##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431419_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3231120
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62742330832653	34.0	31.0	34.0	31.0	34.0
2	32.71909987867984	34.0	31.0	34.0	31.0	34.0
3	32.812396011290204	34.0	31.0	34.0	31.0	34.0
4	36.292207036569366	37.0	37.0	37.0	35.0	37.0
5	36.209601314714405	37.0	37.0	37.0	35.0	37.0
6	36.26073095397262	37.0	37.0	37.0	35.0	37.0
7	36.214156701081976	37.0	37.0	37.0	35.0	37.0
8	36.22842853252123	37.0	37.0	37.0	35.0	37.0
9	38.00953817871203	39.0	39.0	39.0	35.0	39.0
10-11	37.9902665329669	39.0	39.0	39.0	35.0	39.0
12-13	37.97196668647404	39.0	39.0	39.0	35.0	39.0
14-15	39.39064178984377	41.0	39.0	41.0	36.0	41.0
16-17	39.421034347223255	41.0	39.0	41.0	36.0	41.0
18-19	39.526460484290276	41.0	39.5	41.0	36.5	41.0
20-21	39.534968679590975	41.0	40.0	41.0	36.0	41.0
22-23	39.46620134813935	41.0	39.5	41.0	36.0	41.0
24-25	39.41068468518656	41.0	39.0	41.0	36.0	41.0
26-27	39.28190534551487	41.0	39.0	41.0	35.5	41.0
28-29	39.19150031568002	41.0	39.0	41.0	35.0	41.0
30-31	38.96468546510188	41.0	38.5	41.0	35.0	41.0
32-33	38.682899582807195	41.0	38.0	41.0	35.0	41.0
34-35	38.52899938720939	41.0	38.0	41.0	35.0	41.0
36-37	38.36759792270173	40.0	38.0	41.0	35.0	41.0
38-39	38.20931921439006	40.0	38.0	41.0	34.5	41.0
40-41	38.045778553566564	40.0	38.0	41.0	34.0	41.0
42-43	37.83555175914234	40.0	37.5	41.0	33.5	41.0
44-45	37.5348054235064	40.0	37.0	41.0	33.0	41.0
46-47	37.07526925648072	40.0	37.0	41.0	31.5	41.0
48-49	36.65720911015376	40.0	37.0	41.0	30.5	41.0
50-51	36.457455000123794	40.0	36.0	41.0	30.0	41.0
52-53	36.384856334645576	40.0	36.0	41.0	30.0	41.0
54-55	36.239174342023816	40.0	36.0	41.0	30.0	41.0
56-57	36.08202774889203	40.0	35.0	41.0	30.0	41.0
58-59	35.897666443833714	40.0	35.0	41.0	29.0	41.0
60-61	35.7388010968333	39.0	35.0	41.0	29.0	41.0
62-63	35.576846728069526	39.0	35.0	41.0	29.0	41.0
64-65	35.340067066528015	39.0	35.0	41.0	28.0	41.0
66-67	35.0802529772958	38.0	35.0	41.0	28.0	41.0
68-69	34.79121759637525	37.0	35.0	40.5	28.0	41.0
70-71	34.4951674651514	37.0	35.0	40.0	27.5	41.0
72-73	34.18202960583327	36.5	35.0	39.5	26.5	41.0
74-75	33.85547627448068	36.0	35.0	39.0	26.0	41.0
76-77	33.53038884349699	35.5	34.0	39.0	26.0	41.0
78-79	33.21668600980465	35.0	34.0	37.5	26.0	39.5
80-81	32.959307299017055	35.0	34.0	37.0	26.0	39.0
82-83	32.758458986357674	35.0	34.0	37.0	26.0	39.0
84-85	32.50058957884572	35.0	34.0	36.0	26.0	38.0
86-87	32.26450023521256	35.0	34.0	36.0	26.0	37.0
88-89	32.031398864789914	35.0	34.0	36.0	25.0	37.0
90-91	31.84786885042957	35.0	34.0	35.0	25.0	36.5
92-93	31.686738654088984	35.0	34.0	35.0	24.5	36.0
94-95	31.547819641486544	35.0	34.0	35.0	24.0	36.0
96-97	31.404905574537622	35.0	34.0	35.0	23.5	36.0
98-99	31.290104050607837	35.0	34.0	35.0	21.0	36.0
100	31.185630369655104	35.0	34.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.023918581930871596
1101	2	-0.0021562994811930025
1101	3	0.0025640381770557497
1101	4	0.026709522838579858
1101	5	0.012331236380937582
1101	6	-0.023444996927729278
1101	7	0.012784905339287889
1101	8	0.037471910016790844
1101	9	0.029809961428306053
1101	10-11	-0.009830285279193163
1101	12-13	-0.006900501152273364
1101	14-15	-6.156865342674678E-4
