954611216304	29.232372784839416	19.604269964651717
14-15	22.25386777263841	26.727235253654193	28.60323541577855	22.415661557928846
16-17	19.396799653674776	30.12247575392857	29.091131998835234	21.38959259356142
18-19	19.286264555983223	27.19447167278466	33.8703608417821	19.648902929450013
20-21	20.958712617615618	26.076668148973887	33.89015672308729	19.07446251032321
22-23	20.514667593804027	26.17537010157357	33.99402410336758	19.315938201254827
24-25	20.01962739568203	26.358876639173555	33.99007591961736	19.631420045527047
26-27	19.86604890699599	26.451496159459264	34.006758638809224	19.675696294735523
28-29	19.67719977647516	26.421248312640422	34.233205741736825	19.668346169147593
30-31	19.947234799965965	26.55536034181823	33.90156915817142	19.595835700044383
32-33	19.798750563238272	26.483848087002077	34.04932925238609	19.668072097373567
34-35	20.06007272369781	26.398524081765938	33.81146024177705	19.729942952759206
36-37	19.961647402453767	26.566920784006204	33.54006807284234	19.93136374069769
38-39	20.043742296158655	26.622564091360662	33.411746748289055	19.921946864191625
40-41	20.065226430516574	26.553346127958616	33.38257438726436	19.99885305426045
42-43	20.088864154895102	26.696184567463487	33.201098771901634	20.01385250573978
44-45	20.260454267864567	26.706515582283753	32.845876883350655	20.187153266501028
46-47	20.42967603549148	26.8722582195563	32.2379626118954	20.46010313305682
48-49	20.537451333905178	27.10884015444371	31.632171134480547	20.72153737717057
50-51	20.61375456658774	27.288453694246957	31.39067167107288	20.707120068092426
52-53	20.621437338844828	27.416759850407757	31.250262303847027	20.711540506900388
54-55	20.640470831119316	27.525835532497055	31.14728722057949	20.68640641580414
56-57	20.588775972927	27.41520822538604	31.10806563003729	20.88795017164967
58-59	20.660450619130234	27.107215249893535	31.375809674135404	20.85652445684083
60-61	20.66797689875137	27.285008248597997	31.298276719981445	20.748738132669192
62-63	20.509776782217315	27.145570534476548	31.555223063274383	20.78942962003175
64-65	20.618473871338796	27.020819077161622	31.636577610419124	20.724129441080454
66-67	20.57578762791767	27.078317235828447	31.44085604231344	20.905039093940438
68-69	20.792678168041494	27.121524467550103	31.28031306184041	20.805484302567997
70-71	20.756521711634722	27.170567002500572	31.250619825267485	20.822291460597224
72-73	20.728755995233996	27.274408884660104	31.167383627423785	20.829451492682114
74-75	20.7495039947752	27.258972505989142	30.98687361194713	21.004649887288526
76-77	20.755073318638562	27.151295221209224	30.879562077367172	21.214069382785038
78-79	20.71623949829545	27.30929703670582	30.886570739372605	21.08789272562612
80-81	20.452139968207337	27.3314428292596	31.064364333575757	21.15205286895731
82-83	20.494830780463758	27.324366549623214	31.070078030565444	21.110724639347584
84-85	20.580549604781574	27.203374178679407	30.84365246649501	21.372423750044018
86-87	20.59816957029918	27.29039429725539	30.754257424945656	21.35717870749977
88-89	20.48843083466498	27.19971955629183	30.778783208907278	21.533066400135912
90-91	20.504108703829093	27.173743849664255	30.688430850700687	21.633716595805964
92-93	20.412915406584876	27.261460246572394	30.767765841745177	21.557858505097556
