FastQCFastQC Report
Wed 25 May 2016
SRR2431420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171465
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181480.572227661348935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.047.3957331
GTATCAA71100.037.519361
TCAACGC81800.031.2252714
ATCAACG82250.031.0573753
TATCAAC89250.029.3599052
CAACGCA87900.029.1653565
AACGCAG94000.027.0473426
GTACATG149900.026.0506421
TACATGG149900.025.1698382
CATGGGT66750.024.100974
CGCAGAG104400.023.9024498
ACATGGG152650.023.8222893
ACGCAGA108150.023.2054777
GTACCTG24300.021.6847151
GTGGTAT20800.021.2621171
ATGGGTG40000.020.6972375
GCAGAGT119000.020.0612649
TACCTGG24850.019.4992772
TGGTATC22900.018.8999212
CAGAGTA116600.017.5321710-11
Produced by FastQC (version 0.11.5)