1101	16-17	-0.044034784266706595
1101	18-19	-0.04418250418826375
1101	20-21	-0.048995516823268304
1101	22-23	-0.05779172066571192
1101	24-25	0.014323907518317469
1101	26-27	0.03740945075868041
1101	28-29	-0.030692314905273577
1101	30-31	-0.061194485503868634
1101	32-33	-0.0801069431493886
1101	34-35	-0.0824680231242354
1101	36-37	-0.021567321935918926
1101	38-39	0.029292688376237663
1101	40-41	-0.011303646071915807
1101	42-43	-0.00961720068954719
1101	44-45	-0.09239528010066067
1101	46-47	-0.17941843457339957
1101	48-49	-0.012714536953957634
1101	50-51	-0.12737894582857479
1101	52-53	-0.13851666295987997
1101	54-55	-0.09568649439192001
1101	56-57	-0.09512595739656149
1101	58-59	-0.0869702755976931
1101	60-61	-0.0957528284338025
1101	62-63	-0.08422970056569312
1101	64-65	-0.11105721291179549
1101	66-67	-0.06243356811429379
1101	68-69	-0.08165654382305831
1101	70-71	-0.09189293401804832
1101	72-73	-0.05870080864267635
1101	74-75	-0.06540777368618222
1101	76-77	-0.14304334872014834
1101	78-79	-0.10820289486011347
1101	80-81	-0.0167777487741958
1101	82-83	-0.05167874685293583
1101	84-85	0.012072792330307891
1101	86-87	-0.019580371786716455
1101	88-89	-0.004186179135139412
1101	90-91	-0.02263762587557494
1101	92-93	-0.008464849485214643
1101	94-95	-0.06607294600650704
1101	96-97	-0.10450729387700619
1101	98-99	-0.019640961659710854
1101	100	0.015056913283746809
1102	1	0.028290437663343937
1102	2	0.0423032922929778
1102	3	0.028639616265323298
1102	4	0.0467844259609933
1102	5	0.04642567543337606
1102	6	0.014130514282747697
1102	7	-0.013534193576532516
1102	8	0.00828977538252218
1102	9	0.031093729356193478
1102	10-11	0.020824255484598098
1102	12-13	0.02278314549119642
1102	14-15	0.04711146827519741
1102	16-17	0.05135626515288294
1102	18-19	0.09050629037888314
1102	20-21	0.08459626782359209
1102	22-23	0.11336724521805053
1102	24-25	0.11360588759588097
1102	26-27	0.15704243688284691
1102	28-29	0.0839214337399099
1102	30-31	0.08194194946736388
1102	32-33	0.05232292918304893
1102	34-35	0.05878890800877912
1102	36-37	0.02117074989891421
1102	38-39	0.0826811312280995
1102	40-41	0.04046842490706837
1102	42-43	0.039680296938790605
1102	44-45	0.08463788795013727
1102	46-47	0.005169043715120836
1102	48-49	-0.005391014584169795
1102	50-51	-0.052453546691936026
1102	52-53	-0.07236936617911027
1102	54-55	-0.0894522707668699
1102	56-57	-0.024668779886724224
1102	58-59	-0.045844339249164534
1102	60-61	-0.09540958592256743
1102	62-63	0.020576051590460054
1102	64-65	-0.07281311422207182
1102	66-67	-0.0519708595019992
1102	68-69	-0.09596258324430096
1102	70-71	-0.11210763789701161
1102	72-73	-0.050707484997495555
1102	74-75	-0.023501628212471815
1102	76-77	-0.018427205802559854
1102	78-79	0.021940685324452147
1102	80-81	-0.011389612465364962
1102	82-83	-0.06794770884778956
1102	84-85	-0.05106774098792499
1102	86-87	-0.043507625889468216
1102	88-89	-0.015213993154915784
1102	90-91	0.03735089304084127
1102	92-93	0.034722184125516975
1102	94-95	0.037763197225249456
1102	96-97	0.03837602889709757
1102	98-99	0.0857489358435366
1102	100	0.10805902148105773
1103	1	-0.09111888093389098
1103	2	-0.125291681084029
1103	3	-0.07713269418459134
1103	4	-0.01813010837047102
