##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431419_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3231120
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.453070761841094	31.0	31.0	34.0	26.0	34.0
2	30.538704226398277	33.0	31.0	34.0	26.0	34.0
3	30.658899081433063	34.0	31.0	34.0	26.0	34.0
4	33.79723377652331	37.0	35.0	37.0	28.0	37.0
5	33.982792344450225	37.0	35.0	37.0	30.0	37.0
6	33.98429213399688	37.0	35.0	37.0	30.0	37.0
7	33.90962885934289	37.0	35.0	37.0	29.0	37.0
8	33.82202301369185	37.0	35.0	37.0	28.0	37.0
9	35.17946749114858	39.0	37.0	39.0	27.0	39.0
10-11	35.225245580479836	39.0	37.0	39.0	27.0	39.0
12-13	35.14512862413034	39.0	37.0	39.0	27.0	39.0
14-15	36.478011339721206	40.0	38.0	41.0	27.0	41.0
16-17	36.51968094654485	40.0	38.0	41.0	27.0	41.0
18-19	36.56294117829112	40.5	38.0	41.0	27.0	41.0
20-21	36.65482897571121	40.5	38.0	41.0	27.5	41.0
22-23	36.656456120478346	40.0	38.0	41.0	28.0	41.0
24-25	36.7301729740771	40.0	38.0	41.0	29.0	41.0
26-27	36.782528194557926	40.0	38.0	41.0	29.5	41.0
28-29	36.75439971279309	40.0	38.0	41.0	30.0	41.0
30-31	36.687212638342125	40.0	37.0	41.0	30.0	41.0
32-33	36.623838947485694	40.0	37.0	41.0	30.0	41.0
34-35	36.53865470796504	40.0	37.0	41.0	29.0	41.0
36-37	36.40982182648741	40.0	37.0	41.0	28.5	41.0
38-39	36.28604446755305	40.0	37.0	41.0	28.0	41.0
40-41	36.21289738542673	40.0	36.0	41.0	28.0	41.0
42-43	36.05736648592438	40.0	36.0	41.0	27.0	41.0
44-45	35.92658985119711	40.0	36.0	41.0	27.0	41.0
46-47	35.80052071727451	40.0	35.0	41.0	27.0	41.0
48-49	35.66494296095472	40.0	35.0	41.0	27.0	41.0
50-51	35.52490746242789	40.0	35.0	41.0	27.0	41.0
52-53	35.358865192255315	40.0	35.0	41.0	26.0	41.0
54-55	35.18839628364159	39.0	35.0	41.0	26.0	41.0
56-57	34.993813754982796	39.0	34.5	41.0	26.0	41.0
58-59	34.7654270036396	39.0	34.0	41.0	25.5	41.0
60-61	34.5193186882567	38.5	34.0	41.0	25.0	41.0
62-63	34.41028327638713	38.0	34.0	41.0	25.0	41.0
64-65	34.160642439773206	38.0	34.0	40.0	24.0	41.0
66-67	33.90441333036223	37.0	34.0	40.0	23.0	41.0
68-69	33.618633631681895	37.0	33.0	40.0	23.0	41.0
70-71	33.31398168436951	36.0	33.0	39.5	22.5	41.0
72-73	32.99251931218896	36.0	33.0	39.0	22.0	41.0
74-75	32.718473006264084	35.5	33.0	39.0	21.0	41.0
76-77	32.466401433558644	35.0	33.0	38.5	20.5	40.0
78-79	32.128104496273735	35.0	32.5	37.0	19.5	39.5
80-81	31.811528355492833	35.0	32.0	37.0	18.5	39.0
82-83	31.490311718537225	35.0	32.0	36.5	17.5	39.0
84-85	31.190577106390354	35.0	32.0	36.0	14.0	37.5
86-87	30.911122149595187	35.0	32.0	36.0	9.5	37.0
88-89	30.645137289856148	35.0	31.5	35.5	6.0	37.0
90-91	30.41202586100176	35.0	31.0	35.0	2.0	36.0
92-93	30.186070155240287	35.0	31.0	35.0	2.0	36.0
94-95	29.973082708163115	35.0	31.0	35.0	2.0	36.0
96-97	29.781433682438287	35.0	31.0	35.0	2.0	36.0
98-99	29.569790351333282	35.0	31.0	35.0	2.0	35.5
100	29.39975333630444	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21711364418585077
1101	2	0.22320635054319382
1101	3	0.23131307965622128
1101	4	0.26770995559868993
1101	5	0.2600843403258324
1101	6	0.2473888109199791
1101	7	0.3180386880872845
1101	8	0.26122689159178947
1101	9	0.2110914660156169
1101	10-11	0.17623791341407014
1101	12-13	0.1459185077254972
1101	14-15	0.21794893903437185
1101	16-17	0.2249836048549554
