##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431418_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4460269
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77259645102123	31.0	31.0	34.0	27.0	34.0
2	30.885948358720068	33.0	31.0	34.0	27.0	34.0
3	30.963193923953913	33.0	31.0	34.0	27.0	34.0
4	34.23627745322087	37.0	35.0	37.0	30.0	37.0
5	34.318657013736164	37.0	35.0	37.0	31.0	37.0
6	34.46873024922936	37.0	35.0	37.0	31.0	37.0
7	34.42364507611536	37.0	35.0	37.0	31.0	37.0
8	34.398174190839164	37.0	35.0	37.0	31.0	37.0
9	35.89916639556942	39.0	37.0	39.0	30.0	39.0
10-11	35.906794298729515	39.0	37.0	39.0	30.5	39.0
12-13	35.83986840255599	39.0	37.0	39.0	30.0	39.0
14-15	37.21030502868773	41.0	38.0	41.0	31.0	41.0
16-17	37.2343272345233	41.0	38.0	41.0	31.0	41.0
18-19	37.263445097145485	41.0	38.0	41.0	31.0	41.0
20-21	37.3581875891342	41.0	38.0	41.0	31.0	41.0
22-23	37.33836703571018	41.0	38.0	41.0	30.5	41.0
24-25	37.38521678849415	41.0	38.0	41.0	30.5	41.0
26-27	37.42386871733521	41.0	38.0	41.0	30.0	41.0
28-29	37.394495488949204	40.0	38.0	41.0	30.0	41.0
30-31	37.32738585946274	40.0	38.0	41.0	30.0	41.0
32-33	37.27877376902604	40.0	38.0	41.0	30.0	41.0
34-35	37.2094851229825	40.0	38.0	41.0	30.0	41.0
36-37	37.07706171982004	40.0	38.0	41.0	30.0	41.0
38-39	36.95957474762173	40.0	38.0	41.0	30.0	41.0
40-41	36.89885935130818	40.0	37.5	41.0	30.0	41.0
42-43	36.7387001770521	40.0	37.0	41.0	30.0	41.0
44-45	36.5864675426527	40.0	37.0	41.0	29.0	41.0
46-47	36.458217206181956	40.0	37.0	41.0	29.0	41.0
48-49	36.32534439066343	40.0	36.0	41.0	29.0	41.0
50-51	36.19648164718316	40.0	36.0	41.0	29.0	41.0
52-53	36.03879553004539	40.0	35.5	41.0	28.5	41.0
54-55	35.88162530107489	40.0	35.0	41.0	28.0	41.0
56-57	35.69371051387259	39.0	35.0	41.0	28.0	41.0
58-59	35.48471773787635	39.0	35.0	41.0	28.0	41.0
60-61	35.25538549356552	39.0	35.0	41.0	27.0	41.0
62-63	35.159839574698296	39.0	35.0	41.0	27.5	41.0
64-65	34.91866421061151	38.0	34.0	40.5	27.0	41.0
66-67	34.66530494012805	38.0	34.0	40.0	26.0	41.0
68-69	34.37879744472811	37.0	34.0	40.0	26.0	41.0
70-71	34.050992328041204	37.0	34.0	40.0	26.0	41.0
72-73	33.716015446601986	36.0	33.5	39.0	26.0	41.0
74-75	33.42776366178811	36.0	33.0	39.0	26.0	41.0
76-77	33.15661779592217	35.5	33.0	39.0	25.5	40.5
78-79	32.79835846223625	35.0	33.0	37.5	25.0	39.5
80-81	32.45973852698122	35.0	33.0	37.0	24.5	39.0
82-83	32.11816260409406	35.0	33.0	37.0	24.0	39.0
84-85	31.806363024292928	35.0	33.0	36.0	23.0	38.0
86-87	31.51406933079597	35.0	32.0	36.0	22.5	37.0
88-89	31.23589855230705	35.0	32.0	35.5	20.5	37.0
90-91	30.996955116384235	35.0	32.0	35.0	20.0	36.5
92-93	30.761268322605655	35.0	32.0	35.0	18.5	36.0
94-95	30.549253979972956	35.0	32.0	35.0	16.0	36.0
96-97	30.3456262391349	35.0	32.0	35.0	4.5	36.0
98-99	30.13666866280935	35.0	31.0	35.0	2.0	35.5
100	29.963529553934976	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32954825710607594
1101	2	0.34040679501444515
1101	3	0.3878784126917161
1101	4	0.45698740585880415
1101	5	0.42716847550647685
1101	6	0.4264240044058738
1101	7	0.456236751812483
1101	8	0.4042565732896648
1101	9	0.3577820805951575
1101	10-11	0.39281126331281513
1101	12-13	0.3796452057725048
1101	14-15	0.42504717994746954
1101	16-17	0.39681175316334816
1101	18-19	0.3348399938148816