1103	5	-0.010454549353298148
1103	6	-0.006886214643671451
1103	7	-0.10196456565153511
1103	8	0.003529206516056149
1103	9	-0.006445401247745508
1103	10-11	-0.03557525787446991
1103	12-13	-0.01477600010757385
1103	14-15	-0.05632459541747892
1103	16-17	-0.06258014763037067
1103	18-19	-0.09562481814950274
1103	20-21	-0.15550016507777542
1103	22-23	-0.08137980298035075
1103	24-25	-0.1244210323378212
1103	26-27	-0.07182300586922707
1103	28-29	-0.07952914668322109
1103	30-31	-0.10275051685576386
1103	32-33	-0.1096177612780167
1103	34-35	-0.0852923603700404
1103	36-37	-0.09576760743249224
1103	38-39	-0.18065739803539316
1103	40-41	-0.1666785922610643
1103	42-43	-0.13254428067802593
1103	44-45	-0.20508298255683144
1103	46-47	-0.2057681197476242
1103	48-49	-0.1420889294857588
1103	50-51	-0.24663248777683577
1103	52-53	-0.14225903293412756
1103	54-55	-0.08856148631266336
1103	56-57	-0.0991894805240392
1103	58-59	-0.06154354809438445
1103	60-61	-0.15702244295526668
1103	62-63	-0.14670261924513994
1103	64-65	-0.139942328825704
1103	66-67	-0.12393669526513662
1103	68-69	-0.08983166689736777
1103	70-71	-0.05353729444766486
1103	72-73	-0.15225288165414952
1103	74-75	-0.06946128276398156
1103	76-77	-0.026637009822003677
1103	78-79	-0.05649500923922801
1103	80-81	-0.031963152220328084
1103	82-83	-0.12615518090940725
1103	84-85	-0.08871906337207491
1103	86-87	-0.026289925790493385
1103	88-89	0.005919169605661523
1103	90-91	-0.005561724632212162
1103	92-93	-0.04608280302996448
1103	94-95	-0.045204768705197296
1103	96-97	-0.013775688513675277
1103	98-99	-0.06233024572598467
1103	100	-0.06077650658786382
1104	1	-0.10228419993224946
1104	2	-0.08344113879982729
1104	3	-0.04908243964023029
1104	4	-0.026966404657635223
1104	5	-0.021402352359643828
1104	6	-0.03948774149916545
1104	7	-0.05339717333838223
1104	8	-0.1222174865974921
1104	9	-0.03173160109689377
1104	10-11	-0.07794515552286896
1104	12-13	-0.06491617403823824
1104	14-15	-0.08988584827674373
1104	16-17	0.011315469333410988
1104	18-19	-0.03260007279652655
1104	20-21	-0.017513098313095554
1104	22-23	-0.022705103690398687
1104	24-25	-0.027848175431579136
1104	26-27	-0.11215127843086492
1104	28-29	-0.09473925015731766
1104	30-31	-0.1221349698694354
1104	32-33	-0.059551849415115043
1104	34-35	-0.05498269096970887
1104	36-37	-0.05025666387302863
1104	38-39	-0.11082696194937114
1104	40-41	-0.18207554867984044
1104	42-43	-0.14102446126146617
1104	44-45	-0.08806225351550268
1104	46-47	-0.10771289614692847
1104	48-49	-0.056661542730275016
1104	50-51	-0.16023681947983448
1104	52-53	-0.09944903842336572
1104	54-55	-0.04627098405468644
1104	56-57	-0.06710696681123096
1104	58-59	-0.048011356685783824
1104	60-61	-0.14239527960198473
1104	62-63	-0.09835568602898803
1104	64-65	-0.10134761354715494
1104	66-67	-0.1518635447328549
1104	68-69	-0.16080929793056242
1104	70-71	-0.156443918624106
1104	72-73	-0.1121953747515434
1104	74-75	-0.14252181079807968
1104	76-77	-0.1993970673699792
1104	78-79	-0.236216742084693
1104	80-81	-0.21216420051325713
1104	82-83	-0.14675506783942183
1104	84-85	-0.12794250800953222
1104	86-87	-0.1380819208186992
1104	88-89	-0.12672711982338924
1104	90-91	-0.1396537630541026
1104	92-93	-0.15820962758394685
1104	94-95	-0.18108669407079248
