##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431420_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3171465
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62146925789816	34.0	31.0	34.0	31.0	34.0
2	32.768147212723456	34.0	31.0	34.0	31.0	34.0
3	32.84542474849951	34.0	31.0	34.0	31.0	34.0
4	36.30871537286396	37.0	37.0	37.0	35.0	37.0
5	36.22592776524414	37.0	37.0	37.0	35.0	37.0
6	36.27452990967897	37.0	37.0	37.0	35.0	37.0
7	36.2278048157555	37.0	37.0	37.0	35.0	37.0
8	36.24602825508085	37.0	37.0	37.0	35.0	37.0
9	38.03842041454028	39.0	39.0	39.0	35.0	39.0
10-11	38.019416894085225	39.0	39.0	39.0	35.0	39.0
12-13	38.00332748430142	39.0	39.0	39.0	35.0	39.0
14-15	39.45909051495129	41.0	39.0	41.0	36.0	41.0
16-17	39.49043706930394	41.0	39.0	41.0	36.0	41.0
18-19	39.57336341406889	41.0	40.0	41.0	37.0	41.0
20-21	39.57315057867579	41.0	40.0	41.0	37.0	41.0
22-23	39.518679853001686	41.0	40.0	41.0	36.0	41.0
24-25	39.47170976189237	41.0	39.0	41.0	36.0	41.0
26-27	39.35400737514051	41.0	39.0	41.0	36.0	41.0
28-29	39.273578141332166	41.0	39.0	41.0	36.0	41.0
30-31	39.08925039374547	41.0	39.0	41.0	35.0	41.0
32-33	38.857717963149526	41.0	38.0	41.0	35.0	41.0
34-35	38.721546824574766	41.0	38.0	41.0	35.0	41.0
36-37	38.5771980772293	41.0	38.0	41.0	35.0	41.0
38-39	38.42825602678889	41.0	38.0	41.0	35.0	41.0
40-41	38.28767194340786	40.5	38.0	41.0	34.5	41.0
42-43	38.116170444889036	40.0	38.0	41.0	34.0	41.0
44-45	37.86977091028909	40.0	38.0	41.0	33.5	41.0
46-47	37.49432328592622	40.0	38.0	41.0	33.0	41.0
48-49	37.1530073641046	40.0	38.0	41.0	32.0	41.0
50-51	36.98565505216044	40.0	37.0	41.0	31.0	41.0
52-53	36.930102176754275	40.0	37.0	41.0	31.0	41.0
54-55	36.802077746404265	40.0	37.0	41.0	31.0	41.0
56-57	36.65876290610176	40.0	36.0	41.0	31.0	41.0
58-59	36.48896219885763	40.0	36.0	41.0	31.0	41.0
60-61	36.34617109127801	40.0	35.5	41.0	31.0	41.0
62-63	36.196091396247475	39.0	35.0	41.0	31.0	41.0
64-65	35.972641034979105	39.0	35.0	41.0	30.0	41.0
66-67	35.715251626614204	39.0	35.0	41.0	30.0	41.0
68-69	35.431741797560434	38.5	35.0	41.0	29.0	41.0
70-71	35.135151420558	37.0	35.0	40.0	29.0	41.0
72-73	34.819494145450136	37.0	35.0	40.0	29.0	41.0
74-75	34.483033393084895	36.5	35.0	39.0	29.0	41.0
76-77	34.1453333081084	36.0	35.0	39.0	29.0	41.0
78-79	33.81783339876051	36.0	35.0	38.5	28.5	40.0
80-81	33.540526696652805	35.0	34.5	37.0	29.0	39.0
82-83	33.3218939827493	35.0	35.0	37.0	29.0	39.0
84-85	33.044227352343476	35.0	34.0	36.5	29.0	39.0
86-87	32.78820340126724	35.0	34.0	36.0	29.0	37.0
88-89	32.544558429621645	35.0	34.0	36.0	28.0	37.0
90-91	32.34998510152248	35.0	34.0	36.0	27.5	37.0
92-93	32.18286832741336	35.0	34.0	35.0	27.0	36.0
94-95	32.038282623330225	35.0	34.0	35.0	27.0	36.0
96-97	31.891744509241	35.0	34.0	35.0	26.5	36.0
98-99	31.773811787297035	35.0	34.0	35.0	26.0	36.0
100	31.668282008472424	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03122647420587299
1101	2	-1.0567115936055416E-4
1101	3	0.04609647341737855
1101	4	0.05379656207131944
1101	5	0.04181935664451686
1101	6	0.007260094050550947
1101	7	0.06022486049160136
1101	8	0.04710795301702575
1101	9	0.04250154526155825
1101	10-11	0.07943227611100667
1101	12-13	0.012426276258935331
1101	14-15	0.058461773797581884
1101	16-17	0.038179642297940575
1101	18-19	-0.002258402440659779
