FastQCFastQC Report
Wed 25 May 2016
SRR2431418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460269
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295200.6618434897088046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244540.5482628962513247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154560.3465261848556668No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.11649521587150909No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48210.10808765121565539No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46210.10360361673253339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118200.064.16611
GTATCAA232900.044.618151
GTGGTAT37150.037.084941
ATCAACG299750.034.2400673
TATCAAC302650.034.132142
TCAACGC303000.033.9189764
TGGTATC39400.033.295242
CAACGCA312200.032.949565
AACGCAG323500.031.9034336
ACGCAGA361900.028.3884017
CGCAGAG361900.028.2325638
TATAACG3800.027.2215732
GCAGAGT389450.026.0905559
GTACATG201450.026.072021
CATGGGT96600.025.9895864
TACATGG199750.025.6574732
ACATGGG201300.024.5937443
GAGTACT274750.023.29800812-13
CAGAGTA385700.022.51200510-11
AGAGTAC372750.022.14674210-11
Produced by FastQC (version 0.11.5)