1104	96-97	-0.19411348852216292
1104	98-99	-0.13781342827651955
1104	100	-0.08005439182319307
1105	1	-0.14988580074392388
1105	2	-0.1767633061764471
1105	3	-0.10880903474814829
1105	4	-0.05847641241475543
1105	5	-0.08691216149157555
1105	6	-0.019896050474073945
1105	7	-0.015195375511986242
1105	8	-0.04696511181859364
1105	9	-0.027760170914987725
1105	10-11	-0.02978565949402423
1105	12-13	-0.061485326472329405
1105	14-15	-0.11861282184617039
1105	16-17	-0.09942559051987132
1105	18-19	-0.12253675430639532
1105	20-21	-0.08786989894440467
1105	22-23	-0.1673736277710134
1105	24-25	-0.1777612098071586
1105	26-27	-0.11118392006539324
1105	28-29	-0.11141557967113869
1105	30-31	-0.13413233405947267
1105	32-33	-0.1861739327786438
1105	34-35	-0.2005859613066434
1105	36-37	-0.12390151666223659
1105	38-39	-0.147178324545024
1105	40-41	-0.14053590819143125
1105	42-43	-0.1962122023293702
1105	44-45	-0.17777803160285544
1105	46-47	-0.27713521893434034
1105	48-49	-0.33225735120191047
1105	50-51	-0.22227256664606898
1105	52-53	-0.3036172149098988
1105	54-55	-0.21594480928062154
1105	56-57	-0.15854286589889455
1105	58-59	-0.25253169026874644
1105	60-61	-0.20422327555432673
1105	62-63	-0.23332807981147852
1105	64-65	-0.2219037104011008
1105	66-67	-0.2582947063412817
1105	68-69	-0.18025067799987227
1105	70-71	-0.24807669822261857
1105	72-73	-0.16640555778197097
1105	74-75	-0.13526259687069597
1105	76-77	-0.19267373884122918
1105	78-79	-0.1291564217613086
1105	80-81	-0.10702531050598196
1105	82-83	-0.16639171794504648
1105	84-85	-0.20389101040242252
1105	86-87	-0.16499120547275936
1105	88-89	-0.10931010623366788
1105	90-91	-0.14075344874315832
1105	92-93	-0.15693668167710584
1105	94-95	-0.15407339960357902
1105	96-97	-0.17090104814327134
1105	98-99	-0.1953763508583215
1105	100	-0.15266290425471496
1106	1	-0.1316168767239887
1106	2	-0.08815695671836465
1106	3	-0.08240046546744395
1106	4	-0.06855862419735814
1106	5	-0.04121522030940383
1106	6	-0.09946312561360315
1106	7	-0.07029183778767134
1106	8	-0.04793608773918123
1106	9	-0.0644264433079158
1106	10-11	-0.08574317961581812
1106	12-13	-0.14199143609079812
1106	14-15	-0.16496283928115218
1106	16-17	-0.09412524305844983
1106	18-19	-0.04845820672304768
1106	20-21	-0.031215550478250975
1106	22-23	-0.08892617150840465
1106	24-25	-0.02088414497406177
1106	26-27	-0.0431461247563405
1106	28-29	-0.03681330220903334
1106	30-31	-0.08613855076407617
1106	32-33	-0.07392931393466284
1106	34-35	-0.0862620533988121
1106	36-37	-0.02816377385569524
1106	38-39	0.012279718538295015
1106	40-41	-0.04517437190424545
1106	42-43	-0.052487327950345275
1106	44-45	-0.08838295904111249
1106	46-47	-0.13813284828663797
1106	48-49	-0.10585880560660854
1106	50-51	-0.22092959915924837
1106	52-53	-0.20429965972672193
1106	54-55	-0.17770291276948313
1106	56-57	-0.14652441002414207
1106	58-59	-0.19872656230408126
1106	60-61	-0.12832854418427075
1106	62-63	-0.22572035285403302
1106	64-65	-0.231514615784441
1106	66-67	-0.1390245509403556
1106	68-69	-0.2171863341938689
1106	70-71	-0.17496708494193314
1106	72-73	-0.10899480085278412
1106	74-75	-0.09410750916322286
1106	76-77	-0.17067352756669862
1106	78-79	-0.15000296524070222
1106	80-81	-0.11288115366476603