1101	18-19	0.1425853086528477
1101	20-21	0.11525889297284664
1101	22-23	0.09438257052831744
1101	24-25	0.2194470497611718
1101	26-27	0.2046771471338218
1101	28-29	0.21230973258444408
1101	30-31	0.22182747085443566
1101	32-33	0.19390205033104735
1101	34-35	0.2245913255570997
1101	36-37	0.252764988439111
1101	38-39	0.3327229692953395
1101	40-41	0.25096703515278307
1101	42-43	0.25271661375821
1101	44-45	0.22013607291606974
1101	46-47	0.301292645272639
1101	48-49	0.1957676641145767
1101	50-51	0.13445938480570163
1101	52-53	0.14417940420063502
1101	54-55	0.16211758739018478
1101	56-57	0.08001986356595125
1101	58-59	0.06406628166774908
1101	60-61	0.07486074949677857
1101	62-63	0.09479218042095994
1101	64-65	0.11175679272507466
1101	66-67	0.14542887047110753
1101	68-69	0.15007586599215728
1101	70-71	0.14842066091682682
1101	72-73	0.1583833727852877
1101	74-75	0.1321593260756515
1101	76-77	0.1860650011726861
1101	78-79	0.14986306015864415
1101	80-81	0.16304177503772976
1101	82-83	0.22559729526687988
1101	84-85	0.18705966329547863
1101	86-87	0.14777006859526765
1101	88-89	0.06785959526963126
1101	90-91	-0.008993629690596805
1101	92-93	0.045414607116736505
1101	94-95	0.07602465549495818
1101	96-97	0.02238146661110818
1101	98-99	-0.005788426039664785
1101	100	0.0078562698235487
1102	1	0.2365227206202576
1102	2	0.173415385280137
1102	3	0.2837336509781707
1102	4	0.3219757826674865
1102	5	0.2671232155985166
1102	6	0.3695918080920677
1102	7	0.24068760064493233
1102	8	0.26401038796253573
1102	9	0.24202794068511935
1102	10-11	0.2514392012755877
1102	12-13	0.18106383244452928
1102	14-15	0.2342883012059076
1102	16-17	0.21511867894650294
1102	18-19	0.21649823520105116
1102	20-21	0.13602444610451414
1102	22-23	0.19433311428519318
1102	24-25	0.2837151521027508
1102	26-27	0.3160294017244709
1102	28-29	0.30796795995654946
1102	30-31	0.40803815159415535
1102	32-33	0.44942075302572704
1102	34-35	0.4493964160753592
1102	36-37	0.3988974055070855
1102	38-39	0.3117080167547712
1102	40-41	0.33267469877607425
1102	42-43	0.36113481365568134
1102	44-45	0.280783873541246
1102	46-47	0.2712408613968975
1102	48-49	0.26063942774133864
1102	50-51	0.2381755764179374
1102	52-53	0.25050173514619445
1102	54-55	0.2230655764631706
1102	56-57	0.14529215612223823
1102	58-59	0.16974727604763729
1102	60-61	0.19301590310863048
1102	62-63	0.22587185923622854
1102	64-65	0.18279354599648912
1102	66-67	0.23863913280815297
1102	68-69	0.16440696673389965
1102	70-71	0.1149685580268951
1102	72-73	0.11542705564272637
1102	74-75	0.07969458209382196
1102	76-77	0.06230063375326722
1102	78-79	0.1593879089458241
1102	80-81	0.13175870206763562
1102	82-83	0.09022765625937623
1102	84-85	0.08190367145028432
1102	86-87	0.1130525713191659
1102	88-89	0.10686976592936759
1102	90-91	0.10045474993914283
1102	92-93	0.1724234278447554
1102	94-95	0.14840591410160187
1102	96-97	0.1827120618424587
1102	98-99	0.15742046066174353
1102	100	0.18185342430457396
1103	1	-0.04971537672714277
1103	2	-0.05064577904414236
1103	3	-0.03426964938666899
1103	4	0.1897979285796083
1103	5	0.09197776119368939
1103	6	0.0314960309513026
1103	7	-0.02593195812989535
1103	8	0.07003307334205289
1103	9	0.06336636260824235
1103	10-11	-0.052098554527887586