1101	20-21	0.05935420823949045
1101	22-23	0.011912916413571395
1101	24-25	0.04388541693065662
1101	26-27	0.04024517684358386
1101	28-29	-0.042883174127695156
1101	30-31	-0.08174533119264282
1101	32-33	-0.12078343707480599
1101	34-35	-0.12750913697968258
1101	36-37	-0.11952752336310368
1101	38-39	-0.18020700275655344
1101	40-41	-0.2262054345638731
1101	42-43	-0.24919878190901557
1101	44-45	-0.16756334606549217
1101	46-47	-0.14551507391481522
1101	48-49	-0.2498077143138815
1101	50-51	-0.37582109599808433
1101	52-53	-0.32478842746554903
1101	54-55	-0.318271784593513
1101	56-57	-0.2583334112955882
1101	58-59	-0.29217585274742675
1101	60-61	-0.37458161774507204
1101	62-63	-0.403439499245259
1101	64-65	-0.391989041798567
1101	66-67	-0.30450223411721566
1101	68-69	-0.3433225155139752
1101	70-71	-0.33397557455932514
1101	72-73	-0.31168697192313033
1101	74-75	-0.3370222713611355
1101	76-77	-0.38502439767085406
1101	78-79	-0.35586005670651133
1101	80-81	-0.3692400375110836
1101	82-83	-0.35809266756773894
1101	84-85	-0.3147338506338784
1101	86-87	-0.30461379336761496
1101	88-89	-0.29986617107023505
1101	90-91	-0.2781927196798648
1101	92-93	-0.2598336441626685
1101	94-95	-0.27341961907728773
1101	96-97	-0.2923512204350338
1101	98-99	-0.2779151482113278
1101	100	-0.21312796458942884
1102	1	-0.022452534306140137
1102	2	0.005767344364130622
1102	3	0.030091914621145577
1102	4	0.052577356750688864
1102	5	0.05096229554917642
1102	6	-4.607275616237416E-4
1102	7	-0.010596149644577224
1102	8	0.03989921356888004
1102	9	0.014331872622612707
1102	10-11	-0.002053395591104845
1102	12-13	0.017000884732766508
1102	14-15	-0.003919625654106085
1102	16-17	0.0013644251088038573
1102	18-19	0.01780404440972916
1102	20-21	0.04069710599954135
1102	22-23	0.12706475767690506
1102	24-25	0.12084134005574043
1102	26-27	0.0764498777765823
1102	28-29	0.03920954685157341
1102	30-31	0.06974119928953115
1102	32-33	0.06194811694557956
1102	34-35	-0.016539784183599693
1102	36-37	-0.02756845957674159
1102	38-39	-0.0325009551598896
1102	40-41	-0.014495792740753188
1102	42-43	0.010121593699736309
1102	44-45	0.060745160340758275
1102	46-47	-0.036677046440324546
1102	48-49	-0.034809549235930604
1102	50-51	-0.05114252125815
1102	52-53	-0.07927061049664275
1102	54-55	-0.0445846697942045
1102	56-57	-0.044609478116640844
1102	58-59	-0.07351437930291382
1102	60-61	-0.10662701211885661
1102	62-63	-0.05649008232787622
1102	64-65	-0.1062531912073652
1102	66-67	-0.1300646308135427
1102	68-69	-0.151985662010091
1102	70-71	-0.07510602334429706
1102	72-73	-0.06998236331015306
1102	74-75	-0.14697047235277694
1102	76-77	-0.13975381378334362
1102	78-79	-0.1351527611379808
1102	80-81	-0.09842095763887215
1102	82-83	-0.08898625188099629
1102	84-85	-0.09619088696374689
1102	86-87	-0.11187154840221325
1102	88-89	-0.11718345928736795
1102	90-91	-0.10670464873460617
1102	92-93	-0.08958975015683279
1102	94-95	-0.08430266283745169
1102	96-97	-0.08908831065861733
1102	98-99	-0.1427471723774829
1102	100	-0.1347559211084537
1103	1	-0.07483640059806618
1103	2	-0.051459169533480065
1103	3	-0.06476565616159036
1103	4	-0.020916999943906944
1103	5	-0.053144672333083065
1103	6	-0.05349432789306263
1103	7	-0.10111652019256212
1103	8	-0.0556688533748968