1106	82-83	-0.14355360911372372
1106	84-85	-0.07824865813191906
1106	86-87	-0.11712165353017667
1106	88-89	-0.14911695776290657
1106	90-91	-0.2131789224446159
1106	92-93	-0.1702191624790892
1106	94-95	-0.19299164242123368
1106	96-97	-0.24383857279087096
1106	98-99	-0.21602551598587283
1106	100	-0.20422609426120175
1107	1	-0.2731918465596834
1107	2	-0.20735878050730605
1107	3	-0.1962705691399762
1107	4	-0.21615270143870902
1107	5	-0.23144809075828476
1107	6	-0.2824010745937926
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2216	20-21	0.020593908634445768
2216	22-23	-0.06088719385095942
2216	24-25	-0.00853773416775283
2216	26-27	0.020919227524480277
2216	28-29	0.0019106221506390852
2216	30-31	-0.05617194636456446
2216	32-33	0.05386221101855426
2216	34-35	0.08419636551107601
2216	36-37	0.05984025778749924
2216	38-39	0.07184417213233729
2216	40-41	0.10332531907687326
2216	42-43	0.13736830271130884
2216	44-45	0.13658791218786348
2216	46-47	0.11821390097230022
2216	48-49	0.17825352435321662
2216	50-51	0.33209710498024236
2216	52-53	0.21942242981116067
2216	54-55	0.2523442762650987
2216	56-57	0.2602875662058821
2216	58-59	0.26770802576742625
2216	60-61	0.1844805722203091
2216	62-63	0.1837802543322553
2216	64-65	0.2099579382747052
2216	66-67	0.22402238659527285
2216	68-69	0.23022859199267742
2216	70-71	0.236242080343942
2216	72-73	0.22036143628590565
2216	74-75	0.2131684705869219
2216	76-77	0.23679723266514685
2216	78-79	0.26980707786591296
2216	80-81	0.22875542792962023
2216	82-83	0.24931462585864494
2216	84-85	0.2889248021036579
2216	86-87	0.29437133464199405
2216	88-89	0.2921089024862269
2216	90-91	0.22925044117223692
2216	92-93	0.23008964008372956
2216	94-95	0.2419199875402498
2216	96-97	0.2604419451466242
2216	98-99	0.24931796662360384
2216	100	0.26940102002159705
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71.0
3	394.0
4	98.0
5	157.0
6	369.0
7	812.0
8	1418.0
9	2323.0
10	3310.0
11	4723.0
12	7373.0
13	10724.0
14	12157.0
15	16539.0
16	22133.0
17	34106.0
18	41017.0
19	20870.0
20	12050.0
21	10911.0
22	10240.0
23	10144.0
24	10594.0
25	12065.0
26	14661.0
27	18507.0
28	23069.0
29	28140.0
30	35491.0
31	44525.0
32	55532.0
33	72503.0
34	102140.0
35	177159.0
36	276340.0
37	555137.0
38	958203.0
39	618792.0
40	6323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28561612778924	21.064301620733254	15.326011430092434	21.324070821385074
2	14.64419464063535	22.124123331001737	40.889730050944294	22.341951977418617
3	16.349425549180676	29.43966827252509	32.566697389051235	21.644208789242995
4	10.856173710663795	19.29745722845329	39.913373690856425	29.932995370026493
5	10.798976509506051	39.405498660569975	35.44718261453272	14.348342215391247
6	25.837325757013048	40.78684790413231	18.721929238158904	14.653897100695733
7	23.017529525365816	36.60814206838496	23.596864245215283	16.777464161033944
8	24.897837282428384	34.933119166109584	21.009216618386194	19.159826933075838
9	26.23295520839684	15.715484326018322	22.55203228355254	35.49952818203229
10-11	22.653646413627474	27.877253088712273	29.24810901483077	20.220991482829483
12-13	24.629280965931468	26.744863506286286	29.53029128139643	19.095564246385813
14-15	21.912188639262645	27.187156030595588	29.274234518407628	21.626420811734135