1103	12-13	0.03366356699976336
1103	14-15	-0.03475295476079765
1103	16-17	0.06222992224095236
1103	18-19	-0.07489126245773292
1103	20-21	-0.1346218765151761
1103	22-23	-0.08425484892190127
1103	24-25	-0.0012695706438705656
1103	26-27	-0.03652521706370493
1103	28-29	-0.06198634538836956
1103	30-31	-0.024351055214410167
1103	32-33	-0.07504408608910751
1103	34-35	-0.09094523566795942
1103	36-37	-0.14137381876608401
1103	38-39	-0.10460080570965147
1103	40-41	-0.05054422800303371
1103	42-43	-0.0915473948654153
1103	44-45	-0.1801804414103998
1103	46-47	-0.22552929498021257
1103	48-49	-0.20680203484386794
1103	50-51	-0.29073221656326353
1103	52-53	-0.36071779442642793
1103	54-55	-0.293380397933106
1103	56-57	-0.2498450438549753
1103	58-59	-0.1884688207424432
1103	60-61	-0.27945274494878447
1103	62-63	-0.2531537151929584
1103	64-65	-0.24432534236975556
1103	66-67	-0.20296981497262578
1103	68-69	-0.2946354399778244
1103	70-71	-0.23254003213233432
1103	72-73	-0.19617531343894257
1103	74-75	-0.2276881151540735
1103	76-77	-0.20935253216658367
1103	78-79	-0.21712801012329308
1103	80-81	-0.22110000870888769
1103	82-83	-0.26007900205443235
1103	84-85	-0.27457482379708864
1103	86-87	-0.27555971690332015
1103	88-89	-0.310414213332848
1103	90-91	-0.3069534933794458
1103	92-93	-0.36232832176812835
1103	94-95	-0.4160497753012997
1103	96-97	-0.447192262263588
1103	98-99	-0.520173057292336
1103	100	-0.5302794358874081
1104	1	-0.1255049844180931
1104	2	-0.10031252373731547
1104	3	-0.08803689221747035
1104	4	-0.007907270860243898
1104	5	-6.392888295181365E-4
1104	6	0.004960996366399684
1104	7	0.010196777388998157
1104	8	-0.19570316148213607
1104	9	-0.2666864353263847
1104	10-11	-0.18178531233498063
1104	12-13	-0.1863824001935086
1104	14-15	-0.17693818968217556
1104	16-17	-0.0953297509238169
1104	18-19	-0.2298125446659114
1104	20-21	-0.2700285930914177
1104	22-23	-0.21825188665251005
1104	24-25	-0.2605484277882937
1104	26-27	-0.20866954967015516
1104	28-29	-0.21317501758259993
1104	30-31	-0.20348574128092878
1104	32-33	-0.23431336103124067
1104	34-35	-0.1633696423585178
1104	36-37	-0.27188519352465335
1104	38-39	-0.22101060091060276
1104	40-41	-0.19573546822746835
1104	42-43	-0.26851670854701837
1104	44-45	-0.2779190416696622
1104	46-47	-0.3554512274092545
1104	48-49	-0.40770433607819
1104	50-51	-0.3986999546570402
1104	52-53	-0.5025332932279625
1104	54-55	-0.4826696859891655
1104	56-57	-0.4802063887033796
1104	58-59	-0.5564530402241559
1104	60-61	-0.5563567627661996
1104	62-63	-0.4263301194304816
1104	64-65	-0.44216749885806905
1104	66-67	-0.4039526751504994
1104	68-69	-0.4610255916640895
1104	70-71	-0.5397513237995142
1104	72-73	-0.5617654881224823
1104	74-75	-0.5381880870919105
1104	76-77	-0.508013693173595
1104	78-79	-0.5252648056432037
1104	80-81	-0.5558831976931913
1104	82-83	-0.6522324103753405
1104	84-85	-0.5951376823596775
1104	86-87	-0.4525841282481018
1104	88-89	-0.5023605776399691
1104	90-91	-0.5661723248709443
1104	92-93	-0.6249366519872446
1104	94-95	-0.6649793748610655
1104	96-97	-0.6443333437656946
1104	98-99	-0.6217097687626421
1104	100	-0.5841147123544772
1105	1	-0.18361251580221705
1105	2	-0.10259912915226721
1105	3	-0.15152689788097362
1105	4	0.02762328655182955