1103	9	-0.049647509761271635
1103	10-11	0.011797026200852656
1103	12-13	-0.011850933653178686
1103	14-15	-0.047752181512720426
1103	16-17	-0.034576065965055136
1103	18-19	-0.0031876794153973265
1103	20-21	-0.04152876755877344
1103	22-23	0.0032081372137611197
1103	24-25	-0.0036440281449543477
1103	26-27	-0.005804694199142091
1103	28-29	-0.030743546660211507
1103	30-31	-0.12258697057220047
1103	32-33	-0.07786442630069956
1103	34-35	-0.002758374139673947
1103	36-37	-0.0085547998778992
1103	38-39	-0.014669165464894718
1103	40-41	-0.008996376711486676
1103	42-43	0.07162396145939454
1103	44-45	-0.020760939147962176
1103	46-47	-0.0938320479949013
1103	48-49	0.01695533799799165
1103	50-51	0.019765963134489084
1103	52-53	0.09041144656104905
1103	54-55	0.12176960945765813
1103	56-57	0.18912660525422353
1103	58-59	0.02107277666895868
1103	60-61	0.03793465180604727
1103	62-63	0.10929938963432306
1103	64-65	0.0710796349024676
1103	66-67	0.12267034238661978
1103	68-69	0.11349208624663731
1103	70-71	0.10606921237067723
1103	72-73	0.05691217626907985
1103	74-75	0.15647011734267835
1103	76-77	0.09603820270955765
1103	78-79	0.14517284250565154
1103	80-81	0.17307843371163045
1103	82-83	0.14918714900486663
1103	84-85	0.14689901087550794
1103	86-87	0.180100985436205
1103	88-89	0.15161951944009644
1103	90-91	0.1630507295077308
1103	92-93	0.17873514752442787
1103	94-95	0.13591020276589916
1103	96-97	0.15279508514753104
1103	98-99	0.13280996018882618
1103	100	0.17487756738329452
1104	1	-0.12393513792075339
1104	2	-0.05177604486650722
1104	3	-0.07965594291074751
1104	4	-0.04415983324022932
1104	5	-0.04610832759776429
1104	6	-0.0848433514346354
1104	7	-0.07367310941349814
1104	8	-0.12187057315546923
1104	9	-0.04874392713671938
1104	10-11	-0.05951433134021045
1104	12-13	-0.05020638197562732
1104	14-15	-0.08744542004490796
1104	16-17	-0.06775117337308245
1104	18-19	-0.06304001661494141
1104	20-21	-0.04082505022998362
1104	22-23	-0.07321718497351526
1104	24-25	-0.019053357057870812
1104	26-27	-0.02940389716837899
1104	28-29	-0.03801630506642795
1104	30-31	-0.05046906312041699
1104	32-33	-0.04339576418480817
1104	34-35	-0.027944163247639153
1104	36-37	-0.0629944331936656
1104	38-39	-0.11537554336808853
1104	40-41	-0.1617966174704506
1104	42-43	-0.09513066987337027
1104	44-45	-0.02481067402954551
1104	46-47	0.05226211829523919
1104	48-49	0.1347513954870152
1104	50-51	4.4340231980299905E-5
1104	52-53	0.10704859834341107
1104	54-55	0.14105136299898646
1104	56-57	0.07698265731531251
1104	58-59	0.12941454282638176
1104	60-61	0.10370455759777286
1104	62-63	0.04693115251068036
1104	64-65	0.07367406319934133
1104	66-67	0.06643182934760006
1104	68-69	0.0972914896151309
1104	70-71	0.17103938079515757
1104	72-73	0.09726498868956668
1104	74-75	0.15954851241054513
1104	76-77	0.12918062832117982
1104	78-79	0.08646874109670932
1104	80-81	0.0779443481262021
1104	82-83	0.08713132223642361
1104	84-85	0.03831203918364423
1104	86-87	0.06596404631567765
1104	88-89	0.10223806156315618
1104	90-91	0.07910946873055735
1104	92-93	0.05684415300724055
1104	94-95	-0.0016300786989376093
1104	96-97	0.02646558046362557
1104	98-99	-0.02083494389976792
1104	100	2.3819949895198533E-4
1105	1	-0.17119433309851928
1105	2	-0.16602348849293236
1105	3	-0.09201399474979866