16-17	18.586295154621308	30.432760157468618	30.000943945133574	20.980000742776497
18-19	18.501215136868282	27.37379559858161	34.96267158343824	19.162317681111876
20-21	20.335162420461018	26.246378964569562	34.9869395132338	18.43151910173562
22-23	19.87110347839247	26.310714647154747	35.235316309922354	18.58286556453043
24-25	19.32735047126103	26.489144005263814	35.183224506192595	19.000281017282564
26-27	18.942578645452112	26.71669067798577	35.13613721579084	19.20459346077128
28-29	18.970990686658716	26.592579811783384	35.417901082210726	19.018528419347177
30-31	19.262157586711687	26.821030543683133	34.981275784482726	18.93553608512245
32-33	19.01113559552843	26.79638077670624	35.22336184805081	18.969121779714524
34-35	19.435398223789083	26.61566532547902	34.8627391084406	19.086197342291296
36-37	19.44081261955169	26.64954443272322	34.523997615040045	19.385645332685048
38-39	19.35952003850091	26.822119762897533	34.54836096459634	19.269999234005216
40-41	19.356187989653105	26.71512054356485	34.44527181646949	19.483419650312555
42-43	19.34775732583784	26.965508319462923	34.17982060587453	19.506913748824704
44-45	19.669869072938358	27.034996001376616	33.66102753479298	19.63410739089205
46-47	19.836316388338812	27.37485227606661	32.9400269845222	19.84880435107238
48-49	20.063900561849564	27.479469928947108	32.221317477501564	20.23531203170176
50-51	20.094342308794573	27.65263658945265	31.88994728120959	20.363073820543182
52-53	20.074643402733933	27.937702872338054	31.748298410198895	20.23935531472912
54-55	20.129724225763173	28.20578845805666	31.588816140708154	20.075671175472014
56-57	20.086195090284328	27.899261616026404	31.557941438405717	20.456601855283548
58-59	20.149305351913817	27.576242306597777	31.869181251627737	20.405271089860673
60-61	20.12169003657603	27.77788094252135	31.747918187057074	20.352510833845546
62-63	19.97730479508548	27.59160330032166	32.083167882927036	20.34792402166583
64-65	20.008777293056205	27.575354809537227	32.06034729421217	20.355520603194396
66-67	19.961038090844788	27.584421983256806	31.95295911970659	20.501580806191814
68-69	20.313982173911292	27.79476198095575	31.5810494669474	20.31020637818556
70-71	20.220354580878617	27.60162489026606	31.540889734349847	20.63713079450548
72-73	20.101918393792605	27.772089166137565	31.587150501785068	20.53884193828476
74-75	20.219826337564715	27.88338693743547	31.33408911403437	20.562697610965444
76-77	20.266516624560172	27.74718957331157	31.286146824000422	20.700146978127833
78-79	20.273350352349972	27.767412343475883	31.438803265088595	20.520434039085554
80-81	20.166643428898077	27.898615034150236	31.33115584351475	20.603585693436937
82-83	20.088800332026334	27.92253894918369	31.48787599398218	20.500784724807797
84-85	19.990749221738433	27.87373384034655	31.30305212236344	20.83246481555158
86-87	20.16252930937578	27.825522864408082	31.18078097261322	20.831166853602916
88-89	19.981291085333698	27.753692263886997	31.318289859737302	20.946726791042007
90-91	19.890680295573592	27.799336660091452	31.162646098889425	21.14733694544553
92-93	19.911345363891765	27.8514056069531	31.163180084662162	21.074068944492975
94-95	19.81141024500265	27.697485753635583	31.343967008012008	21.147136993349765