1105	5	-0.036740222636332476
1105	6	-0.017092036559752444
1105	7	-0.09194703261795212
1105	8	-0.09422877205329172
1105	9	-0.12881773731451318
1105	10-11	-0.16304032829209802
1105	12-13	-0.09922298200427804
1105	14-15	-0.0777583238347006
1105	16-17	-0.19346227150433748
1105	18-19	-0.13697741162729926
1105	20-21	-0.18029174462902375
1105	22-23	-0.19222286053329896
1105	24-25	-0.2301415950816974
1105	26-27	-0.18370504721283964
1105	28-29	-0.19801843919677253
1105	30-31	-0.1781753894190885
1105	32-33	-0.21333679611971945
1105	34-35	-0.22010317055926976
1105	36-37	-0.23994398121311633
1105	38-39	-0.2745943093502987
1105	40-41	-0.3855506931168122
1105	42-43	-0.4242407376321893
1105	44-45	-0.46412385108342846
1105	46-47	-0.3982886419534495
1105	48-49	-0.4526960287137527
1105	50-51	-0.4902173169144888
1105	52-53	-0.4274940379113872
1105	54-55	-0.4241844983286214
1105	56-57	-0.4497702990428962
1105	58-59	-0.5025324741960446
1105	60-61	-0.5190368339491727
1105	62-63	-0.3739145418472134
1105	64-65	-0.3898321765886976
1105	66-67	-0.449842609953123
1105	68-69	-0.3911779693955211
1105	70-71	-0.35143706116869566
1105	72-73	-0.36750320624112476
1105	74-75	-0.4132360658121712
1105	76-77	-0.41362445221707844
1105	78-79	-0.36800357862266253
1105	80-81	-0.37295301981627915
1105	82-83	-0.3507017885442849
1105	84-85	-0.28445996783776195
1105	86-87	-0.35661783011169135
1105	88-89	-0.37796792784570243
1105	90-91	-0.3802455907253943
1105	92-93	-0.4904743760109618
1105	94-95	-0.51978489664139
1105	96-97	-0.44028971064252787
1105	98-99	-0.4724417294348555
1105	100	-0.42700674882635425
1106	1	0.03221148075135716
1106	2	-0.03533861949594197
1106	3	0.026311019082164933
1106	4	0.02123592024157972
1106	5	0.06805084433251096
1106	6	0.0224295153297831
1106	7	0.0772886918846325
1106	8	-0.01512055602956508
1106	9	0.06551188624538895
1106	10-11	0.10269101832307115
1106	12-13	0.12297380414665326
1106	14-15	0.030567310623311528
1106	16-17	0.06873169765291465
1106	18-19	-0.05226106875245051
1106	20-21	-0.02969607955025566
1106	22-23	0.14114882448290444
1106	24-25	0.10614060287936411
1106	26-27	0.05144167750075468
1106	28-29	0.052844221209625175
1106	30-31	0.05668096453932492
1106	32-33	0.059607103644481185
1106	34-35	0.1232994443098363
1106	36-37	0.039298134212145897
1106	38-39	0.04587696280246689
1106	40-41	0.053921218918517866
1106	42-43	-0.006980135773765994
1106	44-45	-0.04108320936384047
1106	46-47	-0.016959220272241282
1106	48-49	-0.030401894707928534
1106	50-51	-0.03242216943196752
1106	52-53	-0.09594898625318393
1106	54-55	-0.11860777287829194
1106	56-57	-0.12406146466798873
1106	58-59	-0.1717969569725568
1106	60-61	-0.1286622545773497
1106	62-63	-0.06971632559781682
1106	64-65	-0.0380895305623028
1106	66-67	-0.005325838083855672
1106	68-69	-0.08461490100798841
1106	70-71	-0.07624437414749963
1106	72-73	-0.03871831224675759
1106	74-75	-0.0286057431057003
1106	76-77	0.09266092100867951
1106	78-79	0.01943315646762045
1106	80-81	0.003603208931686197
1106	82-83	0.036193566551027345
1106	84-85	0.04474332611298237
1106	86-87	-0.042686299215247914
1106	88-89	-0.05626527469955178
1106	90-91	-0.10001133680239249
1106	92-93	-0.07441652858927839
1106	94-95	-0.09599928390970547
1106	96-97	-0.07596373916388544