1101	20-21	0.36070735758023886
1101	22-23	0.4207462927508274
1101	24-25	0.4053214854458176
1101	26-27	0.39373262780760143
1101	28-29	0.38823524135428755
1101	30-31	0.38892883738327555
1101	32-33	0.40611570233963334
1101	34-35	0.3834816348537018
1101	36-37	0.3802857056631126
1101	38-39	0.45892196075235603
1101	40-41	0.33439743882794204
1101	42-43	0.3613750342440767
1101	44-45	0.3979181810580954
1101	46-47	0.30004229920918135
1101	48-49	0.40466498013054775
1101	50-51	0.3539869767869206
1101	52-53	0.29682027906951447
1101	54-55	0.3174620128597496
1101	56-57	0.2589988484313608
1101	58-59	0.3170716945765406
1101	60-61	0.34833600280693844
1101	62-63	0.33409898162088325
1101	64-65	0.3257133348494534
1101	66-67	0.3251641941441079
1101	68-69	0.293870013537159
1101	70-71	0.2927332215458307
1101	72-73	0.27312735839485214
1101	74-75	0.33141896662992565
1101	76-77	0.3462017262763126
1101	78-79	0.2513073779239434
1101	80-81	0.22619801805573303
1101	82-83	0.32420462399101524
1101	84-85	0.2670362745838659
1101	86-87	0.23615529944381208
1101	88-89	0.27560674477213354
1101	90-91	0.2615309707971072
1101	92-93	0.25788780028275937
1101	94-95	0.22733339905097694
1101	96-97	0.2569886368268044
1101	98-99	0.19020363667408802
1101	100	0.2464764325717681
1102	1	-0.028076930008349876
1102	2	0.007447862672695038
1102	3	0.0406917304474419
1102	4	0.12552047662525467
1102	5	0.09381832280458013
1102	6	0.13807809869950205
1102	7	0.08072523685962807
1102	8	0.13682518711859615
1102	9	0.15549573641263237
1102	10-11	0.1693520505670847
1102	12-13	0.1496370448099924
1102	14-15	0.16078688292934373
1102	16-17	0.11635901422028638
1102	18-19	0.14105014721507558
1102	20-21	0.08632673397649882
1102	22-23	0.06827586843065347
1102	24-25	0.08279197652284864
1102	26-27	0.09701454341595195
1102	28-29	0.029345994301579026
1102	30-31	0.0829522699965608
1102	32-33	0.05654688360495186
1102	34-35	0.04426628787097542
1102	36-37	0.04799411514753871
1102	38-39	0.10057703397857409
1102	40-41	0.11138074845545276
1102	42-43	0.021848109088480783
1102	44-45	0.031903055415895665
1102	46-47	-0.013450964066905158
1102	48-49	-0.10196660803417501
1102	50-51	-0.10613723933015962
1102	52-53	-0.047356588506396236
1102	54-55	-0.13568836655346672
1102	56-57	-0.0604373367766442
1102	58-59	-0.12158237084789647
1102	60-61	-0.05778917289584484
1102	62-63	0.0653232887338504
1102	64-65	0.011971547139175698
1102	66-67	0.025590830905045436
1102	68-69	-0.016726783171691295
1102	70-71	0.025242484752183714
1102	72-73	-0.00873835865514394
1102	74-75	-0.023585195219119726
1102	76-77	0.00885645292109416
1102	78-79	-0.002268937933017412
1102	80-81	0.011975812750847581
1102	82-83	-0.01674030492905132
1102	84-85	-0.03320591676606455
1102	86-87	-0.04566679892919012
1102	88-89	-0.07078335185602214
1102	90-91	-0.042591758732985596
1102	92-93	-0.05650927523294769
1102	94-95	-0.040503604288733897
1102	96-97	-0.08403988930931305
1102	98-99	-0.12928155814181252
1102	100	-0.20617980756427912
1103	1	-0.27141623827972694
1103	2	-0.2812471567914514
1103	3	-0.24551244893102364
1103	4	-0.0749961353964892
1103	5	-0.12155800559724383
1103	6	-0.08757637738457902
1103	7	-0.11725786051687237
1103	8	-0.091881519378326
1103	9	-0.04902268992750436
1103	10-11	0.040808231964547304