96-97	19.836582778210378	27.91956401441483	31.05872707757939	21.1851261297954
98-99	19.710801633126014	27.819268584360017	31.079578373212374	21.390351409301587
100	19.735694544599227	27.885339712977892	30.973040224566788	21.40592551785609
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3533.0
1	2079.5
2	2859.0
3	5141.0
4	3214.5
5	1835.0
6	4515.5
7	11747.0
8	21122.0
9	23572.5
10	14979.0
11	6650.5
12	5186.0
13	5427.5
14	5948.5
15	6467.5
16	6941.5
17	7581.5
18	8361.5
19	9056.5
20	10184.0
21	12099.5
22	15586.5
23	21108.0
24	26873.5
25	32326.0
26	39746.5
27	48822.0
28	60261.5
29	70479.0
30	75475.5
31	80924.0
32	87119.5
33	93617.0
34	99828.0
35	103982.0
36	105680.5
37	105697.5
38	105086.0
39	103415.5
40	103431.0
41	102135.0
42	98314.0
43	97197.0
44	97412.5
45	95596.5
46	93649.0
47	91425.5
48	88583.0
49	87204.0
50	87648.0
51	84065.5
52	75611.5
53	69626.0
54	66664.0
55	64606.5
56	58521.5
57	50270.5
58	45545.0
59	42441.0
60	36915.5
61	30186.5
62	25376.5
63	20443.5
64	15953.0
65	12621.0
66	9700.5
67	7282.5
68	5490.0
69	4139.0
70	2913.5
71	2068.0
72	1497.5
73	1073.0
74	891.0
75	707.0
76	412.0
77	284.5
78	225.0
79	115.5
80	43.0
81	24.0
82	15.5
83	11.0
84	4.0
85	1.0
86	2.0
87	2.0
88	1.0
89	0.0
90	0.5
91	0.5
92	1.5
93	2.0
94	1.0
95	1.0
96	0.5
97	1.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00541607863527198
2	0.004332862908217584
3	0.0010522667062814132
4	0.0
5	0.04295724083290005
6	0.0
7	0.0
8	0.0
9	3.0949020772982745E-5
10-11	0.0
12-13	1.5474510386491372E-5
14-15	1.5474510386491372E-5
16-17	0.0
18-19	7.737255193245685E-5
20-21	0.0
22-23	3.0949020772982745E-5
24-25	6.189804154596549E-5
26-27	1.547451038649137E-4
28-29	7.737255193245685E-5
30-31	3.094902077298274E-4
32-33	0.0012379608309193097
34-35	8.820470920300081E-4
36-37	0.0011915372997598356
38-39	8.510980712570253E-4
40-41	1.8569412463789644E-4
42-43	6.808784570056204E-4
44-45	2.4759216618386196E-4
46-47	2.630666765703533E-4
48-49	1.8569412463789644E-4
50-51	4.3328629082175845E-4
52-53	7.427764985515858E-4
54-55	0.001083215727054396
56-57	0.0023985491099061625
58-59	7.273019881650945E-4
60-61	0.001299858872465275
62-63	0.0012689098516922926
64-65	0.0017176706529005421
66-67	4.797098219812326E-4
68-69	4.023372700487756E-4
70-71	2.940156973433361E-4
72-73	1.702196142514051E-4
74-75	7.427764985515858E-4
76-77	8.35623560870534E-4
78-79	8.820470920300081E-4
80-81	9.12996112802991E-4
82-83	0.0015784000594221198
84-85	0.001253435341305801
86-87	0.0022283294956547575
88-89	0.0012379608309193097
90-91	0.0014391294659436974
92-93	0.0013153333828517667
94-95	0.001005843175121939
96-97	1.2379608309193098E-4
98-99	4.951843323677239E-4
100	3.094902077298274E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3231120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.6382448674317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47313096888443	37.62220169382155
2	16.748350860358443	17.63207587426416
3	5.619163313971365	8.873486834127412
4	2.3524077195607904	4.9530645428110995
5	1.1319971556679183	2.9793171734642043
6	0.6515777899845776	2.057874675563291
7	0.4231996290963593	1.5593539992926302
8	0.28522596743782064	1.2011035453233685
9	0.20719439709293705	0.9815714468404111