1106	98-99	-0.16853998684151605
1106	100	-0.11935086602944978
1107	1	-0.5365870973756266
1107	2	-0.5797944292873218
1107	3	-0.5055889529650663
1107	4	-0.39293626680359495
1107	5	-0.3979497542896624
1107	6	-0.3906524140419094
1107	7	-0.43653807914307663
1107	8	-0.5221393989186609
1107	9	-0.5132845673325193
1107	10-11	-0.46243673640512384
1107	12-13	-0.4820757765466652
1107	14-15	-0.45996551734760516
1107	16-17	-0.4237415980840993
1107	18-19	-0.4756491436068657
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2216	54-55	0.34380636037020196
2216	56-57	0.3862292471336417
2216	58-59	0.48783998520296734
2216	60-61	0.4240691014133944
2216	62-63	0.3929782261517474
2216	64-65	0.3103525943925405
2216	66-67	0.3184237844194655
2216	68-69	0.30152681844200657
2216	70-71	0.17338140944622893
2216	72-73	0.23936816722316223
2216	74-75	0.2281826187481073
2216	76-77	0.33289083190881996
2216	78-79	0.2951338002226862
2216	80-81	0.23502049634307554
2216	82-83	0.26271355717194567
2216	84-85	0.2734592515858836
2216	86-87	0.22571060841143975
2216	88-89	0.2287940417481238
2216	90-91	0.2590255363775924
2216	92-93	0.28917812488047545
2216	94-95	0.22912106465395254
2216	96-97	0.2541898510936704
2216	98-99	0.20698061812908364
2216	100	0.26780507651644214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86593.0
3	18563.0
4	13660.0
5	12194.0
6	7893.0
7	6140.0
8	6837.0
9	6918.0
10	6914.0
11	7659.0
12	8944.0
13	10138.0
14	10738.0
15	10589.0
16	10251.0
17	10152.0
18	9680.0
19	9348.0
20	9027.0
21	9938.0
22	12103.0
23	19297.0
24	31447.0
25	41979.0
26	45970.0
27	34207.0
28	29695.0
29	36231.0
30	43212.0
31	47518.0
32	60363.0
33	80703.0
34	115177.0
35	183742.0
36	304848.0
37	550329.0
38	853775.0
39	466100.0
40	2248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.534593222887594	22.524016289376718	14.614057415867416	21.32733307186827
2	14.63738264213462	23.44227623725665	40.04353913251595	21.876801988092772
3	16.97957416963575	30.388278983412093	30.539185242626694	22.092961604325463
4	11.445824033790553	20.554379344458578	39.08709828532914	28.912698336421727
5	11.551044492519361	41.82447514913681	34.155840249744266	12.468640108599564
6	26.101619742542177	41.57247307991426	19.02126630249792	13.304640875045646
7	23.814654938784184	37.16443885836193	22.997349673657084	16.023556529196796
8	23.52817845970699	38.14328173132996	21.042059653596507	17.286480155366554
9	24.836299123037097	19.890976532052857	21.504589508341507	33.768134836568535
10-11	21.739065125783654	30.783266714114667	28.738759748983988	18.7389084111177
12-13	22.503238806335332	29.350817157884336	28.178838477174118	19.967105558606214
14-15	20.685238155999023	30.537487782186584	26.032722680102026	22.744551381712363
16-17	19.543898284272547	33.31443709946475	26.957730606185095	20.18393401007761
18-19	19.71976110661008	32.15891972716304	28.726236400890958	19.395082765335932
20-21	21.799396905738995	30.909873907060053	27.873172123126867	19.41755706407408
22-23	21.365294819578263	30.954406553053403	27.981186822661662	19.69911180470667
24-25	20.720474911254552	31.02253462326775	28.066278340164352	20.190712125313343
26-27	20.29354044094248	31.184235201531475	28.139207631593088	20.38301672593296
