FastQCFastQC Report
Wed 25 May 2016
SRR2431419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231120
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295590.914822105028597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.050.5023541
GTATCAA95050.039.6487121
ATCAACG112850.032.642393
TCAACGC112950.032.5683064
TATCAAC121600.030.758282
CAACGCA123400.029.847955
AACGCAG131550.028.0080286
GTACATG204450.026.1880931
TACATGG202900.025.6217462
CATGGGT103050.025.4262224
CGCAGAG144150.025.1360538
ACGCAGA148450.024.661227
TACCTGG25050.024.6032282
ACATGGG208700.024.0526943
GTGGTAT25400.023.709641
ATGGGTG56400.022.8527975
GCAGAGT155350.022.5978289
TGGTATC26500.022.1918052
GTACCTG30400.021.202921
ATGGGTA41900.020.6572115
Produced by FastQC (version 0.11.5)