1103	12-13	0.018728885709037968
1103	14-15	-0.06068717281232949
1103	16-17	0.008092507298393059
1103	18-19	-0.05885024466630284
1103	20-21	-0.13830510175463218
1103	22-23	-0.13255438161578326
1103	24-25	-0.0778966664426477
1103	26-27	-0.06776589772406538
1103	28-29	-0.15132225032439095
1103	30-31	-0.14047454660671832
1103	32-33	-0.0865363048522454
1103	34-35	-0.11020693541696147
1103	36-37	-0.1778957081343151
1103	38-39	-0.06307656642753301
1103	40-41	-0.027570303374993443
1103	42-43	-0.1535045544211684
1103	44-45	-0.251514072963019
1103	46-47	-0.2886371992927792
1103	48-49	-0.2622554143622864
1103	50-51	-0.32811528211995267
1103	52-53	-0.3994894145221721
1103	54-55	-0.4015338702564364
1103	56-57	-0.3770213177363928
1103	58-59	-0.3560338650896071
1103	60-61	-0.358620819609655
1103	62-63	-0.2802677871462649
1103	64-65	-0.3229859054754485
1103	66-67	-0.36885238427222333
1103	68-69	-0.39648988739710944
1103	70-71	-0.4211710982902801
1103	72-73	-0.3611160636862891
1103	74-75	-0.3526958583972686
1103	76-77	-0.3565960075945682
1103	78-79	-0.3954287931853102
1103	80-81	-0.3939858606975264
1103	82-83	-0.38795414329931077
1103	84-85	-0.42028775792645945
1103	86-87	-0.49848615067264745
1103	88-89	-0.47573711367091676
1103	90-91	-0.5697410986539921
1103	92-93	-0.5597791134189123
1103	94-95	-0.5859351577681267
1103	96-97	-0.43993657150222276
1103	98-99	-0.5546690633805653
1103	100	-0.5371954725755828
1104	1	-0.15655160699104798
1104	2	-0.12027522512571309
1104	3	-0.08803877554911566
1104	4	0.032996807357136504
1104	5	-0.021277674831722493
1104	6	-0.02075637655551077
1104	7	0.00450297251317977
1104	8	-0.020964493852581256
1104	9	0.06824224403656842
1104	10-11	0.010885966611866138
1104	12-13	-0.021213035940462532
1104	14-15	-0.018868589421913384
1104	16-17	0.03177027306297475
1104	18-19	-0.08110844499766756
1104	20-21	-0.09692784213243044
1104	22-23	0.009868256844121959
1104	24-25	0.00393755032499854
1104	26-27	-0.011454366687225104
1104	28-29	0.003956396971481979
1104	30-31	-0.039296262917666525
1104	32-33	-0.09891138031579771
1104	34-35	-0.07571590752093726
1104	36-37	-0.14776365448378215
1104	38-39	-0.1458140346101615
1104	40-41	-0.1056415585696655
1104	42-43	-0.18518904147580884
1104	44-45	-0.21691292004078377
1104	46-47	-0.28152243170625013
1104	48-49	-0.23172178177605218
1104	50-51	-0.2571455913104117
1104	52-53	-0.2926949634859568
1104	54-55	-0.19925257587849643
1104	56-57	-0.19667346673322328
1104	58-59	-0.2671800445920738
1104	60-61	-0.30429297738206174
1104	62-63	-0.2981308335098589
1104	64-65	-0.253014334893912
1104	66-67	-0.31513946209494037
1104	68-69	-0.26450768659111645
1104	70-71	-0.30279919759428964
1104	72-73	-0.2897407862653907
1104	74-75	-0.21206640892454942
1104	76-77	-0.20332960687037627
1104	78-79	-0.25226268171694954
1104	80-81	-0.23845951200034676
1104	82-83	-0.30988387158332387
1104	84-85	-0.3360033146464545
1104	86-87	-0.2572029066957313
1104	88-89	-0.32944399483034914
1104	90-91	-0.32945802791684287
1104	92-93	-0.3571290882934903
1104	94-95	-0.3205537477675158
1104	96-97	-0.3204091694010103
1104	98-99	-0.29480980016444036
1104	100	-0.27448987451161955
1105	1	-0.28749416234256486
1105	2	-0.28373418747963797
1105	3	-0.2551520278169832
1105	4	-0.07956128262914319