1105	4	-0.05618594715012648
1105	5	-0.09361713754418588
1105	6	-0.04595772151925104
1105	7	-0.017447348567344534
1105	8	-0.06280838035654313
1105	9	-0.023343469429065067
1105	10-11	-0.09453629132832475
1105	12-13	-0.07519793758051208
1105	14-15	-0.11836020686258308
1105	16-17	-0.1342874425859648
1105	18-19	-0.15043499073354383
1105	20-21	-0.05977663992442217
1105	22-23	-0.09211659865930244
1105	24-25	-0.10847496319778571
1105	26-27	-0.04784572178529345
1105	28-29	-0.0638730063951769
1105	30-31	-0.12832684208441947
1105	32-33	-0.09009782996251658
1105	34-35	-0.07260878781343649
1105	36-37	-0.12491387817958355
1105	38-39	-0.06370649487879376
1105	40-41	-0.08780873398021072
1105	42-43	-0.13230593921149136
1105	44-45	-0.09567164567270936
1105	46-47	-0.16408941865764604
1105	48-49	-0.24081240414142258
1105	50-51	-0.26814919225985534
1105	52-53	-0.28445125484314815
1105	54-55	-0.17846883572957495
1105	56-57	-0.2013822095131772
1105	58-59	-0.17113044105698094
1105	60-61	-0.15213500500673405
1105	62-63	-0.18856843231606746
1105	64-65	-0.18214342493995872
1105	66-67	-0.21662609614706696
1105	68-69	-0.09938592938367208
1105	70-71	-0.13317507245181304
1105	72-73	-0.06272330089786493
1105	74-75	-0.061568942626180956
1105	76-77	-0.09666482141549437
1105	78-79	-0.07082550596015835
1105	80-81	-0.05479278613206162
1105	82-83	-0.08691507226911455
1105	84-85	-0.06385729695782771
1105	86-87	-0.06830575819669349
1105	88-89	-0.06736087550574155
1105	90-91	-0.06211600432659736
1105	92-93	-0.1012028606779296
1105	94-95	-0.12067420327444722
1105	96-97	-0.07677409358956311
1105	98-99	-0.13325002448114276
1105	100	-0.11307413511929099
1106	1	-0.17750074636838065
1106	2	-0.09746949824303641
1106	3	-0.09564997533529151
1106	4	-0.02278308710725696
1106	5	-0.056771433277155836
1106	6	-0.09295100749764629
1106	7	-0.0087480887076552
1106	8	-0.013151401603771262
1106	9	-0.04359984489293822
1106	10-11	-0.07112726716910345
1106	12-13	-0.054885902065208825
1106	14-15	-0.06851366256173463
1106	16-17	-0.08076280442092809
1106	18-19	-0.07200712160742029
1106	20-21	-0.07653547076875356
1106	22-23	-0.11521668052019152
1106	24-25	-0.07421711655884877
1106	26-27	-0.10383571636658218
1106	28-29	-0.13777029463032875
1106	30-31	-0.1681998818195396
1106	32-33	-0.16929170548218764
1106	34-35	-0.14528483560162897
1106	36-37	-0.11036953180355624
1106	38-39	-0.13473563896378948
1106	40-41	-0.2400001643855063
1106	42-43	-0.23105651165748498
1106	44-45	-0.2771999849856712
1106	46-47	-0.2670090271536836
1106	48-49	-0.14953005368931116
1106	50-51	-0.1473953355583788
1106	52-53	-0.14069433081309768
1106	54-55	-0.1760755427880909
1106	56-57	-0.14527455663550626
1106	58-59	-0.12855251028969406
1106	60-61	-0.17604307263436425
1106	62-63	-0.16424338297188257
1106	64-65	-0.14407790855126734
1106	66-67	-0.17907339317534365
1106	68-69	-0.18235426608993066
1106	70-71	-0.16417727475087673
1106	72-73	-0.1006020860714898
1106	74-75	-0.11327822397323928
1106	76-77	-0.0876101094149675
1106	78-79	-0.13034413355379826
1106	80-81	-0.10592763521610493
1106	82-83	-0.140601267415839
1106	84-85	-0.0767159293542079
1106	86-87	-0.10488445490342002
1106	88-89	-0.12788379432419106
1106	90-91	-0.16711849050531669
1106	92-93	-0.10250424356463128
1106	94-95	-0.16861986978013022
