FastQCFastQC Report
Wed 25 May 2016
SRR2431418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460269
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192680.43199188210397177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.047.6951941
GTATCAA103350.037.6898731
TCAACGC112200.034.0007864
ATCAACG112800.033.7801253
CAACGCA122250.031.205635
TATCAAC124400.030.9330792
AACGCAG128150.029.9238996
GTACATG202250.026.307461
ACGCAGA144600.025.9996997
CGCAGAG143400.025.8237178
TACATGG202450.025.7926162
CATGGGT95500.024.7756794
ACATGGG205550.024.350593
GCAGAGT165200.021.676869
GTGGTAT28650.021.3463481
TACCTGG34700.020.6065042
GTACCTG38500.019.1842021
ATGGGTG56300.019.0496675
CAGAGTA158250.018.76818810-11
TGGTATC29700.018.5318872
Produced by FastQC (version 0.11.5)