1105	5	-0.21280928336285143
1105	6	-0.15913538089720447
1105	7	-0.09176835258725902
1105	8	-0.05376661115178649
1105	9	-0.1455518847795858
1105	10-11	-0.13611213782365184
1105	12-13	-0.13726126925907778
1105	14-15	-0.20784971091331528
1105	16-17	-0.21999078526256
1105	18-19	-0.14999526159540721
1105	20-21	-0.20317760356832082
1105	22-23	-0.14647689527617302
1105	24-25	-0.24197142616976208
1105	26-27	-0.21606740284583736
1105	28-29	-0.22729822616906148
1105	30-31	-0.21927652144292864
1105	32-33	-0.26565380477925515
1105	34-35	-0.3459008673827242
1105	36-37	-0.3896357342509873
1105	38-39	-0.3292140276512612
1105	40-41	-0.3164521017305262
1105	42-43	-0.37320962515573797
1105	44-45	-0.36813718106709814
1105	46-47	-0.34126811023854486
1105	48-49	-0.5014825646873788
1105	50-51	-0.38370035120079393
1105	52-53	-0.3269279470795752
1105	54-55	-0.3971142866453903
1105	56-57	-0.4376235768060752
1105	58-59	-0.3214009820087824
1105	60-61	-0.42990656188550247
1105	62-63	-0.3114377863636122
1105	64-65	-0.32781163405993397
1105	66-67	-0.3474327671882236
1105	68-69	-0.3657990518009484
1105	70-71	-0.30843595383213795
1105	72-73	-0.2562974062023784
1105	74-75	-0.3524671710855145
1105	76-77	-0.41119374601806413
1105	78-79	-0.37288530591671787
1105	80-81	-0.3644402591917242
1105	82-83	-0.31839233097355546
1105	84-85	-0.3358641366708355
1105	86-87	-0.41407676392386605
1105	88-89	-0.3584784688435789
1105	90-91	-0.39497847120110663
1105	92-93	-0.38112384630887775
1105	94-95	-0.4372426530910225
1105	96-97	-0.436222895546738
1105	98-99	-0.47536245584394266
1105	100	-0.38423544574280655
1106	1	-0.1644604386807771
1106	2	-0.11234347340402451
1106	3	-0.1010092405780938
1106	4	0.027433774641011155
1106	5	-0.024145401764542385
1106	6	0.12371078692336823
1106	7	0.09267818232973468
1106	8	0.06601716950623171
1106	9	0.11547117192034762
1106	10-11	0.06456007614849568
1106	12-13	0.07084638528421294
1106	14-15	0.11178966916046562
1106	16-17	0.025222057816030485
1106	18-19	-0.00183050508802296
1106	20-21	-0.02664384825625632
1106	22-23	0.027168240141868694
1106	24-25	-0.0119231591475355
1106	26-27	-0.11482778339959054
1106	28-29	-0.06091921521001353
1106	30-31	-0.023997615137993478
1106	32-33	-0.0709752946244322
1106	34-35	-0.05208614777259157
1106	36-37	-0.09306482062564214
1106	38-39	-0.06833128015838241
1106	40-41	-0.022730414750682826
1106	42-43	-0.08232962068680649
1106	44-45	-0.100810432644316
1106	46-47	-0.1113731119600061
1106	48-49	-0.07575218368307901
1106	50-51	-0.13426999769223613
1106	52-53	-0.17387052103349276
1106	54-55	-0.18689814590276654
1106	56-57	-0.16488995297014242
1106	58-59	-0.2563717933426801
1106	60-61	-0.20794294038088879
1106	62-63	-0.2272400862462547
1106	64-65	-0.1633076738922199
1106	66-67	-0.16153712682780963
1106	68-69	-0.13487254224534695
1106	70-71	-0.18576790431585977
1106	72-73	-0.14370315072050488
1106	74-75	-0.15634602355924443
1106	76-77	-0.10463632808924217
1106	78-79	-0.07575864104317276
1106	80-81	-0.135216618713784
1106	82-83	-0.09477669604585515
1106	84-85	-0.04087184198088778
1106	86-87	-0.1186022177404027
1106	88-89	-0.1472880352834487
1106	90-91	-0.0907704488362242
1106	92-93	-0.12944649862916435
1106	94-95	-0.18677370032778384
1106	96-97	-0.18377366008688156