1106	96-97	-0.1691458545271871
1106	98-99	-0.1962702584943905
1106	100	-0.20898265266939475
1107	1	-0.17686267105457176
1107	2	-0.18006974231555972
1107	3	-0.13503805064910068
1107	4	-0.1741760438715545
1107	5	-0.2216685741418729
1107	6	-0.16893252772079848
1107	7	-0.14240743319859206
1107	8	-0.14748194553683192
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2216	36-37	-0.02073261754100031
2216	38-39	-0.03302365674525021
2216	40-41	-0.032712303825718436
2216	42-43	0.05442406645586573
2216	44-45	0.009948009936998403
2216	46-47	0.08043837487370809
2216	48-49	0.12647456585349914
2216	50-51	0.11020708395992074
2216	52-53	0.065720776032137
2216	54-55	0.072735738021386
2216	56-57	0.08623759991412783
2216	58-59	0.12539479424484767
2216	60-61	0.04857587673948416
2216	62-63	0.07165021032184171
2216	64-65	0.04068370793419973
2216	66-67	0.011141002473003425
2216	68-69	0.0068896517817762515
2216	70-71	0.09522820380801988
2216	72-73	0.05425113130828407
2216	74-75	0.014596273822959915
2216	76-77	0.028379277351127996
2216	78-79	0.10969094075270647
2216	80-81	0.13909024874967457
2216	82-83	0.13909825421408328
2216	84-85	0.1418480587123554
2216	86-87	0.06444437038091166
2216	88-89	0.0968641693082759
2216	90-91	0.046613859627356646
2216	92-93	0.022930986884588833
2216	94-95	0.04158805588792802
2216	96-97	0.07856953724379778
2216	98-99	0.08869000449740128
2216	100	0.06607231711553041
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44.0
3	250.0
4	69.0
5	101.0
6	255.0
7	574.0
8	1024.0
9	1693.0
10	2650.0
11	4017.0
12	6020.0
13	9029.0
14	10592.0
15	13969.0
16	18075.0
17	27758.0
18	32454.0
19	16771.0
20	10381.0
21	9162.0
22	8551.0
23	8694.0
24	9275.0
25	10467.0
26	13157.0
27	16695.0
28	20898.0
29	26337.0
30	32917.0
31	42106.0
32	52949.0
33	68496.0
34	94366.0
35	155453.0
36	248024.0
37	515224.0
38	993551.0
39	682142.0
40	7275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12038783334947	21.536241599945004	15.17971253688204	22.163658029823484
2	14.142799978936289	22.397851249207353	40.850135164068334	22.60921360778802
3	16.088194887418332	28.582743878079015	32.929772080805165	22.399289153697495
4	10.834267444225302	19.12160468427052	40.55633595199695	29.487791919507234
5	10.937283624735777	39.26281864239594	36.045063150427005	13.754834582441283
6	26.287630479920164	41.02621343763844	18.792671525619863	13.89348455682153
7	23.175567127494705	35.978924566407	23.989449670735763	16.856058635362523
8	24.35300405333182	35.37980081760322	21.570346827097257	18.6968483019677
9	25.335152893160036	15.977168896499816	22.777185166593462	35.91049304374669
10-11	22.145885261227853	27.877605459937282	30.1887455797242	19.787763699110663
12-13	23.97297841423103	26.431873981247467	30.182649056926223	19.412498547595284
14-15	21.312204503629157	27.47806257960636	29.10693151381641	22.102801402948067
16-17	18.71600348734733	30.84618622623929	29.686895488362637	20.750914798050744
18-19	18.670730615261597	28.19303640211587	33.98873517088638	19.14749781173616
20-21	20.214932215868693	27.207930719714707	33.941112388123464	18.636024676293133
22-23	19.747491970189788	27.272708640663843	34.06736353737005	18.912435851776316
24-25	19.41949846829438	27.408142200551193	33.937381294982174	19.234978036172247