28-29	20.301417163007425	31.08106664213804	28.387640855708344	20.229875339146183
30-31	20.53592238181806	31.400281198813197	27.958963990353325	20.104832429015417
32-33	20.283779780547395	31.233150701350482	28.294015141508172	20.18905437659395
34-35	20.725008842288876	30.91741147335761	28.163774056135992	20.193805628217525
36-37	20.673076848618884	30.96661486174125	27.941532450094204	20.418775839545667
38-39	20.46293036949532	31.05170915068157	28.17176698368166	20.313593496141447
40-41	20.478455532829283	30.74351361697541	28.355029974318345	20.423000875876962
42-43	20.347549057792126	30.944416216416283	28.333136915813384	20.374897809978208
44-45	20.502518197652574	30.937140000266396	28.16698319022916	20.39335861185187
46-47	20.558434120978625	31.035063229154698	28.076058343835648	20.33044430603103
48-49	20.53071189447835	30.797895340682977	28.082650034567607	20.588742730271065
50-51	20.42450366191477	30.77772345377002	28.082583298088114	20.715189586227094
52-53	20.476392245628805	30.828810445710015	28.137781358300355	20.557015950360825
54-55	20.556206691057405	31.094008823780843	27.969663142688294	20.38012134247346
56-57	20.569564876703556	30.850406177226386	27.811781168205908	20.768247777864154
58-59	20.605718857504634	30.611295760299424	28.052053364191888	20.730932018004058
60-61	20.601325024313795	30.893090546425405	27.833935241682205	20.671649187578595
62-63	20.453664913439642	30.83370729935213	28.05006839784286	20.662559389365367
64-65	20.570094770605703	30.707447806016887	28.010761904629174	20.71169551874824
66-67	20.381000725814484	30.71431015955995	27.94898330998349	20.955705804642086
68-69	20.627335489624546	30.968815024188977	27.76511673750982	20.638732748676656
70-71	20.551272303835415	30.626543635229027	27.876360528153583	20.945823532781972
72-73	20.446437945042025	30.74222472313056	28.051773138159252	20.75956419366817
74-75	20.46050938390111	30.860118412539823	27.917938133985114	20.761434069573948
76-77	20.495739503929098	30.723779489905017	27.81313221413236	20.96734879203353
78-79	20.52061803706011	30.662587177327556	28.09196999971042	20.724824785901912
80-81	20.45210313210694	30.751189959045433	27.945047005995676	20.851659902851953
82-83	20.38433030793071	30.782756722752712	28.175596420809214	20.657316548507364
84-85	20.288623289026884	30.636961634493304	28.040367985246395	21.03404709123342
86-87	20.58201929213443	30.628597994531106	27.891609468623063	20.897773244711402
88-89	20.521380374131382	30.561382913975184	28.014926228378418	20.90231048351502
90-91	20.348928328067895	30.554698630985566	27.928993991203615	21.167379049742923
92-93	20.4469534656268	30.578587263331396	27.917914002431804	21.05654526861
94-95	20.375996376460932	30.458184126885808	28.109000391001544	21.056819105651712
96-97	20.381896736343958	30.664764682937072	27.87625357725263	21.07708500346634
98-99	20.279401236163395	30.630407401803588	27.8720294763844	21.218161885648616
100	20.316886433282153	30.653996826241524	27.828094174452637	21.201022566023685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26550.0
1	19328.0
2	10647.0
3	7654.0
4	5209.5
5	3709.0
6	2980.5
7	2918.0
8	3168.5
9	3221.5
10	2620.0
11	2004.5
12	1877.5
13	1899.0
14	1970.0
15	2137.5
16	2407.0
17	2817.0
18	3508.0
19	4455.5
20	5677.0
