FastQCFastQC Report
Wed 25 May 2016
SRR2431419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231120
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373711.1565958553071383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313460.970128005149917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197350.6107789249548145No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62920.19473123870360742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.1922553170417688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61850.19141969348089827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.15777810790066601No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43410.1343496991755181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119050.067.381751
GTATCAA240600.046.25891
GTGGTAT35350.041.899981
TGGTATC35750.041.297722
TATCAAC313850.035.4608082
ATCAACG314950.035.2462923
TCAACGC318450.034.8731354
CAACGCA328300.033.812515
AACGCAG339000.032.773696
CGCAGAG380100.028.9702328
ACGCAGA381900.028.9690577
GCAGAGT401350.027.1553259
CATGGGT100100.027.1015454
GTACATG201800.026.0037331
TACATGG200750.025.6935182
TACCTGG22400.025.608512
ACATGGG201000.024.8420543
GAGTACT286200.023.77787812-13
CAGAGTA395500.023.4157210-11
ATGGGTG52500.023.284475
Produced by FastQC (version 0.11.5)