1106	98-99	-0.22868978178484767
1106	100	-0.1931835472899941
1107	1	-0.28111767914752406
1107	2	-0.31573881174522
1107	3	-0.22911141315098504
1107	4	-0.12390932119079423
1107	5	-0.07288849516249485
1107	6	-0.06248740092343752
1107	7	-0.09057996119210543
1107	8	-0.1596764104071795
1107	9	-0.059140586711492915
1107	10-11	-0.023876993428793014
1107	12-13	0.030030901076997907
1107	14-15	-0.024994690587561763
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2216	54-55	0.19297534894727875
2216	56-57	0.20378425483667684
2216	58-59	0.24492578044796431
2216	60-61	0.2749414064325393
2216	62-63	0.23676371873932567
2216	64-65	0.19075522145122648
2216	66-67	0.134881209390187
2216	68-69	0.16762884805471856
2216	70-71	0.07348489865009356
2216	72-73	0.0856393081825999
2216	74-75	0.1462803666996848
2216	76-77	0.15826448844675411
2216	78-79	0.11553275550469522
2216	80-81	0.13437556188252842
2216	82-83	0.13632947242608395
2216	84-85	0.14468052457471003
2216	86-87	0.12837272110229492
2216	88-89	0.16019702001906921
2216	90-91	0.17512349336012534
2216	92-93	0.16256750845894175
2216	94-95	0.11556199830408076
2216	96-97	0.06707775817639927
2216	98-99	0.0886346513125531
2216	100	0.11232353630302327
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93380.0
3	17188.0
4	12476.0
5	10797.0
6	7535.0
7	6684.0
8	7457.0
9	8149.0
10	8540.0
11	8975.0
12	10152.0
13	11505.0
14	12398.0
15	12957.0
16	12769.0
17	12716.0
18	12791.0
19	12374.0
20	12185.0
21	12643.0
22	15137.0
23	21630.0
24	34591.0
25	47003.0
26	53367.0
27	42660.0
28	39283.0
29	48560.0
30	58155.0
31	65643.0
32	83760.0
33	112282.0
34	158721.0
35	246920.0
36	420243.0
37	788264.0
38	1257941.0
39	659754.0
40	2684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6068453158327	21.727530862561633	14.555863667734743	22.109760153870926
2	14.738823818983843	22.246384199848276	40.71623857072083	22.29855341044705
3	17.237386745236837	28.44619147975143	30.829440724345336	23.486981050666394
4	11.703557606079466	19.479862346492375	39.52862454967615	29.287955497751998
5	12.166849016177407	40.04604147326425	35.05133451315825	12.735774997400087
6	27.71128129682984	40.25220323893344	19.10889505203209	12.927620412204638
7	24.6374476819403	35.22729159496095	23.39588240547074	16.739378317628006
8	23.647226476074156	37.32320963934564	21.362754683337677	17.66680920124252
9	24.638504039229577	18.554096916833217	21.857513936132815	34.94988510780439
10-11	22.04885348721507	29.20343429391628	29.62451502966333	19.123197189205317
12-13	22.949394622330264	27.41246338735533	29.039876512602824	20.598265477711582
14-15	20.9187854943697	29.00034573111811	26.557164206127542	23.52370456838465
16-17	20.08008256832263	31.896389363349638	27.245942393092655	20.777585675235077
18-19	20.09776740457571	30.836521892667644	29.09022827388828	19.975482428868364
20-21	21.844875730341414	29.82361870625299	28.331929600045946	19.999575963359646
22-23	21.44397411182574	29.8960401009816	28.301221774200336	20.35876401299232
24-25	21.045913359446637	29.94950017308906	28.40409419006323	20.600492277401074
26-27	20.79669863686172	29.781315878510267	28.490287785602554	20.93169769902546
28-29	20.698046876358408	29.872171804971	28.630403862879184	20.799377455791408