21	7747.5
22	11612.0
23	17765.5
24	24158.0
25	30134.5
26	37980.0
27	47350.5
28	59795.5
29	71010.0
30	76097.0
31	81937.5
32	89199.5
33	95988.0
34	102188.0
35	106238.0
36	107792.5
37	107747.5
38	107199.5
39	105780.0
40	105970.5
41	104177.5
42	99974.0
43	98964.5
44	98478.0
45	96378.0
46	95125.0
47	92986.5
48	89978.5
49	88831.0
50	88989.0
51	85425.5
52	78059.0
53	72418.5
54	69605.0
55	68586.0
56	62544.0
57	53880.5
58	48507.5
59	44454.0
60	38905.0
61	32121.5
62	26658.0
63	21634.5
64	17272.5
65	13872.0
66	10915.5
67	8238.0
68	6213.0
69	4851.5
70	3631.5
71	2766.0
72	2117.5
73	1574.0
74	1308.5
75	1042.0
76	734.5
77	576.0
78	453.5
79	314.5
80	225.0
81	163.5
82	132.5
83	114.5
84	94.5
85	77.5
86	71.5
87	71.5
88	61.0
89	47.5
90	43.0
91	38.0
92	35.0
93	31.0
94	26.5
95	25.5
96	22.5
97	18.0
98	19.0
99	25.5
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17656416350986656
2	0.1707147985837728
3	0.1838062343707445
4	0.1734073633910223
5	0.1875820149050484
6	0.07418480279283964
7	0.07616554012231053
8	0.06533338285176657
9	0.05901978261407809
10-11	0.07186362623486593
12-13	0.07139939092327119
14-15	0.06895441828220555
16-17	0.06442038673896358
18-19	0.06907821436529749
20-21	0.0703007006858303
22-23	0.06849018297061081
24-25	0.06876872415756766
26-27	0.07215764193220926
28-29	0.06931033202109485
30-31	0.07562393225878333
32-33	0.07160055955829557
34-35	0.071739830151774
36-37	0.08240176780806656
38-39	0.07779036371289212
40-41	0.07291589294114734
42-43	0.07593342246651316
44-45	0.08724528955903836
46-47	0.07635123424694842
48-49	0.08244819133922603
50-51	0.0789045284607195
52-53	0.07864146178414916
54-55	0.07960088142811161
56-57	0.07294684196192032
58-59	0.08125665403946619
60-61	0.07737255193245686
62-63	0.06861397905370274
64-65	0.0719100497660254
66-67	0.07221953997375523
68-69	0.07036259872737627
70-71	0.06607615935031816
72-73	0.06409542202084725
74-75	0.06929485751070835
76-77	0.07390626160588279
78-79	0.07070303795587908
80-81	0.07868788531530863
82-83	0.07950803436579267
84-85	0.08111738344598778
86-87	0.07732612840129739
88-89	0.07969372849043056
90-91	0.07799153234791652
92-93	0.06926390848993538
94-95	0.06909368887568398
96-97	0.06841281041867836
98-99	0.07741897546361634
100	0.08575973656193518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3231120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.43300443218801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69679169587037	43.647579602350184
2	15.220983033857957	17.788155381593608
3	4.888683422402342	8.569813802664001
4	1.9841125141141378	4.637506213247644
5	0.9746372100953778	2.8475490208639282
6	0.5643982593064257	1.9787691592542942
7	0.35887089311328074	1.4678933141510122
8	0.2328224416008331	1.088361180957945
9	0.16950096638587583	0.8914005648077431
>10	0.8105489675776928	8.521373791819203
>50	0.06082577727354743	2.4624126136130906
>100	0.03506586438469321	3.801421211711662
>500	0.002122272322421096	0.8586194160446333
>1k	5.836248870930345E-4	0.5227363041924935
>5k	0.0	0.0
>10k+	5.3056807917548585E-5	0.9164084227285402
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29559	0.914822105028597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.5474510386491372E-5	0.0