26-27	19.208998129882808	27.41110637091635	33.99462203358139	19.385273465619452
28-29	19.160961625293556	27.33978692490282	34.2116290772968	19.28762237250683
30-31	19.478877811458336	27.68070639942727	33.8145622675462	19.025853521568198
32-33	19.18440944027808	27.583517477278402	34.07031902307853	19.16175405936498
34-35	19.46927014949657	27.49113652960607	33.706018457207506	19.333574863689854
36-37	19.72524101084872	27.554349231294108	33.29908489345829	19.42132486439888
38-39	19.539808197447307	27.634825647730516	33.422908878731214	19.40245727609096
40-41	19.53315837716434	27.5564637244475	33.34915674510896	19.5612211532792
42-43	19.491094139559465	27.687263653135425	33.09518261514997	19.726459592155138
44-45	19.830986412886677	27.799301331468367	32.68134150159816	19.688370754046794
46-47	19.965867455367558	28.18176801851513	32.03686945444685	19.815495071670462
48-49	20.11291963730258	28.152941595650454	31.560048545480175	20.174090221566793
50-51	20.08452899866812	28.291532343504056	31.289540198571252	20.33439845925657
52-53	20.02713276371872	28.498246460404058	31.258315419560656	20.216305356316568
54-55	20.185036053068707	28.720822365641308	31.0654857925558	20.02865578873419
56-57	20.089005094451444	28.441253626365587	31.00703587053191	20.46270540865106
58-59	19.978678512357998	28.18502334185783	31.40659544053766	20.429702705246513
60-61	20.15193461624567	28.411631346112003	31.146391375499384	20.290042662142945
62-63	19.96897940573711	28.28152434783993	31.389811226219997	20.35968502020296
64-65	20.01064192065384	28.127494939175513	31.50240270919651	20.35946043097414
66-67	19.93264292489002	28.222368527263185	31.31999939459983	20.524989153246963
68-69	20.37032482526181	28.371043106658483	30.94466931465928	20.313962753420427
70-71	20.292407959174632	28.167315395325087	30.969526806191283	20.570749839309
72-73	20.149590456632104	28.36040131687025	31.08331212858183	20.406696097915816
74-75	20.20191382369968	28.464359542569145	30.829601078622666	20.504125555108512
76-77	20.25212103095963	28.45139120775241	30.700612621407288	20.59587513988068
78-79	20.308638393969186	28.455947224073814	30.84752191902369	20.387892462933312
80-81	20.214712343949657	28.404130284152135	30.847600586797373	20.533556785100835
82-83	20.033801786575772	28.415036749983447	31.08730131202644	20.463860151414345
84-85	20.088593852822015	28.465863603241303	30.736599379650688	20.70894316428599
86-87	20.213185260861067	28.44555435735412	30.64652426988542	20.6947361118994
88-89	20.08875777426693	28.279326066498804	30.790976529435127	20.84093962979914
90-91	19.919881870770457	28.35628083376584	30.714750577182244	21.009086718281463
92-93	19.973586139549813	28.42283681140529	30.709636408181645	20.893940640863253
94-95	19.84668510094684	28.28975674033915	30.825303217317394	21.038254941396612
96-97	19.870286932381177	28.351818239004324	30.671537396875227	21.10635743173928
98-99	19.82384445516156	28.276185862119906	30.661397587261508	21.238572095457027
100	19.89121729424303	28.36059732741387	30.56642681919368	21.181758559149415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1169.0
1	734.0
2	1483.0
3	2807.0
4	1804.5
5	1046.5
6	2814.5
7	7408.0
8	13418.5
9	15073.0
10	9685.0
11	4699.0
12	3983.0
13	4262.5
14	4681.5
15	5114.0
16	5557.0
17	6104.5
18	6908.5
19	7769.0
