FastQCFastQC Report
Wed 25 May 2016
SRR2431420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171465
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259350.8177608770710066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212270.669312131775063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136870.4315671148822389No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.13564709054017624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.1347011554597008No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33310.10503032510212158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88800.065.9818041
GTATCAA177800.045.913151
GTGGTAT28650.039.227661
TGGTATC28450.038.014052
ATCAACG230100.035.1471333
TATCAAC231850.034.924052
TCAACGC232900.034.724584
CAACGCA239900.033.7309575
AACGCAG249100.032.5080576
CGCAGAG279500.028.820968
ACGCAGA282000.028.6321187
GCAGAGT302350.026.4562979
CATGGGT65450.023.7073124
GAGTACT213300.023.47696912-13
GTACATG146500.023.431731
TACATGG145300.023.1710642
CAGAGTA297750.022.83211310-11
ACATGGG145550.022.5486873
AGAGTAC286850.022.2087510-11
ATGGGTG38350.021.2108845
Produced by FastQC (version 0.11.5)