94-95	20.28899749323363	27.17124328914309	30.881447241126892	21.65831197649639
96-97	20.381208489696892	27.28175336070863	30.632559169024997	21.704478980569476
98-99	20.214944808554673	27.248217776244683	30.766384960331152	21.770452454869496
100	20.3120036909226	27.289543392055606	30.630374510787465	21.768078406234327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3085.0
1	1889.0
2	2778.5
3	5008.5
4	3126.5
5	1945.0
6	5422.0
7	14231.0
8	25946.5
9	28864.0
10	17674.5
11	7158.0
12	5064.5
13	5143.5
14	5593.0
15	6021.0
16	6478.5
17	7098.0
18	7871.0
19	8695.5
20	9840.5
21	11680.5
22	14458.5
23	18281.5
24	23223.5
25	28899.5
26	36190.5
27	44226.0
28	53298.0
29	64163.0
30	73507.0
31	80544.0
32	85258.5
33	91424.0
34	97591.5
35	101606.0
36	104351.0
37	107060.5
38	109801.0
39	109678.0
40	108657.0
41	107558.5
42	106185.5
43	105029.0
44	103533.5
45	101728.5
46	101625.0
47	100089.5
48	97047.0
49	97005.0
50	98063.0
51	94740.0
52	89464.5
53	85212.5
54	82093.5
55	78961.5
56	70600.0
57	61218.0
58	56246.5
59	54048.5
60	49135.0
61	40632.0
62	34293.5
63	28434.5
64	21757.0
65	17403.5
66	14133.5
67	10706.5
68	8016.0
69	6029.0
70	4354.0
71	3213.5
72	2506.0
73	1907.5
74	1512.0
75	1113.0
76	650.0
77	483.0
78	391.0
79	210.5
80	82.0
81	36.5
82	25.5
83	19.5
84	10.0
85	3.0
86	3.5
87	2.5
88	0.5
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	1.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005777836162987871
2	0.004196836217891688
3	7.186363386800835E-4
4	0.0
5	0.04538907115103408
6	0.0
7	0.0
8	0.0
9	2.8745453547203338E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.8745453547203338E-5
20-21	0.0
22-23	7.186363386800835E-5
24-25	0.0
26-27	1.1498181418881335E-4
28-29	8.623636064161002E-5
30-31	8.623636064161002E-5
32-33	0.0012504272293033453
34-35	7.904999725480919E-4
36-37	0.0010779545080201252
38-39	7.761272457744902E-4
40-41	1.868454480568217E-4
42-43	5.174181638496601E-4
44-45	1.2935454096241504E-4
46-47	1.1498181418881335E-4
48-49	8.623636064161002E-5
50-51	2.874545354720334E-4
52-53	6.036545244912702E-4
54-55	7.617545190008885E-4
56-57	0.0019690635679834286
58-59	5.605363441704652E-4
60-61	0.001092327234793727
62-63	9.629726938313119E-4
64-65	0.001566627218322582
66-67	3.161999890192367E-4
68-69	2.7308180869843173E-4
70-71	2.299636283776267E-4
72-73	1.7247272128322004E-4
74-75	4.886727103024568E-4
76-77	6.611454315856768E-4
78-79	8.767363331897019E-4
80-81	6.036545244912702E-4
82-83	0.0013222908631713537
84-85	0.0011066999615673286
86-87	0.0018684544805682172
88-89	0.001092327234793727
90-91	0.0010779545080201252
92-93	0.0010204636009257186
94-95	7.617545190008885E-4
96-97	1.7247272128322004E-4
98-99	3.161999890192367E-4
100	1.7247272128322004E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3478811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.15624383364144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.49923591164502	31.358541834003777
2	17.152738399447845	16.17717428758054
3	6.858390916913821	9.702478631532594
4	3.285699364053534	6.197649615013963
5	1.8189623983101664	4.288771718946971
6	1.132583833350124	3.204503964449925
7	0.7330982142944141	2.419911070309216
8	0.49663226264129	1.873544965821258
9	0.3555398500861779	1.5089331476916112