14-15	0.0	0.0	0.0	7.737255193245685E-5	0.0
16-17	0.0	0.0	0.0	9.284706231894823E-5	0.0
18-19	0.0	0.0	0.0	1.3927059347842234E-4	0.0
20-21	0.0	0.0	0.0	2.4759216618386196E-4	0.0
22-23	0.0	0.0	0.0	5.261333531407066E-4	0.0
24-25	0.0	0.0	0.0	9.284706231894823E-4	0.0
26-27	0.0	1.5474510386491372E-5	0.0	0.0015938745698086112	0.0
28-29	0.0	3.0949020772982745E-5	0.0	0.003311545222709153	0.0
30-31	0.0	3.0949020772982745E-5	0.0	0.0078920002971106	0.0
32-33	0.0	3.0949020772982745E-5	0.0	0.015041224095669613	0.0
34-35	0.0	3.0949020772982745E-5	0.0	0.02629119314664884	0.0
36-37	0.0	3.0949020772982745E-5	0.0	0.04410235460150041	0.0
38-39	0.0	3.0949020772982745E-5	0.0	0.06917106142761642	0.0
40-41	0.0	3.0949020772982745E-5	0.0	0.09659189383247914	0.0
42-43	0.0	3.0949020772982745E-5	0.0	0.12421389487236623	0.0
44-45	0.0	3.0949020772982745E-5	0.0	0.15706628042288742	0.0
46-47	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.19897125454950604	0.0
48-49	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.2471898289138132	0.0
50-51	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.29542387778850676	0.0
52-53	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.34246638936344054	0.0
54-55	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.39641053257074943	0.0
56-57	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.46418888806358166	0.0
58-59	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.5396116516873406	0.0
60-61	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.6232978038574859	0.0
62-63	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.7085468815766669	0.0
64-65	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.7989180222337766	0.0
66-67	3.0949020772982745E-5	3.0949020772982745E-5	0.0	0.9071622223872837	0.0
68-69	3.0949020772982745E-5	3.0949020772982745E-5	0.0	1.0360339448859839	0.0
70-71	3.0949020772982745E-5	3.0949020772982745E-5	0.0	1.1783220678897721	0.0
72-73	3.0949020772982745E-5	3.0949020772982745E-5	0.0	1.3255310851964643	0.0
74-75	3.0949020772982745E-5	3.0949020772982745E-5	0.0	1.4770265418802149	0.0
76-77	3.0949020772982745E-5	3.0949020772982745E-5	0.0	1.6440274579712297	0.0
78-79	3.0949020772982745E-5	3.0949020772982745E-5	0.0	1.843571269405036	0.0
80-81	3.0949020772982745E-5	3.0949020772982745E-5	0.0	2.064655599296838	0.0
82-83	3.0949020772982745E-5	3.0949020772982745E-5	0.0	2.303922478892768	0.0
84-85	3.0949020772982745E-5	3.0949020772982745E-5	0.0	2.543235782019857	0.0
86-87	3.0949020772982745E-5	3.0949020772982745E-5	0.0	2.8005768897472083	0.0
88	3.0949020772982745E-5	3.0949020772982745E-5	0.0	3.0118349055435885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5245	0.0	50.502354	1
GTATCAA	9505	0.0	39.648712	1
ATCAACG	11285	0.0	32.64239	3
TCAACGC	11295	0.0	32.568306	4
TATCAAC	12160	0.0	30.75828	2
CAACGCA	12340	0.0	29.84795	5
AACGCAG	13155	0.0	28.008028	6
GTACATG	20445	0.0	26.188093	1
TACATGG	20290	0.0	25.621746	2
CATGGGT	10305	0.0	25.426222	4
CGCAGAG	14415	0.0	25.136053	8
ACGCAGA	14845	0.0	24.66122	7
TACCTGG	2505	0.0	24.603228	2
ACATGGG	20870	0.0	24.052694	3
GTGGTAT	2540	0.0	23.70964	1
ATGGGTG	5640	0.0	22.852797	5
GCAGAGT	15535	0.0	22.597828	9
TGGTATC	2650	0.0	22.191805	2
GTACCTG	3040	0.0	21.20292	1
ATGGGTA	4190	0.0	20.657211	5
>>END_MODULE