30-31	20.967336624224327	30.21472760850528	28.29935904584055	20.51857672142984
32-33	20.67084555028767	30.00002355796375	28.6539439452836	20.675186946464976
34-35	20.891925085122384	29.72597502598095	28.539040526690822	20.84305936220584
36-37	21.040173529795197	29.931971186371083	28.23797115361098	20.789884130222738
38-39	20.93901056793849	29.952402262639843	28.395836098284665	20.712751071137003
40-41	20.863097094440665	29.73716648844682	28.55452113756164	20.845215279550878
42-43	20.786416391120195	29.725822942892382	28.567125453313864	20.92063521267356
44-45	20.923348888308457	29.83585372399389	28.429084392696254	20.811712995001404
46-47	20.970395345753364	29.891921012072924	28.347909827449225	20.78977381472449
48-49	20.884907658458417	29.683576250195078	28.394183996915164	21.037332094431346
50-51	20.805410916003378	29.712481139641874	28.407926056738198	21.074181887616554
52-53	20.87616776952193	29.712124542038243	28.375146025530867	21.03656166290896
54-55	21.015582946334433	29.93209596899611	28.202218172916428	20.850102911753034
56-57	20.919538682538267	29.705055005954094	28.225803466107102	21.14960284540054
58-59	20.849299085080244	29.512854314034563	28.407097219339526	21.230749381545657
60-61	21.029694079762198	29.82367807921652	28.10674718994231	21.039880651078967
62-63	20.91248947775566	29.763547894384484	28.2849594276617	21.03900320019815
64-65	20.950942905172138	29.53599339481199	28.39750712486419	21.115556575151682
66-67	20.830078682618353	29.646337117085647	28.21675878407932	21.306825416216682
68-69	21.189696168258646	29.711812826975514	28.058386345677427	21.04010465908841
70-71	21.065746079465146	29.505934085657238	28.254930114643393	21.173389720234223
72-73	20.8985736632194	29.644517905347236	28.413979233397963	21.042929198035402
74-75	20.964194741496335	29.642012478516406	28.22271837543936	21.1710744045479
76-77	20.9965236425513	29.62656866871709	28.162691015677936	21.214216673053677
78-79	21.051669778740788	29.635351666245054	28.28993724061764	21.02304131439652
80-81	20.908948327809178	29.596617564039395	28.346423478074346	21.14801063007709
82-83	20.871003107223444	29.579396200607082	28.453644749761708	21.095955942407766
84-85	20.833898497521524	29.51160053488373	28.3007075126392	21.353793454955543
86-87	21.090987295778127	29.57296622898967	28.20629840679201	21.129748068440193
88-89	20.982890525571317	29.411997005864414	28.264536725729638	21.340575742834627
90-91	20.85986292901994	29.423601832250164	28.279006420504327	21.43752881822557
92-93	20.95422944664178	29.55416523545113	28.184068490141833	21.30753682776526
94-95	20.78986719255677	29.40893452286803	28.332026460528514	21.469171824046683
96-97	20.790543939953974	29.50196281449482	28.259772426817225	21.447720818733984
98-99	20.83104097877332	29.49085720747686	28.22488289919952	21.453218914550295
100	20.860409863875358	29.495339844864944	28.168694564169584	21.475555727090114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17609.0
1	12685.0
2	6853.5
3	5001.0
4	3434.0
5	2615.5
6	2284.5
7	2328.5
8	2749.5
9	2813.0
10	2239.5
11	1737.5
12	1582.0
13	1582.5
14	1736.0
15	1944.5
16	2155.0
17	2650.5
18	3614.5
19	4781.5
20	6534.0
21	9295.5
22	13584.0
23	19955.0
24	27502.5
25	36099.0
26	47248.5
27	60118.0
28	75381.5
29	91501.5
30	103899.0
31	114694.5