>10	1.5548801271611539	12.35103917809078
>50	0.0708863339078078	2.2901490737694448
>100	0.03719929112927877	3.3616786726085737
>500	0.0024429982743496086	0.8170169965306673
>1k	0.0012825740887931535	0.8891050347033141
>5k	2.442998264367911E-4	0.7175107294730868
>10k+	1.8322486982759334E-4	2.8419910794743815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42646	1.2258786119740337	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33773	0.9708202026496984	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22259	0.6398450505071991	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7487	0.21521721070791142	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6539	0.18796652074516265	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5828	0.16752850327310106	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5059	0.1454232494953017	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3980	0.1144069051178693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.874545354720334E-5	0.0
18-19	0.0	0.0	0.0	1.0060908741521169E-4	0.0
20-21	0.0	0.0	0.0	1.7247272128322004E-4	0.0
22-23	0.0	0.0	0.0	5.174181638496601E-4	0.0
24-25	0.0	0.0	0.0	0.0011354454151145318	0.0
26-27	0.0	0.0	0.0	0.001782218119926607	0.0
28-29	0.0	0.0	0.0	0.003780027141457239	0.0
30-31	0.0	0.0	0.0	0.008954208779953841	0.0
32-33	0.0	0.0	0.0	0.016384908521905904	0.0
34-35	0.0	0.0	0.0	0.028343017197542494	0.0
36-37	0.0	0.0	0.0	0.047602471074168734	0.0
38-39	0.0	0.0	0.0	0.07341588835955733	0.0
40-41	0.0	0.0	0.0	0.10203198736579826	0.0
42-43	0.0	0.0	0.0	0.12954138641047186	0.0
44-45	0.0	0.0	0.0	0.1634322761426246	0.0
46-47	0.0	0.0	0.0	0.20715411098792086	0.0
48-49	0.0	0.0	0.0	0.2565531729087898	0.0
50-51	0.0	0.0	0.0	0.3053773257587147	0.0
52-53	0.0	0.0	0.0	0.3523186514012977	0.0
54-55	0.0	0.0	0.0	0.4059720404471528	0.0
56-57	0.0	0.0	0.0	0.47510485622817683	0.0
58-59	0.0	0.0	0.0	0.5523007717291913	0.0
60-61	0.0	0.0	0.0	0.6383646596495182	0.0
62-63	0.0	0.0	0.0	0.7253771475369026	0.0
64-65	0.0	0.0	0.0	0.817520698882463	0.0
66-67	0.0	0.0	0.0	0.9253017769577019	0.0
68-69	0.0	0.0	0.0	1.0510056453196222	0.0
70-71	0.0	0.0	0.0	1.1929938131160331	0.0
72-73	0.0	0.0	0.0	1.341147880698319	0.0
74-75	0.0	0.0	0.0	1.4918028027392118	0.0
76-77	0.0	0.0	0.0	1.6587420242145952	0.0
78-79	0.0	0.0	0.0	1.8572437536848079	0.0
80-81	0.0	0.0	0.0	2.073337700725909	0.0
82-83	0.0	0.0	0.0	2.308015583485277	0.0
84-85	0.0	1.437272677360167E-5	0.0	2.5452374388835723	2.874545354720334E-5
86-87	0.0	2.874545354720334E-5	0.0	2.795567221099393	2.874545354720334E-5
88	0.0	2.874545354720334E-5	0.0	3.000766641246104	2.874545354720334E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12110	0.0	63.524178	1
GTATCAA	24205	0.0	46.157394	1
GTGGTAT	3760	0.0	37.142277	1
TATCAAC	31280	0.0	35.549976	2
ATCAACG	31350	0.0	35.333584	3
TCAACGC	31650	0.0	35.042732	4
CAACGCA	32810	0.0	33.818123	5
TGGTATC	4135	0.0	33.77193	2
AACGCAG	33700	0.0	32.996372	6
ACGCAGA	37335	0.0	29.620142	7
CGCAGAG	37455	0.0	29.387218	8
GCAGAGT	39955	0.0	27.266142	9
CATGGGT	9050	0.0	26.495085	4
GTACATG	19640	0.0	25.857243	1
TACATGG	19920	0.0	24.996637	2
ACATGGG	19960	0.0	24.356222	3
GAGTACT	27890	0.0	24.029922	12-13
CAGAGTA	39015	0.0	23.32745	10-11
AGAGTAC	38070	0.0	22.283108	10-11
AGTACTT	28430	0.0	22.21794	12-13
>>END_MODULE