20	9083.5
21	11286.5
22	15079.0
23	20920.0
24	27001.5
25	32570.0
26	40394.0
27	50559.0
28	63079.5
29	74470.0
30	80081.5
31	85803.0
32	93070.5
33	100246.0
34	106850.5
35	110054.0
36	110524.5
37	110575.0
38	110081.5
39	108853.0
40	108444.5
41	106120.5
42	102263.0
43	99040.5
44	95771.5
45	92880.5
46	90232.0
47	87312.5
48	83414.5
49	80591.0
50	78866.5
51	75150.5
52	69489.5
53	64791.0
54	62050.0
55	59318.5
56	54123.0
57	48398.0
58	43796.0
59	39603.5
60	34598.0
61	28389.0
62	23602.0
63	18955.0
64	14296.0
65	11186.5
66	8630.0
67	6421.0
68	4847.0
69	3527.0
70	2488.0
71	1787.0
72	1314.5
73	959.5
74	754.5
75	561.0
76	319.5
77	259.0
78	203.5
79	93.5
80	40.5
81	17.5
82	14.0
83	12.0
84	5.0
85	1.0
86	0.5
87	1.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006495420885931264
2	0.00422517669279024
3	0.0010720597578721507
4	0.0
5	0.042125642250505684
6	0.0
7	0.0
8	0.0
9	6.30623386983618E-5
10-11	0.0
12-13	1.576558467459045E-5
14-15	1.576558467459045E-5
16-17	0.0
18-19	3.15311693491809E-5
20-21	0.0
22-23	4.729675402377135E-5
24-25	1.576558467459045E-5
26-27	1.891870160950854E-4
28-29	3.15311693491809E-5
30-31	4.729675402377135E-5
32-33	0.0010405285885229697
34-35	6.621545563327989E-4
36-37	9.774662498246078E-4
38-39	8.355759877532938E-4
40-41	1.261246773967236E-4
42-43	4.4143637088853263E-4
44-45	1.261246773967236E-4
46-47	1.576558467459045E-4
48-49	1.891870160950854E-4
50-51	2.837805241426281E-4
52-53	7.094513103565702E-4
54-55	9.144039111262461E-4
56-57	0.002317540947164796
58-59	5.202642942614849E-4
60-61	0.0011035909272213314
62-63	7.409824797057512E-4
64-65	0.0014189026207131405
66-67	2.837805241426281E-4
68-69	1.891870160950854E-4
70-71	1.7342143142049494E-4
72-73	1.891870160950854E-4
74-75	4.099052015393517E-4
76-77	3.783740321901708E-4
78-79	5.990922176344371E-4
80-81	3.9413961686476126E-4
82-83	0.0011035909272213314
84-85	8.198104030787034E-4
86-87	0.0014189026207131405
88-89	7.725136490549319E-4
90-91	7.252168950311606E-4
92-93	7.567480643803416E-4
94-95	5.833266329598467E-4
96-97	1.1035909272213316E-4
98-99	2.0495260076967584E-4
100	1.576558467459045E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3171465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.22775926086966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39973469885513	46.77784563735812
2	14.935572041410023	18.28943218749657
3	4.397467258610365	8.077412000032558
4	1.6260205092654467	3.9823036917816586
5	0.8211666413295189	2.513909671419034
6	0.459731015898018	1.6888979979695333
7	0.2741663731185123	1.1750614883508226
8	0.20061026788776443	0.9826333749992487
9	0.13968385952752943	0.7697276751402637
>10	0.6727534024667428	7.323275073456151
>50	0.043483276601819804	1.853864967504597
>100	0.027336161146755668	3.1363687521581856
>500	0.0013440191131146428	0.6085440057451933
>1k	7.753956411374673E-4	0.8948227031799394
>5k	0.0	0.0
>10k+	1.5507912822749348E-4	1.925900773408182
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25935	0.8177608770710066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21227	0.669312131775063	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13687	0.4315671148822389	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4302	0.13564709054017624	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4272	0.1347011554597008	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3331	0.10503032510212158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.45935080475427E-5	0.0