>10	0.9812894441459376	9.355219997996734
>50	0.0749534236759345	2.728253598336124
>100	0.04721081704707299	4.651566398944551
>500	0.0025908845947925133	0.9271954281648821
>1k	0.0012954422964590285	0.998825722266942
>5k	2.3553496299255064E-4	0.7372868101028316
>10k+	1.7665122224441298E-4	2.741602258679769
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37371	1.1565958553071383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31346	0.970128005149917	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19735	0.6107789249548145	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6292	0.19473123870360742	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6212	0.1922553170417688	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6185	0.19141969348089827	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5098	0.15777810790066601	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4341	0.1343496991755181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.5474510386491372E-5	0.0
14-15	0.0	0.0	0.0	4.642353115947412E-5	0.0
16-17	0.0	0.0	0.0	6.189804154596549E-5	0.0
18-19	0.0	0.0	0.0	1.2379608309193098E-4	0.0
20-21	0.0	0.0	0.0	2.940156973433361E-4	0.0
22-23	0.0	0.0	0.0	5.880313946866722E-4	0.0
24-25	0.0	0.0	0.0	9.903686647354478E-4	0.0
26-27	0.0	0.0	0.0	0.0016867216321275595	0.0
28-29	0.0	0.0	0.0	0.003543662878506524	0.0
30-31	0.0	0.0	0.0	0.008216965015226918	0.0
32-33	0.0	0.0	0.0	0.015428086855331896	0.0
34-35	0.0	0.0	0.0	0.026925648072494986	0.0
36-37	0.0	0.0	0.0	0.04504629973507638	0.0
38-39	0.0	0.0	0.0	0.0703161751962168	0.0
40-41	0.0	0.0	0.0	0.09821671742306073	0.0
42-43	0.0	0.0	0.0	0.12617915769145066	0.0
44-45	0.0	0.0	0.0	0.15938745698086113	0.0
46-47	0.0	0.0	0.0	0.20181856446062046	0.0
48-49	0.0	0.0	0.0	0.25040852707420336	0.0
50-51	0.0	0.0	0.0	0.29923060734358364	0.0
52-53	0.0	0.0	0.0	0.34693852286513654	0.0
54-55	0.0	0.0	0.0	0.4020742033722053	0.0
56-57	0.0	0.0	0.0	0.47129168833098123	0.0
58-59	0.0	0.0	0.0	0.5483238010349353	0.0
60-61	0.0	0.0	0.0	0.6332943375671594	0.0
62-63	0.0	0.0	0.0	0.7196885290549406	0.0
64-65	0.0	0.0	0.0	0.8116535442818589	0.0
66-67	0.0	0.0	0.0	0.9217392111713585	0.0
68-69	0.0	0.0	0.0	1.0531487533734434	0.0
70-71	0.0	0.0	0.0	1.1983306098195055	0.0
72-73	0.0	0.0	0.0	1.3479072272153307	0.0
74-75	0.0	0.0	0.0	1.5022345192998094	0.0
76-77	0.0	0.0	0.0	1.6722684394265765	0.0
78-79	0.0	0.0	0.0	1.8754951843323677	0.0
80-81	0.0	0.0	0.0	2.1004171928000197	0.0
82-83	0.0	0.0	0.0	2.34462044120924	0.0
84-85	0.0	0.0	0.0	2.588591571962663	0.0
86-87	0.0	0.0	0.0	2.8506214563371213	0.0
88	0.0	0.0	0.0	3.0657790487508976	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11905	0.0	67.38175	1
GTATCAA	24060	0.0	46.2589	1
GTGGTAT	3535	0.0	41.89998	1
TGGTATC	3575	0.0	41.29772	2
TATCAAC	31385	0.0	35.460808	2
ATCAACG	31495	0.0	35.246292	3
TCAACGC	31845	0.0	34.873135	4
CAACGCA	32830	0.0	33.81251	5
AACGCAG	33900	0.0	32.77369	6
CGCAGAG	38010	0.0	28.970232	8
ACGCAGA	38190	0.0	28.969057	7
GCAGAGT	40135	0.0	27.155325	9
CATGGGT	10010	0.0	27.101545	4
GTACATG	20180	0.0	26.003733	1
TACATGG	20075	0.0	25.693518	2
TACCTGG	2240	0.0	25.60851	2
ACATGGG	20100	0.0	24.842054	3
GAGTACT	28620	0.0	23.777878	12-13
CAGAGTA	39550	0.0	23.41572	10-11
ATGGGTG	5250	0.0	23.28447	5
>>END_MODULE