32	125038.5
33	134558.5
34	142937.0
35	149282.0
36	152935.5
37	155114.0
38	155641.0
39	154135.0
40	152904.5
41	150857.5
42	146212.0
43	142066.0
44	138085.0
45	132951.5
46	130225.0
47	127164.0
48	123396.0
49	120846.5
50	118067.0
51	113366.0
52	107662.0
53	102902.5
54	99401.5
55	95616.5
56	88096.5
57	79033.0
58	72056.5
59	65830.5
60	57817.0
61	48987.5
62	41615.0
63	34127.5
64	27000.0
65	21825.5
66	17216.5
67	13050.0
68	10063.5
69	7756.5
70	5748.5
71	4369.0
72	3419.5
73	2561.0
74	2023.0
75	1630.0
76	1139.0
77	851.0
78	645.5
79	479.0
80	383.0
81	284.5
82	221.0
83	184.5
84	142.0
85	131.0
86	118.5
87	96.5
88	93.5
89	82.5
90	71.0
91	54.5
92	44.0
93	41.5
94	31.5
95	31.0
96	28.5
97	20.5
98	18.0
99	20.5
100	35.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17276984863468997
2	0.16705270466870945
3	0.17998914415251635
4	0.17129011725525972
5	0.18375573311833884
6	0.07416593035083759
7	0.07611648535099565
8	0.06436831500521605
9	0.058561490349573087
10-11	0.07099347595402879
12-13	0.07026482035052145
14-15	0.06833668552277901
16-17	0.06433468474659264
18-19	0.06854967716072731
20-21	0.06965947569529998
22-23	0.06774255095376534
24-25	0.06784344172963559
26-27	0.07176697190236733
28-29	0.06895324026420828
30-31	0.0744349724198249
32-33	0.0712400978506005
34-35	0.07078048431608049
36-37	0.08134038552383276
38-39	0.0771141830234903
40-41	0.07234989638517318
42-43	0.07551114069577418
44-45	0.08557779811038303
46-47	0.07703571242003565
48-49	0.08148611664453421
50-51	0.0784818135408425
52-53	0.07686756112691857
54-55	0.07932257000642787
56-57	0.07289919060935562
58-59	0.08057809966170203
60-61	0.07669940983380151
62-63	0.06826942500553218
64-65	0.07099347595402879
66-67	0.07141945922992537
68-69	0.07019755983327462
70-71	0.06656549190194581
72-73	0.06437952509142386
74-75	0.06924470250561121
76-77	0.07409866983359076
78-79	0.07099347595402879
80-81	0.0780446201787381
82-83	0.07974855328232446
84-85	0.08070141060998788
86-87	0.07720386371315273
88-89	0.07974855328232446
90-91	0.07743927552351663
92-93	0.06954737483322193
94-95	0.06869540828142877
96-97	0.06774255095376534
98-99	0.07678909052346394
100	0.0844119491447713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4460269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.60008446793279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.278599037702	49.27603940931125
2	14.828420791508249	19.15834471314967
3	4.590237784297595	8.895892457805637
4	1.7558397148055838	4.537095755543669
5	0.8357212960058499	2.6993833156814078
6	0.45998458782913415	1.7829025936625589
7	0.2873042320664649	1.2991914361641737
8	0.1834262430818075	0.9479480637376269
9	0.1309147729426408	0.7611394851175356
>10	0.6012824767513812	6.724066956795709
>50	0.03196439615968738	1.4343940000198054
>100	0.015645159183205505	1.687754787458854
>500	4.165313232885815E-4	0.17104489936401285
>1k	2.0826543695276874E-4	0.19275046930527892
>5k	0.0	0.0
>10k+	3.4710906158684755E-5	0.432051656882899
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19268	0.43199188210397177	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.484034483121982E-5	0.0	0.0	0.0
4	0.0	4.484034483121982E-5	0.0	2.242017241560991E-5	0.0
5	0.0	4.484034483121982E-5	0.0	6.726051724682973E-5	0.0