5	0.0	0.0	0.0	9.45935080475427E-5	0.0
6	0.0	0.0	0.0	9.45935080475427E-5	0.0
7	0.0	0.0	0.0	9.45935080475427E-5	0.0
8	0.0	0.0	0.0	1.261246773967236E-4	0.0
9	0.0	0.0	0.0	2.207181854442663E-4	0.0
10-11	0.0	0.0	0.0	2.3648377011885674E-4	0.0
12-13	0.0	0.0	0.0	2.522493547934472E-4	0.0
14-15	0.0	0.0	0.0	2.522493547934472E-4	0.0
16-17	0.0	0.0	0.0	2.837805241426281E-4	0.0
18-19	0.0	0.0	0.0	3.15311693491809E-4	0.0
20-21	0.0	0.0	0.0	4.256707862139421E-4	0.0
22-23	0.0	0.0	0.0	5.360298789360753E-4	0.0
24-25	0.0	3.15311693491809E-5	0.0	6.621545563327989E-4	0.0
26-27	0.0	6.30623386983618E-5	0.0	0.0013873714513639595	0.0
28-29	0.0	6.30623386983618E-5	0.0	0.0026959149793549667	0.0
30-31	0.0	1.261246773967236E-4	0.0	0.005675610482852562	0.0
32-33	0.0	1.261246773967236E-4	0.0	0.010941315764165772	0.0
34-35	0.0	1.261246773967236E-4	0.0	0.020574088000340537	0.0
36-37	0.0	1.261246773967236E-4	0.0	0.03575634604197114	0.0
38-39	0.0	1.261246773967236E-4	0.0	0.054738109990178044	0.0
40-41	0.0	1.261246773967236E-4	0.0	0.07518607331312185	0.0
42-43	0.0	1.261246773967236E-4	0.0	0.09628042560772387	0.0
44-45	0.0	1.261246773967236E-4	0.0	0.12434316632849488	0.0
46-47	0.0	1.261246773967236E-4	0.0	0.1590116870279193	0.0
48-49	0.0	1.261246773967236E-4	0.0	0.1982364616983003	0.0
50-51	0.0	1.261246773967236E-4	0.0	0.23999949550129043	0.0
52-53	0.0	1.261246773967236E-4	0.0	0.2799021903126788	0.0
54-55	0.0	1.261246773967236E-4	0.0	0.32606382223987973	0.0
56-57	0.0	1.261246773967236E-4	0.0	0.38307217642319874	0.0
58-59	0.0	1.576558467459045E-4	0.0	0.44864124308482045	0.0
60-61	0.0	1.576558467459045E-4	0.0	0.5187665637173987	0.0
62-63	0.0	1.576558467459045E-4	0.0	0.5875202784832877	0.0
64-65	0.0	1.576558467459045E-4	0.0	0.6625486959496636	0.0
66-67	0.0	1.576558467459045E-4	0.0	0.7515927181917506	0.0
68-69	0.0	1.7342143142049494E-4	0.0	0.8587671628096163	0.0
70-71	0.0	2.0495260076967584E-4	0.0	0.9798153219411219	0.0
72-73	0.0	2.207181854442663E-4	0.0	1.1080525876842406	0.0
74-75	0.0	2.207181854442663E-4	0.0	1.2408776385676652	0.0
76-77	0.0	2.207181854442663E-4	0.0	1.3889322442467442	0.0
78-79	0.0	2.207181854442663E-4	0.0	1.5605879301836847	0.0
80-81	0.0	2.207181854442663E-4	0.0	1.7557658684551147	0.0
82-83	0.0	2.207181854442663E-4	0.0	1.963351321865447	0.0
84-85	0.0	2.207181854442663E-4	0.0	2.1729232389447777	0.0
86-87	0.0	2.207181854442663E-4	0.0	2.3965895887231925	0.0
88	0.0	2.207181854442663E-4	0.0	2.581236116431996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8880	0.0	65.981804	1
GTATCAA	17780	0.0	45.91315	1
GTGGTAT	2865	0.0	39.22766	1
TGGTATC	2845	0.0	38.01405	2
ATCAACG	23010	0.0	35.147133	3
TATCAAC	23185	0.0	34.92405	2
TCAACGC	23290	0.0	34.72458	4
CAACGCA	23990	0.0	33.730957	5
AACGCAG	24910	0.0	32.508057	6
CGCAGAG	27950	0.0	28.82096	8
ACGCAGA	28200	0.0	28.632118	7
GCAGAGT	30235	0.0	26.456297	9
CATGGGT	6545	0.0	23.707312	4
GAGTACT	21330	0.0	23.476969	12-13
GTACATG	14650	0.0	23.43173	1
TACATGG	14530	0.0	23.171064	2
CAGAGTA	29775	0.0	22.832113	10-11
ACATGGG	14555	0.0	22.548687	3
AGAGTAC	28685	0.0	22.20875	10-11
ATGGGTG	3835	0.0	21.210884	5
>>END_MODULE