6	0.0	4.484034483121982E-5	0.0	6.726051724682973E-5	0.0
7	0.0	4.484034483121982E-5	0.0	8.968068966243965E-5	0.0
8	0.0	4.484034483121982E-5	0.0	8.968068966243965E-5	0.0
9	0.0	4.484034483121982E-5	0.0	8.968068966243965E-5	0.0
10-11	0.0	4.484034483121982E-5	0.0	8.968068966243965E-5	0.0
12-13	0.0	5.605043103902478E-5	0.0	8.968068966243965E-5	0.0
14-15	0.0	6.726051724682973E-5	0.0	1.1210086207804956E-4	0.0
16-17	0.0	6.726051724682973E-5	0.0	1.3452103449365947E-4	0.0
18-19	0.0	6.726051724682973E-5	0.0	1.3452103449365947E-4	0.0
20-21	0.0	6.726051724682973E-5	0.0	1.9057146553268424E-4	0.0
22-23	0.0	6.726051724682973E-5	0.0	3.026723276107338E-4	0.0
24-25	0.0	6.726051724682973E-5	0.0	6.613950862604924E-4	0.0
26-27	0.0	6.726051724682973E-5	0.0	0.0010537481035336658	0.0
28-29	0.0	6.726051724682973E-5	0.0	0.002040235689820502	0.0
30-31	0.0	6.726051724682973E-5	0.0	0.004966068190057595	0.0
32-33	0.0	6.726051724682973E-5	0.0	0.01088499370777861	0.0
34-35	0.0	6.726051724682973E-5	0.0	0.019527970173996233	0.0
36-37	0.0	6.726051724682973E-5	0.0	0.0346055361234939	0.0
38-39	0.0	6.726051724682973E-5	0.0	0.05538903595276429	0.0
40-41	0.0	8.968068966243965E-5	0.0	0.07750653604076346	0.0
42-43	0.0	8.968068966243965E-5	0.0	0.10003880931845141	0.0
44-45	0.0	8.968068966243965E-5	0.0	0.127593201217236	0.0
46-47	0.0	8.968068966243965E-5	0.0	0.16194090535795036	0.0
48-49	0.0	8.968068966243965E-5	0.0	0.20205059380947651	0.0
50-51	0.0	1.793613793248793E-4	0.0	0.2421154419161714	0.0
52-53	0.0	2.017815517404892E-4	0.0	0.2821018194194117	0.0
54-55	0.0	2.017815517404892E-4	0.0	0.32745782821619057	0.0
56-57	0.0	2.017815517404892E-4	0.0	0.38404634339318994	0.0
58-59	0.0	2.017815517404892E-4	0.0	0.44808956589837967	0.0
60-61	0.0	2.017815517404892E-4	0.0	0.5186570585765119	0.0
62-63	0.0	2.017815517404892E-4	0.0	0.5897850555650344	0.0
64-65	0.0	2.017815517404892E-4	0.0	0.6670673898816417	0.0
66-67	0.0	2.017815517404892E-4	0.0	0.7585865336821613	0.0
68-69	0.0	2.1299163794829417E-4	0.0	0.8640958650700216	0.0
70-71	0.0	2.2420172415609912E-4	0.0	0.9834048125796897	0.0
72-73	0.0	2.2420172415609912E-4	0.0	1.1081842821587666	0.0
74-75	0.0	2.2420172415609912E-4	0.0	1.2340511301000008	0.0
76-77	0.0	2.2420172415609912E-4	0.0	1.375589678559746	0.0
78-79	0.0	2.2420172415609912E-4	0.0	1.5413420132283502	0.0
80-81	0.0	2.2420172415609912E-4	0.0	1.7291445874677065	0.0
82-83	0.0	2.2420172415609912E-4	0.0	1.9322489293807168	0.0
84-85	0.0	2.2420172415609912E-4	0.0	2.1375504481904564	0.0
86-87	0.0	2.2420172415609912E-4	0.0	2.3578398522600317	0.0
88	0.0	2.2420172415609912E-4	0.0	2.5406315179644996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5780	0.0	47.695194	1
GTATCAA	10335	0.0	37.689873	1
TCAACGC	11220	0.0	34.000786	4
ATCAACG	11280	0.0	33.780125	3
CAACGCA	12225	0.0	31.20563	5
TATCAAC	12440	0.0	30.933079	2
AACGCAG	12815	0.0	29.923899	6
GTACATG	20225	0.0	26.30746	1
ACGCAGA	14460	0.0	25.999699	7
CGCAGAG	14340	0.0	25.823717	8
TACATGG	20245	0.0	25.792616	2
CATGGGT	9550	0.0	24.775679	4
ACATGGG	20555	0.0	24.35059	3
GCAGAGT	16520	0.0	21.67686	9
GTGGTAT	2865	0.0	21.346348	1
TACCTGG	3470	0.0	20.606504	2
GTACCTG	3850	0.0	19.184202	1
ATGGGTG	5630	0.0	19.049667	5
CAGAGTA	15825	0.0	18.768188	10-11
TGGTATC	2970	0.0	18.531887	2
>>END_MODULE